Experiment set56IT068 for Escherichia coli BW25113

M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

Group: stress
Media: M9 + 5'-Deoxy-5-fluorocytidine (500 uM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, microplate, Aerobic, at 37 (C)
By: Ben Guthrie on 2/15/22
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 3 genes in this experiment

For stress 5'-Deoxy-5-fluorocytidine in Escherichia coli BW25113

For stress 5'-Deoxy-5-fluorocytidine across organisms

SEED Subsystems

Subsystem	#Specific
Capsular heptose biosynthesis	1
De Novo Pyrimidine Synthesis	1
Pyrimidine utilization	1
Rhamnose containing glycans	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Pyrimidine metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NAD salvage pathway III (to nicotinamide riboside)	3	3	1
UTP and CTP dephosphorylation I	7	5	2
purine nucleotides degradation II (aerobic)	11	11	3
inosine 5'-phosphate degradation	4	4	1
guanosine nucleotides degradation II	4	4	1
guanosine nucleotides degradation III	4	4	1
adenosine nucleotides degradation I	8	7	2
purine nucleotides degradation I (plants)	12	10	3
guanosine nucleotides degradation I	4	3	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
adenosine nucleotides degradation II	5	5	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
superpathway of guanosine nucleotides degradation (plants)	6	5	1
superpathway of purines degradation in plants	18	13	3
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
ureide biosynthesis	7	5	1
tunicamycin biosynthesis	9	2	1
O-antigen building blocks biosynthesis (E. coli)	11	11	1
NAD salvage (plants)	11	5	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	7	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	14	1