Experiment set56IT080 for Escherichia coli BW25113

Compare to:

LB Anaerobic Sulfasalazine 250 uM

200 most detrimental genes:

  gene name fitness t score description  
b3341 rpsG +1.6 3.9 30S ribosomal protein S7 (NCBI) compare
b2217 rcsB +1.5 10.1 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3390 aroK +1.5 4.1 shikimate kinase I (NCBI) compare
b3979 thrT +1.4 0.9 tRNA-Thr (NCBI) compare
b2289 lrhA +1.3 9.4 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b2642 yfjW +1.2 2.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3512 GadE +1.2 4.7 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b2096 gatY +1.1 6.0 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1361 ydaW +1.1 1.0 orf, hypothetical protein (VIMSS) compare
b2189 proL +1.0 3.6 tRNA-Pro (NCBI) compare
b2156 lysP +1.0 9.0 lysine transporter (NCBI) compare
b2285 nuoE +1.0 3.7 NADH dehydrogenase subunit E (NCBI) compare
b3779 gpp +1.0 9.2 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b2934 cmtB +1.0 1.3 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1332 ynaJ +1.0 0.8 predicted inner membrane protein (NCBI) compare
b2279 nuoK +1.0 1.9 NADH dehydrogenase subunit K (NCBI) compare
b4699 fnrS +1.0 1.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3375 yhfR +1.0 6.9 putative transcriptional regulator (VIMSS) compare
b3183 obgE +1.0 1.6 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1492 gadC +0.9 5.5 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b2093 gatB +0.9 4.9 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2853 ygeI +0.9 0.6 hypothetical protein (NCBI) compare
b3181 greA +0.9 5.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2939 yqgB +0.9 2.4 predicted protein (RefSeq) compare
b1950 fliR +0.9 3.9 flagellar biosynthesis protein R (NCBI) compare
b1243 oppA +0.9 6.2 oligopeptide transporter subunit (NCBI) compare
b3495 uspA +0.9 7.5 universal stress global response regulator (NCBI) compare
b2281 nuoI +0.9 3.0 NADH dehydrogenase subunit I (NCBI) compare
b0955 ycbZ +0.8 6.3 predicted peptidase (NCBI) compare
b1465 narV +0.8 1.3 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b2502 ppx +0.8 5.6 exopolyphosphatase (NCBI) compare
b3745 yieM +0.8 7.4 predicted von Willibrand factor containing protein (NCBI) compare
b2236 yfaE +0.8 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1244 oppB +0.8 5.5 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2916 argP +0.8 5.1 chromosome replication initiation inhibitor protein (NCBI) compare
b2946 yggJ +0.8 4.2 orf, hypothetical protein (VIMSS) compare
b3746 yieN +0.8 6.9 putative 2-component regulator (VIMSS) compare
b2531 iscR +0.8 1.7 DNA-binding transcriptional repressor (NCBI) compare
b1493 gadB +0.8 1.1 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b2095 gatZ +0.7 6.3 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b3816 corA +0.7 5.6 magnesium/nickel/cobalt transporter (NCBI) compare
b0092 ddlB +0.7 4.6 D-alanylalanine synthetase (NCBI) compare
b4133 cadC +0.7 6.5 DNA-binding transcriptional activator (NCBI) compare
b4503 yafF +0.7 1.1 no description compare
b2277 nuoM +0.7 4.6 NADH dehydrogenase subunit M (NCBI) compare
b1346 ydaQ +0.7 1.7 orf, hypothetical protein (VIMSS) compare
b3107 yhaL +0.7 1.9 orf, hypothetical protein (VIMSS) compare
b4313 fimE +0.7 5.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4041 plsB +0.7 2.3 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1778 yeaA +0.7 2.0 methionine sulfoxide reductase B (NCBI) compare
b4451 ryhB +0.7 1.3 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0191 yaeJ +0.7 2.9 hypothetical protein (NCBI) compare
b1642 slyA +0.7 1.9 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4232 fbp +0.7 5.5 fructose-1,6-bisphosphatase (NCBI) compare
b0746 valZ +0.7 2.8 tRNA-Val (NCBI) compare
b0247 ykfG +0.7 1.5 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0104 guaC +0.7 4.5 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b0435 bolA +0.6 3.6 possible regulator of murein genes (VIMSS) compare
b1648 b1648 +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b4592 yccB +0.6 2.0 hypothetical protein (RefSeq) compare
b1025 ycdT +0.6 1.2 predicted diguanylate cyclase (NCBI) compare
b0014 dnaK +0.6 2.1 molecular chaperone DnaK (NCBI) compare
b0502 ylbG +0.6 2.0 no description compare
b2286 nuoC +0.6 3.8 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1518 lsrG +0.6 1.5 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b0218 yafU +0.6 2.2 predicted inner membrane protein (RefSeq) compare
b3166 truB +0.6 4.1 tRNA pseudouridine synthase B (NCBI) compare
b3146 yraL +0.6 3.9 predicted methyltransferase (NCBI) compare
b1187 fadR +0.6 4.2 fatty acid metabolism regulator (NCBI) compare
b1245 oppC +0.6 3.2 oligopeptide transporter subunit (NCBI) compare
b2421 cysM +0.6 3.1 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b4570 lomR +0.6 1.3 no description compare
b4577 sgrS +0.6 1.6 small antisense RNA (NCBI) compare
b3027 ygiZ +0.5 0.7 conserved inner membrane protein (NCBI) compare
b2283 nuoG +0.5 3.7 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b3516 gadX +0.5 4.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2904 gcvH +0.5 2.0 glycine cleavage system protein H (NCBI) compare
b4522 yciX_1 +0.5 2.3 hypothetical protein (NCBI) compare
b4345 mcrC +0.5 1.0 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1758 b1758 +0.5 1.2 putative cytochrome oxidase (VIMSS) compare
b2343 b2343 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1658 purR +0.5 3.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0726 sucA +0.5 2.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b2659 b2659 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b4416 rybA +0.5 1.3 unknown RNA (NCBI) compare
b1165 ymgA +0.5 0.5 hypothetical protein (NCBI) compare
b3238 yhcN +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1841 yobA +0.5 2.0 hypothetical protein (NCBI) compare
b3193 yrbD +0.5 3.9 predicted ABC-type organic solvent transporter (NCBI) compare
b1370 insH-5 +0.5 0.8 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b0617 citD +0.5 1.2 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2110 yehC +0.5 1.0 predicted periplasmic pilin chaperone (NCBI) compare
b2903 gcvP +0.5 4.2 glycine dehydrogenase (NCBI) compare
b4126 yjdI +0.5 4.2 hypothetical protein (NCBI) compare
b4222 ytfP +0.5 3.6 hypothetical protein (NCBI) compare
b1821 b1821 +0.5 2.7 orf, hypothetical protein (VIMSS) compare
b1164 ycgZ +0.5 0.9 hypothetical protein (NCBI) compare
b2498 upp +0.5 1.8 uracil phosphoribosyltransferase (VIMSS) compare
b2094 gatA +0.5 3.1 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b3206 npr +0.5 2.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2911 ssrS +0.5 0.9 6S regulatory RNA (NCBI) compare
b3101 yqjF +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1246 oppD +0.5 2.6 oligopeptide transporter ATP-binding component (NCBI) compare
b4551 yheV +0.5 1.2 hypothetical protein (NCBI) compare
b2765 ygcM +0.5 2.1 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b3870 glnA +0.5 1.5 glutamine synthetase (NCBI) compare
b0931 pncB +0.4 2.4 nicotinate phosphoribosyltransferase (NCBI) compare
b1827 kdgR +0.4 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4596 yciZ +0.4 1.5 hypothetical protein (RefSeq) compare
b1233 ychJ +0.4 2.1 hypothetical protein (NCBI) compare
b1765 ydjA +0.4 0.7 predicted oxidoreductase (NCBI) compare
b2540 hcaC +0.4 2.6 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b3889 yiiE +0.4 1.4 predicted transcriptional regulator (RefSeq) compare
b1161 ycgX +0.4 0.7 hypothetical protein (NCBI) compare
b4214 cysQ +0.4 3.6 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0013 yaaI +0.4 2.5 hypothetical protein (NCBI) compare
b1431 ydcL +0.4 1.5 predicted lipoprotein (NCBI) compare
b4402 yjjY +0.4 0.8 hypothetical protein (NCBI) compare
b0636 ybeA +0.4 2.1 hypothetical protein (NCBI) compare
b0862 artQ +0.4 1.1 arginine transporter subunit (NCBI) compare
b1247 oppF +0.4 1.5 oligopeptide transporter subunit (NCBI) compare
b1028 ymdE +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b1959 yedA +0.4 2.2 predicted inner membrane protein (NCBI) compare
b2291 yfbR +0.4 2.5 hypothetical protein (NCBI) compare
b1182 hlyE +0.4 0.4 hemolysin E (VIMSS) compare
b0426 yajQ +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b2346 vacJ +0.4 2.4 predicted lipoprotein (NCBI) compare
b3810 yigA +0.4 3.2 hypothetical protein (NCBI) compare
b4181 yjfI +0.4 1.5 hypothetical protein (NCBI) compare
b3228 sspB +0.4 1.9 ClpXP protease specificity-enhancing factor (NCBI) compare
b2197 ccmE +0.4 2.5 periplasmic heme chaperone (NCBI) compare
b4436 ryeC +0.4 1.1 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b0461 ybaJ +0.4 3.0 hypothetical protein (NCBI) compare
b1448 yncA +0.4 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2287 nuoB +0.4 1.9 NADH dehydrogenase subunit B (NCBI) compare
b1261 trpB +0.4 1.6 tryptophan synthase subunit beta (NCBI) compare
b3397 nudE +0.4 3.2 ADP-ribose diphosphatase (NCBI) compare
b4504 ykfH +0.4 2.6 hypothetical protein (NCBI) compare
b4571 wbbL +0.4 1.7 no description compare
b0710 ybgI +0.4 1.8 conserved metal-binding protein (NCBI) compare
b1527 yneK +0.4 1.4 hypothetical protein (NCBI) compare
b2284 nuoF +0.4 2.0 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b4541 yehK +0.4 2.2 hypothetical protein (NCBI) compare
b0233 yafO +0.4 2.3 predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2743 pcm +0.4 2.4 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0863 artI +0.4 2.5 arginine transporter subunit (NCBI) compare
b3195 yrbF +0.4 3.1 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3033 yqiB +0.4 1.7 predicted dehydrogenase (NCBI) compare
b2476 purC +0.4 1.3 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b0082 mraW +0.4 1.7 S-adenosyl-methyltransferase (NCBI) compare
b2479 gcvR +0.4 1.9 transcriptional regulation of gcv operon (VIMSS) compare
b4116 adiY +0.4 3.2 DNA-binding transcriptional activator (NCBI) compare
b0357 yaiN +0.4 1.3 putative alpha helix chain (VIMSS) compare
b2200 ccmB +0.4 1.8 heme exporter subunit (NCBI) compare
b0828 iaaA +0.4 1.4 L-asparaginase (NCBI) compare
b1951 rcsA +0.4 0.7 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2203 napB +0.4 2.5 cytochrome c-type protein (VIMSS) compare
b0610 rnk +0.4 1.5 nucleoside diphosphate kinase regulator (NCBI) compare
b0473 htpG +0.4 3.0 heat shock protein 90 (NCBI) compare
b0001 thrL +0.4 1.6 thr operon leader peptide (NCBI) compare
b4089 rpiR +0.4 3.1 transcriptional repressor of rpiB expression (VIMSS) compare
b3074 ygjH +0.4 1.8 hypothetical protein (NCBI) compare
b0210 yafE +0.4 1.2 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b0778 bioD +0.4 1.5 dethiobiotin synthetase (NCBI) compare
b1530 marR +0.4 1.8 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1842 holE +0.4 0.8 DNA polymerase III, theta subunit (NCBI) compare
b3207 yrbL +0.4 2.0 hypothetical protein (NCBI) compare
b3443 yrhA +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b2455 eutE +0.4 2.0 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b1289 ycjD +0.4 1.6 hypothetical protein (NCBI) compare
b0494 tesA +0.4 2.4 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b2820 recB +0.4 0.4 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3192 yrbC +0.4 3.0 predicted ABC-type organic solvent transporter (NCBI) compare
b1284 yciT +0.3 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1171 b1171 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b2959 yggL +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b0471 ybaB +0.3 1.1 hypothetical protein (NCBI) compare
b0318 yahD +0.3 1.9 predicted transcriptional regulator with ankyrin domain (NCBI) compare
b1362 rzpR +0.3 1.0 putative Rac prophage endopeptidase (VIMSS) compare
b1703 ydiA +0.3 1.9 hypothetical protein (NCBI) compare
b1110 ycfJ +0.3 2.7 hypothetical protein (NCBI) compare
b0736 ybgC +0.3 1.5 predicted acyl-CoA thioesterase (NCBI) compare
b3057 bacA +0.3 2.3 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b4049 dusA +0.3 2.6 tRNA-dihydrouridine synthase A (RefSeq) compare
b2007 yeeX +0.3 1.2 conserved protein (RefSeq) compare
b2276 nuoN +0.3 1.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b2092 gatC +0.3 1.5 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b1524 yneH +0.3 0.6 predicted glutaminase (NCBI) compare
b1263 trpD +0.3 1.4 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b3636 rpmG +0.3 0.8 50S ribosomal protein L33 (NCBI) compare
b1884 cheR +0.3 1.5 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b2762 cysH +0.3 2.2 phosphoadenosine phosphosulfate reductase (NCBI) compare
b4387 ytjB +0.3 2.1 hypothetical protein (NCBI) compare
b1186 nhaB +0.3 2.5 NhaB sodium/proton transporter (Katherine Huang) compare
b2829 ptsP +0.3 2.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2741 rpoS +0.3 2.1 RNA polymerase sigma factor (NCBI) compare
b1789 yeaL +0.3 1.5 conserved inner membrane protein (NCBI) compare
b1960 vsr +0.3 1.2 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1548 nohA +0.3 0.9 Qin prophage; predicted packaging protein (RefSeq) compare
b0996 torC +0.3 2.2 trimethylamine N-oxide (TMAO) reductase I, cytochrome c-type subunit (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Sulfasalazine in Escherichia coli BW25113

For stress Sulfasalazine across organisms