Experiment set56IT035 for Escherichia coli BW25113

M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

Group: stress
Media: M9 + 5-Fluorodihydropyrimidine-2,4-dione (5000 uM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, microplate, Anaerobic, at 37 (C)
By: Ben Guthrie on 2/8/22
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 10 genes in this experiment

For stress 5-Fluorodihydropyrimidine-2,4-dione in Escherichia coli BW25113

For stress 5-Fluorodihydropyrimidine-2,4-dione across organisms

SEED Subsystems

Subsystem	#Specific
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Arginine Biosynthesis extended	1
Lysine Biosynthesis DAP Pathway	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine biosynthesis
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-ornithine biosynthesis I	5	5	1
L-arginine degradation II (AST pathway)	5	5	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
aspartate superpathway	25	24	1