Experiment set5IT082 for Pseudomonas putida KT2440
Levulinic Acid carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + Levulinic Acid (40 mM), pH=7
Culturing: Putida_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Jayashree on 27-Feb-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 17 genes in this experiment
For carbon source Levulinic Acid in Pseudomonas putida KT2440
For carbon source Levulinic Acid across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Fatty acid metabolism
- Synthesis and degradation of ketone bodies
- Geraniol degradation
- Tryptophan metabolism
- Biosynthesis of plant hormones
- Fatty acid elongation in mitochondria
- Urea cycle and metabolism of amino groups
- Purine metabolism
- Pyrimidine metabolism
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine biosynthesis
- Lysine degradation
- Arginine and proline metabolism
- Phenylalanine metabolism
- Benzoate degradation via hydroxylation
- Cyanoamino acid metabolism
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Propanoate metabolism
- Ethylbenzene degradation
- Styrene degradation
- Terpenoid biosynthesis
- Biosynthesis of unsaturated fatty acids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: