Experiment set54IT087 for Escherichia coli BW25113

Compare to:

mariner vector transformation; liquid outgrowth assay

200 most important genes:

  gene name fitness t score description  
b0441 ppiD -5.6 -10.8 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b3466 yhhL -5.6 -3.9 conserved inner membrane protein (NCBI) compare
b2101 yegW -5.1 -4.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3912 cpxR -5.1 -9.2 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b3038 ygiC -5.1 -10.7 predicted enzyme (NCBI) compare
b4175 hflC -4.6 -5.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1104 ycfL -4.6 -1.7 hypothetical protein (NCBI) compare
b4174 hflK -4.5 -4.4 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3611 yibN -4.5 -3.1 predicted rhodanese-related sulfurtransferase (NCBI) compare
b0970 yccA -4.4 -4.1 inner membrane protein (NCBI) compare
b2952 yggT -4.4 -6.2 predicted inner membrane protein (NCBI) compare
b3094 exuR -4.4 -3.0 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b3465 yhhF -4.2 -4.1 predicted methyltransferase (NCBI) compare
b3932 hslV -4.2 -11.5 ATP-dependent protease peptidase subunit (NCBI) compare
b1255 yciC -4.2 -4.5 hypothetical protein (NCBI) compare
b3931 hslU -4.1 -13.8 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0462 acrB -4.1 -15.6 multidrug efflux system protein (NCBI) compare
b3226 yhcK -4.1 -2.8 putative FADA-type transcriptional regulator (VIMSS) compare
b0418 pgpA -4.0 -4.8 phosphatidylglycerophosphatase A (NCBI) compare
b4241 treR -3.8 -11.4 trehalose repressor (NCBI) compare
b0463 acrA -3.7 -9.7 multidrug efflux system (NCBI) compare
b1422 ydcI -3.7 -5.0 putative transcriptional regulator LYSR-type (VIMSS) compare
b3037 ygiB -3.6 -4.4 conserved outer membrane protein (RefSeq) compare
b1175 minD -3.6 -2.3 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2844 yqeF -3.5 -8.7 putative acyltransferase (VIMSS) compare
b2141 yohJ -3.4 -2.4 hypothetical protein (NCBI) compare
b2741 rpoS -3.4 -5.8 RNA polymerase sigma factor (NCBI) compare
b0113 pdhR -3.4 -4.0 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b2314 dedD -3.3 -3.7 putative lipoprotein (VIMSS) compare
b4232 fbp -3.2 -2.2 fructose-1,6-bisphosphatase (NCBI) compare
b3423 glpR -3.2 -7.6 DNA-binding transcriptional repressor (NCBI) compare
b2796 sdaC -3.1 -9.1 predicted serine transporter (NCBI) compare
b1882 cheY -2.9 -0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2501 ppk -2.9 -2.0 polyphosphate kinase (NCBI) compare
b0990 cspG -2.8 -1.0 DNA-binding transcriptional regulator (NCBI) compare
b1620 malI -2.8 -1.9 DNA-binding transcriptional repressor (NCBI) compare
b0142 folK -2.8 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b0908 aroA -2.7 -5.2 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3891 fdhE -2.7 -6.5 formate dehydrogenase accessory protein FdhE (NCBI) compare
b2939 yqgB -2.7 -1.1 predicted protein (RefSeq) compare
b4557 yidD -2.7 -3.2 hypothetical protein (NCBI) compare
b1480 sra -2.6 -1.3 30S ribosomal subunit protein S22 (NCBI) compare
b4603 rseX -2.6 -0.6 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0436 tig -2.6 -6.4 trigger factor (NCBI) compare
b3884 yihW -2.5 -5.0 putative DEOR-type transcriptional regulator (VIMSS) compare
b1482 osmC -2.5 -1.8 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3198 kdsC -2.5 -1.9 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2707 srlR -2.5 -3.8 DNA-bindng transcriptional repressor (NCBI) compare
b0791 ybhQ -2.5 -3.4 predicted inner membrane protein (NCBI) compare
b1304 pspA -2.5 -2.3 regulatory protein for phage-shock-protein operon (NCBI) compare
b0676 nagC -2.5 -4.3 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b4025 pgi -2.5 -7.0 glucose-6-phosphate isomerase (NCBI) compare
b3497 yhiQ -2.5 -7.1 predicted SAM-dependent methyltransferase (NCBI) compare
b0706 ybfD -2.4 -1.7 hypothetical protein (NCBI) compare
b1493 gadB -2.4 -0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b3443 yrhA -2.4 -1.0 orf, hypothetical protein (VIMSS) compare
b2329 aroC -2.3 -1.6 chorismate synthase (NCBI) compare
b3601 mtlR -2.3 -5.6 DNA-binding repressor (NCBI) compare
b2037 rfbX -2.3 -6.3 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b0730 mngR -2.3 -1.4 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4655 ythA -2.2 -1.4 expressed protein (RefSeq) compare
b3849 trkH -2.2 -1.5 potassium transporter (NCBI) compare
b0548 ninE -2.2 -3.7 DLP12 prophage; conserved protein (NCBI) compare
b4699 fnrS -2.2 -0.7 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0407 yajC -2.2 -1.7 preprotein translocase subunit YajC (NCBI) compare
b3146 yraL -2.2 -5.4 predicted methyltransferase (NCBI) compare
b3790 rffC -2.2 -1.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b1231 tyrT -2.1 -0.6 tRNA-Tyr (NCBI) compare
b2295 yfbV -2.1 -3.0 hypothetical protein (NCBI) compare
b1550 b1550 -2.1 -0.8 orf, hypothetical protein (VIMSS) compare
b3259 prmA -2.1 -3.4 ribosomal protein L11 methyltransferase (NCBI) compare
b3752 rbsK -2.1 -8.7 ribokinase (NCBI) compare
b1150 ymfR -2.1 -0.8 e14 prophage; predicted protein (NCBI) compare
b1961 dcm -2.1 -1.1 DNA cytosine methylase (NCBI) compare
b2947 gshB -2.1 -2.4 glutathione synthetase (NCBI) compare
b1134 ymfB -2.0 -1.6 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b2156 lysP -2.0 -7.6 lysine transporter (NCBI) compare
b2175 spr -2.0 -2.6 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b4585 sroB -2.0 -1.1 Novel sRNA, function unknown (RefSeq) compare
b0928 aspC -2.0 -3.2 aspartate aminotransferase, PLP-dependent (NCBI) compare
b3205 yhbJ -2.0 -1.9 hypothetical protein (NCBI) compare
b1323 tyrR -2.0 -2.4 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b2183 rsuA -2.0 -2.2 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b2313 cvpA -1.9 -3.1 membrane protein required for colicin V production (NCBI) compare
b2331 yfcN -1.9 -4.9 hypothetical protein (NCBI) compare
b3404 envZ -1.9 -9.0 osmolarity sensor protein (NCBI) compare
b0145 dksA -1.9 -1.9 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3403 pck -1.9 -7.8 phosphoenolpyruvate carboxykinase (NCBI) compare
b1557 cspB -1.9 -1.4 Qin prophage; cold shock protein (NCBI) compare
b4532 yncN -1.9 -1.9 orf, hypothetical protein (VIMSS) compare
b1147 ymfL -1.9 -2.1 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3289 rsmB -1.9 -8.7 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b2964 nupG -1.9 -7.9 transport of nucleosides, permease protein (VIMSS) compare
b1753 ynjA -1.9 -1.3 hypothetical protein (NCBI) compare
b1954 dsrA -1.8 -1.8 regulatory antisense RNA (NCBI) compare
b2340 sixA -1.8 -2.0 phosphohistidine phosphatase (NCBI) compare
b1563 relE -1.8 -1.2 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b3032 cpdA -1.8 -5.7 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b3424 glpG -1.8 -6.3 predicted intramembrane serine protease (NCBI) compare
b3617 kbl -1.8 -3.7 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b2263 yfbB -1.7 -1.5 predicted peptidase (NCBI) compare
b0097 yacA -1.7 -2.6 orf, hypothetical protein (VIMSS) compare
b3383 yhfZ -1.7 -4.0 hypothetical protein (NCBI) compare
b2513 yfgM -1.7 -2.8 hypothetical protein (NCBI) compare
b3977 tyrU -1.7 -0.5 tRNA-Tyr (NCBI) compare
b2362 yfdS -1.7 -2.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2014 yeeF -1.7 -4.1 putative amino acid/amine transport protein (VIMSS) compare
b0932 pepN -1.7 -6.1 aminopeptidase N (NCBI) compare
b1038 csgF -1.7 -2.3 predicted transport protein (NCBI) compare
b2261 menC -1.7 -3.9 O-succinylbenzoate synthase (NCBI) compare
b0240 crl -1.7 -5.4 DNA-binding transcriptional regulator (NCBI) compare
b1179 ycgL -1.6 -1.6 conserved protein (NCBI) compare
b2260 menE -1.6 -6.9 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b4647 mokA -1.6 -0.7 no description compare
b0782 moaB -1.6 -1.4 molybdopterin biosynthesis protein B (NCBI) compare
b4349 hsdM -1.6 -5.7 DNA methylase M (NCBI) compare
b3753 rbsR -1.6 -7.6 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0218 yafU -1.6 -2.4 predicted inner membrane protein (RefSeq) compare
b2264 menD -1.6 -4.4 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1090 plsX -1.6 -2.2 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1642 slyA -1.6 -1.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0920 ycbC -1.6 -2.7 conserved inner membrane protein (NCBI) compare
b1842 holE -1.5 -1.1 DNA polymerase III, theta subunit (NCBI) compare
b0846 ybjK -1.5 -4.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4390 nadR -1.5 -6.2 probable nadAB transcriptional regulator (VIMSS) compare
b4217 ytfK -1.5 -3.6 orf, hypothetical protein (VIMSS) compare
b4692 oweE -1.5 -1.8 no description compare
b0798 ybiA -1.5 -1.4 hypothetical protein (NCBI) compare
b2114 metG -1.5 -1.3 methionyl-tRNA synthetase (NCBI) compare
b4393 trpR -1.5 -3.6 Trp operon repressor (NCBI) compare
b3726 pstA -1.5 -1.0 phosphate transporter subunit (NCBI) compare
b2258 b2258 -1.5 -0.7 putative transport/receptor protein (VIMSS) compare
b3238 yhcN -1.5 -0.5 orf, hypothetical protein (VIMSS) compare
b0937 ssuE -1.4 -1.7 NAD(P)H-dependent FMN reductase (NCBI) compare
b1408 b1408 -1.4 -2.8 probable enzyme (VIMSS) compare
b0976 hyaE -1.4 -2.0 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b0661 miaB -1.4 -5.1 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b2859 ygeO -1.4 -1.5 hypothetical protein (RefSeq) compare
b0728 sucC -1.4 -2.1 succinyl-CoA synthetase subunit beta (NCBI) compare
b1507 hipA -1.4 -3.1 regulator with hipB (NCBI) compare
b2783 chpR -1.4 -1.3 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b2393 nupC -1.4 -3.5 nucleoside (except guanosine) transporter (NCBI) compare
b1070 flgN -1.4 -0.7 export chaperone for FlgK and FlgL (NCBI) compare
b1125 potB -1.4 -1.7 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1272 sohB -1.4 -3.5 predicted inner membrane peptidase (NCBI) compare
b0707 ybgA -1.4 -2.7 hypothetical protein (NCBI) compare
b2262 menB -1.4 -1.5 naphthoate synthase (NCBI) compare
b4519 icdC -1.3 -2.1 no description compare
b2209 eco -1.3 -1.1 ecotin precursor (NCBI) compare
b1249 cls -1.3 -3.5 cardiolipin synthetase (NCBI) compare
b1095 fabF -1.3 -2.2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1324 tpx -1.3 -1.9 thiol peroxidase (NCBI) compare
b1871 yecP -1.3 -2.0 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b1618 uidR -1.3 -2.3 DNA-binding transcriptional repressor (NCBI) compare
b2510 b2510 -1.3 -1.7 orf, hypothetical protein (VIMSS) compare
b1670 ydhU -1.3 -3.2 predicted cytochrome (NCBI) compare
b1233 ychJ -1.3 -2.2 hypothetical protein (NCBI) compare
b4631 ykgQ -1.3 -0.7 no description compare
b1907 tyrP -1.3 -2.6 tyrosine transporter (NCBI) compare
b4431 rprA -1.3 -1.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2618 b2618 -1.3 -2.0 orf, hypothetical protein (VIMSS) compare
b2004 yeeU -1.3 -2.2 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b3098 yqjD -1.3 -2.0 hypothetical protein (NCBI) compare
b2272 yfbM -1.3 -2.1 hypothetical protein (NCBI) compare
b0502 ylbG -1.3 -2.0 no description compare
b1157 stfE -1.3 -4.3 no description compare
b1630 rsxD -1.3 -2.5 electron transport complex protein RnfD (NCBI) compare
b3933 ftsN -1.3 -4.6 essential cell division protein (NCBI) compare
b1728 ydjM -1.3 -1.6 predicted inner membrane protein regulated by LexA (RefSeq) compare
b0881 clpS -1.3 -0.8 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b1594 dgsA -1.3 -1.4 DNA-binding transcriptional repressor (NCBI) compare
b1245 oppC -1.3 -2.8 oligopeptide transporter subunit (NCBI) compare
b2910 zapA -1.3 -2.7 protein that localizes to the cytokinetic ring (NCBI) compare
b2221 atoD -1.2 -2.0 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b1176 minC -1.2 -0.6 septum formation inhibitor (NCBI) compare
b0729 sucD -1.2 -0.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1187 fadR -1.2 -2.0 fatty acid metabolism regulator (NCBI) compare
b2039 rfbA -1.2 -6.3 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2971 yghG -1.2 -4.2 hypothetical protein (NCBI) compare
b3930 menA -1.2 -6.1 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b4235 pmbA -1.2 -4.7 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b3263 yhdU -1.2 -3.4 predicted membrane protein (NCBI) compare
b1338 ydaJ -1.2 -2.6 putative aminohydrolase (EC 3.5.1.14) (VIMSS) compare
b0585 fes -1.2 -1.6 enterobactin/ferric enterobactin esterase (NCBI) compare
b2424 cysU -1.2 -1.4 sulfate/thiosulfate transporter subunit (NCBI) compare
b3438 gntR -1.2 -3.1 DNA-binding transcriptional repressor (NCBI) compare
b3017 sufI -1.2 -5.5 repressor protein for FtsI (NCBI) compare
b0605 ahpC -1.2 -1.8 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1114 mfd -1.2 -3.2 transcription-repair coupling factor (NCBI) compare
b0998 torD -1.2 -0.6 chaperone protein TorD (NCBI) compare
b0946 ycbW -1.2 -2.4 orf, hypothetical protein (VIMSS) compare
b3673 emrD -1.1 -4.6 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b0564 appY -1.1 -0.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0767 ybhE -1.1 -1.3 6-phosphogluconolactonase (NCBI) compare
b1250 kch -1.1 -4.0 voltage-gated potassium channel (NCBI) compare
b2435 amiA -1.1 -2.1 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b0632 dacA -1.1 -3.5 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0866 ybjQ -1.1 -0.6 hypothetical protein (NCBI) compare
b2593 yfiH -1.1 -4.7 hypothetical protein (NCBI) compare
b1361 ydaW -1.1 -0.2 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 59 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms