Experiment set56IT081 for Escherichia coli BW25113

LB Anaerobic Sulfasalazine 250 uM

Group: stress
Media: LB + Sulfasalazine (250 uM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Lindsey on 2/15/22
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 4 genes in this experiment

For stress Sulfasalazine in Escherichia coli BW25113

For stress Sulfasalazine across organisms

SEED Subsystems

Subsystem	#Specific
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
spermine and spermidine degradation I	5	2	2
5-(methoxycarbonylmethoxy)uridine biosynthesis	3	3	1
putrescine degradation III	4	2	1