Experiment set5IT041 for Agrobacterium fabrum C58

Compare to:

R2A with Sodium m-arsenite 0.8 mM

Group: stress
Media: R2A + Sodium m-arsenite (0.8 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1B A6

Specific Phenotypes

For 88 genes in this experiment

For stress Sodium m-arsenite in Agrobacterium fabrum C58

For stress Sodium m-arsenite across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter dipeptide (TC 3.A.1.5.2) 5
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 3
D-ribose utilization 3
Thiamin biosynthesis 3
Transport of Zinc 3
Arsenic resistance 2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 2
Glycogen metabolism 2
Heat shock dnaK gene cluster extended 2
L-Arabinose utilization 2
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 2
Transport of Manganese 2
Bacterial Chemotaxis 1
Bacterial RNA-metabolizing Zn-dependent hydrolases 1
Chitin and N-acetylglucosamine utilization 1
Cobalt-zinc-cadmium resistance 1
CytR regulation 1
D-Sorbitol(D-Glucitol) and L-Sorbose Utilization 1
Entner-Doudoroff Pathway 1
Experimental tye 1
Flagellar motility 1
Flagellum 1
Glutamate dehydrogenases 1
Heme and Siroheme Biosynthesis 1
Histidine Degradation 1
Homogentisate pathway of aromatic compound degradation 1
Maltose and Maltodextrin Utilization 1
Mannitol Utilization 1
Methionine Biosynthesis 1
Multidrug Resistance Efflux Pumps 1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 1
One-carbon metabolism by tetrahydropterines 1
Oxidative stress 1
Pentose phosphate pathway 1
Peptidoglycan Biosynthesis 1
Proline, 4-hydroxyproline uptake and utilization 1
Proteasome bacterial 1
Purine conversions 1
Queuosine-Archaeosine Biosynthesis 1
Respiratory dehydrogenases 1 1
Ribonucleotide reduction 1
Serine-glyoxylate cycle 1
Sex pheromones in Enterococcus faecalis and other Firmicutes 1
Transcription initiation, bacterial sigma factors 1
Trehalose Biosynthesis 1
Two cell division clusters relating to chromosome partitioning 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate degradation I 1 1 1
cis-cyclopropane fatty acid (CFA) biosynthesis 1 1 1
L-asparagine degradation I 1 1 1
L-proline degradation I 3 3 2
proline to cytochrome bo oxidase electron transfer 2 2 1
adenosine deoxyribonucleotides de novo biosynthesis I 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis I 2 2 1
UDP-α-D-glucose biosynthesis 2 2 1
glycogen degradation I 8 6 4
guanosine deoxyribonucleotides de novo biosynthesis II 4 3 2
adenosine deoxyribonucleotides de novo biosynthesis II 4 3 2
pseudouridine degradation 2 1 1
arsenate detoxification III 2 1 1
sterculate biosynthesis 2 1 1
pentose phosphate pathway (oxidative branch) I 3 3 1
adenine salvage 3 3 1
L-alanine degradation II (to D-lactate) 3 3 1
sorbitol biosynthesis II 3 3 1
glycogen degradation II 6 5 2
L-arginine degradation I (arginase pathway) 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
starch degradation I 3 1 1
ethene biosynthesis IV (engineered) 3 1 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 12 4
superpathway of adenosine nucleotides de novo biosynthesis II 7 6 2
glycogen biosynthesis I (from ADP-D-Glucose) 4 4 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 2
heme b biosynthesis II (oxygen-independent) 4 3 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
sucrose biosynthesis II 8 5 2
starch degradation III 4 2 1
starch degradation V 4 2 1
arsenic detoxification (bacteria) 4 2 1
ethene biosynthesis II (microbes) 4 1 1
L-ascorbate biosynthesis VI (plants, myo-inositol pathway) 4 1 1
glycogen biosynthesis II (from UDP-D-Glucose) 4 1 1
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 9 2
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 8 2
superpathway of adenosine nucleotides de novo biosynthesis I 5 5 1
sucrose degradation II (sucrose synthase) 5 4 1
glucose and glucose-1-phosphate degradation 5 4 1
mannitol cycle 5 4 1
starch biosynthesis 10 6 2
glucose degradation (oxidative) 5 2 1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation 5 2 1
L-ascorbate degradation V 5 1 1
Salmonella enterica serotype O:3,10 O antigen biosynthesis 5 1 1
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 5 1
superpathway of heme b biosynthesis from uroporphyrinogen-III 6 4 1
L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) 6 3 1
arsenate detoxification I 6 3 1
arsenic detoxification (plants) 6 2 1
Salmonella enterica serotype O:9,46 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:2 O antigen biosynthesis 6 1 1
(5R)-carbapenem carboxylate biosynthesis 6 1 1
Porphyromonas gingivalis O-LPS antigen biosynthesis 6 1 1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1) 6 1 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 4
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 6 1
L-Nδ-acetylornithine biosynthesis 7 5 1
stachyose degradation 7 4 1
L-ascorbate biosynthesis VIII (engineered pathway) 7 3 1
L-ascorbate degradation II (bacterial, aerobic) 7 2 1
4-aminobutanoate degradation V 7 2 1
L-glutamate degradation XI (reductive Stickland reaction) 7 1 1
pentose phosphate pathway 8 8 1
L-citrulline biosynthesis 8 7 1
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 7 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 2 1
Salmonella enterica serotype O:8 O antigen biosynthesis 8 2 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 17 2
Entner-Doudoroff pathway III (semi-phosphorylative) 9 7 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 5 1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) 9 2 1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis 9 1 1
L-glutamate degradation V (via hydroxyglutarate) 10 4 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 2
colanic acid building blocks biosynthesis 11 11 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 4
superpathway of L-citrulline metabolism 12 9 1
arsenic detoxification (yeast) 12 3 1
folate transformations I 13 8 1
superpathway of purine nucleotide salvage 14 13 1
succinoglycan biosynthesis 14 10 1
superpathway of glucose and xylose degradation 17 16 1
type I lipoteichoic acid biosynthesis (S. aureus) 17 5 1
superpathway of anaerobic sucrose degradation 19 13 1
methylaspartate cycle 19 12 1
superpathway of bacteriochlorophyll a biosynthesis 26 6 1
mycolate biosynthesis 205 21 3
superpathway of mycolate biosynthesis 239 22 3