Experiment set57IT026 for Escherichia coli BW25113

Compare to:

Ying_OrganicAcid7 rep B; time point 2

200 most detrimental genes:

  gene name fitness t score description  
b2741 rpoS +4.4 36.5 RNA polymerase sigma factor (NCBI) compare
b2903 gcvP +3.7 32.5 glycine dehydrogenase (NCBI) compare
b2904 gcvH +3.7 24.3 glycine cleavage system protein H (NCBI) compare
b2808 gcvA +3.3 20.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2905 gcvT +3.2 19.7 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2951 yggS +2.5 16.8 predicted enzyme (NCBI) compare
b1095 fabF +2.2 18.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1190 dadX +1.9 10.2 alanine racemase (NCBI) compare
b3164 pnp +1.8 7.4 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1085 b1085 +1.7 4.8 orf, hypothetical protein (VIMSS) compare
b1422 ydcI +1.6 9.7 putative transcriptional regulator LYSR-type (VIMSS) compare
b1831 proQ +1.6 5.0 putative solute/DNA competence effector (NCBI) compare
b2688 gshA +1.6 11.2 glutamate--cysteine ligase (NCBI) compare
b0059 hepA +1.5 14.0 ATP-dependent helicase HepA (NCBI) compare
b3341 rpsG +1.5 2.2 30S ribosomal protein S7 (NCBI) compare
b4203 rplI +1.4 6.3 50S ribosomal protein L9 (NCBI) compare
b2947 gshB +1.3 8.7 glutathione synthetase (NCBI) compare
b2829 ptsP +1.3 11.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1429 tehA +1.3 2.7 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b0882 clpA +1.2 10.5 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b4172 hfq +1.2 1.8 RNA-binding protein Hfq (NCBI) compare
b4071 nrfB +1.1 1.9 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1236 galU +1.1 6.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1294 sapA +1.1 6.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1217 chaB +1.1 2.5 cation transport regulator (NCBI) compare
b2937 speB +1.1 6.7 agmatinase (NCBI) compare
b4000 hupA +1.0 6.0 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1855 lpxM +1.0 5.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1191 b1191 +1.0 6.3 orf, hypothetical protein (VIMSS) compare
b0232 yafN +1.0 0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1583 ynfB +1.0 3.1 hypothetical protein (NCBI) compare
b3842 rfaH +1.0 5.9 transcriptional activator RfaH (NCBI) compare
b1438 ydcQ +1.0 3.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1299 puuR +0.9 3.6 DNA-binding transcriptional repressor (NCBI) compare
b4030 yjbA +0.9 5.6 phosphate-starvation-inducible protein PsiE (NCBI) compare
b3929 rraA +0.9 6.9 ribonuclease activity regulator protein RraA (NCBI) compare
b0955 ycbZ +0.9 6.1 predicted peptidase (NCBI) compare
b1725 yniA +0.9 2.4 predicted phosphotransferase/kinase (NCBI) compare
b0082 mraW +0.9 3.3 S-adenosyl-methyltransferase (NCBI) compare
b3977 tyrU +0.9 1.5 tRNA-Tyr (NCBI) compare
b1824 yobF +0.8 2.6 hypothetical protein (NCBI) compare
b3753 rbsR +0.8 7.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2376 ypdI +0.8 3.6 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b4171 miaA +0.8 3.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0960 b0960 +0.8 6.5 orf, hypothetical protein (VIMSS) compare
b4438 cyaR +0.8 2.3 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1333 uspE +0.8 4.9 stress-induced protein (NCBI) compare
b3778 rep +0.8 5.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3752 rbsK +0.8 6.2 ribokinase (NCBI) compare
b4066 yjcF +0.8 0.8 hypothetical protein (NCBI) compare
b3454 livF +0.7 2.6 ATP-binding component of leucine transport (VIMSS) compare
b0503 ybbB +0.7 1.7 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b0685 ybfE +0.7 3.9 orf, hypothetical protein (VIMSS) compare
b3181 greA +0.7 5.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0610 rnk +0.7 2.8 nucleoside diphosphate kinase regulator (NCBI) compare
b1171 b1171 +0.7 2.8 orf, hypothetical protein (VIMSS) compare
b3632 rfaQ +0.7 6.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2199 ccmC +0.7 1.8 heme exporter subunit (NCBI) compare
b3780 rhlB +0.7 5.7 ATP-dependent RNA helicase (NCBI) compare
b4293 fecI +0.7 2.2 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b4598 yncL +0.7 2.3 hypothetical protein (RefSeq) compare
b3644 yicC +0.7 4.5 hypothetical protein (NCBI) compare
b1222 narX +0.7 3.7 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b3630 rfaP +0.7 5.7 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2171 yeiP +0.7 4.4 putative elongation factor (VIMSS) compare
b1279 yciS +0.7 1.1 conserved inner membrane protein (NCBI) compare
b2579 yfiD +0.6 1.5 pyruvate formate lyase subunit (NCBI) compare
b1090 plsX +0.6 3.7 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2229 b2229 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b0852 rimK +0.6 2.7 ribosomal protein S6 modification protein (NCBI) compare
b4445 omrB +0.6 2.9 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b4450 arcZ +0.6 3.0 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3138 agaB +0.6 2.6 N-acetylgalactosamine-specific enzyme IIB component of PTS (NCBI) compare
b3928 yiiU +0.6 3.4 hypothetical protein (NCBI) compare
b2123 yehR +0.6 1.0 orf, hypothetical protein (VIMSS) compare
b1822 rrmA +0.6 1.6 23S rRNA m1G745 methyltransferase (NCBI) compare
b4651 yibW +0.6 0.6 no description compare
b1273 yciN +0.6 1.5 hypothetical protein (NCBI) compare
b0605 ahpC +0.6 3.4 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2521 sseA +0.6 4.0 putative thiosulfate sulfurtransferase (VIMSS) compare
b1651 gloA +0.6 2.7 glyoxalase I, Ni-dependent (NCBI) compare
b4097 phnK +0.6 1.7 carbon-phosphorus lyase complex subunit (NCBI) compare
b0381 ddlA +0.6 3.8 D-alanylalanine synthetase (NCBI) compare
b3146 yraL +0.6 3.8 predicted methyltransferase (NCBI) compare
b2012 yeeD +0.6 2.0 hypothetical protein (NCBI) compare
b3457 livH +0.6 4.3 leucine/isoleucine/valine transporter subunit (NCBI) compare
b3194 yrbE +0.6 3.4 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1634 tppB +0.6 3.3 putative tripeptide transporter permease (NCBI) compare
b1823 cspC +0.6 1.2 stress protein, member of the CspA-family (NCBI) compare
b3706 trmE +0.6 2.5 tRNA modification GTPase (NCBI) compare
b1916 sdiA +0.6 3.6 DNA-binding transcriptional activator (NCBI) compare
b3523 yhjE +0.6 4.4 predicted transporter (NCBI) compare
b0630 lipB +0.6 1.2 protein of lipoate biosynthesis (VIMSS) compare
b0364 yaiS +0.6 3.1 orf, hypothetical protein (VIMSS) compare
b4570 lomR +0.6 1.8 no description compare
b3670 ilvN +0.6 3.1 acetolactate synthase small subunit (NCBI) compare
b3672 ivbL +0.5 0.7 ilvB operon leader peptide (NCBI) compare
b4461 yfjD +0.5 4.3 predicted inner membrane protein (RefSeq) compare
b1731 cedA +0.5 1.9 cell division modulator (RefSeq) compare
b1189 dadA +0.5 3.7 D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) compare
b2830 nudH +0.5 1.1 dinucleoside polyphosphate hydrolase (NCBI) compare
b2006 yeeW +0.5 2.4 CP4-44 prophage; predicted protein (RefSeq) compare
b3740 gidB +0.5 4.3 glucose-inhibited division protein B (NCBI) compare
b1350 recE +0.5 2.8 Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI) compare
b4260 pepA +0.5 4.1 leucyl aminopeptidase (NCBI) compare
b4701 sokX +0.5 0.6 sok-related sRNA, function unknown (RefSeq) compare
b2576 srmB +0.5 2.4 ATP-dependent RNA helicase (NCBI) compare
b4391 yjjK +0.5 3.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2910 zapA +0.5 3.3 protein that localizes to the cytokinetic ring (NCBI) compare
b1070 flgN +0.5 1.1 export chaperone for FlgK and FlgL (NCBI) compare
b4675 yoaJ +0.5 1.7 expressed protein (RefSeq) compare
b0550 rusA +0.5 1.7 DLP12 prophage; endonuclease RUS (NCBI) compare
b2538 hcaE +0.5 2.0 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI) compare
b2165 yeiN +0.5 1.8 hypothetical protein (NCBI) compare
b3468 yhhN +0.5 3.8 conserved inner membrane protein (NCBI) compare
b4255 yjgD +0.5 2.3 hypothetical protein (NCBI) compare
b3911 cpxA +0.5 3.9 two-component sensor protein (NCBI) compare
b1540 ydfH +0.5 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3206 npr +0.5 3.1 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b3346 yheO +0.5 4.1 orf, hypothetical protein (VIMSS) compare
b3102 yqjG +0.5 1.5 predicted S-transferase (NCBI) compare
b1040 csgD +0.5 1.3 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b4174 hflK +0.5 3.7 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2954 yggV +0.5 0.9 putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI) compare
b2474 ypfI +0.5 3.3 predicted hydrolase (NCBI) compare
b0968 yccX +0.5 2.1 predicted acylphosphatase (NCBI) compare
b2889 idi +0.5 2.6 isopentenyl-diphosphate delta-isomerase (NCBI) compare
b1255 yciC +0.5 1.5 hypothetical protein (NCBI) compare
b2303 folX +0.5 3.2 D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI) compare
b3616 tdh +0.5 2.9 L-threonine 3-dehydrogenase (NCBI) compare
b2944 sprT +0.5 1.1 hypothetical protein (NCBI) compare
b4435 isrC +0.5 1.5 unknown RNA (NCBI) compare
b1379 hslJ +0.5 3.0 heat-inducible protein (NCBI) compare
b2114 metG +0.5 1.5 methionyl-tRNA synthetase (NCBI) compare
b1640 ydhH +0.5 2.6 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1952 dsrB +0.5 2.1 hypothetical protein (NCBI) compare
b1627 rsxA +0.5 2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2742 nlpD +0.5 3.1 predicted outer membrane lipoprotein (NCBI) compare
b4135 yjdC +0.5 3.6 orf, hypothetical protein (VIMSS) compare
b4110 yjcZ +0.5 3.4 orf, hypothetical protein (VIMSS) compare
b4522 yciX_1 +0.5 0.9 hypothetical protein (NCBI) compare
b3631 rfaG +0.5 3.9 glucosyltransferase I (NCBI) compare
b2643 yfjX +0.5 0.8 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b0178 hlpA +0.5 2.0 periplasmic chaperone (NCBI) compare
b1606 folM +0.5 3.0 short chain dehydrogenase (NCBI) compare
b2421 cysM +0.4 1.8 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1593 b1593 +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b3095 yqjA +0.4 3.3 conserved inner membrane protein (NCBI) compare
b0627 tatE +0.4 1.7 twin arginine translocase protein E (NCBI) compare
b3455 livG +0.4 2.6 leucine/isoleucine/valine transporter subunit (NCBI) compare
b1329 b1329 +0.4 3.3 putative transport periplasmic protein (VIMSS) compare
b4515 ybgT +0.4 1.1 hypothetical protein (NCBI) compare
b0998 torD +0.4 0.9 chaperone protein TorD (NCBI) compare
b3999 yjaG +0.4 2.0 hypothetical protein (NCBI) compare
b1341 b1341 +0.4 3.1 orf, hypothetical protein (VIMSS) compare
b4175 hflC +0.4 3.2 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0382 yaiB +0.4 2.9 hypothetical protein (NCBI) compare
b2506 yfgI +0.4 1.4 hypothetical protein (NCBI) compare
b0778 bioD +0.4 1.9 dethiobiotin synthetase (NCBI) compare
b0851 nfsA +0.4 0.7 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1743 spy +0.4 0.8 envelope stress induced periplasmic protein (NCBI) compare
b3741 gidA +0.4 2.1 glucose-inhibited division protein A (NCBI) compare
b3867 hemN +0.4 3.5 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b1390 paaC +0.4 2.2 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b1950 fliR +0.4 1.5 flagellar biosynthesis protein R (NCBI) compare
b3229 sspA +0.4 1.6 stringent starvation protein A (NCBI) compare
b1753 ynjA +0.4 1.2 hypothetical protein (NCBI) compare
b0488 ybbJ +0.4 2.3 conserved inner membrane protein (RefSeq) compare
b1187 fadR +0.4 2.3 fatty acid metabolism regulator (NCBI) compare
b2644 yfjY +0.4 0.9 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b0831 yliC +0.4 2.2 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1073 flgB +0.4 2.5 flagellar basal-body rod protein B (NCBI) compare
b2052 fcl +0.4 2.6 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b3484 yhhI +0.4 1.1 predicted transposase (NCBI) compare
b4504 ykfH +0.4 2.4 hypothetical protein (NCBI) compare
b1041 csgB +0.4 2.1 curlin nucleator protein, minor subunit in curli complex (NCBI) compare
b1874 cutC +0.4 1.7 copper homeostasis protein (NCBI) compare
b0238 gpt +0.4 1.6 xanthine phosphoribosyltransferase (NCBI) compare
b4041 plsB +0.4 1.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b3934 cytR +0.4 3.3 DNA-binding transcriptional dual regulator (NCBI) compare
b2665 ygaU +0.4 1.2 hypothetical protein (NCBI) compare
b4373 rimI +0.4 1.2 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1141 ymfG +0.4 1.2 e14 prophage; predicted excisionase (NCBI) compare
b0783 moaC +0.4 2.1 molybdenum cofactor biosynthesis protein C (NCBI) compare
b0881 clpS +0.4 1.1 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b2637 yfjT +0.4 1.3 CP4-57 prophage; predicted protein (NCBI) compare
b3460 livJ +0.4 3.0 leucine/isoleucine/valine transporter subunit (NCBI) compare
b0240 crl +0.4 2.6 DNA-binding transcriptional regulator (NCBI) compare
b0389 yaiA +0.4 1.3 hypothetical protein (NCBI) compare
b1515 lsrD +0.4 1.6 AI2 transporter (NCBI) compare
b1462 b1462 +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1920 fliY +0.4 1.0 cystine transporter subunit (NCBI) compare
b1604 ydgH +0.4 1.4 hypothetical protein (NCBI) compare
b1335 ogt +0.4 2.1 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b1842 holE +0.4 0.7 DNA polymerase III, theta subunit (NCBI) compare
b2572 rseA +0.4 1.5 anti-sigma factor (NCBI) compare
b2175 spr +0.4 1.7 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0658 ybeX +0.4 1.0 predicteed ion transport (NCBI) compare
b2919 ygfG +0.4 1.6 putative enzyme (VIMSS) compare
b1110 ycfJ +0.4 2.1 hypothetical protein (NCBI) compare


Specific Phenotypes

For 8 genes in this experiment

For carbon source Ying_OrganicAcid7 in Escherichia coli BW25113

For carbon source Ying_OrganicAcid7 across organisms