Experiment set5IT070 for Agrobacterium fabrum C58

R2A with Ceftazidime hydrate 4 mM

Group: stress
Media: R2A + Ceftazidime hydrate (4 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1B B10

Specific Phenotypes

For 21 genes in this experiment

For stress Ceftazidime hydrate in Agrobacterium fabrum C58

For stress Ceftazidime hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Manganese	3
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Multidrug Resistance Efflux Pumps	1
Peptidoglycan Biosynthesis	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• D-Glutamine and D-glutamate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tRNA processing	10	10	2
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)	8	8	1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)	8	7	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	11	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	1