Experiment set59S85 for Escherichia coli BW25113

Compare to:

Outgrowth from conjugation with pRH23 in LB+Gent50

200 most important genes:

  gene name fitness t score description  
b1292 sapC -4.6 -4.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3035 tolC -4.5 -12.0 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1863 ruvC -4.4 -5.1 Holliday junction resolvase (NCBI) compare
b0737 tolQ -4.2 -5.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3839 tatC -4.1 -13.2 TatABCE protein translocation system subunit (NCBI) compare
b0726 sucA -4.0 -6.7 alpha-ketoglutarate decarboxylase (NCBI) compare
b0437 clpP -3.9 -10.2 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0222 lpcA -3.5 -6.1 phosphoheptose isomerase (NCBI) compare
b1293 sapB -3.4 -2.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2188 yejM -3.3 -14.2 predicted hydrolase, inner membrane (NCBI) compare
b0738 tolR -3.2 -5.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3613 yibP -3.2 -9.2 putative membrane protein (VIMSS) compare
b2498 upp -3.2 -5.6 uracil phosphoribosyltransferase (VIMSS) compare
b0053 surA -3.1 -10.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2894 xerD -3.1 -2.1 site-specific tyrosine recombinase XerD (NCBI) compare
b0912 ihfB -3.1 -7.6 integration host factor subunit beta (NCBI) compare
b0019 nhaA -2.9 -12.8 pH-dependent sodium/proton antiporter (NCBI) compare
b3405 ompR -2.9 -7.1 osmolarity response regulator (NCBI) compare
b3792 wzxE -2.9 -12.8 O-antigen translocase (NCBI) compare
b0739 tolA -2.8 -6.3 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3619 rfaD -2.8 -7.7 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2830 nudH -2.8 -2.6 dinucleoside polyphosphate hydrolase (NCBI) compare
b0149 mrcB -2.7 -19.7 penicillin-binding protein 1b (NCBI) compare
b0438 clpX -2.7 -10.3 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0890 ftsK -2.7 -6.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0740 tolB -2.7 -7.1 translocation protein TolB precursor (NCBI) compare
b3979 thrT -2.6 -0.9 tRNA-Thr (NCBI) compare
b0439 lon -2.6 -8.3 DNA-binding ATP-dependent protease La (NCBI) compare
b2168 fruK -2.6 -2.5 1-phosphofructokinase (NCBI) compare
b1861 ruvA -2.6 -3.0 Holliday junction DNA helicase motor protein (NCBI) compare
b3375 yhfR -2.5 -11.9 putative transcriptional regulator (VIMSS) compare
b1192 ldcA -2.5 -8.7 L,D-carboxypeptidase A (NCBI) compare
b0688 pgm -2.5 -5.6 phosphoglucomutase (NCBI) compare
b0114 aceE -2.5 -2.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b1290 sapF -2.4 -3.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3398 yrfF -2.4 -5.4 predicted inner membrane protein (NCBI) compare
b3621 rfaC -2.4 -9.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3620 rfaF -2.3 -8.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3052 rfaE -2.3 -8.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2846 yqeH -2.2 -1.2 orf, hypothetical protein (VIMSS) compare
b2512 yfgL -2.2 -7.6 protein assembly complex, lipoprotein component (NCBI) compare
b3290 trkA -2.2 -1.5 potassium transporter peripheral membrane component (NCBI) compare
b2297 pta -2.2 -1.5 phosphate acetyltransferase (NCBI) compare
b0741 pal -2.2 -5.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1830 prc -2.2 -8.5 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2912 ygfA -2.2 -4.8 putative ligase (VIMSS) compare
b3386 rpe -2.2 -1.5 ribulose-phosphate 3-epimerase (NCBI) compare
b3236 mdh -2.1 -10.7 malate dehydrogenase (NCBI) compare
b1236 galU -2.0 -6.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3150 yraP -2.0 -10.8 hypothetical protein (NCBI) compare
b2620 smpB -2.0 -1.9 SsrA-binding protein (NCBI) compare
b0630 lipB -1.9 -3.1 protein of lipoate biosynthesis (VIMSS) compare
b1105 ycfM -1.9 -4.8 predicted outer membrane lipoprotein (NCBI) compare
b4146 yjeK -1.9 -4.4 predicted lysine aminomutase (NCBI) compare
b3075 ebgR -1.9 -6.7 DNA-binding transcriptional repressor (NCBI) compare
b0730 mngR -1.8 -3.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b0200 gmhB -1.8 -2.4 hypothetical protein (NCBI) compare
b4239 treC -1.8 -4.8 trehalose-6-P hydrolase (NCBI) compare
b3791 rffA -1.8 -9.4 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2934 cmtB -1.8 -1.1 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1258 yciF -1.8 -1.7 hypothetical protein (NCBI) compare
b0051 ksgA -1.7 -6.4 dimethyladenosine transferase (NCBI) compare
b3438 gntR -1.7 -6.6 DNA-binding transcriptional repressor (NCBI) compare
b4481 rffT -1.7 -7.9 4-alpha-L-fucosyltransferase (NCBI) compare
b3204 ptsN -1.7 -5.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b0142 folK -1.7 -0.8 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3962 udhA -1.7 -7.5 putative oxidoreductase (VIMSS) compare
b3229 sspA -1.7 -5.5 stringent starvation protein A (NCBI) compare
b1860 ruvB -1.7 -1.6 Holliday junction DNA helicase B (NCBI) compare
b2064 asmA -1.7 -7.4 predicted assembly protein (NCBI) compare
b1712 ihfA -1.6 -2.2 integration host factor subunit alpha (NCBI) compare
b4155 yjeA -1.6 -1.1 putative lysyl-tRNA synthetase (VIMSS) compare
b3237 argR -1.6 -6.1 arginine repressor (NCBI) compare
b0088 murD -1.6 -2.7 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3609 secB -1.6 -3.2 export protein SecB (NCBI) compare
b0080 fruR -1.6 -5.9 DNA-binding transcriptional dual regulator (NCBI) compare
b0767 ybhE -1.6 -3.8 6-phosphogluconolactonase (NCBI) compare
b3838 tatB -1.5 -5.3 sec-independent translocase (NCBI) compare
b0018 mokC -1.5 -2.1 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1084 rne -1.5 -5.2 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b1748 astC -1.5 -5.7 succinylornithine transaminase, PLP-dependent (NCBI) compare
b3912 cpxR -1.5 -8.5 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b2808 gcvA -1.5 -5.8 DNA-binding transcriptional dual regulator (NCBI) compare
b2741 rpoS -1.5 -7.5 RNA polymerase sigma factor (NCBI) compare
b2518 ndk -1.5 -2.5 nucleoside diphosphate kinase (NCBI) compare
b4214 cysQ -1.4 -10.4 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2501 ppk -1.4 -7.6 polyphosphate kinase (NCBI) compare
b3194 yrbE -1.3 -7.5 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4676 yoaK -1.3 -0.6 expressed protein (RefSeq) compare
b3426 glpD -1.3 -5.1 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b4139 aspA -1.3 -8.7 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b4699 fnrS -1.3 -1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3728 pstS -1.3 -4.5 phosphate transporter subunit (NCBI) compare
b4175 hflC -1.3 -9.0 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2215 ompC -1.3 -7.3 outer membrane porin protein C (NCBI) compare
b0819 ybiS -1.3 -6.3 hypothetical protein (NCBI) compare
b0724 sdhB -1.3 -5.7 succinate dehydrogenase, FeS subunit (NCBI) compare
b1130 phoP -1.3 -1.5 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0720 gltA -1.3 -7.1 citrate synthase (NCBI) compare
b0928 aspC -1.3 -6.3 aspartate aminotransferase, PLP-dependent (NCBI) compare
b1747 astA -1.3 -6.7 arginine succinyltransferase (NCBI) compare
b4202 rpsR -1.3 -1.9 30S ribosomal protein S18 (NCBI) compare
b3811 xerC -1.3 -2.2 site-specific tyrosine recombinase XerC (NCBI) compare
b2313 cvpA -1.3 -5.7 membrane protein required for colicin V production (NCBI) compare
b3790 rffC -1.3 -5.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b4203 rplI -1.3 -4.0 50S ribosomal protein L9 (NCBI) compare
b0683 fur -1.2 -2.1 ferric uptake regulator (NCBI) compare
b2504 yfgG -1.2 -1.9 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3465 yhhF -1.2 -4.9 predicted methyltransferase (NCBI) compare
b3404 envZ -1.2 -7.6 osmolarity sensor protein (NCBI) compare
b2287 nuoB -1.2 -3.9 NADH dehydrogenase subunit B (NCBI) compare
b4603 rseX -1.2 -0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1677 lpp -1.2 -3.4 murein lipoprotein (NCBI) compare
b1745 astB -1.2 -6.4 succinylarginine dihydrolase (NCBI) compare
b1744 astE -1.2 -6.2 succinylglutamate desuccinylase (NCBI) compare
b0161 degP -1.2 -5.8 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b0685 ybfE -1.2 -3.1 orf, hypothetical protein (VIMSS) compare
b0082 mraW -1.2 -4.7 S-adenosyl-methyltransferase (NCBI) compare
b2762 cysH -1.1 -6.2 phosphoadenosine phosphosulfate reductase (NCBI) compare
b4172 hfq -1.1 -1.4 RNA-binding protein Hfq (NCBI) compare
b1175 minD -1.1 -4.9 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2318 truA -1.1 -2.5 tRNA pseudouridine synthase A (NCBI) compare
b3652 recG -1.1 -7.7 ATP-dependent DNA helicase (NCBI) compare
b4174 hflK -1.1 -7.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2017 yefM -1.1 -1.5 orf, hypothetical protein (VIMSS) compare
b3630 rfaP -1.1 -7.8 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3870 glnA -1.1 -2.9 glutamine synthetase (NCBI) compare
b3163 nlpI -1.1 -5.6 hypothetical protein (NCBI) compare
b2820 recB -1.1 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0677 nagA -1.1 -4.7 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0441 ppiD -1.1 -9.0 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b0585 fes -1.1 -4.6 enterobactin/ferric enterobactin esterase (NCBI) compare
b3725 pstB -1.1 -1.0 phosphate transporter subunit (NCBI) compare
b3199 yrbK -1.1 -2.1 hypothetical protein (NCBI) compare
b3631 rfaG -1.1 -8.5 glucosyltransferase I (NCBI) compare
b1746 astD -1.1 -5.9 succinylglutamic semialdehyde dehydrogenase (NCBI) compare
b2569 lepA -1.1 -3.7 GTP-binding protein LepA (NCBI) compare
b4345 mcrC -1.0 -1.0 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b3466 yhhL -1.0 -3.9 conserved inner membrane protein (NCBI) compare
b4593 ymgI -1.0 -0.9 hypothetical protein (RefSeq) compare
b3169 nusA -1.0 -2.4 transcription elongation factor NusA (NCBI) compare
b3794 rffM -1.0 -5.9 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4557 yidD -1.0 -2.7 hypothetical protein (NCBI) compare
b0949 uup -1.0 -4.1 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3027 ygiZ -1.0 -0.6 conserved inner membrane protein (NCBI) compare
b4179 vacB -1.0 -4.4 putative enzyme (VIMSS) compare
b3844 fre -1.0 -4.2 NAD(P)H-flavin reductase (NCBI) compare
b2952 yggT -1.0 -4.0 predicted inner membrane protein (NCBI) compare
b2277 nuoM -1.0 -4.6 NADH dehydrogenase subunit M (NCBI) compare
b0990 cspG -0.9 -1.3 DNA-binding transcriptional regulator (NCBI) compare
b1545 pinQ -0.9 -0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1249 cls -0.9 -5.1 cardiolipin synthetase (NCBI) compare
b3786 rffE -0.9 -7.7 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3026 qseC -0.9 -2.9 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2110 yehC -0.9 -1.2 predicted periplasmic pilin chaperone (NCBI) compare
b1160 elbA -0.9 -1.0 hypothetical protein (NCBI) compare
b0406 tgt -0.9 -4.3 queuine tRNA-ribosyltransferase (NCBI) compare
b3849 trkH -0.9 -6.1 potassium transporter (NCBI) compare
b3787 rffD -0.9 -6.6 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3784 rfe -0.9 -6.6 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1187 fadR -0.9 -2.8 fatty acid metabolism regulator (NCBI) compare
b3933 ftsN -0.9 -5.1 essential cell division protein (NCBI) compare
b0926 ycbK -0.9 -3.5 hypothetical protein (NCBI) compare
b1848 yebG -0.9 -1.5 conserved protein regulated by LexA (NCBI) compare
b2937 speB -0.9 -5.0 agmatinase (NCBI) compare
b3842 rfaH -0.9 -3.3 transcriptional activator RfaH (NCBI) compare
b4041 plsB -0.9 -2.0 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2051 wcaH -0.9 -2.0 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1129 phoQ -0.9 -1.8 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b2346 vacJ -0.9 -5.6 predicted lipoprotein (NCBI) compare
b4458 oxyS -0.9 -1.5 global regulatory RNA (NCBI) compare
b2764 cysJ -0.9 -5.2 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b2363 yfdT -0.9 -2.6 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1176 minC -0.9 -3.0 septum formation inhibitor (NCBI) compare
b3437 gntK -0.8 -3.7 gluconokinase 2, thermoresistant (VIMSS) compare
b1423 ydcJ -0.8 -3.5 hypothetical protein (NCBI) compare
b3247 cafA -0.8 -6.6 bundles of cytoplasmic filaments (VIMSS) compare
b2513 yfgM -0.8 -4.6 hypothetical protein (NCBI) compare
b3195 yrbF -0.8 -6.7 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2340 sixA -0.8 -2.6 phosphohistidine phosphatase (NCBI) compare
b3846 fadB -0.8 -6.4 fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (NCBI) compare
b0125 hpt -0.8 -4.7 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b2175 spr -0.8 -2.8 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0721 sdhC -0.8 -2.6 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b1693 aroD -0.8 -4.7 3-dehydroquinate dehydratase (NCBI) compare
b1291 sapD -0.8 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0564 appY -0.8 -0.8 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0407 yajC -0.8 -2.1 preprotein translocase subunit YajC (NCBI) compare
b3601 mtlR -0.8 -4.7 DNA-binding repressor (NCBI) compare
b3390 aroK -0.8 -1.5 shikimate kinase I (NCBI) compare
b0989 cspH -0.8 -2.0 stress protein, member of the CspA-family (NCBI) compare
b1235 rssB -0.8 -3.8 response regulator of RpoS (NCBI) compare
b3727 pstC -0.8 -4.4 phosphate transporter subunit (NCBI) compare
b4178 yjeB -0.8 -4.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3389 aroB -0.8 -5.8 3-dehydroquinate synthase (NCBI) compare
b3281 aroE -0.8 -6.6 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3463 ftsE -0.8 -0.5 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2750 cysC -0.8 -4.0 adenylylsulfate kinase (NCBI) compare
b2276 nuoN -0.8 -3.0 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3469 zntA -0.7 -4.8 zinc, cobalt and lead efflux system (NCBI) compare


Specific Phenotypes

For 8 genes in this experiment

For dart Plasmid=pRH23; outgrowth=LB_Gent50 in Escherichia coli BW25113

For dart Plasmid=pRH23; outgrowth=LB_Gent50 across organisms