Experiment set5IT088 for Ralstonia solanacearum PSI07

water survival rep C; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days

Group: survival
Media: Water + Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days
Culturing: RalstoniaPSI07_ML2, flask, Aerobic, at 28 (C)
By: Brian Ingel on 6-Nov-21

Specific Phenotypes

For 11 genes in this experiment

For survival Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days in Ralstonia solanacearum PSI07

For survival Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days across organisms

SEED Subsystems

Subsystem	#Specific
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Isoleucine degradation	1
Leucine Degradation and HMG-CoA Metabolism	1
Protein chaperones	1
Proteolysis in bacteria, ATP-dependent	1
Terminal cytochrome C oxidases	1
Translation elongation factor G family	1
Universal GTPases	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Citrate cycle (TCA cycle)
• Oxidative phosphorylation
• Glutamate metabolism
• Valine, leucine and isoleucine degradation
• Valine, leucine and isoleucine biosynthesis
• Arginine and proline metabolism
• Pyruvate metabolism
• Butanoate metabolism
• Nitrogen metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation I	1	1	1
arsenite to oxygen electron transfer	2	2	1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA	3	3	1
L-alanine degradation II (to D-lactate)	3	3	1
pyruvate decarboxylation to acetyl CoA I	3	3	1
arsenite to oxygen electron transfer (via azurin)	3	2	1
ethene biosynthesis IV (engineered)	3	2	1
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	2	1
Fe(II) oxidation	6	2	1
4-aminobutanoate degradation V	7	3	1
L-glutamate degradation XI (reductive Stickland reaction)	7	2	1
L-glutamate degradation V (via hydroxyglutarate)	10	5	1
methylaspartate cycle	19	11	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	18	1