Experiment set5IT096 for Escherichia coli BW25113

M63 no sulfur + 5 mM sulfate

Group: sulfur source
Media: M63_no_sulfur + Sodium sulfate (5 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Hans on 5/11/2015
Media components: 64 mM Potassium phosphate monobasic, 33 mM Ammonium chloride, 1 mM Magnesium chloride hexahydrate, 0.3 mM Thiamine HCl, 2 g/L D-Glucose, Sulfur free mineral mix (0.03 g/L Magnesium chloride hexahydrate, 0.015 g/L Nitrilotriacetic acid disodium salt, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.0013 g/L Zinc chloride, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Potassium aluminum chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate, 0.0025 mg/L Sodium tungstate dihydrate)

Specific Phenotypes

For 3 genes in this experiment

For sulfur source Sodium sulfate in Escherichia coli BW25113

For sulfur source Sodium sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Acetoin, butanediol metabolism	1
Arginine Biosynthesis extended	1
Branched-Chain Amino Acid Biosynthesis	1
Lysine Biosynthesis DAP Pathway	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine biosynthesis
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine biosynthesis
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-acetoin II	2	1	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
L-valine biosynthesis	4	4	1
L-arginine degradation II (AST pathway)	5	5	1
L-ornithine biosynthesis I	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
superpathway of (R,R)-butanediol biosynthesis	5	2	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
superpathway of L-threonine metabolism	18	16	1
aspartate superpathway	25	24	1