Experiment set56IT082 for Escherichia coli BW25113

Compare to:

LB Anaerobic Allura Red AC 250 uM

200 most detrimental genes:

  gene name fitness t score description  
b2498 upp +2.3 4.8 uracil phosphoribosyltransferase (VIMSS) compare
b2217 rcsB +1.5 10.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3390 aroK +1.3 3.9 shikimate kinase I (NCBI) compare
b4704 arrS +1.3 0.6 Antisense sRNA ArrS, function unknown (NCBI) compare
b2189 proL +1.3 4.5 tRNA-Pro (NCBI) compare
b2156 lysP +1.2 10.2 lysine transporter (NCBI) compare
b2096 gatY +1.2 7.5 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2289 lrhA +1.2 9.3 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b3375 yhfR +1.0 7.9 putative transcriptional regulator (VIMSS) compare
b2093 gatB +1.0 6.6 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2285 nuoE +1.0 3.9 NADH dehydrogenase subunit E (NCBI) compare
b1492 gadC +1.0 5.8 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b3512 GadE +1.0 3.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1882 cheY +1.0 0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b0014 dnaK +0.9 3.1 molecular chaperone DnaK (NCBI) compare
b0955 ycbZ +0.9 7.5 predicted peptidase (NCBI) compare
b3779 gpp +0.9 8.1 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b0191 yaeJ +0.9 5.1 hypothetical protein (NCBI) compare
b1244 oppB +0.9 6.6 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2095 gatZ +0.9 4.6 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b2916 argP +0.8 5.4 chromosome replication initiation inhibitor protein (NCBI) compare
b3745 yieM +0.8 7.6 predicted von Willibrand factor containing protein (NCBI) compare
b3746 yieN +0.8 7.1 putative 2-component regulator (VIMSS) compare
b3495 uspA +0.8 7.1 universal stress global response regulator (NCBI) compare
b3816 corA +0.8 6.4 magnesium/nickel/cobalt transporter (NCBI) compare
b0357 yaiN +0.8 2.7 putative alpha helix chain (VIMSS) compare
b2236 yfaE +0.7 3.5 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3181 greA +0.7 3.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4699 fnrS +0.7 1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2911 ssrS +0.7 2.0 6S regulatory RNA (NCBI) compare
b2502 ppx +0.7 6.2 exopolyphosphatase (NCBI) compare
b2282 nuoH +0.7 2.8 NADH dehydrogenase subunit H (NCBI) compare
b1243 oppA +0.7 4.6 oligopeptide transporter subunit (NCBI) compare
b1259 yciG +0.7 2.3 orf, hypothetical protein (VIMSS) compare
b1247 oppF +0.7 4.8 oligopeptide transporter subunit (NCBI) compare
b2904 gcvH +0.7 2.4 glycine cleavage system protein H (NCBI) compare
b0990 cspG +0.7 0.6 DNA-binding transcriptional regulator (NCBI) compare
b4232 fbp +0.7 5.3 fructose-1,6-bisphosphatase (NCBI) compare
b4133 cadC +0.7 6.0 DNA-binding transcriptional activator (NCBI) compare
b1727 yniC +0.7 2.4 predicted hydrolase (NCBI) compare
b2946 yggJ +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b1187 fadR +0.7 3.5 fatty acid metabolism regulator (NCBI) compare
b1735 chbR +0.6 3.0 DNA-binding transcriptional dual regulator (NCBI) compare
b0104 guaC +0.6 5.1 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b2743 pcm +0.6 2.7 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b1245 oppC +0.6 4.9 oligopeptide transporter subunit (NCBI) compare
b1493 gadB +0.6 0.9 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b0092 ddlB +0.6 3.0 D-alanylalanine synthetase (NCBI) compare
b1874 cutC +0.6 0.9 copper homeostasis protein (NCBI) compare
b3516 gadX +0.6 5.2 DNA-binding transcriptional dual regulator (NCBI) compare
b2080 b2080 +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b0881 clpS +0.6 2.1 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b0247 ykfG +0.6 1.3 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2642 yfjW +0.6 0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3206 npr +0.6 3.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4598 yncL +0.6 2.1 hypothetical protein (RefSeq) compare
b2421 cysM +0.6 3.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b4126 yjdI +0.6 4.4 hypothetical protein (NCBI) compare
b2277 nuoM +0.5 3.3 NADH dehydrogenase subunit M (NCBI) compare
b2659 b2659 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1556 b1556 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1348 lar +0.5 1.4 Rac prophage; restriction alleviation protein (NCBI) compare
b3166 truB +0.5 3.6 tRNA pseudouridine synthase B (NCBI) compare
b0746 valZ +0.5 1.7 tRNA-Val (NCBI) compare
b2531 iscR +0.5 1.7 DNA-binding transcriptional repressor (NCBI) compare
b2012 yeeD +0.5 1.7 hypothetical protein (NCBI) compare
b4551 yheV +0.5 1.5 hypothetical protein (NCBI) compare
b3146 yraL +0.5 3.5 predicted methyltransferase (NCBI) compare
b2783 chpR +0.5 1.1 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b4408 csrB +0.5 2.5 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1148 ymfM +0.5 2.7 e14 prophage; predicted protein (NCBI) compare
b0435 bolA +0.5 3.0 possible regulator of murein genes (VIMSS) compare
b2903 gcvP +0.5 4.3 glycine dehydrogenase (NCBI) compare
b4222 ytfP +0.5 3.6 hypothetical protein (NCBI) compare
b0730 mngR +0.5 1.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4127 yjdJ +0.5 3.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2811 ygdK +0.5 2.2 predicted Fe-S metabolism protein (NCBI) compare
b2283 nuoG +0.5 3.4 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2280 nuoJ +0.5 2.5 NADH dehydrogenase subunit J (NCBI) compare
b1658 purR +0.5 2.6 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1607 ydgC +0.5 1.1 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3193 yrbD +0.5 3.8 predicted ABC-type organic solvent transporter (NCBI) compare
b4214 cysQ +0.5 3.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b1582 ynfA +0.5 2.0 hypothetical protein (NCBI) compare
b2284 nuoF +0.5 2.7 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1503 ydeR +0.5 2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b2618 b2618 +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1429 tehA +0.5 0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b2504 yfgG +0.5 1.0 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1573 ydfC +0.5 1.1 Qin prophage; predicted protein (RefSeq) compare
b4406 yaeP +0.4 1.9 hypothetical protein (NCBI) compare
b2094 gatA +0.4 3.4 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b4294 insA-7 +0.4 1.9 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b1804 rnd +0.4 1.8 ribonuclease D (NCBI) compare
b1380 ldhA +0.4 2.0 D-lactate dehydrogenase (NCBI) compare
b3382 yhfY +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b1246 oppD +0.4 3.2 oligopeptide transporter ATP-binding component (NCBI) compare
b2092 gatC +0.4 1.9 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b3739 atpI +0.4 1.8 membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS) compare
b4522 yciX_1 +0.4 1.6 hypothetical protein (NCBI) compare
b4116 adiY +0.4 3.8 DNA-binding transcriptional activator (NCBI) compare
b2346 vacJ +0.4 3.0 predicted lipoprotein (NCBI) compare
b4403 yjtD +0.4 2.4 predicted rRNA methyltransferase (NCBI) compare
b4653 yicT +0.4 0.9 no description compare
b0977 hyaF +0.4 2.4 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b4546 ypeB +0.4 0.9 hypothetical protein (NCBI) compare
b1390 paaC +0.4 2.8 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b2023 hisH +0.4 2.7 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3195 yrbF +0.4 3.3 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1976 b1976 +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b1829 htpX +0.4 2.1 heat shock protein HtpX (NCBI) compare
b2674 nrdI +0.4 2.7 hypothetical protein (NCBI) compare
b3207 yrbL +0.4 2.3 hypothetical protein (NCBI) compare
b1186 nhaB +0.4 2.6 NhaB sodium/proton transporter (Katherine Huang) compare
b0294 ykgK +0.4 1.9 predicted regulator (NCBI) compare
b0706 ybfD +0.4 1.4 hypothetical protein (NCBI) compare
b4233 mpl +0.4 3.3 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI) compare
b0692 potE +0.4 2.5 putrescine/proton symporter: putrescine/ornithine antiporter (NCBI) compare
b2276 nuoN +0.4 2.2 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3194 yrbE +0.4 3.0 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0426 yajQ +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b4592 yccB +0.4 1.0 hypothetical protein (RefSeq) compare
b1394 paaG +0.4 2.1 enoyl-CoA hydratase (NCBI) compare
b4631 ykgQ +0.4 1.0 no description compare
b2919 ygfG +0.4 2.7 putative enzyme (VIMSS) compare
b0974 hyaC +0.4 1.5 hydrogenase 1, b-type cytochrome subunit (NCBI) compare
b3191 yrbB +0.4 3.2 orf, hypothetical protein (VIMSS) compare
b4003 zraS +0.4 2.8 sensory histidine kinase in two-component regulatory system with ZraR (NCBI) compare
b2288 nuoA +0.4 1.3 NADH dehydrogenase I chain A (VIMSS) compare
b1557 cspB +0.4 1.2 Qin prophage; cold shock protein (NCBI) compare
b1849 purT +0.4 2.0 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1007 b1007 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1992 cobS +0.4 1.2 cobalamin synthase (NCBI) compare
b2279 nuoK +0.4 0.7 NADH dehydrogenase subunit K (NCBI) compare
b2445 yffN +0.4 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b0710 ybgI +0.4 1.7 conserved metal-binding protein (NCBI) compare
b4418 sraB +0.4 0.6 unknown RNA (NCBI) compare
b1446 ydcY +0.4 1.1 hypothetical protein (NCBI) compare
b2829 ptsP +0.4 3.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3068 ygjF +0.4 2.0 G/U mismatch-specific DNA glycosylase (NCBI) compare
b0866 ybjQ +0.4 0.8 hypothetical protein (NCBI) compare
b1156 tfaE +0.4 2.0 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b1284 yciT +0.4 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b0850 ybjC +0.4 1.2 predicted inner membrane protein (NCBI) compare
b3427 yzgL +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b3810 yigA +0.3 2.8 hypothetical protein (NCBI) compare
b1315 ycjS +0.3 2.3 predicted oxidoreductase, NADH-binding (NCBI) compare
b0494 tesA +0.3 2.0 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b3658 selC +0.3 1.4 tRNA-Sec (NCBI) compare
b1475 fdnH +0.3 2.0 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b0615 citF +0.3 1.9 citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI) compare
b2622 intA +0.3 2.5 CP4-57 prophage; integrase (NCBI) compare
b0636 ybeA +0.3 2.1 hypothetical protein (NCBI) compare
b1518 lsrG +0.3 0.9 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1824 yobF +0.3 1.5 hypothetical protein (NCBI) compare
b2286 nuoC +0.3 2.1 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2291 yfbR +0.3 1.7 hypothetical protein (NCBI) compare
b0082 mraW +0.3 1.3 S-adenosyl-methyltransferase (NCBI) compare
b1742 b1742 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1103 ycfF +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b4089 rpiR +0.3 2.7 transcriptional repressor of rpiB expression (VIMSS) compare
b0502 ylbG +0.3 1.1 no description compare
b1827 kdgR +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b0473 htpG +0.3 2.7 heat shock protein 90 (NCBI) compare
b3507 dctR +0.3 2.9 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b4011 yjaA +0.3 0.9 hypothetical protein (NCBI) compare
b2281 nuoI +0.3 1.2 NADH dehydrogenase subunit I (NCBI) compare
b0013 yaaI +0.3 1.6 hypothetical protein (NCBI) compare
b4513 kdpF +0.3 1.4 potassium ion accessory transporter subunit (NCBI) compare
b0618 citC +0.3 2.3 citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS) compare
b0755 gpmA +0.3 2.1 phosphoglyceromutase (NCBI) compare
b0471 ybaB +0.3 1.0 hypothetical protein (NCBI) compare
b1025 ycdT +0.3 0.6 predicted diguanylate cyclase (NCBI) compare
b2471 yffB +0.3 1.3 hypothetical protein (NCBI) compare
b1642 slyA +0.3 1.0 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3688 yidQ +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b2003 yeeT +0.3 1.3 CP4-44 prophage; predicted protein (NCBI) compare
b1575 dicB +0.3 0.5 Qin prophage; cell division inhibition protein (NCBI) compare
b3192 yrbC +0.3 2.4 predicted ABC-type organic solvent transporter (NCBI) compare
b3397 nudE +0.3 2.4 ADP-ribose diphosphatase (NCBI) compare
b0533 sfmH +0.3 2.1 predicted fimbrial-like adhesin protein (RefSeq) compare
b0375 yaiV +0.3 1.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1242 ychE +0.3 1.5 predicted inner membrane protein (NCBI) compare
b1696 ydiP +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1778 yeaA +0.3 1.0 methionine sulfoxide reductase B (NCBI) compare
b2437 yfeG +0.3 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2278 nuoL +0.3 2.3 NADH dehydrogenase subunit L (NCBI) compare
b3107 yhaL +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1343 dbpA +0.3 1.9 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b3341 rpsG +0.3 0.6 30S ribosomal protein S7 (NCBI) compare
b4391 yjjK +0.3 2.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2814 metZ +0.3 0.8 tRNA-Met (NCBI) compare
b1773 ydjI +0.3 0.9 predicted aldolase (NCBI) compare
b1743 spy +0.3 2.3 envelope stress induced periplasmic protein (NCBI) compare
b1165 ymgA +0.3 0.3 hypothetical protein (NCBI) compare
b2050 wcaI +0.3 1.8 predicted glycosyl transferase (NCBI) compare
b2679 proX +0.3 1.9 glycine betaine transporter subunit (NCBI) compare
b1860 ruvB +0.3 0.2 Holliday junction DNA helicase B (NCBI) compare
b0213 yafS +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b0888 trxB +0.3 1.0 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Allura Red AC in Escherichia coli BW25113

For stress Allura Red AC across organisms