Experiment set5IT021 for Agrobacterium fabrum C58

R2A with Sodium Chloride 700 mM

Group: stress
Media: R2A + Sodium Chloride (700 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 2B E3

Specific Phenotypes

For 7 genes in this experiment

For stress Sodium Chloride in Agrobacterium fabrum C58

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	2
Potassium homeostasis	2
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Cobalamin synthesis	1
Coenzyme B12 biosynthesis	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Glutathione-regulated potassium-efflux system and associated functions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
aminopropanol phosphate biosynthesis I	2	1	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	3	2
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
superpathway of L-threonine metabolism	18	11	1
adenosylcobalamin biosynthesis II (aerobic)	33	29	1
adenosylcobalamin biosynthesis I (anaerobic)	36	26	1