Experiment set5IT095 for Escherichia coli BW25113

Compare to:

M63 no sulfur + 5 mM sulfate

Group: sulfur source
Media: M63_no_sulfur + Sodium sulfate (5 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Hans on 5/11/2015
Media components: 64 mM Potassium phosphate monobasic, 33 mM Ammonium chloride, 1 mM Magnesium chloride hexahydrate, 0.3 mM Thiamine HCl, 2 g/L D-Glucose, Sulfur free mineral mix (0.03 g/L Magnesium chloride hexahydrate, 0.015 g/L Nitrilotriacetic acid disodium salt, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.0013 g/L Zinc chloride, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Potassium aluminum chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate, 0.0025 mg/L Sodium tungstate dihydrate)

Specific Phenotypes

For 6 genes in this experiment

For sulfur source Sodium sulfate in Escherichia coli BW25113

For sulfur source Sodium sulfate across organisms

SEED Subsystems

Subsystem #Specific
Acetoin, butanediol metabolism 1
Arginine Biosynthesis extended 1
Branched-Chain Amino Acid Biosynthesis 1
Heat shock dnaK gene cluster extended 1
Lysine Biosynthesis DAP Pathway 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to (R)-acetoin II 2 1 1
5-(methoxycarbonylmethoxy)uridine biosynthesis 3 3 1
pyruvate fermentation to (S)-acetoin 3 3 1
pyruvate fermentation to (R)-acetoin I 3 2 1
UTP and CTP dephosphorylation I 7 5 2
L-valine biosynthesis 4 4 1
L-ornithine biosynthesis I 5 5 1
L-arginine degradation II (AST pathway) 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
superpathway of (R,R)-butanediol biosynthesis 5 2 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of 2,3-butanediol biosynthesis 6 3 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 6 1
L-isoleucine biosynthesis III 7 4 1
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 1
L-isoleucine biosynthesis II 8 4 1
superpathway of branched chain amino acid biosynthesis 17 17 2
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 9 1
L-lysine biosynthesis I 9 9 1
L-arginine biosynthesis I (via L-ornithine) 9 9 1
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 8 1
L-arginine biosynthesis III (via N-acetyl-L-citrulline) 9 8 1
L-arginine biosynthesis II (acetyl cycle) 10 9 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 14 1
superpathway of arginine and polyamine biosynthesis 17 16 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 18 1
superpathway of L-threonine metabolism 18 16 1
aspartate superpathway 25 24 1