Experiment set5S115 for Escherichia coli ECRC101
JK42
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 5/7/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 8 genes in this experiment
For phage Phage in Escherichia coli ECRC101
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, bacterial | 1 |
| Polyamine Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Glycine, serine and threonine metabolism
- Cysteine metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Aminosugars metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| D-serine degradation | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| glycine degradation | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 3 |