Experiment set5S199 for Pseudomonas sp. RS175
mixed carbon sources Trisodium citrate dihydrate 10 mM and L-tyrosine disodium salt 5 mM
Group: mixed carbon sourceMedia: MME_noCarbon + Trisodium citrate dihydrate (10 mM) + L-tyrosine disodium salt (5 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 11/17/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 5 genes in this experiment
For mixed carbon source Trisodium citrate dihydrate in Pseudomonas sp. RS175
For mixed carbon source Trisodium citrate dihydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
- Purine metabolism
- Pyrimidine metabolism
- Phenylalanine metabolism
- Glutathione metabolism
- Styrene degradation
- Metabolism of xenobiotics by cytochrome P450
- Drug metabolism - cytochrome P450
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-tyrosine degradation I | 5 | 5 | 2 |
| plastoquinol-9 biosynthesis I | 3 | 1 | 1 |
| 4-hydroxy-2-nonenal detoxification | 4 | 1 | 1 |
| superpathway of plastoquinol biosynthesis | 5 | 2 | 1 |
| pentachlorophenol degradation | 10 | 3 | 2 |
| vitamin E biosynthesis (tocopherols) | 7 | 1 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 1 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 5 | 1 |
| gliotoxin biosynthesis | 9 | 2 | 1 |
| glutathione-mediated detoxification II | 9 | 1 | 1 |
| indole glucosinolate activation (intact plant cell) | 12 | 3 | 1 |
| camalexin biosynthesis | 12 | 2 | 1 |