Experiment set56IT075 for Escherichia coli BW25113

Compare to:

M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM

200 most important genes:

  gene name fitness t score description  
b3960 argH -5.0 -19.3 argininosuccinate lyase (NCBI) compare
b3172 argG -4.9 -15.6 argininosuccinate synthase (NCBI) compare
b0523 purE -4.8 -7.1 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b2838 lysA -4.4 -15.6 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b0003 thrB -4.4 -8.8 homoserine kinase (NCBI) compare
b1281 pyrF -4.3 -3.0 orotidine 5'-phosphate decarboxylase (NCBI) compare
b2557 purL -4.3 -17.3 phosphoribosylformylglycinamidine synthase (NCBI) compare
b0907 serC -4.2 -9.5 phosphoserine aminotransferase (NCBI) compare
b0522 purK -4.1 -13.9 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b2476 purC -4.1 -6.2 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b0004 thrC -4.0 -13.5 threonine synthase (NCBI) compare
b2312 purF -3.9 -8.9 amidophosphoribosyltransferase (NCBI) compare
b0908 aroA -3.8 -13.4 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4388 serB -3.7 -8.8 3-phosphoserine phosphatase (NCBI) compare
b4005 purD -3.7 -12.1 phosphoribosylamine--glycine ligase (NCBI) compare
b4006 purH -3.7 -14.8 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b2913 serA -3.5 -12.6 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b1062 pyrC -3.4 -5.6 dihydroorotase (NCBI) compare
b3642 pyrE -3.1 -2.2 orotate phosphoribosyltransferase (NCBI) compare
b0222 lpcA -3.1 -2.1 phosphoheptose isomerase (NCBI) compare
b0002 thrA -3.1 -14.6 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b3870 glnA -3.0 -3.5 glutamine synthetase (NCBI) compare
b0145 dksA -2.9 -3.9 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b2501 ppk -2.9 -11.9 polyphosphate kinase (NCBI) compare
b1260 trpA -2.9 -8.7 tryptophan synthase subunit alpha (NCBI) compare
b2499 purM -2.8 -7.2 phosphoribosylaminoimidazole synthetase (NCBI) compare
b0049 apaH -2.8 -1.9 diadenosinetetraphosphatase (NCBI) compare
b3956 ppc -2.8 -19.1 phosphoenolpyruvate carboxylase (NCBI) compare
b1261 trpB -2.7 -5.1 tryptophan synthase subunit beta (NCBI) compare
b1882 cheY -2.7 -1.1 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2564 pdxJ -2.7 -7.9 pyridoxal phosphate biosynthetic protein (NCBI) compare
b2329 aroC -2.7 -8.9 chorismate synthase (NCBI) compare
b0386 proC -2.7 -3.6 pyrroline-5-carboxylate reductase (NCBI) compare
b2318 truA -2.6 -4.0 tRNA pseudouridine synthase A (NCBI) compare
b2188 yejM -2.5 -13.7 predicted hydrolase, inner membrane (NCBI) compare
b2808 gcvA -2.5 -5.7 DNA-binding transcriptional dual regulator (NCBI) compare
b3389 aroB -2.5 -12.6 3-dehydroquinate synthase (NCBI) compare
b3281 aroE -2.4 -15.4 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0945 pyrD -2.4 -4.3 dihydroorotate dehydrogenase (NCBI) compare
b0564 appY -2.4 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0032 carA -2.4 -4.2 carbamoyl-phosphate synthase small subunit (NCBI) compare
b4214 cysQ -2.3 -15.1 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b3052 rfaE -2.3 -3.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4245 pyrB -2.3 -3.1 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b4702 mgtL -2.2 -1.5 regulatory leader peptide for mgtA (NCBI) compare
b2762 cysH -2.2 -12.1 phosphoadenosine phosphosulfate reductase (NCBI) compare
b3790 rffC -2.1 -4.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b0052 pdxA -2.1 -12.8 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b2912 ygfA -2.1 -2.8 putative ligase (VIMSS) compare
b1830 prc -2.1 -4.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b4481 rffT -2.0 -5.1 4-alpha-L-fucosyltransferase (NCBI) compare
b2320 pdxB -2.0 -12.0 erythronate-4-phosphate dehydrogenase (NCBI) compare
b0438 clpX -2.0 -2.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3621 rfaC -2.0 -1.9 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b4025 pgi -2.0 -10.7 glucose-6-phosphate isomerase (NCBI) compare
b4242 mgtA -1.8 -7.9 magnesium transporter (NCBI) compare
b1693 aroD -1.8 -7.4 3-dehydroquinate dehydratase (NCBI) compare
b0033 carB -1.8 -5.0 carbamoyl-phosphate synthase large subunit (NCBI) compare
b3916 pfkA -1.8 -3.1 6-phosphofructokinase (NCBI) compare
b2839 lysR -1.7 -5.0 DNA-binding transcriptional dual regulator (NCBI) compare
b0688 pgm -1.7 -3.5 phosphoglucomutase (NCBI) compare
b1273 yciN -1.7 -2.4 hypothetical protein (NCBI) compare
b1192 ldcA -1.7 -3.3 L,D-carboxypeptidase A (NCBI) compare
b0720 gltA -1.7 -8.0 citrate synthase (NCBI) compare
b3620 rfaF -1.7 -3.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2175 spr -1.7 -3.1 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3829 metE -1.7 -13.9 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b0051 ksgA -1.6 -5.1 dimethyladenosine transferase (NCBI) compare
b1185 dsbB -1.6 -3.4 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0632 dacA -1.6 -8.7 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2832 ygdQ -1.6 -10.0 predicted inner membrane protein (NCBI) compare
b3941 metF -1.6 -10.6 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b0889 lrp -1.6 -4.4 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3787 rffD -1.6 -8.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4171 miaA -1.6 -2.9 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2750 cysC -1.6 -6.8 adenylylsulfate kinase (NCBI) compare
b2414 cysK -1.6 -9.5 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b4146 yjeK -1.5 -2.0 predicted lysine aminomutase (NCBI) compare
b3164 pnp -1.5 -3.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1438 ydcQ -1.5 -2.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0928 aspC -1.5 -7.1 aspartate aminotransferase, PLP-dependent (NCBI) compare
b0926 ycbK -1.5 -7.5 hypothetical protein (NCBI) compare
b0730 mngR -1.5 -2.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1865 nudB -1.5 -2.1 dATP pyrophosphohydrolase (NCBI) compare
b3619 rfaD -1.5 -1.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3838 tatB -1.4 -6.7 sec-independent translocase (NCBI) compare
b2022 hisB -1.4 -6.8 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b4218 ytfL -1.4 -7.8 predicted inner membrane protein (NCBI) compare
b3786 rffE -1.4 -8.9 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3229 sspA -1.4 -4.3 stringent starvation protein A (NCBI) compare
b2784 relA -1.4 -7.9 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2023 hisH -1.4 -6.3 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3828 metR -1.4 -9.4 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b3924 fpr -1.3 -8.7 ferredoxin-NADP reductase (NCBI) compare
b3082 ygjM -1.3 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2600 tyrA -1.3 -7.7 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b2567 rnc -1.3 -0.8 ribonuclease III (NCBI) compare
b3774 ilvC -1.3 -10.5 ketol-acid reductoisomerase (NCBI) compare
b2764 cysJ -1.2 -7.7 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b1231 tyrT -1.2 -1.5 tRNA-Tyr (NCBI) compare
b2064 asmA -1.2 -5.6 predicted assembly protein (NCBI) compare
b4375 prfC -1.2 -4.8 peptide chain release factor 3 (NCBI) compare
b3021 ygiT -1.2 -2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b0437 clpP -1.2 -0.8 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3741 gidA -1.2 -2.1 glucose-inhibited division protein A (NCBI) compare
b3791 rffA -1.2 -4.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0242 proB -1.2 -7.0 gamma-glutamyl kinase (NCBI) compare
b0200 gmhB -1.2 -0.8 hypothetical protein (NCBI) compare
b3771 ilvD -1.2 -9.2 dihydroxy-acid dehydratase (NCBI) compare
b2763 cysI -1.2 -6.6 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b1850 eda -1.2 -1.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b4246 pyrL -1.1 -1.0 pyrBI operon leader peptide (NCBI) compare
b1176 minC -1.1 -3.1 septum formation inhibitor (NCBI) compare
b1863 ruvC -1.1 -1.0 Holliday junction resolvase (NCBI) compare
b3860 dsbA -1.1 -5.8 periplasmic protein disulfide isomerase I (NCBI) compare
b1150 ymfR -1.1 -1.4 e14 prophage; predicted protein (NCBI) compare
b0243 proA -1.1 -7.5 gamma-glutamyl phosphate reductase (NCBI) compare
b0088 murD -1.1 -2.8 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3613 yibP -1.1 -1.4 putative membrane protein (VIMSS) compare
b2313 cvpA -1.1 -4.4 membrane protein required for colicin V production (NCBI) compare
b2818 argA -1.1 -6.8 N-acetylglutamate synthase (NCBI) compare
b2024 hisA -1.1 -2.0 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b3839 tatC -1.1 -2.2 TatABCE protein translocation system subunit (NCBI) compare
b2020 hisD -1.1 -6.6 histidinol dehydrogenase (NCBI) compare
b3957 argE -1.1 -8.3 acetylornithine deacetylase (NCBI) compare
b3792 wzxE -1.1 -2.7 O-antigen translocase (NCBI) compare
b0957 ompA -1.0 -3.1 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b1084 rne -1.0 -3.7 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3958 argC -1.0 -7.6 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b3959 argB -1.0 -7.4 acetylglutamate kinase (RefSeq) compare
b1262 trpC -1.0 -3.6 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b2479 gcvR -1.0 -5.7 transcriptional regulation of gcv operon (VIMSS) compare
b2504 yfgG -1.0 -1.3 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b2021 hisC -1.0 -3.5 histidinol-phosphate aminotransferase (NCBI) compare
b1936 intG -1.0 -1.5 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0912 ihfB -1.0 -0.6 integration host factor subunit beta (NCBI) compare
b3609 secB -1.0 -2.2 export protein SecB (NCBI) compare
b4414 tff -1.0 -1.4 unknown RNA (NCBI) compare
b4655 ythA -1.0 -1.5 expressed protein (RefSeq) compare
b0729 sucD -0.9 -2.4 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3977 tyrU -0.9 -0.8 tRNA-Tyr (NCBI) compare
b1862 yebB -0.9 -3.7 orf, hypothetical protein (VIMSS) compare
b3770 ilvE -0.9 -7.2 branched-chain amino acid aminotransferase (NCBI) compare
b2516 yfgA -0.9 -4.6 hypothetical protein (NCBI) compare
b3027 ygiZ -0.9 -0.6 conserved inner membrane protein (NCBI) compare
b3368 cysG -0.9 -7.7 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b1346 ydaQ -0.9 -1.6 orf, hypothetical protein (VIMSS) compare
b3469 zntA -0.9 -5.7 zinc, cobalt and lead efflux system (NCBI) compare
b0053 surA -0.9 -2.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2620 smpB -0.9 -0.8 SsrA-binding protein (NCBI) compare
b0142 folK -0.9 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2935 tktA -0.9 -1.5 transketolase 1, thiamin-binding (NCBI) compare
b1506 yneL -0.9 -1.7 predicted transcriptional regulator (NCBI) compare
b1235 rssB -0.9 -3.1 response regulator of RpoS (NCBI) compare
b3911 cpxA -0.9 -5.4 two-component sensor protein (NCBI) compare
b3706 trmE -0.8 -2.6 tRNA modification GTPase (NCBI) compare
b3527 yhjJ -0.8 -7.5 predicted zinc-dependent peptidase (NCBI) compare
b2363 yfdT -0.8 -1.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2025 hisF -0.8 -2.8 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b3794 rffM -0.8 -3.4 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4676 yoaK -0.8 -0.6 expressed protein (RefSeq) compare
b0400 phoR -0.8 -5.3 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b1241 adhE -0.8 -6.0 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b4179 vacB -0.8 -3.7 putative enzyme (VIMSS) compare
b4202 rpsR -0.8 -0.7 30S ribosomal protein S18 (NCBI) compare
b0001 thrL -0.8 -0.8 thr operon leader peptide (NCBI) compare
b1107 nagZ -0.8 -3.4 beta-hexosaminidase (NCBI) compare
b3784 rfe -0.8 -5.2 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b2019 hisG -0.8 -3.5 ATP phosphoribosyltransferase (NCBI) compare
b2683 ygaH -0.8 -2.4 predicted inner membrane protein (NCBI) compare
b1524 yneH -0.8 -1.1 predicted glutaminase (NCBI) compare
b3764 yifE -0.8 -3.0 hypothetical protein (NCBI) compare
b2144 sanA -0.8 -6.1 hypothetical protein (NCBI) compare
b1095 fabF -0.8 -4.4 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1290 sapF -0.8 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0726 sucA -0.7 -3.3 alpha-ketoglutarate decarboxylase (NCBI) compare
b2614 grpE -0.7 -2.3 heat shock protein (NCBI) compare
b2752 cysD -0.7 -3.0 sulfate adenylyltransferase subunit 2 (NCBI) compare
b4513 kdpF -0.7 -1.3 potassium ion accessory transporter subunit (NCBI) compare
b0149 mrcB -0.7 -4.9 penicillin-binding protein 1b (NCBI) compare
b3204 ptsN -0.7 -2.5 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3404 envZ -0.7 -4.7 osmolarity sensor protein (NCBI) compare
b2500 purN -0.7 -3.6 phosphoribosylglycinamide formyltransferase (NCBI) compare
b3375 yhfR -0.7 -4.5 putative transcriptional regulator (VIMSS) compare
b3252 yhdA -0.7 -5.9 conserved inner membrane protein (NCBI) compare
b2599 pheA -0.7 -5.0 fused chorismate mutase P/prephenate dehydratase (NCBI) compare
b2611 ypjD -0.7 -4.0 predicted inner membrane protein (RefSeq) compare
b3237 argR -0.7 -2.4 arginine repressor (NCBI) compare
b2340 sixA -0.7 -2.9 phosphohistidine phosphatase (NCBI) compare
b0075 leuL -0.7 -4.6 leu operon leader peptide (NCBI) compare
b0439 lon -0.7 -2.3 DNA-binding ATP-dependent protease La (NCBI) compare
b1263 trpD -0.7 -1.8 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b4701 sokX -0.7 -0.7 sok-related sRNA, function unknown (RefSeq) compare
b3842 rfaH -0.7 -2.3 transcriptional activator RfaH (NCBI) compare
b1232 purU -0.7 -4.1 formyltetrahydrofolate deformylase (NCBI) compare
b2215 ompC -0.7 -0.5 outer membrane porin protein C (NCBI) compare
b0101 yacG -0.7 -3.0 zinc-binding protein (NCBI) compare
b2017 yefM -0.7 -0.7 orf, hypothetical protein (VIMSS) compare
b2071 yegJ -0.7 -1.7 hypothetical protein (NCBI) compare
b3162 deaD -0.7 -2.7 inducible ATP-independent RNA helicase (VIMSS) compare


Specific Phenotypes

For 3 genes in this experiment

For stress 5-Fluorodihydropyrimidine-2,4-dione in Escherichia coli BW25113

For stress 5-Fluorodihydropyrimidine-2,4-dione across organisms