Experiment set56IT075 for Escherichia coli BW25113

Compare to:

M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM

200 most detrimental genes:

  gene name fitness t score description  
b2642 yfjW +1.1 1.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1291 sapD +1.0 2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4503 yafF +1.0 1.4 no description compare
b2168 fruK +1.0 1.1 1-phosphofructokinase (NCBI) compare
b3341 rpsG +1.0 2.1 30S ribosomal protein S7 (NCBI) compare
b2259 pmrD +1.0 2.4 polymyxin resistance protein B (VIMSS) compare
b0018 mokC +0.9 2.3 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2531 iscR +0.8 2.5 DNA-binding transcriptional repressor (NCBI) compare
b1567 intK +0.8 2.0 Qin prophage; predicted protein (RefSeq) compare
b3119 tdcR +0.8 2.5 threonine dehydratase operon activator protein (VIMSS) compare
b4631 ykgQ +0.8 2.4 no description compare
b0617 citD +0.7 2.5 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1824 yobF +0.7 2.3 hypothetical protein (NCBI) compare
b3390 aroK +0.7 1.4 shikimate kinase I (NCBI) compare
b1607 ydgC +0.7 1.4 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1161 ycgX +0.7 1.2 hypothetical protein (NCBI) compare
b1171 b1171 +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b2217 rcsB +0.6 5.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4596 yciZ +0.6 0.9 hypothetical protein (RefSeq) compare
b2741 rpoS +0.6 4.3 RNA polymerase sigma factor (NCBI) compare
b4369 leuP +0.6 1.0 tRNA-Leu (NCBI) compare
b1370 insH-5 +0.6 1.7 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2433 b2433 +0.6 2.8 orf, hypothetical protein (VIMSS) compare
b3181 greA +0.6 3.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1728 ydjM +0.6 3.0 predicted inner membrane protein regulated by LexA (RefSeq) compare
b4458 oxyS +0.6 0.5 global regulatory RNA (NCBI) compare
b2189 proL +0.6 1.8 tRNA-Pro (NCBI) compare
b4699 fnrS +0.5 0.8 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0843 ybjH +0.5 1.0 hypothetical protein (NCBI) compare
b3206 npr +0.5 2.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1709 btuD +0.5 0.8 vitamin B12-transporter ATPase (NCBI) compare
b4595 yciY +0.5 1.2 hypothetical protein (RefSeq) compare
b3816 corA +0.5 4.0 magnesium/nickel/cobalt transporter (NCBI) compare
b0746 valZ +0.5 2.1 tRNA-Val (NCBI) compare
b1557 cspB +0.5 1.6 Qin prophage; cold shock protein (NCBI) compare
b4568 ytjA +0.5 1.8 predicted protein (RefSeq) compare
b1196 ycgY +0.5 2.4 hypothetical protein (NCBI) compare
b1400 paaY +0.5 2.4 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2277 nuoM +0.5 3.0 NADH dehydrogenase subunit M (NCBI) compare
b0310 ykgH +0.5 1.0 predicted inner membrane protein (NCBI) compare
b1726 yniB +0.5 1.8 predicted inner membrane protein (NCBI) compare
b2765 ygcM +0.5 3.4 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b3382 yhfY +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b2362 yfdS +0.5 2.6 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4201 priB +0.5 1.9 primosomal replication protein N (NCBI) compare
b4603 rseX +0.5 0.7 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0998 torD +0.5 1.1 chaperone protein TorD (NCBI) compare
b1563 relE +0.5 1.7 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b1348 lar +0.5 1.4 Rac prophage; restriction alleviation protein (NCBI) compare
b3875 ompL +0.5 1.6 predicted outer membrane porin L (NCBI) compare
b0191 yaeJ +0.5 3.1 hypothetical protein (NCBI) compare
b1244 oppB +0.5 3.4 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2990 hybG +0.5 1.5 hydrogenase 2 accessory protein (NCBI) compare
b1165 ymgA +0.5 0.4 hypothetical protein (NCBI) compare
b0977 hyaF +0.5 2.3 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b2285 nuoE +0.4 1.5 NADH dehydrogenase subunit E (NCBI) compare
b1007 b1007 +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1754 b1754 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3636 rpmG +0.4 0.8 50S ribosomal protein L33 (NCBI) compare
b2237 inaA +0.4 1.6 hypothetical protein (NCBI) compare
b3166 truB +0.4 3.2 tRNA pseudouridine synthase B (NCBI) compare
b4413 sokC +0.4 1.2 regulatory antisense RNA (NCBI) compare
b4593 ymgI +0.4 0.6 hypothetical protein (RefSeq) compare
b2634 yfjR +0.4 2.7 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b2051 wcaH +0.4 1.1 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0289 yagV +0.4 2.5 orf, hypothetical protein (VIMSS) compare
b4152 frdC +0.4 1.8 fumarate reductase subunit C (NCBI) compare
b1739 osmE +0.4 1.1 DNA-binding transcriptional activator (NCBI) compare
b2426 ucpA +0.4 1.5 putative oxidoreductase (VIMSS) compare
b0895 dmsB +0.4 1.6 dimethyl sulfoxide reductase, anaerobic, subunit B (NCBI) compare
b1689 b1689 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b3238 yhcN +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1628 rsxB +0.4 2.1 electron transport complex protein RnfB (NCBI) compare
b2811 ygdK +0.4 2.4 predicted Fe-S metabolism protein (NCBI) compare
b3228 sspB +0.4 2.2 ClpXP protease specificity-enhancing factor (NCBI) compare
b2684 mprA +0.4 1.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2248 yfaX +0.4 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1540 ydfH +0.4 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1194 ycgR +0.4 1.4 protein involved in flagellar function (NCBI) compare
b4436 ryeC +0.4 0.9 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1247 oppF +0.4 1.8 oligopeptide transporter subunit (NCBI) compare
b1953 b1953 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b4570 lomR +0.4 1.4 no description compare
b4695 ykgT +0.4 1.1 no description compare
b0364 yaiS +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b0426 yajQ +0.4 2.9 orf, hypothetical protein (VIMSS) compare
b0784 moaD +0.4 1.9 molybdopterin synthase, small subunit (NCBI) compare
b2457 cchA +0.4 1.1 detox protein (VIMSS) compare
b4493 gapC +0.4 2.0 no description compare
b2225 yfaP +0.4 2.9 hypothetical protein (NCBI) compare
b4519 icdC +0.4 1.6 no description compare
b4406 yaeP +0.4 1.7 hypothetical protein (NCBI) compare
b2061 wzb +0.4 1.4 protein-tyrosine phosphatase (NCBI) compare
b1620 malI +0.4 1.1 DNA-binding transcriptional repressor (NCBI) compare
b1493 gadB +0.4 0.5 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b2421 cysM +0.4 1.3 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b2904 gcvH +0.4 0.9 glycine cleavage system protein H (NCBI) compare
b1410 ynbC +0.4 2.4 predicted hydrolase (NCBI) compare
b2047 wcaJ +0.4 1.6 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b0487 cueR +0.4 1.6 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b0247 ykfG +0.4 0.8 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2281 nuoI +0.4 1.2 NADH dehydrogenase subunit I (NCBI) compare
b0535 fimZ +0.4 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1492 gadC +0.4 2.2 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1950 fliR +0.4 1.5 flagellar biosynthesis protein R (NCBI) compare
b2989 b2989 +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b4232 fbp +0.4 2.8 fructose-1,6-bisphosphatase (NCBI) compare
b1959 yedA +0.3 1.9 predicted inner membrane protein (NCBI) compare
b3443 yrhA +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1186 nhaB +0.3 2.6 NhaB sodium/proton transporter (Katherine Huang) compare
b2475 ypfJ +0.3 2.5 hypothetical protein (NCBI) compare
b4551 yheV +0.3 1.2 hypothetical protein (NCBI) compare
b1582 ynfA +0.3 1.6 hypothetical protein (NCBI) compare
b1729 ydjN +0.3 1.6 predicted transporter (NCBI) compare
b2093 gatB +0.3 1.5 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b1642 slyA +0.3 1.2 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2991 hybF +0.3 1.9 protein involved with the maturation of hydrogenases 1 and 2 (NCBI) compare
b2498 upp +0.3 1.3 uracil phosphoribosyltransferase (VIMSS) compare
b3085 ygjP +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b0502 ylbG +0.3 1.2 no description compare
b0404 yajB +0.3 1.4 hypothetical protein (NCBI) compare
b2534 yfhR +0.3 2.3 predicted peptidase (RefSeq) compare
b2280 nuoJ +0.3 2.0 NADH dehydrogenase subunit J (NCBI) compare
b3033 yqiB +0.3 1.3 predicted dehydrogenase (NCBI) compare
b2635 ypjK +0.3 1.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2673 nrdH +0.3 1.2 glutaredoxin-like protein (NCBI) compare
b2814 metZ +0.3 0.9 tRNA-Met (NCBI) compare
b1516 lsrB +0.3 1.6 AI2 transporter (NCBI) compare
b2157 yeiE +0.3 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3926 glpK +0.3 2.9 glycerol kinase (NCBI) compare
b1789 yeaL +0.3 1.3 conserved inner membrane protein (NCBI) compare
b1572 ydfB +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b2283 nuoG +0.3 2.2 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2439 eutL +0.3 1.5 predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI) compare
b1266 yciV +0.3 1.9 hypothetical protein (NCBI) compare
b3658 selC +0.3 1.3 tRNA-Sec (NCBI) compare
b0931 pncB +0.3 1.8 nicotinate phosphoribosyltransferase (NCBI) compare
b2141 yohJ +0.3 0.9 hypothetical protein (NCBI) compare
b3146 yraL +0.3 2.1 predicted methyltransferase (NCBI) compare
b0989 cspH +0.3 1.0 stress protein, member of the CspA-family (NCBI) compare
b2195 ccmG +0.3 1.6 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1243 oppA +0.3 2.1 oligopeptide transporter subunit (NCBI) compare
b0082 mraW +0.3 1.5 S-adenosyl-methyltransferase (NCBI) compare
b1669 ydhT +0.3 1.3 hypothetical protein (NCBI) compare
b0549 ybcO +0.3 1.4 DLP12 prophage; predicted protein (NCBI) compare
b4133 cadC +0.3 2.8 DNA-binding transcriptional activator (NCBI) compare
b0683 fur +0.3 0.6 ferric uptake regulator (NCBI) compare
b4196 ulaD +0.3 1.9 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI) compare
b4154 frdA +0.3 2.8 fumarate reductase (NCBI) compare
b2587 kgtP +0.3 1.9 alpha-ketoglutarate transporter (NCBI) compare
b1634 tppB +0.3 1.7 putative tripeptide transporter permease (NCBI) compare
b1298 ycjL +0.3 1.5 probable amidotransferase subunit (VIMSS) compare
b0357 yaiN +0.3 1.0 putative alpha helix chain (VIMSS) compare
b1842 holE +0.3 0.8 DNA polymerase III, theta subunit (NCBI) compare
b0013 yaaI +0.3 1.7 hypothetical protein (NCBI) compare
b1289 ycjD +0.3 1.8 hypothetical protein (NCBI) compare
b1388 paaA +0.3 1.8 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b2284 nuoF +0.3 1.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1144 ymfJ +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2946 yggJ +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b3495 uspA +0.3 2.4 universal stress global response regulator (NCBI) compare
b2939 yqgB +0.3 0.7 predicted protein (RefSeq) compare
b4522 yciX_1 +0.3 1.1 hypothetical protein (NCBI) compare
b1951 rcsA +0.3 0.6 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b3889 yiiE +0.3 0.9 predicted transcriptional regulator (RefSeq) compare
b1860 ruvB +0.3 0.2 Holliday junction DNA helicase B (NCBI) compare
b0450 glnK +0.3 1.3 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b1521 uxaB +0.3 2.0 tagaturonate reductase (NCBI) compare
b2095 gatZ +0.3 1.5 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b4623 insO +0.3 1.7 no description compare
b4222 ytfP +0.3 1.9 hypothetical protein (NCBI) compare
b1329 b1329 +0.3 1.9 putative transport periplasmic protein (VIMSS) compare
b0375 yaiV +0.3 1.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2366 dsdA +0.3 1.3 D-serine dehydratase (NCBI) compare
b1536 ydeI +0.3 0.4 hypothetical protein (NCBI) compare
b2502 ppx +0.3 2.2 exopolyphosphatase (NCBI) compare
b2959 yggL +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1246 oppD +0.3 2.0 oligopeptide transporter ATP-binding component (NCBI) compare
b1632 rsxE +0.3 1.9 NADH-ubiquinone oxidoreductase (NCBI) compare
b1535 ydeH +0.3 1.6 hypothetical protein (NCBI) compare
b2288 nuoA +0.3 0.9 NADH dehydrogenase I chain A (VIMSS) compare
b1692 ydiB +0.3 1.4 quinate/shikimate 5-dehydrogenase, NAD(P)-binding (NCBI) compare
b0696 kdpC +0.3 1.0 potassium-transporting ATPase subunit C (NCBI) compare
b1195 ymgE +0.3 1.1 predicted inner membrane protein (NCBI) compare
b2993 hybD +0.3 1.5 predicted maturation element for hydrogenase 2 (NCBI) compare
b4505 insX +0.3 0.5 predicted IS protein (RefSeq) compare
b4190 yjfP +0.3 1.8 predicted hydrolase (NCBI) compare
b1960 vsr +0.3 1.0 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1305 pspB +0.3 1.4 phage shock protein B (NCBI) compare
b4127 yjdJ +0.3 2.0 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2998 yghW +0.3 1.5 hypothetical protein (NCBI) compare
b1085 b1085 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1889 motB +0.3 1.7 flagellar motor protein MotB (NCBI) compare
b0714 nei +0.3 1.5 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1382 ynbE +0.3 0.7 predicted lipoprotein (NCBI) compare
b2286 nuoC +0.3 1.3 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1968 yedV +0.3 1.1 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b2250 b2250 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b0141 yadN +0.3 1.7 predicted fimbrial-like adhesin protein (NCBI) compare
b2637 yfjT +0.3 1.4 CP4-57 prophage; predicted protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress 5-Fluorodihydropyrimidine-2,4-dione in Escherichia coli BW25113

For stress 5-Fluorodihydropyrimidine-2,4-dione across organisms