Experiment set54IT089 for Escherichia coli BW25113

Compare to:

mariner vector transformation; liquid outgrowth assay

200 most important genes:

  gene name fitness t score description  
b1422 ydcI -5.8 -8.4 putative transcriptional regulator LYSR-type (VIMSS) compare
b3912 cpxR -5.5 -9.9 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b0441 ppiD -5.2 -15.4 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b3094 exuR -4.9 -3.4 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b4174 hflK -4.9 -5.8 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2952 yggT -4.9 -7.8 predicted inner membrane protein (NCBI) compare
b4175 hflC -4.8 -7.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2101 yegW -4.8 -6.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2175 spr -4.7 -3.2 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0418 pgpA -4.5 -5.8 phosphatidylglycerophosphatase A (NCBI) compare
b2741 rpoS -4.4 -8.6 RNA polymerase sigma factor (NCBI) compare
b0970 yccA -4.4 -6.0 inner membrane protein (NCBI) compare
b3466 yhhL -4.3 -6.4 conserved inner membrane protein (NCBI) compare
b3932 hslV -4.3 -11.6 ATP-dependent protease peptidase subunit (NCBI) compare
b3611 yibN -4.2 -5.7 predicted rhodanese-related sulfurtransferase (NCBI) compare
b3931 hslU -4.0 -16.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3423 glpR -3.9 -10.3 DNA-binding transcriptional repressor (NCBI) compare
b1620 malI -3.9 -2.7 DNA-binding transcriptional repressor (NCBI) compare
b3037 ygiB -3.8 -5.9 conserved outer membrane protein (RefSeq) compare
b2450 yffS -3.8 -2.4 CPZ-55 prophage; predicted protein (RefSeq) compare
b3038 ygiC -3.7 -9.5 predicted enzyme (NCBI) compare
b4232 fbp -3.6 -3.5 fructose-1,6-bisphosphatase (NCBI) compare
b4241 treR -3.5 -11.8 trehalose repressor (NCBI) compare
b0407 yajC -3.4 -1.9 preprotein translocase subunit YajC (NCBI) compare
b3465 yhhF -3.4 -5.9 predicted methyltransferase (NCBI) compare
b0920 ycbC -3.2 -5.5 conserved inner membrane protein (NCBI) compare
b0908 aroA -3.1 -4.8 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4163 glyV -3.1 -1.2 tRNA-Gly (NCBI) compare
b1179 ycgL -3.1 -3.1 conserved protein (NCBI) compare
b3226 yhcK -3.0 -3.9 putative FADA-type transcriptional regulator (VIMSS) compare
b1961 dcm -3.0 -1.6 DNA cytosine methylase (NCBI) compare
b0462 acrB -3.0 -12.4 multidrug efflux system protein (NCBI) compare
b2501 ppk -3.0 -4.5 polyphosphate kinase (NCBI) compare
b2951 yggS -3.0 -5.2 predicted enzyme (NCBI) compare
b0142 folK -2.9 -0.8 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1255 yciC -2.9 -4.3 hypothetical protein (NCBI) compare
b3752 rbsK -2.9 -10.4 ribokinase (NCBI) compare
b1575 dicB -2.9 -2.1 Qin prophage; cell division inhibition protein (NCBI) compare
b3403 pck -2.9 -12.3 phosphoenolpyruvate carboxykinase (NCBI) compare
b4603 rseX -2.9 -0.8 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3443 yrhA -2.9 -1.5 orf, hypothetical protein (VIMSS) compare
b2037 rfbX -2.9 -9.9 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b0463 acrA -2.9 -9.2 multidrug efflux system (NCBI) compare
b2314 dedD -2.8 -3.9 putative lipoprotein (VIMSS) compare
b3205 yhbJ -2.8 -1.9 hypothetical protein (NCBI) compare
b1557 cspB -2.7 -2.1 Qin prophage; cold shock protein (NCBI) compare
b3790 rffC -2.7 -1.8 TDP-fucosamine acetyltransferase (RefSeq) compare
b0676 nagC -2.6 -6.1 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b2329 aroC -2.6 -2.5 chorismate synthase (NCBI) compare
b3891 fdhE -2.5 -8.1 formate dehydrogenase accessory protein FdhE (NCBI) compare
b1842 holE -2.5 -1.9 DNA polymerase III, theta subunit (NCBI) compare
b4699 fnrS -2.5 -0.9 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3884 yihW -2.5 -6.5 putative DEOR-type transcriptional regulator (VIMSS) compare
b3601 mtlR -2.4 -6.1 DNA-binding repressor (NCBI) compare
b1618 uidR -2.4 -3.2 DNA-binding transcriptional repressor (NCBI) compare
b3911 cpxA -2.4 -6.5 two-component sensor protein (NCBI) compare
b1323 tyrR -2.4 -4.2 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b1320 ycjW -2.4 -7.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b0145 dksA -2.4 -2.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b2618 b2618 -2.4 -3.0 orf, hypothetical protein (VIMSS) compare
b0113 pdhR -2.3 -4.6 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b4041 plsB -2.3 -1.5 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2362 yfdS -2.2 -4.5 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4529 ydbJ -2.2 -0.8 hypothetical protein (NCBI) compare
b3849 trkH -2.2 -5.0 potassium transporter (NCBI) compare
b4647 mokA -2.2 -1.1 no description compare
b1357 ydaS -2.1 -1.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b3753 rbsR -2.1 -10.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2642 yfjW -2.1 -1.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1025 ycdT -2.1 -1.4 predicted diguanylate cyclase (NCBI) compare
b3424 glpG -2.1 -6.9 predicted intramembrane serine protease (NCBI) compare
b1847 yebF -2.1 -1.0 predicted protein (RefSeq) compare
b1175 minD -2.1 -2.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1090 plsX -2.0 -3.1 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1482 osmC -2.0 -1.8 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b4025 pgi -2.0 -5.3 glucose-6-phosphate isomerase (NCBI) compare
b1304 pspA -1.9 -2.2 regulatory protein for phage-shock-protein operon (NCBI) compare
b0661 miaB -1.9 -8.8 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3404 envZ -1.9 -7.6 osmolarity sensor protein (NCBI) compare
b0436 tig -1.9 -5.4 trigger factor (NCBI) compare
b0460 hha -1.9 -4.7 modulator of gene expression, with H-NS (NCBI) compare
b2844 yqeF -1.9 -6.5 putative acyltransferase (VIMSS) compare
b0912 ihfB -1.9 -1.6 integration host factor subunit beta (NCBI) compare
b3289 rsmB -1.9 -9.3 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b3292 zntR -1.8 -3.9 zinc-responsive transcriptional regulator (NCBI) compare
b2014 yeeF -1.8 -4.8 putative amino acid/amine transport protein (VIMSS) compare
b1634 tppB -1.8 -3.6 putative tripeptide transporter permease (NCBI) compare
b3032 cpdA -1.8 -4.8 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b3497 yhiQ -1.8 -6.2 predicted SAM-dependent methyltransferase (NCBI) compare
b3438 gntR -1.7 -5.4 DNA-binding transcriptional repressor (NCBI) compare
b1630 rsxD -1.7 -4.3 electron transport complex protein RnfD (NCBI) compare
b1176 minC -1.7 -1.1 septum formation inhibitor (NCBI) compare
b2039 rfbA -1.7 -9.7 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2796 sdaC -1.7 -8.3 predicted serine transporter (NCBI) compare
b1878 flhE -1.7 -5.3 hypothetical protein (NCBI) compare
b4140 b4140 -1.7 -4.2 orf, hypothetical protein (VIMSS) compare
b1628 rsxB -1.7 -2.0 electron transport complex protein RnfB (NCBI) compare
b2910 zapA -1.7 -2.3 protein that localizes to the cytokinetic ring (NCBI) compare
b4451 ryhB -1.7 -1.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1524 yneH -1.7 -0.9 predicted glutaminase (NCBI) compare
b1627 rsxA -1.7 -2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1095 fabF -1.7 -3.4 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b2340 sixA -1.7 -2.2 phosphohistidine phosphatase (NCBI) compare
b0240 crl -1.6 -5.6 DNA-binding transcriptional regulator (NCBI) compare
b0934 ycbM -1.6 -1.9 putative transport system permease protein (VIMSS) compare
b2714 ascG -1.6 -5.2 DNA-binding transcriptional repressor (NCBI) compare
b2435 amiA -1.6 -4.0 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b0791 ybhQ -1.6 -2.3 predicted inner membrane protein (NCBI) compare
b4035 malK -1.6 -3.2 fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI) compare
b4450 arcZ -1.5 -3.0 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3673 emrD -1.5 -6.7 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b0406 tgt -1.5 -3.2 queuine tRNA-ribosyltransferase (NCBI) compare
b1685 ydiH -1.5 -1.2 predicted protein (RefSeq) compare
b3229 sspA -1.5 -1.0 stringent starvation protein A (NCBI) compare
b2513 yfgM -1.5 -2.3 hypothetical protein (NCBI) compare
b4519 icdC -1.5 -2.6 no description compare
b2934 cmtB -1.5 -0.8 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1523 yneG -1.5 -1.6 hypothetical protein (NCBI) compare
b1865 nudB -1.5 -0.8 dATP pyrophosphohydrolase (NCBI) compare
b1503 ydeR -1.5 -2.8 predicted fimbrial-like adhesin protein (NCBI) compare
b1550 b1550 -1.5 -1.1 orf, hypothetical protein (VIMSS) compare
b1111 ycfQ -1.5 -3.2 orf, hypothetical protein (VIMSS) compare
b0471 ybaB -1.4 -1.9 hypothetical protein (NCBI) compare
b3075 ebgR -1.4 -3.4 DNA-binding transcriptional repressor (NCBI) compare
b1632 rsxE -1.4 -5.3 NADH-ubiquinone oxidoreductase (NCBI) compare
b1642 slyA -1.4 -1.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4535 yniD -1.4 -0.8 predicted protein (RefSeq) compare
b0926 ycbK -1.4 -2.2 hypothetical protein (NCBI) compare
b2912 ygfA -1.4 -1.0 putative ligase (VIMSS) compare
b2260 menE -1.4 -5.7 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b1187 fadR -1.4 -2.8 fatty acid metabolism regulator (NCBI) compare
b1361 ydaW -1.4 -0.4 orf, hypothetical protein (VIMSS) compare
b2317 dedA -1.4 -2.9 conserved inner membrane protein (NCBI) compare
b4217 ytfK -1.4 -3.7 orf, hypothetical protein (VIMSS) compare
b0729 sucD -1.4 -1.3 succinyl-CoA synthetase subunit alpha (NCBI) compare
b4585 sroB -1.4 -1.2 Novel sRNA, function unknown (RefSeq) compare
b3727 pstC -1.4 -2.6 phosphate transporter subunit (NCBI) compare
b0728 sucC -1.4 -2.5 succinyl-CoA synthetase subunit beta (NCBI) compare
b2214 yojL -1.4 -3.1 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b2783 chpR -1.3 -1.7 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b1693 aroD -1.3 -2.3 3-dehydroquinate dehydratase (NCBI) compare
b1739 osmE -1.3 -1.9 DNA-binding transcriptional activator (NCBI) compare
b0767 ybhE -1.3 -1.6 6-phosphogluconolactonase (NCBI) compare
b1048 mdoG -1.3 -3.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b3484 yhhI -1.3 -2.6 predicted transposase (NCBI) compare
b3791 rffA -1.3 -0.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1556 b1556 -1.3 -1.7 orf, hypothetical protein (VIMSS) compare
b0542 renD -1.3 -2.1 orf, hypothetical protein (VIMSS) compare
b0382 yaiB -1.3 -5.1 hypothetical protein (NCBI) compare
b2964 nupG -1.3 -4.8 transport of nucleosides, permease protein (VIMSS) compare
b3381 yhfX -1.3 -4.9 predicted amino acid racemase (NCBI) compare
b1518 lsrG -1.3 -0.8 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b3529 yhjK -1.3 -7.9 predicted diguanylate cyclase (RefSeq) compare
b4594 ymgJ -1.3 -1.9 hypothetical protein (RefSeq) compare
b1827 kdgR -1.3 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3933 ftsN -1.2 -5.2 essential cell division protein (NCBI) compare
b3383 yhfZ -1.2 -3.8 hypothetical protein (NCBI) compare
b2264 menD -1.2 -4.5 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b3017 sufI -1.2 -5.7 repressor protein for FtsI (NCBI) compare
b3930 menA -1.2 -6.8 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b4184 yjfL -1.2 -3.6 conserved inner membrane protein (NCBI) compare
b0921 smtA -1.2 -4.0 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b4390 nadR -1.2 -5.6 probable nadAB transcriptional regulator (VIMSS) compare
b2643 yfjX -1.2 -2.4 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b1954 dsrA -1.2 -1.8 regulatory antisense RNA (NCBI) compare
b0617 citD -1.2 -2.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2376 ypdI -1.2 -3.1 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b2261 menC -1.2 -2.8 O-succinylbenzoate synthase (NCBI) compare
b2085 b2085 -1.2 -3.3 orf, hypothetical protein (VIMSS) compare
b2295 yfbV -1.2 -2.8 hypothetical protein (NCBI) compare
b4701 sokX -1.2 -0.6 sok-related sRNA, function unknown (RefSeq) compare
b4513 kdpF -1.2 -1.1 potassium ion accessory transporter subunit (NCBI) compare
b0197 metQ -1.2 -4.3 DL-methionine transporter subunit (NCBI) compare
b2510 b2510 -1.2 -2.3 orf, hypothetical protein (VIMSS) compare
b2540 hcaC -1.2 -2.6 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b2188 yejM -1.2 -4.3 predicted hydrolase, inner membrane (NCBI) compare
b3146 yraL -1.1 -4.5 predicted methyltransferase (NCBI) compare
b3890 yiiF -1.1 -2.6 orf, hypothetical protein (VIMSS) compare
b1953 b1953 -1.1 -2.1 orf, hypothetical protein (VIMSS) compare
b1753 ynjA -1.1 -1.6 hypothetical protein (NCBI) compare
b4416 rybA -1.1 -0.8 unknown RNA (NCBI) compare
b1007 b1007 -1.1 -1.0 orf, hypothetical protein (VIMSS) compare
b1558 cspF -1.1 -1.5 Qin prophage; cold shock protein (NCBI) compare
b2262 menB -1.1 -1.7 naphthoate synthase (NCBI) compare
b1689 b1689 -1.1 -2.2 orf, hypothetical protein (VIMSS) compare
b3259 prmA -1.1 -2.1 ribosomal protein L11 methyltransferase (NCBI) compare
b0730 mngR -1.1 -1.3 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4348 hsdS -1.1 -6.3 specificity determinant for hsdM and hsdR (NCBI) compare
b1832 yebR -1.1 -2.9 free methionine-(R)-sulfoxide reductase (RefSeq) compare
b1490 yddV -1.1 -3.8 predicted diguanylate cyclase (RefSeq) compare
b3131 agaR -1.1 -1.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4545 ypdJ -1.1 -1.5 no description compare
b3816 corA -1.1 -4.4 magnesium/nickel/cobalt transporter (NCBI) compare
b3663 yicN -1.1 -3.3 orf, hypothetical protein (VIMSS) compare
b1249 cls -1.1 -3.8 cardiolipin synthetase (NCBI) compare
b4557 yidD -1.1 -1.7 hypothetical protein (NCBI) compare
b4589 ylcI -1.1 -1.4 hypothetical protein (RefSeq) compare
b2896 ygfX -1.1 -4.1 hypothetical protein (NCBI) compare
b1629 rsxC -1.1 -3.3 electron transport complex protein RnfC (NCBI) compare
b1926 fliT -1.0 -1.4 predicted chaperone (NCBI) compare


Specific Phenotypes

For 68 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms