Experiment set56IT069 for Escherichia coli BW25113

Compare to:

M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

200 most detrimental genes:

  gene name fitness t score description  
b2498 upp +15.4 42.2 uracil phosphoribosyltransferase (VIMSS) compare
b2297 pta +2.0 1.0 phosphate acetyltransferase (NCBI) compare
b1860 ruvB +2.0 1.0 Holliday junction DNA helicase B (NCBI) compare
b2055 wcaE +1.9 1.0 predicted glycosyl transferase (NCBI) compare
b1576 ydfD +1.9 0.9 Qin prophage; predicted protein (NCBI) compare
b4214 cysQ +1.9 16.4 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b1167 ymgC +1.9 0.9 hypothetical protein (NCBI) compare
b2352 yfdI +1.8 1.9 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b1001 yccE +1.8 0.9 hypothetical protein (NCBI) compare
b4443 gcvB +1.8 0.9 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b1257 yciE +1.8 0.9 hypothetical protein (NCBI) compare
b1499 ydeO +1.8 0.9 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1497 b1497 +1.8 0.9 putative enzyme (VIMSS) compare
b2830 nudH +1.8 0.9 dinucleoside polyphosphate hydrolase (NCBI) compare
b1712 ihfA +1.7 1.0 integration host factor subunit alpha (NCBI) compare
b1347 ydaC +1.7 0.8 Rac prophage; predicted protein (NCBI) compare
b2853 ygeI +1.7 0.8 hypothetical protein (NCBI) compare
b3121 yhaC +1.7 0.8 hypothetical protein (NCBI) compare
b2847 yqeI +1.7 1.3 predicted transcriptional regulator (NCBI) compare
b1130 phoP +1.7 1.7 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b2846 yqeH +1.7 1.2 orf, hypothetical protein (VIMSS) compare
b1551 b1551 +1.6 0.9 orf, hypothetical protein (VIMSS) compare
b1165 ymgA +1.6 1.0 hypothetical protein (NCBI) compare
b4651 yibW +1.6 0.8 no description compare
b2625 yfjI +1.6 3.4 CP4-57 prophage; predicted protein (NCBI) compare
b3442 yhhZ +1.6 0.8 hypothetical protein (NCBI) compare
b3979 thrT +1.6 0.9 tRNA-Thr (NCBI) compare
b3725 pstB +1.6 1.0 phosphate transporter subunit (NCBI) compare
b1361 ydaW +1.6 1.4 orf, hypothetical protein (VIMSS) compare
b4172 hfq +1.6 0.8 RNA-binding protein Hfq (NCBI) compare
b3463 ftsE +1.6 0.9 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1409 ynbB +1.6 1.4 predicted CDP-diglyceride synthase (NCBI) compare
b1363 trkG +1.6 1.0 Rac prophage; potassium transporter subunit (NCBI) compare
b3386 rpe +1.5 0.9 ribulose-phosphate 3-epimerase (NCBI) compare
b4593 ymgI +1.5 2.0 hypothetical protein (RefSeq) compare
b0658 ybeX +1.5 0.8 predicteed ion transport (NCBI) compare
b4279 yjhB +1.5 0.7 putative transport protein (VIMSS) compare
b0603 ybdO +1.5 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1182 hlyE +1.5 0.9 hemolysin E (VIMSS) compare
b2820 recB +1.5 1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b4660 yhiL +1.5 0.8 no description compare
b1690 ydiM +1.5 3.0 predicted transporter (NCBI) compare
b4155 yjeA +1.5 0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b3035 tolC +1.5 2.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3504 yhiS +1.5 0.8 hypothetical protein (RefSeq) compare
b1773 ydjI +1.5 3.4 predicted aldolase (NCBI) compare
b0500 ybbD +1.5 0.7 hypothetical protein (RefSeq) compare
b1351 racC +1.4 0.9 Rac prophage; predicted protein (NCBI) compare
b1865 nudB +1.4 2.1 dATP pyrophosphohydrolase (NCBI) compare
b1951 rcsA +1.4 2.0 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b3405 ompR +1.4 0.9 osmolarity response regulator (NCBI) compare
b2652 ileY +1.4 0.9 tRNA-Ile (NCBI) compare
b4128 yjdK +1.4 0.8 hypothetical protein (NCBI) compare
b1129 phoQ +1.4 0.9 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b3443 yrhA +1.4 1.8 orf, hypothetical protein (VIMSS) compare
b4462 ygaQ +1.4 2.0 hypothetical protein (RefSeq) compare
b4497 yeeL +1.4 1.3 no description compare
b0738 tolR +1.4 0.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0118 acnB +1.4 0.8 aconitate hydratase (NCBI) compare
b2894 xerD +1.4 0.8 site-specific tyrosine recombinase XerD (NCBI) compare
b1536 ydeI +1.4 1.2 hypothetical protein (NCBI) compare
b0705 ybfL +1.3 0.8 putative receptor protein (VIMSS) compare
b4701 sokX +1.3 1.3 sok-related sRNA, function unknown (RefSeq) compare
b1863 ruvC +1.3 1.4 Holliday junction resolvase (NCBI) compare
b2531 iscR +1.3 2.1 DNA-binding transcriptional repressor (NCBI) compare
b4177 purA +1.3 0.8 adenylosuccinate synthetase (NCBI) compare
b3261 fis +1.3 2.8 DNA-binding protein Fis (NCBI) compare
b3875 ompL +1.3 3.6 predicted outer membrane porin L (NCBI) compare
b3290 trkA +1.3 1.2 potassium transporter peripheral membrane component (NCBI) compare
b4066 yjcF +1.3 0.8 hypothetical protein (NCBI) compare
b0279 yagM +1.3 1.1 CP4-6 prophage; predicted protein (NCBI) compare
b4494 arpB +1.3 2.4 no description compare
b1861 ruvA +1.3 0.8 Holliday junction DNA helicase motor protein (NCBI) compare
b0890 ftsK +1.2 3.1 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0114 aceE +1.2 1.1 pyruvate dehydrogenase subunit E1 (NCBI) compare
b3026 qseC +1.2 0.8 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3889 yiiE +1.2 3.5 predicted transcriptional regulator (RefSeq) compare
b1159 mcrA +1.2 1.1 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b1762 b1762 +1.2 2.9 orf, hypothetical protein (VIMSS) compare
b4590 ybfK +1.2 1.1 hypothetical protein (RefSeq) compare
b0740 tolB +1.2 0.8 translocation protein TolB precursor (NCBI) compare
b2215 ompC +1.2 0.8 outer membrane porin protein C (NCBI) compare
b0737 tolQ +1.2 0.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4215 ytfI +1.2 2.6 hypothetical protein (NCBI) compare
b1236 galU +1.1 2.5 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0303 ykgI +1.1 1.0 orf, hypothetical protein (VIMSS) compare
b0739 tolA +1.1 0.7 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2168 fruK +1.1 1.4 1-phosphofructokinase (NCBI) compare
b3183 obgE +1.1 1.6 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4042 dgkA +1.1 0.7 diacylglycerol kinase (NCBI) compare
b2110 yehC +1.1 1.2 predicted periplasmic pilin chaperone (NCBI) compare
b3811 xerC +1.1 0.7 site-specific tyrosine recombinase XerC (NCBI) compare
b3863 polA +1.1 0.7 DNA polymerase I (NCBI) compare
b2741 rpoS +1.1 5.8 RNA polymerase sigma factor (NCBI) compare
b2650 ypjC +1.0 0.4 hypothetical protein (RefSeq) compare
b1739 osmE +1.0 2.3 DNA-binding transcriptional activator (NCBI) compare
b1527 yneK +1.0 3.3 hypothetical protein (NCBI) compare
b1505 ydeT +1.0 1.2 hypothetical protein (RefSeq) compare
b1579 intQ +1.0 2.7 Qin prophage; predicted defective integrase (RefSeq) compare
b1293 sapB +1.0 1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1709 btuD +1.0 1.4 vitamin B12-transporter ATPase (NCBI) compare
b1545 pinQ +1.0 0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b0741 pal +1.0 0.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4239 treC +0.9 0.6 trehalose-6-P hydrolase (NCBI) compare
b4640 yoeG +0.9 2.6 no description compare
b2375 yfdX +0.9 3.6 hypothetical protein (NCBI) compare
b1258 yciF +0.9 0.6 hypothetical protein (NCBI) compare
b0326 yahL +0.9 2.3 hypothetical protein (NCBI) compare
b1121 ycfZ +0.9 1.8 predicted inner membrane protein (NCBI) compare
b3120 yhaB +0.9 1.8 orf, hypothetical protein (VIMSS) compare
b1730 ydjO +0.9 1.1 predicted protein (RefSeq) compare
b1400 paaY +0.9 2.5 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2419 yfeK +0.9 2.1 hypothetical protein (NCBI) compare
b1874 cutC +0.9 0.6 copper homeostasis protein (NCBI) compare
b1309 ycjM +0.9 4.1 putative polysaccharide hydrolase (VIMSS) compare
b2859 ygeO +0.9 1.6 hypothetical protein (RefSeq) compare
b4253 yjgL +0.9 3.4 orf, hypothetical protein (VIMSS) compare
b4509 ylcG +0.9 3.1 DLP12 prophage; predicted protein (NCBI) compare
b2369 evgA +0.9 2.7 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b3622 rfaL +0.9 2.3 O-antigen ligase (NCBI) compare
b4559 yjdO +0.9 3.5 hypothetical protein (NCBI) compare
b1040 csgD +0.9 1.5 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b0310 ykgH +0.9 1.1 predicted inner membrane protein (NCBI) compare
b0530 sfmA +0.9 3.3 putative fimbrial-like protein (VIMSS) compare
b1658 purR +0.9 4.1 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1735 chbR +0.9 2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b1292 sapC +0.9 1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4568 ytjA +0.8 2.6 predicted protein (RefSeq) compare
b0218 yafU +0.8 2.8 predicted inner membrane protein (RefSeq) compare
b4650 yibS +0.8 2.3 no description compare
b1691 ydiN +0.8 2.9 predicted transporter (RefSeq) compare
b0990 cspG +0.8 1.1 DNA-binding transcriptional regulator (NCBI) compare
b1502 ydeQ +0.8 2.2 predicted fimbrial-like adhesin protein (NCBI) compare
b2904 gcvH +0.8 2.0 glycine cleavage system protein H (NCBI) compare
b1305 pspB +0.8 2.8 phage shock protein B (NCBI) compare
b0234 yafP +0.8 1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1785 yeaI +0.8 2.4 predicted diguanylate cyclase (NCBI) compare
b2734 pphB +0.8 3.5 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b1346 ydaQ +0.8 1.3 orf, hypothetical protein (VIMSS) compare
b1238 tdk +0.8 4.2 thymidine kinase (NCBI) compare
b2163 yeiL +0.8 3.7 putative transcriptional regulator (VIMSS) compare
b2282 nuoH +0.8 2.2 NADH dehydrogenase subunit H (NCBI) compare
b1968 yedV +0.8 2.1 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b1969 yedW +0.7 3.2 putative 2-component transcriptional regulator (VIMSS) compare
b1784 yeaH +0.7 4.2 yeaH component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b0706 ybfD +0.7 1.8 hypothetical protein (NCBI) compare
b4345 mcrC +0.7 0.7 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1583 ynfB +0.7 3.0 hypothetical protein (NCBI) compare
b3723 bglG +0.7 3.3 transcriptional antiterminator of the bgl operon (NCBI) compare
b3359 argD +0.7 6.1 bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase (NCBI) compare
b0798 ybiA +0.7 2.8 hypothetical protein (NCBI) compare
b1480 sra +0.7 1.0 30S ribosomal subunit protein S22 (NCBI) compare
b1689 b1689 +0.7 2.6 orf, hypothetical protein (VIMSS) compare
b0989 cspH +0.7 2.1 stress protein, member of the CspA-family (NCBI) compare
b2283 nuoG +0.7 4.5 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b3483 yhhH +0.7 0.3 orf, hypothetical protein (VIMSS) compare
b1223 narK +0.7 0.8 nitrate/nitrite transporter (NCBI) compare
b4704 arrS +0.7 0.3 Antisense sRNA ArrS, function unknown (NCBI) compare
b4675 yoaJ +0.7 1.5 expressed protein (RefSeq) compare
b0136 yadK +0.7 2.6 predicted fimbrial-like adhesin protein (NCBI) compare
b1333 uspE +0.7 1.7 stress-induced protein (NCBI) compare
b2372 yfdV +0.7 4.2 predicted transporter (NCBI) compare
b1139 lit +0.7 3.4 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b0784 moaD +0.7 3.4 molybdopterin synthase, small subunit (NCBI) compare
b0141 yadN +0.7 4.5 predicted fimbrial-like adhesin protein (NCBI) compare
b2659 b2659 +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b3625 rfaY +0.7 3.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1642 slyA +0.7 2.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3462 ftsX +0.7 3.0 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3778 rep +0.7 3.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3613 yibP +0.7 0.8 putative membrane protein (VIMSS) compare
b3068 ygjF +0.7 2.5 G/U mismatch-specific DNA glycosylase (NCBI) compare
b4679 yohP +0.7 1.9 expressed protein (RefSeq) compare
b4299 yjhI +0.7 4.2 KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI) compare
b2236 yfaE +0.7 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3181 greA +0.7 3.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3341 rpsG +0.7 1.1 30S ribosomal protein S7 (NCBI) compare
b4170 mutL +0.6 2.3 DNA mismatch repair protein (NCBI) compare
b1950 fliR +0.6 1.9 flagellar biosynthesis protein R (NCBI) compare
b0912 ihfB +0.6 0.4 integration host factor subunit beta (NCBI) compare
b0531 sfmC +0.6 3.4 pilin chaperone, periplasmic (NCBI) compare
b1882 cheY +0.6 0.4 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1877 yecT +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +0.6 0.4 predicted inner membrane protein (NCBI) compare
b2143 cdd +0.6 2.8 cytidine deaminase (NCBI) compare
b2367 emrY +0.6 3.3 predicted multidrug efflux system (NCBI) compare
b0675 nagD +0.6 4.6 UMP phosphatase (NCBI) compare
b0247 ykfG +0.6 1.2 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0535 fimZ +0.6 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3027 ygiZ +0.6 0.4 conserved inner membrane protein (NCBI) compare
b1581 rspA +0.6 2.3 predicted dehydratase (NCBI) compare
b1707 ydiV +0.6 2.5 hypothetical protein (NCBI) compare
b4418 sraB +0.6 1.1 unknown RNA (NCBI) compare
b2935 tktA +0.6 1.1 transketolase 1, thiamin-binding (NCBI) compare
b4631 ykgQ +0.6 1.2 no description compare
b0787 ybhM +0.6 2.8 conserved inner membrane protein (NCBI) compare
b0508 hyi +0.6 2.1 hydroxypyruvate isomerase (NCBI) compare
b4438 cyaR +0.6 1.4 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b3781 trxA +0.6 2.7 thioredoxin 1 (VIMSS) compare
b2279 nuoK +0.6 1.0 NADH dehydrogenase subunit K (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress 5'-Deoxy-5-fluorocytidine in Escherichia coli BW25113

For stress 5'-Deoxy-5-fluorocytidine across organisms