Experiment set56IT081 for Escherichia coli BW25113

Compare to:

LB Anaerobic Sulfasalazine 250 uM

200 most detrimental genes:

  gene name fitness t score description  
b3390 aroK +1.6 5.0 shikimate kinase I (NCBI) compare
b2217 rcsB +1.6 11.4 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2289 lrhA +1.4 9.6 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b0564 appY +1.3 2.3 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b2096 gatY +1.3 9.6 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4699 fnrS +1.2 2.0 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3512 GadE +1.2 4.7 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b3375 yhfR +1.1 8.5 putative transcriptional regulator (VIMSS) compare
b2498 upp +1.1 3.4 uracil phosphoribosyltransferase (VIMSS) compare
b2156 lysP +1.1 9.3 lysine transporter (NCBI) compare
b1291 sapD +1.1 1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0191 yaeJ +1.0 5.4 hypothetical protein (NCBI) compare
b3779 gpp +1.0 8.9 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b1370 insH-5 +1.0 3.4 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2093 gatB +1.0 5.6 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b1765 ydjA +1.0 1.8 predicted oxidoreductase (NCBI) compare
b0955 ycbZ +0.9 7.4 predicted peptidase (NCBI) compare
b1492 gadC +0.9 5.6 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b4402 yjjY +0.9 1.4 hypothetical protein (NCBI) compare
b0232 yafN +0.9 1.0 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0247 ykfG +0.9 2.2 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b3745 yieM +0.8 7.8 predicted von Willibrand factor containing protein (NCBI) compare
b2095 gatZ +0.8 5.7 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b3341 rpsG +0.8 1.8 30S ribosomal protein S7 (NCBI) compare
b3495 uspA +0.8 6.9 universal stress global response regulator (NCBI) compare
b1243 oppA +0.8 5.9 oligopeptide transporter subunit (NCBI) compare
b4701 sokX +0.8 0.9 sok-related sRNA, function unknown (RefSeq) compare
b3181 greA +0.8 5.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2285 nuoE +0.8 2.9 NADH dehydrogenase subunit E (NCBI) compare
b1247 oppF +0.8 5.9 oligopeptide transporter subunit (NCBI) compare
b2284 nuoF +0.8 4.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2281 nuoI +0.8 3.2 NADH dehydrogenase subunit I (NCBI) compare
b2502 ppx +0.8 5.9 exopolyphosphatase (NCBI) compare
b2904 gcvH +0.8 2.7 glycine cleavage system protein H (NCBI) compare
b4133 cadC +0.8 7.0 DNA-binding transcriptional activator (NCBI) compare
b2189 proL +0.8 2.5 tRNA-Pro (NCBI) compare
b3746 yieN +0.8 6.8 putative 2-component regulator (VIMSS) compare
b0746 valZ +0.8 2.9 tRNA-Val (NCBI) compare
b0218 yafU +0.8 3.1 predicted inner membrane protein (RefSeq) compare
b0092 ddlB +0.7 3.7 D-alanylalanine synthetase (NCBI) compare
b1642 slyA +0.7 2.5 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2911 ssrS +0.7 1.8 6S regulatory RNA (NCBI) compare
b2277 nuoM +0.7 4.2 NADH dehydrogenase subunit M (NCBI) compare
b1187 fadR +0.7 4.2 fatty acid metabolism regulator (NCBI) compare
b2287 nuoB +0.7 3.4 NADH dehydrogenase subunit B (NCBI) compare
b1823 cspC +0.7 3.1 stress protein, member of the CspA-family (NCBI) compare
b2916 argP +0.7 4.5 chromosome replication initiation inhibitor protein (NCBI) compare
b2659 b2659 +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b4232 fbp +0.7 5.6 fructose-1,6-bisphosphatase (NCBI) compare
b4503 yafF +0.7 1.0 no description compare
b4222 ytfP +0.7 4.2 hypothetical protein (NCBI) compare
b1950 fliR +0.6 2.9 flagellar biosynthesis protein R (NCBI) compare
b4313 fimE +0.6 5.3 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2283 nuoG +0.6 4.6 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b3816 corA +0.6 4.9 magnesium/nickel/cobalt transporter (NCBI) compare
b0435 bolA +0.6 4.5 possible regulator of murein genes (VIMSS) compare
b1658 purR +0.6 4.8 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0104 guaC +0.6 4.7 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b1572 ydfB +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b1244 oppB +0.6 4.7 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1258 yciF +0.6 0.8 hypothetical protein (NCBI) compare
b0617 citD +0.6 2.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2814 metZ +0.6 1.6 tRNA-Met (NCBI) compare
b1332 ynaJ +0.6 0.6 predicted inner membrane protein (NCBI) compare
b1790 yeaM +0.6 2.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1648 b1648 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b4522 yciX_1 +0.5 2.5 hypothetical protein (NCBI) compare
b1156 tfaE +0.5 2.8 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b2286 nuoC +0.5 2.6 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3166 truB +0.5 4.3 tRNA pseudouridine synthase B (NCBI) compare
b4511 ybdZ +0.5 2.7 hypothetical protein (NCBI) compare
b2280 nuoJ +0.5 3.2 NADH dehydrogenase subunit J (NCBI) compare
b3516 gadX +0.5 4.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2051 wcaH +0.5 1.4 GDP-mannose mannosyl hydrolase (VIMSS) compare
b3193 yrbD +0.5 4.2 predicted ABC-type organic solvent transporter (NCBI) compare
b1685 ydiH +0.5 1.3 predicted protein (RefSeq) compare
b1246 oppD +0.5 3.6 oligopeptide transporter ATP-binding component (NCBI) compare
b2743 pcm +0.5 3.1 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0082 mraW +0.5 1.8 S-adenosyl-methyltransferase (NCBI) compare
b4126 yjdI +0.5 4.0 hypothetical protein (NCBI) compare
b0494 tesA +0.5 3.0 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b0535 fimZ +0.5 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2288 nuoA +0.5 2.0 NADH dehydrogenase I chain A (VIMSS) compare
b0636 ybeA +0.5 2.5 hypothetical protein (NCBI) compare
b4369 leuP +0.5 0.9 tRNA-Leu (NCBI) compare
b1639 ydhA +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b3146 yraL +0.5 3.0 predicted methyltransferase (NCBI) compare
b1362 rzpR +0.5 2.0 putative Rac prophage endopeptidase (VIMSS) compare
b1689 b1689 +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b4519 icdC +0.4 1.9 no description compare
b2946 yggJ +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b2110 yehC +0.4 0.9 predicted periplasmic pilin chaperone (NCBI) compare
b2939 yqgB +0.4 0.9 predicted protein (RefSeq) compare
b1493 gadB +0.4 0.6 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1245 oppC +0.4 3.2 oligopeptide transporter subunit (NCBI) compare
b1186 nhaB +0.4 3.1 NhaB sodium/proton transporter (Katherine Huang) compare
b4089 rpiR +0.4 3.7 transcriptional repressor of rpiB expression (VIMSS) compare
b1360 ydaV +0.4 0.8 Rac prophage; predicted DNA replication protein (NCBI) compare
b4541 yehK +0.4 2.2 hypothetical protein (NCBI) compare
b1284 yciT +0.4 2.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2092 gatC +0.4 2.0 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b0580 ybdJ +0.4 1.5 predicted inner membrane protein (NCBI) compare
b3074 ygjH +0.4 2.3 hypothetical protein (NCBI) compare
b1394 paaG +0.4 1.6 enoyl-CoA hydratase (NCBI) compare
b1557 cspB +0.4 1.5 Qin prophage; cold shock protein (NCBI) compare
b2637 yfjT +0.4 2.1 CP4-57 prophage; predicted protein (NCBI) compare
b0018 mokC +0.4 0.9 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b0864 artP +0.4 1.9 arginine transporter subunit (NCBI) compare
b0473 htpG +0.4 3.3 heat shock protein 90 (NCBI) compare
b1400 paaY +0.4 1.5 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b3870 glnA +0.4 1.6 glutamine synthetase (NCBI) compare
b3658 selC +0.4 1.9 tRNA-Sec (NCBI) compare
b3810 yigA +0.4 3.1 hypothetical protein (NCBI) compare
b2642 yfjW +0.4 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2282 nuoH +0.4 1.7 NADH dehydrogenase subunit H (NCBI) compare
b1959 yedA +0.4 2.5 predicted inner membrane protein (NCBI) compare
b0862 artQ +0.4 2.3 arginine transporter subunit (NCBI) compare
b4214 cysQ +0.4 3.1 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2903 gcvP +0.4 3.4 glycine dehydrogenase (NCBI) compare
b2531 iscR +0.4 0.6 DNA-binding transcriptional repressor (NCBI) compare
b1582 ynfA +0.4 1.2 hypothetical protein (NCBI) compare
b2708 gutQ +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b1242 ychE +0.4 1.9 predicted inner membrane protein (NCBI) compare
b4642 yoeD +0.4 1.2 no description compare
b0927 ycbL +0.4 1.9 predicted metal-binding enzyme (NCBI) compare
b2225 yfaP +0.4 2.1 hypothetical protein (NCBI) compare
b2824 ygdB +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b0881 clpS +0.4 1.0 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b0289 yagV +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b4543 ypaA +0.4 1.7 predicted protein (NCBI) compare
b2258 b2258 +0.4 0.9 putative transport/receptor protein (VIMSS) compare
b2620 smpB +0.4 0.5 SsrA-binding protein (NCBI) compare
b2346 vacJ +0.4 2.6 predicted lipoprotein (NCBI) compare
b1431 ydcL +0.4 1.4 predicted lipoprotein (NCBI) compare
b2634 yfjR +0.4 1.4 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0014 dnaK +0.4 1.0 molecular chaperone DnaK (NCBI) compare
b4041 plsB +0.4 1.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2829 ptsP +0.4 3.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b0251 yafY +0.4 1.7 CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0556 rzpD +0.4 1.4 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b1333 uspE +0.4 1.5 stress-induced protein (NCBI) compare
b4403 yjtD +0.4 2.5 predicted rRNA methyltransferase (NCBI) compare
b1183 umuD +0.3 0.8 DNA polymerase V, subunit D (NCBI) compare
b2421 cysM +0.3 2.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b3443 yrhA +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1814 sdaA +0.3 1.5 L-serine deaminase I (NCBI) compare
b3280 yrdB +0.3 2.3 hypothetical protein (NCBI) compare
b0852 rimK +0.3 2.1 ribosomal protein S6 modification protein (NCBI) compare
b0618 citC +0.3 2.5 citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS) compare
b3206 npr +0.3 2.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1709 btuD +0.3 0.6 vitamin B12-transporter ATPase (NCBI) compare
b2094 gatA +0.3 2.1 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b1418 cybB +0.3 0.9 cytochrome b(561) (VIMSS) compare
b4538 yoeF +0.3 1.1 hypothetical protein (RefSeq) compare
b4552 yrhC +0.3 0.6 no description compare
b1612 fumA +0.3 1.6 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b4633 xisD +0.3 1.6 no description compare
b0863 artI +0.3 1.7 arginine transporter subunit (NCBI) compare
b2734 pphB +0.3 1.8 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b4068 yjcH +0.3 1.4 required for glycolate transport, with actP (from data) compare
b1960 vsr +0.3 1.9 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1840 yebZ +0.3 2.0 predicted inner membrane protein (NCBI) compare
b0778 bioD +0.3 1.3 dethiobiotin synthetase (NCBI) compare
b2236 yfaE +0.3 1.5 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3397 nudE +0.3 2.5 ADP-ribose diphosphatase (NCBI) compare
b0840 deoR +0.3 2.2 DNA-binding transcriptional repressor (NCBI) compare
b1883 cheB +0.3 2.2 chemotaxis-specific methylesterase (NCBI) compare
b3192 yrbC +0.3 2.7 predicted ABC-type organic solvent transporter (NCBI) compare
b4337 yjiO +0.3 2.4 multidrug efflux system protein (NCBI) compare
b0364 yaiS +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b0952 ymbA +0.3 2.2 conserved protein (RefSeq) compare
b0710 ybgI +0.3 1.7 conserved metal-binding protein (NCBI) compare
b4391 yjjK +0.3 2.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1573 ydfC +0.3 0.6 Qin prophage; predicted protein (RefSeq) compare
b2812 ygdL +0.3 2.4 hypothetical protein (NCBI) compare
b1231 tyrT +0.3 0.6 tRNA-Tyr (NCBI) compare
b3636 rpmG +0.3 0.9 50S ribosomal protein L33 (NCBI) compare
b1829 htpX +0.3 2.3 heat shock protein HtpX (NCBI) compare
b2278 nuoL +0.3 2.2 NADH dehydrogenase subunit L (NCBI) compare
b2005 yeeV +0.3 1.6 CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1261 trpB +0.3 1.4 tryptophan synthase subunit beta (NCBI) compare
b0013 yaaI +0.3 1.9 hypothetical protein (NCBI) compare
b3191 yrbB +0.3 2.4 orf, hypothetical protein (VIMSS) compare
b1921 fliZ +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0340 cynS +0.3 1.5 cyanate hydratase (NCBI) compare
b4207 fklB +0.3 2.1 FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS) compare
b1563 relE +0.3 0.7 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b4536 yobH +0.3 1.4 hypothetical protein (NCBI) compare
b4233 mpl +0.3 2.6 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI) compare
b0546 ybcM +0.3 1.7 DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1824 yobF +0.3 1.4 hypothetical protein (NCBI) compare
b1841 yobA +0.3 0.7 hypothetical protein (NCBI) compare
b2212 alkB +0.3 1.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b0706 ybfD +0.3 1.0 hypothetical protein (NCBI) compare
b0931 pncB +0.3 1.8 nicotinate phosphoribosyltransferase (NCBI) compare
b4598 yncL +0.3 1.0 hypothetical protein (RefSeq) compare
b3195 yrbF +0.3 2.5 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1103 ycfF +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b4097 phnK +0.3 1.9 carbon-phosphorus lyase complex subunit (NCBI) compare
b4406 yaeP +0.3 1.3 hypothetical protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Sulfasalazine in Escherichia coli BW25113

For stress Sulfasalazine across organisms