Experiment set5IT019 for Agrobacterium fabrum C58
R2A with Magnesium sulfate 400 mM
Group: stressMedia: R2A + Magnesium sulfate (400 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 2B C3
Specific Phenotypes
For 9 genes in this experiment
For stress Magnesium sulfate in Agrobacterium fabrum C58
For stress Magnesium sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Peptidoglycan Biosynthesis | 3 |
| Copper homeostasis: copper tolerance | 1 |
| Phosphate metabolism | 1 |
| Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Peptidoglycan biosynthesis
- Lipopolysaccharide biosynthesis
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Lysine degradation
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Keratan sulfate biosynthesis
- Glycerolipid metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Biotin metabolism
- Carotenoid biosynthesis - General
- Zeatin biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 3 | 3 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 3 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 3 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 11 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |