Experiment set5IT082 for Pseudomonas simiae WCS417
Glycine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Glycine (20 mM), pH=7
Culturing: fluoroDangl_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/2/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 18 genes in this experiment
For nitrogen source Glycine in Pseudomonas simiae WCS417
For nitrogen source Glycine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Coenzyme B12 biosynthesis | 11 |
| Cobalamin synthesis | 8 |
| Glycine and Serine Utilization | 2 |
| Glycine cleavage system | 2 |
| Photorespiration (oxidative C2 cycle) | 2 |
| Ammonia assimilation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Porphyrin and chlorophyll metabolism
- Glycine, serine and threonine metabolism
- One carbon pool by folate
- Riboflavin metabolism
- Nitrogen metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: