Experiment set58S337 for Escherichia coli BW25113

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SIR2-HerA

Group: sir2-hera
Media: LB + Kanamycin sulfate (0.05 mg/mL) + Chloramphenicol (34 0.034)
Culturing: Keio_ML9a, bioassay_dish, Aerobic, at 37 (C), (Solid)
By: Kate Miller/Brady Cress/Ben Adler on 10-Apr-23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 16 genes in this experiment

For sir2-hera Kanamycin sulfate in Escherichia coli BW25113

For sir2-hera Kanamycin sulfate across organisms

SEED Subsystems

Subsystem #Specific
DNA-replication 2
D-gluconate and ketogluconates metabolism 1
DNA Repair Base Excision 1
DNA repair, bacterial 1
Fermentations: Mixed acid 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerol fermenation to 1,3-propanediol 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Peptidoglycan Biosynthesis 1
Periplasmic disulfide interchange 1
RNA processing and degradation, bacterial 1
Type IV pilus 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetaldehyde biosynthesis I 1 1 1
L-asparagine biosynthesis II 1 1 1
siroheme biosynthesis 4 4 2
ethanol degradation I 2 2 1
superpathway of L-asparagine biosynthesis 2 2 1
arsenate detoxification III 2 2 1
pyruvate fermentation to ethanol II 2 1 1
pyruvate fermentation to ethanol III 3 3 1
pyruvate fermentation to ethanol I 3 3 1
glycerol degradation I 3 3 1
ethanol degradation II 3 3 1
L-leucine degradation III 3 2 1
L-valine degradation II 3 2 1
acrylate degradation II 3 2 1
L-isoleucine degradation II 3 2 1
L-methionine degradation III 3 1 1
factor 430 biosynthesis 7 3 2
glycerol and glycerophosphodiester degradation 4 4 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 4 1
phytol degradation 4 3 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 6 3
L-phenylalanine degradation III 4 2 1
salidroside biosynthesis 4 2 1
L-tyrosine degradation III 4 2 1
cytidine-5'-diphosphate-glycerol biosynthesis 4 1 1
ethanolamine utilization 5 5 1
acetylene degradation (anaerobic) 5 4 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
(S)-propane-1,2-diol degradation 5 3 1
phenylethanol biosynthesis 5 2 1
arsenic detoxification (plants) 6 5 1
noradrenaline and adrenaline degradation 13 4 2
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 2 2
3-methylbutanol biosynthesis (engineered) 7 6 1
serotonin degradation 7 3 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 3 2
butanol and isobutanol biosynthesis (engineered) 8 2 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 2
peptidoglycan biosynthesis II (staphylococci) 17 12 2
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 2
superpathway of fermentation (Chlamydomonas reinhardtii) 9 8 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 12 1
arsenic detoxification (yeast) 12 4 1
3-hydroxypropanoate cycle 13 7 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 7 1
glyoxylate assimilation 13 5 1
L-tryptophan degradation V (side chain pathway) 13 1 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
mixed acid fermentation 16 16 1
adenosylcobalamin biosynthesis II (aerobic) 33 17 2
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 9 1
arsenic detoxification (mammals) 17 8 1
heterolactic fermentation 18 16 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 1
adenosylcobalamin biosynthesis I (anaerobic) 36 16 2
superpathway of the 3-hydroxypropanoate cycle 18 7 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 1
superpathway of anaerobic sucrose degradation 19 17 1
superpathway of N-acetylneuraminate degradation 22 22 1