Experiment set5IT043 for Agrobacterium fabrum C58
R2A with Potassium Chloride 800 mM
Group: stressMedia: R2A + Potassium Chloride (800 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1B D6
Specific Phenotypes
For 4 genes in this experiment
For stress Potassium Chloride in Agrobacterium fabrum C58
For stress Potassium Chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| Methionine Biosynthesis | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Cysteine metabolism
- Lysine degradation
- Arginine and proline metabolism
- Phenylalanine metabolism
- Selenoamino acid metabolism
- D-Arginine and D-ornithine metabolism
- D-Alanine metabolism
- Sulfur metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-alanine degradation | 1 | 1 | 1 |
| L-cysteine biosynthesis I | 2 | 2 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 3 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of seleno-compound metabolism | 19 | 8 | 1 |