Experiment set5IT084 for Pseudomonas putida KT2440
Levulinic Acid carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + Levulinic Acid (20 mM), pH=7
Culturing: Putida_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Jayashree on 27-Feb-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 14 genes in this experiment
For carbon source Levulinic Acid in Pseudomonas putida KT2440
For carbon source Levulinic Acid across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Butanoate metabolism
- Fatty acid metabolism
- Synthesis and degradation of ketone bodies
- Geraniol degradation
- Benzoate degradation via CoA ligation
- Fatty acid elongation in mitochondria
- Purine metabolism
- Lysine degradation
- Benzoate degradation via hydroxylation
- Tryptophan metabolism
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Propanoate metabolism
- Ethylbenzene degradation
- Terpenoid biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: