Experiment set58S323 for Escherichia coli BW25113

GFP_control

Group: gfp_control
Media: LB + Kanamycin sulfate (0.05 mg/mL) + Chloramphenicol (34 0.034)
Culturing: Keio_ML9a, bioassay_dish, Aerobic, at 37 (C), (Solid)
By: Kate Miller/Brady Cress/Ben Adler on 10-Apr-23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 9 genes in this experiment

For gfp_control Kanamycin sulfate in Escherichia coli BW25113

For gfp_control Kanamycin sulfate across organisms

SEED Subsystems

Subsystem	#Specific
D-gluconate and ketogluconates metabolism	1
DNA-replication	1
DNA repair, bacterial	1
Formate hydrogenase	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerol fermenation to 1,3-propanediol	1
Nitrosative stress	1
Peptidoglycan Biosynthesis	1
Pyrimidine utilization	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Carotenoid biosynthesis - General
• Peptidoglycan biosynthesis
• Ubiquinone and menaquinone biosynthesis
• gamma-Hexachlorocyclohexane degradation
• Tryptophan metabolism
• Sphingolipid metabolism
• Naphthalene and anthracene degradation
• 1,4-Dichlorobenzene degradation
• Limonene and pinene degradation
• Brassinosteroid biosynthesis
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Alkaloid biosynthesis I
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of plant hormones
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Biosynthesis of steroids
• Puromycin biosynthesis
• Lysine degradation
• Histidine metabolism
• Phenylalanine metabolism
• Bisphenol A degradation
• Benzoxazinone biosynthesis
• Cyanoamino acid metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• alpha-Linolenic acid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Toluene and xylene degradation
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Trinitrotoluene degradation
• Styrene degradation
• Methane metabolism
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Zeatin biosynthesis
• Anthocyanin biosynthesis
• Isoflavonoid biosynthesis
• Flavone and flavonol biosynthesis
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
hydrogen production III	1	1	1
hydrogen production V	1	1	1
hydrogen production VIII	1	1	1
arsenate detoxification III	2	2	1
hydrogen production VI	2	1	1
superpathway of hydrogen production	2	1	1
glycerol degradation I	3	3	1
L-carnitine degradation II	3	2	1
glycerol and glycerophosphodiester degradation	4	4	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	6	3
uracil degradation III	5	5	1
arsenic detoxification (plants)	6	5	1
superpathway of photosynthetic hydrogen production	6	3	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
superpathway of fermentation (Chlamydomonas reinhardtii)	9	8	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
L-glutamate degradation VII (to butanoate)	12	5	1
arsenic detoxification (yeast)	12	4	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	10	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
mixed acid fermentation	16	16	1
arsenic detoxification (mammals)	17	8	1