Experiment set56IT068 for Escherichia coli BW25113

Compare to:

M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

200 most detrimental genes:

  gene name fitness t score description  
b2498 upp +15.5 47.9 uracil phosphoribosyltransferase (VIMSS) compare
b1257 yciE +2.1 1.2 hypothetical protein (NCBI) compare
b4128 yjdK +2.0 2.0 hypothetical protein (NCBI) compare
b2297 pta +1.9 0.9 phosphate acetyltransferase (NCBI) compare
b1860 ruvB +1.9 0.9 Holliday junction DNA helicase B (NCBI) compare
b2055 wcaE +1.9 0.9 predicted glycosyl transferase (NCBI) compare
b4214 cysQ +1.8 16.1 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b1167 ymgC +1.8 0.9 hypothetical protein (NCBI) compare
b1576 ydfD +1.8 0.9 Qin prophage; predicted protein (NCBI) compare
b1865 nudB +1.7 3.2 dATP pyrophosphohydrolase (NCBI) compare
b1001 yccE +1.7 0.8 hypothetical protein (NCBI) compare
b4443 gcvB +1.7 0.8 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b1499 ydeO +1.7 0.8 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1497 b1497 +1.7 0.8 putative enzyme (VIMSS) compare
b2830 nudH +1.7 0.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b1712 ihfA +1.6 1.0 integration host factor subunit alpha (NCBI) compare
b1363 trkG +1.6 1.5 Rac prophage; potassium transporter subunit (NCBI) compare
b2853 ygeI +1.6 0.8 hypothetical protein (NCBI) compare
b1347 ydaC +1.6 0.8 Rac prophage; predicted protein (NCBI) compare
b2352 yfdI +1.6 1.4 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b3121 yhaC +1.6 0.8 hypothetical protein (NCBI) compare
b2894 xerD +1.6 1.2 site-specific tyrosine recombinase XerD (NCBI) compare
b2847 yqeI +1.6 0.9 predicted transcriptional regulator (NCBI) compare
b1165 ymgA +1.6 0.9 hypothetical protein (NCBI) compare
b4651 yibW +1.5 0.8 no description compare
b3442 yhhZ +1.5 0.7 hypothetical protein (NCBI) compare
b1130 phoP +1.5 1.0 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b1551 b1551 +1.5 0.9 orf, hypothetical protein (VIMSS) compare
b4647 mokA +1.5 2.3 no description compare
b1861 ruvA +1.5 1.3 Holliday junction DNA helicase motor protein (NCBI) compare
b3725 pstB +1.5 0.9 phosphate transporter subunit (NCBI) compare
b3463 ftsE +1.5 0.8 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4172 hfq +1.5 0.7 RNA-binding protein Hfq (NCBI) compare
b3386 rpe +1.5 0.9 ribulose-phosphate 3-epimerase (NCBI) compare
b1258 yciF +1.4 1.7 hypothetical protein (NCBI) compare
b1182 hlyE +1.4 0.9 hemolysin E (VIMSS) compare
b4215 ytfI +1.4 3.4 hypothetical protein (NCBI) compare
b1690 ydiM +1.4 2.9 predicted transporter (NCBI) compare
b0658 ybeX +1.4 0.7 predicteed ion transport (NCBI) compare
b1409 ynbB +1.4 0.9 predicted CDP-diglyceride synthase (NCBI) compare
b4279 yjhB +1.4 0.7 putative transport protein (VIMSS) compare
b2820 recB +1.4 0.9 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0603 ybdO +1.4 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1773 ydjI +1.4 2.7 predicted aldolase (NCBI) compare
b1332 ynaJ +1.4 0.9 predicted inner membrane protein (NCBI) compare
b4660 yhiL +1.4 0.8 no description compare
b4155 yjeA +1.4 0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b1730 ydjO +1.4 2.0 predicted protein (RefSeq) compare
b1351 racC +1.4 0.8 Rac prophage; predicted protein (NCBI) compare
b2625 yfjI +1.4 2.4 CP4-57 prophage; predicted protein (NCBI) compare
b1429 tehA +1.4 2.2 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b0737 tolQ +1.4 1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0500 ybbD +1.4 0.7 hypothetical protein (RefSeq) compare
b2110 yehC +1.4 2.2 predicted periplasmic pilin chaperone (NCBI) compare
b3504 yhiS +1.4 0.8 hypothetical protein (RefSeq) compare
b0683 fur +1.4 1.8 ferric uptake regulator (NCBI) compare
b1129 phoQ +1.3 0.9 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b2652 ileY +1.3 0.9 tRNA-Ile (NCBI) compare
b2363 yfdT +1.3 4.6 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b0118 acnB +1.3 0.8 aconitate hydratase (NCBI) compare
b3405 ompR +1.3 0.9 osmolarity response regulator (NCBI) compare
b3035 tolC +1.3 0.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0738 tolR +1.3 0.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4497 yeeL +1.3 0.9 no description compare
b2279 nuoK +1.3 2.5 NADH dehydrogenase subunit K (NCBI) compare
b4590 ybfK +1.3 1.5 hypothetical protein (RefSeq) compare
b4462 ygaQ +1.3 1.5 hypothetical protein (RefSeq) compare
b3026 qseC +1.3 1.6 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0705 ybfL +1.3 0.8 putative receptor protein (VIMSS) compare
b4640 yoeG +1.2 4.0 no description compare
b1506 yneL +1.2 2.8 predicted transcriptional regulator (NCBI) compare
b4177 purA +1.2 0.8 adenylosuccinate synthetase (NCBI) compare
b1291 sapD +1.2 1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4066 yjcF +1.2 0.7 hypothetical protein (NCBI) compare
b3341 rpsG +1.2 2.3 30S ribosomal protein S7 (NCBI) compare
b1159 mcrA +1.2 1.1 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b1968 yedV +1.2 3.7 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b1536 ydeI +1.2 0.8 hypothetical protein (NCBI) compare
b0279 yagM +1.2 0.7 CP4-6 prophage; predicted protein (NCBI) compare
b3290 trkA +1.1 0.8 potassium transporter peripheral membrane component (NCBI) compare
b2846 yqeH +1.1 0.7 orf, hypothetical protein (VIMSS) compare
b0740 tolB +1.1 0.8 translocation protein TolB precursor (NCBI) compare
b2831 mutH +1.1 1.4 DNA mismatch repair protein (NCBI) compare
b0114 aceE +1.1 0.7 pyruvate dehydrogenase subunit E1 (NCBI) compare
b2215 ompC +1.1 0.8 outer membrane porin protein C (NCBI) compare
b0890 ftsK +1.1 2.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1951 rcsA +1.1 1.2 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2642 yfjW +1.1 1.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1579 intQ +1.1 3.1 Qin prophage; predicted defective integrase (RefSeq) compare
b0739 tolA +1.1 0.7 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3889 yiiE +1.1 3.2 predicted transcriptional regulator (RefSeq) compare
b1874 cutC +1.1 1.0 copper homeostasis protein (NCBI) compare
b3875 ompL +1.0 2.4 predicted outer membrane porin L (NCBI) compare
b4042 dgkA +1.0 0.7 diacylglycerol kinase (NCBI) compare
b0741 pal +1.0 1.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2650 ypjC +1.0 0.4 hypothetical protein (RefSeq) compare
b1236 galU +1.0 1.9 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3811 xerC +1.0 0.7 site-specific tyrosine recombinase XerC (NCBI) compare
b1877 yecT +1.0 2.1 orf, hypothetical protein (VIMSS) compare
b1823 cspC +1.0 2.8 stress protein, member of the CspA-family (NCBI) compare
b4494 arpB +1.0 1.3 no description compare
b3863 polA +1.0 0.6 DNA polymerase I (NCBI) compare
b1505 ydeT +1.0 0.6 hypothetical protein (RefSeq) compare
b0326 yahL +0.9 2.4 hypothetical protein (NCBI) compare
b1368 ynaA +0.9 2.5 Rac prophage; predicted tail protein (RefSeq) compare
b4253 yjgL +0.9 4.0 orf, hypothetical protein (VIMSS) compare
b2939 yqgB +0.9 1.0 predicted protein (RefSeq) compare
b4017 arpA +0.9 3.7 regulator of acetyl CoA synthetase (NCBI) compare
b1762 b1762 +0.9 2.2 orf, hypothetical protein (VIMSS) compare
b1400 paaY +0.9 3.3 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b0303 ykgI +0.9 0.6 orf, hypothetical protein (VIMSS) compare
b1785 yeaI +0.9 2.6 predicted diguanylate cyclase (NCBI) compare
b1545 pinQ +0.9 0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3622 rfaL +0.9 2.4 O-antigen ligase (NCBI) compare
b4239 treC +0.9 0.8 trehalose-6-P hydrolase (NCBI) compare
b1739 osmE +0.9 3.2 DNA-binding transcriptional activator (NCBI) compare
b1600 mdtJ +0.9 1.8 multidrug efflux system transporter (NCBI) compare
b3613 yibP +0.9 1.3 putative membrane protein (VIMSS) compare
b1121 ycfZ +0.9 1.7 predicted inner membrane protein (NCBI) compare
b3462 ftsX +0.9 4.1 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0234 yafP +0.8 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4048 yjbM +0.8 1.4 hypothetical protein (NCBI) compare
b2369 evgA +0.8 2.6 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b0437 clpP +0.8 1.3 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2528 iscA +0.8 1.0 iron-sulfur cluster assembly protein (NCBI) compare
b0310 ykgH +0.8 0.9 predicted inner membrane protein (NCBI) compare
b4458 oxyS +0.8 1.6 global regulatory RNA (NCBI) compare
b1778 yeaA +0.8 1.9 methionine sulfoxide reductase B (NCBI) compare
b1850 eda +0.8 1.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1498 b1498 +0.8 4.8 putative sulfatase (VIMSS) compare
b0294 ykgK +0.8 3.3 predicted regulator (NCBI) compare
b3443 yrhA +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b3706 trmE +0.8 2.8 tRNA modification GTPase (NCBI) compare
b1465 narV +0.8 1.0 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b3624 rfaZ +0.8 1.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3723 bglG +0.8 3.5 transcriptional antiterminator of the bgl operon (NCBI) compare
b3359 argD +0.8 6.8 bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase (NCBI) compare
b1223 narK +0.8 1.1 nitrate/nitrite transporter (NCBI) compare
b3778 rep +0.7 4.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1524 yneH +0.7 1.1 predicted glutaminase (NCBI) compare
b3120 yhaB +0.7 1.3 orf, hypothetical protein (VIMSS) compare
b3146 yraL +0.7 5.3 predicted methyltransferase (NCBI) compare
b3658 selC +0.7 1.5 tRNA-Sec (NCBI) compare
b2911 ssrS +0.7 1.4 6S regulatory RNA (NCBI) compare
b1156 tfaE +0.7 3.4 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b1691 ydiN +0.7 2.2 predicted transporter (RefSeq) compare
b2946 yggJ +0.7 3.8 orf, hypothetical protein (VIMSS) compare
b3512 GadE +0.7 2.5 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4650 yibS +0.7 1.7 no description compare
b1168 b1168 +0.7 2.7 putative proteases (VIMSS) compare
b1658 purR +0.7 4.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1150 ymfR +0.7 1.1 e14 prophage; predicted protein (NCBI) compare
b2741 rpoS +0.7 3.2 RNA polymerase sigma factor (NCBI) compare
b4699 fnrS +0.7 0.8 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1557 cspB +0.7 1.8 Qin prophage; cold shock protein (NCBI) compare
b4589 ylcI +0.7 1.5 hypothetical protein (RefSeq) compare
b4552 yrhC +0.7 0.9 no description compare
b2318 truA +0.7 1.2 tRNA pseudouridine synthase A (NCBI) compare
b1476 fdnI +0.7 0.9 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1969 yedW +0.7 2.8 putative 2-component transcriptional regulator (VIMSS) compare
b0675 nagD +0.7 4.4 UMP phosphatase (NCBI) compare
b2287 nuoB +0.7 2.2 NADH dehydrogenase subunit B (NCBI) compare
b4345 mcrC +0.7 0.6 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b4299 yjhI +0.7 3.9 KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI) compare
b4704 arrS +0.7 0.3 Antisense sRNA ArrS, function unknown (NCBI) compare
b3483 yhhH +0.7 0.3 orf, hypothetical protein (VIMSS) compare
b2734 pphB +0.7 2.8 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b1348 lar +0.7 1.5 Rac prophage; restriction alleviation protein (NCBI) compare
b2236 yfaE +0.7 2.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1292 sapC +0.7 1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2163 yeiL +0.6 2.9 putative transcriptional regulator (VIMSS) compare
b1863 ruvC +0.6 0.6 Holliday junction resolvase (NCBI) compare
b0018 mokC +0.6 1.3 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2895 fldB +0.6 1.6 flavodoxin 2 (NCBI) compare
b2285 nuoE +0.6 0.9 NADH dehydrogenase subunit E (NCBI) compare
b4701 sokX +0.6 0.7 sok-related sRNA, function unknown (RefSeq) compare
b1040 csgD +0.6 0.7 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b3138 agaB +0.6 1.8 N-acetylgalactosamine-specific enzyme IIB component of PTS (NCBI) compare
b0828 iaaA +0.6 2.3 L-asparaginase (NCBI) compare
b0136 yadK +0.6 2.1 predicted fimbrial-like adhesin protein (NCBI) compare
b4559 yjdO +0.6 2.5 hypothetical protein (NCBI) compare
b0912 ihfB +0.6 0.4 integration host factor subunit beta (NCBI) compare
b1161 ycgX +0.6 0.6 hypothetical protein (NCBI) compare
b2504 yfgG +0.6 0.8 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b4325 yjiC +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b1780 yeaD +0.6 2.9 orf, hypothetical protein (VIMSS) compare
b3779 gpp +0.6 5.2 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b3183 obgE +0.6 0.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2471 yffB +0.6 2.7 hypothetical protein (NCBI) compare
b1542 ydfI +0.6 2.9 predicted mannonate dehydrogenase (NCBI) compare
b1824 yobF +0.6 1.7 hypothetical protein (NCBI) compare
b1139 lit +0.6 2.6 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b1818 manY +0.6 1.3 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0998 torD +0.6 1.0 chaperone protein TorD (NCBI) compare
b2367 emrY +0.6 3.1 predicted multidrug efflux system (NCBI) compare
b1157 stfE +0.6 2.6 no description compare
b0247 ykfG +0.6 1.1 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1058 yceO +0.6 0.3 hypothetical protein (NCBI) compare
b2935 tktA +0.6 1.0 transketolase 1, thiamin-binding (NCBI) compare
b1493 gadB +0.6 0.6 glutamate decarboxylase B, PLP-dependent (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress 5'-Deoxy-5-fluorocytidine in Escherichia coli BW25113

For stress 5'-Deoxy-5-fluorocytidine across organisms