Experiment set5IT068 for Agrobacterium fabrum C58

R2A with Azlocillin sodium salt 0.2 mM

Group: stress
Media: R2A + Azlocillin sodium salt (0.2 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1B H9

Specific Phenotypes

For 5 genes in this experiment

For stress Azlocillin sodium salt in Agrobacterium fabrum C58

For stress Azlocillin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	2
Beta-lactamase	1
Tn552	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Other glycan degradation
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitin degradation II (Vibrio)	6	2	2
chitin degradation III (Serratia)	7	2	1
peptidoglycan recycling II	10	2	1
peptidoglycan recycling I	14	8	1