Experiment set58S336 for Escherichia coli BW25113

SIR2-HerA

Group: sir2-hera
Media: LB + Kanamycin sulfate (0.05 mg/mL) + Chloramphenicol (34 0.034)
Culturing: Keio_ML9a, bioassay_dish, Aerobic, at 37 (C), (Solid)
By: Kate Miller/Brady Cress/Ben Adler on 10-Apr-23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 8 genes in this experiment

For sir2-hera Kanamycin sulfate in Escherichia coli BW25113

For sir2-hera Kanamycin sulfate across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	2
DNA Repair Base Excision	1
DNA repair, bacterial	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerol fermenation to 1,3-propanediol	1
Peptidoglycan Biosynthesis	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Fructose and mannose metabolism
• Lysine degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
siroheme biosynthesis	4	4	2
arsenate detoxification III	2	2	1
glycerol degradation I	3	3	1
factor 430 biosynthesis	7	3	2
glycerol and glycerophosphodiester degradation	4	4	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	6	3
arsenic detoxification (plants)	6	5	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
arsenic detoxification (yeast)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
arsenic detoxification (mammals)	17	8	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2