Experiment set57IT008 for Escherichia coli BW25113
Ying_OrganicAcid7 rep B; time point 1
Group: carbon sourceMedia: SDM_noCarbon + 1X Ying_OrganicAcid7
Culturing: Keio_ML9a, tube, Aerobic, at 28 (C), shaken=180 rpm
By: Ying on 19-Oct-22
Media components: 1.5 g/L Ammonium chloride, 0.6 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Ying_OrganicAcid7 1x includes: 366 uM Sodium L-Lactate, 366 uM D,L-Malic Acid, 366 uM Citric Acid, 366 uM Succinic Acid, 366 uM Sodium pyruvate, 366 uM a-Ketoglutaric acid disodium salt hydrate, 366 uM L-Citrulline
Specific Phenotypes
For 3 genes in this experiment
For carbon source Ying_OrganicAcid7 in Escherichia coli BW25113
For carbon source Ying_OrganicAcid7 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycolysis and Gluconeogenesis | 1 |
| Glycolysis and Gluconeogenesis, including Archaeal enzymes | 1 |
| Pyruvate metabolism I: anaplerotic reactions, PEP | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: