Experiment set56IT083 for Escherichia coli BW25113

Compare to:

LB Anaerobic Allura Red AC 250 uM

200 most detrimental genes:

  gene name fitness t score description  
b2217 rcsB +1.5 11.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3512 GadE +1.4 4.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1493 gadB +1.4 2.2 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b2096 gatY +1.3 8.4 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2156 lysP +1.2 9.9 lysine transporter (NCBI) compare
b2289 lrhA +1.1 8.5 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b4402 yjjY +1.1 2.2 hypothetical protein (NCBI) compare
b3375 yhfR +1.1 7.4 putative transcriptional regulator (VIMSS) compare
b4676 yoaK +1.0 1.2 expressed protein (RefSeq) compare
b3779 gpp +1.0 9.0 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b0955 ycbZ +1.0 7.3 predicted peptidase (NCBI) compare
b1492 gadC +1.0 5.9 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b2189 proL +1.0 3.2 tRNA-Pro (NCBI) compare
b2236 yfaE +0.9 2.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2659 b2659 +0.9 2.9 orf, hypothetical protein (VIMSS) compare
b0092 ddlB +0.9 5.1 D-alanylalanine synthetase (NCBI) compare
b3495 uspA +0.9 7.5 universal stress global response regulator (NCBI) compare
b1159 mcrA +0.9 1.5 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2285 nuoE +0.8 2.2 NADH dehydrogenase subunit E (NCBI) compare
b1244 oppB +0.8 6.2 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2093 gatB +0.8 3.3 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b3745 yieM +0.8 7.6 predicted von Willibrand factor containing protein (NCBI) compare
b2287 nuoB +0.8 2.9 NADH dehydrogenase subunit B (NCBI) compare
b3746 yieN +0.8 7.3 putative 2-component regulator (VIMSS) compare
b2095 gatZ +0.8 4.2 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b0435 bolA +0.8 5.4 possible regulator of murein genes (VIMSS) compare
b2502 ppx +0.8 5.3 exopolyphosphatase (NCBI) compare
b2531 iscR +0.8 2.6 DNA-binding transcriptional repressor (NCBI) compare
b1247 oppF +0.8 5.8 oligopeptide transporter subunit (NCBI) compare
b2946 yggJ +0.7 3.7 orf, hypothetical protein (VIMSS) compare
b2911 ssrS +0.7 1.8 6S regulatory RNA (NCBI) compare
b2916 argP +0.7 5.1 chromosome replication initiation inhibitor protein (NCBI) compare
b0191 yaeJ +0.7 4.2 hypothetical protein (NCBI) compare
b1243 oppA +0.7 4.8 oligopeptide transporter subunit (NCBI) compare
b3816 corA +0.7 5.4 magnesium/nickel/cobalt transporter (NCBI) compare
b0564 appY +0.7 1.0 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b4133 cadC +0.7 6.2 DNA-binding transcriptional activator (NCBI) compare
b4232 fbp +0.7 5.5 fructose-1,6-bisphosphatase (NCBI) compare
b2743 pcm +0.7 3.7 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2280 nuoJ +0.6 3.6 NADH dehydrogenase subunit J (NCBI) compare
b2277 nuoM +0.6 4.2 NADH dehydrogenase subunit M (NCBI) compare
b1246 oppD +0.6 5.4 oligopeptide transporter ATP-binding component (NCBI) compare
b1476 fdnI +0.6 1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1827 kdgR +0.6 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0104 guaC +0.6 5.1 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b4503 yafF +0.6 0.9 no description compare
b1778 yeaA +0.6 1.5 methionine sulfoxide reductase B (NCBI) compare
b4699 fnrS +0.6 0.8 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3181 greA +0.6 2.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1658 purR +0.6 3.6 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1793 yoaF +0.6 1.8 conserved outer membrane protein (NCBI) compare
b1245 oppC +0.6 4.6 oligopeptide transporter subunit (NCBI) compare
b2814 metZ +0.6 1.6 tRNA-Met (NCBI) compare
b2894 xerD +0.6 0.5 site-specific tyrosine recombinase XerD (NCBI) compare
b4596 yciZ +0.6 2.0 hypothetical protein (RefSeq) compare
b1465 narV +0.6 1.2 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b3870 glnA +0.6 2.0 glutamine synthetase (NCBI) compare
b4515 ybgT +0.6 1.5 hypothetical protein (NCBI) compare
b4603 rseX +0.6 0.8 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3146 yraL +0.6 3.7 predicted methyltransferase (NCBI) compare
b3166 truB +0.6 4.0 tRNA pseudouridine synthase B (NCBI) compare
b2809 b2809 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b2281 nuoI +0.5 1.9 NADH dehydrogenase subunit I (NCBI) compare
b3107 yhaL +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b3193 yrbD +0.5 4.5 predicted ABC-type organic solvent transporter (NCBI) compare
b4041 plsB +0.5 1.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b3341 rpsG +0.5 1.1 30S ribosomal protein S7 (NCBI) compare
b2283 nuoG +0.5 3.8 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2284 nuoF +0.5 2.9 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1284 yciT +0.5 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2433 b2433 +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b4438 cyaR +0.5 2.2 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b0979 appB +0.5 3.0 cytochrome bd-II oxidase, subunit II (NCBI) compare
b2047 wcaJ +0.5 3.3 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b2642 yfjW +0.5 0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4313 fimE +0.5 3.9 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b0692 potE +0.5 3.3 putrescine/proton symporter: putrescine/ornithine antiporter (NCBI) compare
b2082 ogrK +0.5 1.5 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b0998 torD +0.5 1.1 chaperone protein TorD (NCBI) compare
b0014 dnaK +0.5 0.9 molecular chaperone DnaK (NCBI) compare
b2903 gcvP +0.5 4.3 glycine dehydrogenase (NCBI) compare
b1187 fadR +0.5 2.7 fatty acid metabolism regulator (NCBI) compare
b1642 slyA +0.5 1.7 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1307 pspD +0.5 1.6 peripheral inner membrane phage-shock protein (NCBI) compare
b0310 ykgH +0.5 1.0 predicted inner membrane protein (NCBI) compare
b4126 yjdI +0.5 4.0 hypothetical protein (NCBI) compare
b2637 yfjT +0.5 1.9 CP4-57 prophage; predicted protein (NCBI) compare
b2363 yfdT +0.5 1.8 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b0828 iaaA +0.5 1.9 L-asparaginase (NCBI) compare
b4062 soxS +0.5 2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b3516 gadX +0.5 4.1 DNA-binding transcriptional dual regulator (NCBI) compare
b4222 ytfP +0.5 3.0 hypothetical protein (NCBI) compare
b4451 ryhB +0.5 1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2282 nuoH +0.5 1.7 NADH dehydrogenase subunit H (NCBI) compare
b4511 ybdZ +0.5 1.6 hypothetical protein (NCBI) compare
b2291 yfbR +0.5 1.1 hypothetical protein (NCBI) compare
b1685 ydiH +0.5 1.2 predicted protein (RefSeq) compare
b3206 npr +0.5 3.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2570 rseC +0.5 2.4 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0617 citD +0.5 1.5 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b4363 yjjB +0.5 2.5 conserved inner membrane protein (RefSeq) compare
b1765 ydjA +0.5 0.8 predicted oxidoreductase (NCBI) compare
b3068 ygjF +0.5 2.1 G/U mismatch-specific DNA glycosylase (NCBI) compare
b0535 fimZ +0.5 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3194 yrbE +0.5 3.7 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4527 b1354 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b0965 b0965 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1615 uidC +0.4 2.5 membrane-associated protein (VIMSS) compare
b1536 ydeI +0.4 0.7 hypothetical protein (NCBI) compare
b2092 gatC +0.4 1.9 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b1829 htpX +0.4 2.8 heat shock protein HtpX (NCBI) compare
b0487 cueR +0.4 2.6 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b4541 yehK +0.4 2.0 hypothetical protein (NCBI) compare
b1709 btuD +0.4 0.5 vitamin B12-transporter ATPase (NCBI) compare
b0001 thrL +0.4 1.9 thr operon leader peptide (NCBI) compare
b0990 cspG +0.4 0.6 DNA-binding transcriptional regulator (NCBI) compare
b2421 cysM +0.4 2.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b2741 rpoS +0.4 2.5 RNA polymerase sigma factor (NCBI) compare
b2666 yqaE +0.4 1.8 predicted membrane protein (NCBI) compare
b2094 gatA +0.4 3.3 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b2471 yffB +0.4 1.8 hypothetical protein (NCBI) compare
b2195 ccmG +0.4 1.8 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b4406 yaeP +0.4 1.6 hypothetical protein (NCBI) compare
b2278 nuoL +0.4 2.8 NADH dehydrogenase subunit L (NCBI) compare
b2362 yfdS +0.4 2.0 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2424 cysU +0.4 1.2 sulfate/thiosulfate transporter subunit (NCBI) compare
b2279 nuoK +0.4 0.7 NADH dehydrogenase subunit K (NCBI) compare
b3191 yrbB +0.4 2.9 orf, hypothetical protein (VIMSS) compare
b1258 yciF +0.4 0.5 hypothetical protein (NCBI) compare
b4453 ldrD +0.4 1.1 toxic polypeptide, small (NCBI) compare
b1259 yciG +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1348 lar +0.4 1.1 Rac prophage; restriction alleviation protein (NCBI) compare
b2904 gcvH +0.4 1.5 glycine cleavage system protein H (NCBI) compare
b4571 wbbL +0.4 1.7 no description compare
b0746 valZ +0.4 1.1 tRNA-Val (NCBI) compare
b2203 napB +0.4 2.0 cytochrome c-type protein (VIMSS) compare
b2080 b2080 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b2646 ypjF +0.4 1.7 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b4011 yjaA +0.4 1.6 hypothetical protein (NCBI) compare
b1333 uspE +0.4 2.2 stress-induced protein (NCBI) compare
b2539 hcaF +0.4 2.0 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) compare
b1583 ynfB +0.4 0.8 hypothetical protein (NCBI) compare
b4151 frdD +0.4 2.1 fumarate reductase subunit D (NCBI) compare
b1289 ycjD +0.4 1.9 hypothetical protein (NCBI) compare
b3507 dctR +0.4 3.3 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b0636 ybeA +0.4 2.1 hypothetical protein (NCBI) compare
b4089 rpiR +0.4 3.2 transcriptional repressor of rpiB expression (VIMSS) compare
b1155 ymfS +0.4 2.0 e14 prophage; predicted protein (NCBI) compare
b1823 cspC +0.4 1.1 stress protein, member of the CspA-family (NCBI) compare
b1394 paaG +0.4 1.6 enoyl-CoA hydratase (NCBI) compare
b1025 ycdT +0.4 0.8 predicted diguanylate cyclase (NCBI) compare
b4497 yeeL +0.4 0.8 no description compare
b2426 ucpA +0.4 1.3 putative oxidoreductase (VIMSS) compare
b4045 yjbJ +0.4 0.7 predicted stress response protein (NCBI) compare
b3119 tdcR +0.4 1.4 threonine dehydratase operon activator protein (VIMSS) compare
b1008 ycdI +0.4 2.2 hypothetical protein (NCBI) compare
b3390 aroK +0.4 0.9 shikimate kinase I (NCBI) compare
b0082 mraW +0.4 1.3 S-adenosyl-methyltransferase (NCBI) compare
b1446 ydcY +0.4 1.2 hypothetical protein (NCBI) compare
b3195 yrbF +0.4 3.1 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2114 metG +0.4 1.1 methionyl-tRNA synthetase (NCBI) compare
b0018 mokC +0.4 0.8 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4369 leuP +0.4 0.8 tRNA-Leu (NCBI) compare
b4214 cysQ +0.4 3.1 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b3889 yiiE +0.4 1.2 predicted transcriptional regulator (RefSeq) compare
b1233 ychJ +0.4 1.7 hypothetical protein (NCBI) compare
b1689 b1689 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1946 fliN +0.4 1.2 flagellar motor switch protein (NCBI) compare
b2988 gss +0.4 2.9 fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI) compare
b0977 hyaF +0.4 1.7 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b1562 hokD +0.3 1.4 Qin prophage; small toxic polypeptide (NCBI) compare
b1189 dadA +0.3 2.1 D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) compare
b1317 ycjU +0.3 1.5 predicted beta-phosphoglucomutase (NCBI) compare
b3892 fdoI +0.3 2.8 formate dehydrogenase-O, cytochrome b556 subunit (NCBI) compare
b1156 tfaE +0.3 2.2 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b2186 yejK +0.3 3.0 nucleoid-associated protein NdpA (NCBI) compare
b1959 yedA +0.3 2.1 predicted inner membrane protein (NCBI) compare
b2906 visC +0.3 2.0 hypothetical protein (NCBI) compare
b1573 ydfC +0.3 0.6 Qin prophage; predicted protein (RefSeq) compare
b1582 ynfA +0.3 1.4 hypothetical protein (NCBI) compare
b4127 yjdJ +0.3 2.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0164 yaeI +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b0809 glnQ +0.3 2.1 glutamine ABC transporter ATP-binding protein (NCBI) compare
b1731 cedA +0.3 1.6 cell division modulator (RefSeq) compare
b0931 pncB +0.3 1.8 nicotinate phosphoribosyltransferase (NCBI) compare
b2580 ung +0.3 1.9 uracil-DNA glycosylase (NCBI) compare
b1868 yecE +0.3 1.9 hypothetical protein (NCBI) compare
b1961 dcm +0.3 1.0 DNA cytosine methylase (NCBI) compare
b1150 ymfR +0.3 0.6 e14 prophage; predicted protein (NCBI) compare
b2197 ccmE +0.3 1.9 periplasmic heme chaperone (NCBI) compare
b2436 hemF +0.3 1.7 coproporphyrinogen III oxidase (NCBI) compare
b3192 yrbC +0.3 2.7 predicted ABC-type organic solvent transporter (NCBI) compare
b3672 ivbL +0.3 1.5 ilvB operon leader peptide (NCBI) compare
b1785 yeaI +0.3 1.3 predicted diguanylate cyclase (NCBI) compare
b2439 eutL +0.3 2.1 predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI) compare
b4391 yjjK +0.3 2.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1607 ydgC +0.3 0.7 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3378 yhfU +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1941 fliI +0.3 2.3 flagellum-specific ATP synthase (NCBI) compare
b3239 yhcO +0.3 1.4 predicted barnase inhibitor (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Allura Red AC in Escherichia coli BW25113

For stress Allura Red AC across organisms