Experiment set56IT036 for Escherichia coli BW25113

Compare to:

M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

200 most detrimental genes:

  gene name fitness t score description  
b1882 cheY +1.7 0.8 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3183 obgE +1.7 2.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2285 nuoE +1.5 4.4 NADH dehydrogenase subunit E (NCBI) compare
b3636 rpmG +1.4 3.6 50S ribosomal protein L33 (NCBI) compare
b2287 nuoB +1.4 5.9 NADH dehydrogenase subunit B (NCBI) compare
b1480 sra +1.2 1.5 30S ribosomal subunit protein S22 (NCBI) compare
b2281 nuoI +1.1 4.4 NADH dehydrogenase subunit I (NCBI) compare
b4603 rseX +1.1 1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2502 ppx +1.1 7.9 exopolyphosphatase (NCBI) compare
b3779 gpp +1.1 9.5 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b2277 nuoM +1.0 7.1 NADH dehydrogenase subunit M (NCBI) compare
b3745 yieM +1.0 9.8 predicted von Willibrand factor containing protein (NCBI) compare
b2906 visC +1.0 6.6 hypothetical protein (NCBI) compare
b0955 ycbZ +1.0 7.7 predicted peptidase (NCBI) compare
b2498 upp +1.0 3.7 uracil phosphoribosyltransferase (VIMSS) compare
b2280 nuoJ +0.9 5.8 NADH dehydrogenase subunit J (NCBI) compare
b3746 yieN +0.9 8.6 putative 2-component regulator (VIMSS) compare
b3495 uspA +0.9 8.0 universal stress global response regulator (NCBI) compare
b1573 ydfC +0.9 1.2 Qin prophage; predicted protein (RefSeq) compare
b4232 fbp +0.9 7.2 fructose-1,6-bisphosphatase (NCBI) compare
b2278 nuoL +0.9 6.3 NADH dehydrogenase subunit L (NCBI) compare
b2283 nuoG +0.8 5.4 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b0191 yaeJ +0.8 4.8 hypothetical protein (NCBI) compare
b3512 GadE +0.8 3.8 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0401 brnQ +0.8 6.1 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b3390 aroK +0.8 1.1 shikimate kinase I (NCBI) compare
b2276 nuoN +0.8 4.3 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b2217 rcsB +0.8 6.1 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1658 purR +0.8 4.4 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0104 guaC +0.8 6.3 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b0232 yafN +0.8 0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1493 gadB +0.7 0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4154 frdA +0.7 6.5 fumarate reductase (NCBI) compare
b4153 frdB +0.7 5.5 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b2421 cysM +0.7 3.6 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b2284 nuoF +0.7 2.9 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1286 rnb +0.7 1.7 exoribonuclease II (NCBI) compare
b2286 nuoC +0.7 3.5 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b4151 frdD +0.7 2.3 fumarate reductase subunit D (NCBI) compare
b1583 ynfB +0.7 1.9 hypothetical protein (NCBI) compare
b2497 uraA +0.7 5.5 uracil transporter (NCBI) compare
b1492 gadC +0.7 3.7 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b4152 frdC +0.7 2.9 fumarate reductase subunit C (NCBI) compare
b1764 selD +0.7 3.3 selenophosphate synthetase (NCBI) compare
b2939 yqgB +0.6 1.5 predicted protein (RefSeq) compare
b1161 ycgX +0.6 0.7 hypothetical protein (NCBI) compare
b0827 moeA +0.6 4.0 molybdopterin biosynthesis protein (NCBI) compare
b1553 ydfP +0.6 2.1 Qin prophage; conserved protein (NCBI) compare
b3895 fdhD +0.6 4.5 formate dehydrogenase accessory protein (NCBI) compare
b4222 ytfP +0.6 3.6 hypothetical protein (NCBI) compare
b3778 rep +0.6 3.9 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4414 tff +0.6 1.6 unknown RNA (NCBI) compare
b1007 b1007 +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b4695 ykgT +0.6 1.9 no description compare
b4064 yjcD +0.6 4.3 predicted permease (NCBI) compare
b1186 nhaB +0.6 4.2 NhaB sodium/proton transporter (Katherine Huang) compare
b1070 flgN +0.6 1.3 export chaperone for FlgK and FlgL (NCBI) compare
b3002 yqhA +0.6 1.8 hypothetical protein (NCBI) compare
b1343 dbpA +0.6 3.8 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b2282 nuoH +0.6 2.4 NADH dehydrogenase subunit H (NCBI) compare
b1246 oppD +0.6 4.3 oligopeptide transporter ATP-binding component (NCBI) compare
b1153 ymfQ +0.6 2.3 e14 prophage; conserved protein (NCBI) compare
b3590 selB +0.6 5.1 selenocysteinyl-tRNA-specific translation factor (NCBI) compare
b2096 gatY +0.6 4.0 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4369 leuP +0.6 2.5 tRNA-Leu (NCBI) compare
b3042 b3042 +0.6 3.2 orf, hypothetical protein (VIMSS) compare
b1548 nohA +0.6 1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b2948 yqgE +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b2480 bcp +0.5 3.6 thioredoxin-dependent thiol peroxidase (NCBI) compare
b0108 ppdD +0.5 2.2 predicted major pilin subunit (NCBI) compare
b2688 gshA +0.5 3.7 glutamate--cysteine ligase (NCBI) compare
b3816 corA +0.5 4.1 magnesium/nickel/cobalt transporter (NCBI) compare
b0219 yafV +0.5 1.9 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b2814 metZ +0.5 1.2 tRNA-Met (NCBI) compare
b4408 csrB +0.5 2.8 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1524 yneH +0.5 0.6 predicted glutaminase (NCBI) compare
b3166 truB +0.5 3.5 tRNA pseudouridine synthase B (NCBI) compare
b1519 tam +0.5 2.4 trans-aconitate 2-methyltransferase (NCBI) compare
b1696 ydiP +0.5 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3236 mdh +0.5 3.1 malate dehydrogenase (NCBI) compare
b1454 yncG +0.5 1.4 predicted enzyme (NCBI) compare
b3206 npr +0.5 3.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2236 yfaE +0.5 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2168 fruK +0.5 0.7 1-phosphofructokinase (NCBI) compare
b3892 fdoI +0.5 3.8 formate dehydrogenase-O, cytochrome b556 subunit (NCBI) compare
b2279 nuoK +0.5 0.6 NADH dehydrogenase subunit K (NCBI) compare
b0790 ybhP +0.5 2.9 predicted DNase (NCBI) compare
b1038 csgF +0.5 1.1 predicted transport protein (NCBI) compare
b0167 glnD +0.5 2.3 PII uridylyl-transferase (NCBI) compare
b3181 greA +0.5 2.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1187 fadR +0.5 2.0 fatty acid metabolism regulator (NCBI) compare
b3591 selA +0.5 4.3 selenocysteine synthase (NCBI) compare
b3537 yhjT +0.5 2.7 orf, hypothetical protein (VIMSS) compare
b0357 yaiN +0.5 0.9 putative alpha helix chain (VIMSS) compare
b2346 vacJ +0.5 3.1 predicted lipoprotein (NCBI) compare
b1143 ymfI +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1582 ynfA +0.5 1.8 hypothetical protein (NCBI) compare
b1428 ydcK +0.5 3.1 predicted enzyme (NCBI) compare
b2528 iscA +0.5 1.2 iron-sulfur cluster assembly protein (NCBI) compare
b4409 blr +0.5 3.4 beta-lactam resistance membrane protein (NCBI) compare
b1607 ydgC +0.5 0.9 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b0934 ycbM +0.5 1.1 putative transport system permease protein (VIMSS) compare
b4416 rybA +0.5 1.1 unknown RNA (NCBI) compare
b2946 yggJ +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b2047 wcaJ +0.5 2.6 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b0435 bolA +0.5 3.1 possible regulator of murein genes (VIMSS) compare
b2664 ygaE +0.5 2.7 putative transcriptional regulator (VIMSS) compare
b4431 rprA +0.5 1.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b4521 ycgI +0.5 2.2 hypothetical protein (NCBI) compare
b1789 yeaL +0.5 1.8 conserved inner membrane protein (NCBI) compare
b3884 yihW +0.5 2.4 putative DEOR-type transcriptional regulator (VIMSS) compare
b1307 pspD +0.5 1.3 peripheral inner membrane phage-shock protein (NCBI) compare
b1171 b1171 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b4592 yccB +0.5 1.4 hypothetical protein (RefSeq) compare
b1336 ydaH +0.5 1.8 putative pump protein (transport) (VIMSS) compare
b1247 oppF +0.4 2.6 oligopeptide transporter subunit (NCBI) compare
b1243 oppA +0.4 2.9 oligopeptide transporter subunit (NCBI) compare
b2735 ygbI +0.4 2.3 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1217 chaB +0.4 1.1 cation transport regulator (NCBI) compare
b0931 pncB +0.4 2.8 nicotinate phosphoribosyltransferase (NCBI) compare
b4133 cadC +0.4 4.0 DNA-binding transcriptional activator (NCBI) compare
b1412 azoR +0.4 1.9 acyl carrier protein phosphodiesterase (NCBI) compare
b3894 fdoG +0.4 4.2 formate dehydrogenase-O, large subunit (NCBI) compare
b4294 insA-7 +0.4 1.3 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2953 yggU +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b0014 dnaK +0.4 1.3 molecular chaperone DnaK (NCBI) compare
b0617 citD +0.4 1.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b0210 yafE +0.4 1.7 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2156 lysP +0.4 3.5 lysine transporter (NCBI) compare
b3190 yrbA +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b4578 insP +0.4 2.1 no description compare
b4126 yjdI +0.4 3.9 hypothetical protein (NCBI) compare
b2634 yfjR +0.4 2.2 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0065 yabI +0.4 2.8 conserved inner membrane protein (NCBI) compare
b2063 yegH +0.4 3.2 putative transport protein (VIMSS) compare
b1147 ymfL +0.4 1.6 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0059 hepA +0.4 3.7 ATP-dependent helicase HepA (NCBI) compare
b2050 wcaI +0.4 1.4 predicted glycosyl transferase (NCBI) compare
b1942 fliJ +0.4 0.9 flagellar biosynthesis chaperone (NCBI) compare
b2198 ccmD +0.4 1.8 cytochrome c biogenesis protein (NCBI) compare
b1467 narY +0.4 2.4 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b1562 hokD +0.4 1.5 Qin prophage; small toxic polypeptide (NCBI) compare
b4468 glcE +0.4 2.3 glycolate oxidase FAD binding subunit (NCBI) compare
b3893 fdoH +0.4 3.3 formate dehydrogenase-O, Fe-S subunit (NCBI) compare
b3071 yqjI +0.4 1.5 predicted transcriptional regulator (NCBI) compare
b4533 ynfO +0.4 0.9 Qin prophage; predicted protein (NCBI) compare
b0261 mmuM +0.4 2.8 homocysteine methyltransferase (NCBI) compare
b1244 oppB +0.4 3.0 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2128 yehW +0.4 1.5 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1648 b1648 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3939 metB +0.4 2.7 cystathionine gamma-synthase (NCBI) compare
b1245 oppC +0.4 2.6 oligopeptide transporter subunit (NCBI) compare
b3670 ilvN +0.4 2.6 acetolactate synthase small subunit (NCBI) compare
b1550 b1550 +0.4 0.5 orf, hypothetical protein (VIMSS) compare
b1792 yeaO +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b2080 b2080 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b2186 yejK +0.4 2.9 nucleoid-associated protein NdpA (NCBI) compare
b2466 ypfG +0.4 2.0 hypothetical protein (NCBI) compare
b3009 yghB +0.4 2.5 conserved inner membrane protein (NCBI) compare
b3192 yrbC +0.4 3.0 predicted ABC-type organic solvent transporter (NCBI) compare
b2646 ypjF +0.4 1.4 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1946 fliN +0.4 2.0 flagellar motor switch protein (NCBI) compare
b4454 rdlD +0.4 2.0 sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR-D repeat, with toxic peptide LdrD (RefSeq) compare
b3126 garL +0.4 2.0 alpha-dehydro-beta-deoxy-D-glucarate aldolase (NCBI) compare
b3191 yrbB +0.4 2.7 orf, hypothetical protein (VIMSS) compare
b1074 flgC +0.4 1.3 flagellar basal-body rod protein C (NCBI) compare
b1729 ydjN +0.4 2.2 predicted transporter (NCBI) compare
b1560 ydfU +0.4 1.9 Qin prophage; predicted protein (RefSeq) compare
b2903 gcvP +0.4 3.0 glycine dehydrogenase (NCBI) compare
b1572 ydfB +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1728 ydjM +0.4 1.5 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1791 yeaN +0.4 1.8 predicted transporter (NCBI) compare
b0784 moaD +0.4 1.6 molybdopterin synthase, small subunit (NCBI) compare
b4394 yjjX +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1295 ymjA +0.4 1.7 hypothetical protein (NCBI) compare
b1219 ychN +0.4 1.9 hypothetical protein (NCBI) compare
b0292 yagY +0.4 2.4 hypothetical protein (NCBI) compare
b1427 rimL +0.4 1.7 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b4505 insX +0.4 0.7 predicted IS protein (RefSeq) compare
b1387 maoC +0.4 1.9 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1951 rcsA +0.4 0.2 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b3146 yraL +0.3 2.4 predicted methyltransferase (NCBI) compare
b4692 oweE +0.3 1.0 no description compare
b1348 lar +0.3 0.7 Rac prophage; restriction alleviation protein (NCBI) compare
b2886 ygfS +0.3 2.6 putative oxidoreductase, Fe-S subunit (VIMSS) compare
b3635 mutM +0.3 2.9 formamidopyrimidine-DNA glycosylase (NCBI) compare
b1565 ydfV +0.3 1.3 Qin prophage; predicted protein (NCBI) compare
b0431 cyoB +0.3 2.9 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b4551 yheV +0.3 1.0 hypothetical protein (NCBI) compare
b2440 eutC +0.3 2.4 ethanolamine ammonia-lyase small subunit (NCBI) compare
b1301 puuB +0.3 2.1 gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI) compare
b0975 hyaD +0.3 1.9 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b4194 ulaB +0.3 1.5 L-ascorbate-specific enzyme IIB component of PTS (NCBI) compare
b3891 fdhE +0.3 2.8 formate dehydrogenase accessory protein FdhE (NCBI) compare
b3131 agaR +0.3 1.4 DNA-binding transcriptional dual regulator (NCBI) compare
b4406 yaeP +0.3 1.3 hypothetical protein (NCBI) compare
b4269 yjgB +0.3 2.0 putative oxidoreductase (VIMSS) compare
b3031 yqiA +0.3 1.7 predicted esterase (NCBI) compare
b3658 selC +0.3 1.1 tRNA-Sec (NCBI) compare
b2267 elaA +0.3 1.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare


Specific Phenotypes

For 9 genes in this experiment

For stress 5-Fluorodihydropyrimidine-2,4-dione in Escherichia coli BW25113

For stress 5-Fluorodihydropyrimidine-2,4-dione across organisms