Experiment set56IT037 for Escherichia coli BW25113

M9 anaerobic no stress control

Group: m9 anaerobic no stress control
Media: M9 + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, microplate, Anaerobic, at 37 (C)
By: Ben Guthrie on 2/8/22
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 9 genes in this experiment

For m9 anaerobic no stress control Dimethyl Sulfoxide in Escherichia coli BW25113

For m9 anaerobic no stress control Dimethyl Sulfoxide across organisms

SEED Subsystems

Subsystem	#Specific
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
spermine and spermidine degradation I	5	2	2
putrescine degradation III	4	2	1