Experiment set54IT086 for Escherichia coli BW25113

Compare to:

vcDART vector transformation; liquid outgrowth assay

200 most important genes:

  gene name fitness t score description  
b3912 cpxR -5.0 -3.5 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b2952 yggT -4.9 -4.8 predicted inner membrane protein (NCBI) compare
b1422 ydcI -4.8 -7.3 putative transcriptional regulator LYSR-type (VIMSS) compare
b0970 yccA -4.7 -3.2 inner membrane protein (NCBI) compare
b3107 yhaL -4.6 -1.4 orf, hypothetical protein (VIMSS) compare
b4595 yciY -4.5 -2.0 hypothetical protein (RefSeq) compare
b0998 torD -4.4 -1.3 chaperone protein TorD (NCBI) compare
b4175 hflC -4.1 -2.8 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0441 ppiD -3.9 -11.1 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b1476 fdnI -3.8 -1.2 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b4538 yoeF -3.7 -1.6 hypothetical protein (RefSeq) compare
b2101 yegW -3.7 -5.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4174 hflK -3.7 -3.6 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b4699 fnrS -3.6 -1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1758 b1758 -3.6 -1.1 putative cytochrome oxidase (VIMSS) compare
b3198 kdsC -3.5 -2.4 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3094 exuR -3.5 -2.4 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b2953 yggU -3.3 -1.9 orf, hypothetical protein (VIMSS) compare
b3932 hslV -3.1 -9.7 ATP-dependent protease peptidase subunit (NCBI) compare
b0462 acrB -3.1 -10.7 multidrug efflux system protein (NCBI) compare
b4431 rprA -3.0 -1.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2904 gcvH -2.9 -1.8 glycine cleavage system protein H (NCBI) compare
b2951 yggS -2.9 -4.0 predicted enzyme (NCBI) compare
b3465 yhhF -2.9 -3.5 predicted methyltransferase (NCBI) compare
b2698 recX -2.9 -2.1 RecA regulator RecX (NCBI) compare
b3977 tyrU -2.9 -0.9 tRNA-Tyr (NCBI) compare
b0912 ihfB -2.9 -2.0 integration host factor subunit beta (NCBI) compare
b3403 pck -2.9 -9.4 phosphoenolpyruvate carboxykinase (NCBI) compare
b3037 ygiB -2.9 -4.3 conserved outer membrane protein (RefSeq) compare
b1175 minD -2.9 -2.0 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1954 dsrA -2.8 -1.7 regulatory antisense RNA (NCBI) compare
b1634 tppB -2.8 -3.9 putative tripeptide transporter permease (NCBI) compare
b2741 rpoS -2.8 -5.2 RNA polymerase sigma factor (NCBI) compare
b1573 ydfC -2.7 -1.2 Qin prophage; predicted protein (RefSeq) compare
b1953 b1953 -2.7 -2.2 orf, hypothetical protein (VIMSS) compare
b3884 yihW -2.7 -4.5 putative DEOR-type transcriptional regulator (VIMSS) compare
b2642 yfjW -2.7 -1.4 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2037 rfbX -2.7 -5.7 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b2969 yghE -2.6 -2.6 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b2579 yfiD -2.6 -2.0 pyruvate formate lyase subunit (NCBI) compare
b1523 yneG -2.6 -1.4 hypothetical protein (NCBI) compare
b3226 yhcK -2.6 -2.5 putative FADA-type transcriptional regulator (VIMSS) compare
b0627 tatE -2.5 -2.4 twin arginine translocase protein E (NCBI) compare
b1882 cheY -2.5 -0.8 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2175 spr -2.4 -2.8 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1563 relE -2.4 -1.6 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b1266 yciV -2.4 -2.6 hypothetical protein (NCBI) compare
b1171 b1171 -2.4 -1.6 orf, hypothetical protein (VIMSS) compare
b2814 metZ -2.4 -0.7 tRNA-Met (NCBI) compare
b0946 ycbW -2.4 -3.6 orf, hypothetical protein (VIMSS) compare
b0523 purE -2.4 -3.3 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4232 fbp -2.4 -2.3 fructose-1,6-bisphosphatase (NCBI) compare
b3466 yhhL -2.4 -3.4 conserved inner membrane protein (NCBI) compare
b1588 ynfF -2.3 -2.9 oxidoreductase subunit (RefSeq) compare
b2689 yqaA -2.3 -2.2 conserved inner membrane protein (NCBI) compare
b1164 ycgZ -2.3 -1.3 hypothetical protein (NCBI) compare
b3752 rbsK -2.3 -7.0 ribokinase (NCBI) compare
b1070 flgN -2.3 -1.0 export chaperone for FlgK and FlgL (NCBI) compare
b0463 acrA -2.3 -5.7 multidrug efflux system (NCBI) compare
b0142 folK -2.3 -0.7 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b0407 yajC -2.3 -1.4 preprotein translocase subunit YajC (NCBI) compare
b0920 ycbC -2.3 -3.3 conserved inner membrane protein (NCBI) compare
b0113 pdhR -2.2 -3.1 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b1865 nudB -2.2 -1.2 dATP pyrophosphohydrolase (NCBI) compare
b2501 ppk -2.2 -1.5 polyphosphate kinase (NCBI) compare
b3931 hslU -2.2 -7.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1090 plsX -2.2 -1.5 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4241 treR -2.2 -7.1 trehalose repressor (NCBI) compare
b4451 ryhB -2.2 -1.6 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1548 nohA -2.2 -1.3 Qin prophage; predicted packaging protein (RefSeq) compare
b2215 ompC -2.1 -3.8 outer membrane porin protein C (NCBI) compare
b0436 tig -2.1 -5.2 trigger factor (NCBI) compare
b2421 cysM -2.1 -2.5 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b2844 yqeF -2.1 -5.2 putative acyltransferase (VIMSS) compare
b3100 yqjK -2.0 -2.5 hypothetical protein (NCBI) compare
b3611 yibN -2.0 -3.6 predicted rhodanese-related sulfurtransferase (NCBI) compare
b0990 cspG -2.0 -1.1 DNA-binding transcriptional regulator (NCBI) compare
b0035 caiE -2.0 -3.6 possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS) compare
b1540 ydfH -2.0 -1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2586 yfiM -2.0 -2.4 predicted protein (RefSeq) compare
b1926 fliT -2.0 -1.6 predicted chaperone (NCBI) compare
b2494 yfgC -2.0 -4.3 predicted peptidase (NCBI) compare
b1400 paaY -2.0 -1.7 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b4450 arcZ -2.0 -2.1 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b2990 hybG -2.0 -1.8 hydrogenase 2 accessory protein (NCBI) compare
b3383 yhfZ -2.0 -4.9 hypothetical protein (NCBI) compare
b0263 afuB -2.0 -2.0 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b1837 yebW -2.0 -2.0 orf, hypothetical protein (VIMSS) compare
b1429 tehA -1.9 -1.0 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b4608 ryfC -1.9 -2.3 Novel sRNA, function unknown (RefSeq) compare
b3228 sspB -1.9 -2.9 ClpXP protease specificity-enhancing factor (NCBI) compare
b0661 miaB -1.9 -6.9 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b0143 pcnB -1.9 -1.8 poly(A) polymerase I (VIMSS) compare
b4551 yheV -1.9 -1.5 hypothetical protein (NCBI) compare
b4413 sokC -1.9 -1.0 regulatory antisense RNA (NCBI) compare
b2618 b2618 -1.8 -1.8 orf, hypothetical protein (VIMSS) compare
b2707 srlR -1.8 -3.4 DNA-bindng transcriptional repressor (NCBI) compare
b4203 rplI -1.8 -2.7 50S ribosomal protein L9 (NCBI) compare
b0908 aroA -1.8 -3.0 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4025 pgi -1.8 -5.8 glucose-6-phosphate isomerase (NCBI) compare
b2259 pmrD -1.8 -0.8 polymyxin resistance protein B (VIMSS) compare
b1752 ydjZ -1.8 -2.4 conserved inner membrane protein (NCBI) compare
b1524 yneH -1.8 -0.8 predicted glutaminase (NCBI) compare
b3423 glpR -1.8 -5.0 DNA-binding transcriptional repressor (NCBI) compare
b1487 ddpA -1.8 -3.4 D-ala-D-a la transporter subunit (NCBI) compare
b1569 dicC -1.8 -1.9 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b3529 yhjK -1.8 -8.3 predicted diguanylate cyclase (RefSeq) compare
b1514 lsrC -1.8 -1.2 AI2 transporter (NCBI) compare
b2329 aroC -1.8 -1.2 chorismate synthase (NCBI) compare
b3038 ygiC -1.8 -4.1 predicted enzyme (NCBI) compare
b0418 pgpA -1.8 -2.8 phosphatidylglycerophosphatase A (NCBI) compare
b0782 moaB -1.8 -1.4 molybdopterin biosynthesis protein B (NCBI) compare
b4557 yidD -1.7 -2.1 hypothetical protein (NCBI) compare
b1095 fabF -1.7 -3.7 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1632 rsxE -1.7 -3.3 NADH-ubiquinone oxidoreductase (NCBI) compare
b0730 mngR -1.7 -1.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b0881 clpS -1.7 -1.1 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b0542 renD -1.7 -1.8 orf, hypothetical protein (VIMSS) compare
b1480 sra -1.7 -1.2 30S ribosomal subunit protein S22 (NCBI) compare
b1085 b1085 -1.7 -1.4 orf, hypothetical protein (VIMSS) compare
b4503 yafF -1.7 -0.7 no description compare
b2209 eco -1.7 -1.3 ecotin precursor (NCBI) compare
b0249 ykfF -1.7 -1.3 CP4-6 prophage; predicted protein (NCBI) compare
b2807 ygdD -1.7 -2.8 conserved inner membrane protein (NCBI) compare
b2910 zapA -1.7 -1.8 protein that localizes to the cytokinetic ring (NCBI) compare
b3443 yrhA -1.7 -0.9 orf, hypothetical protein (VIMSS) compare
b2673 nrdH -1.7 -1.5 glutaredoxin-like protein (NCBI) compare
b2945 endA -1.6 -2.9 DNA-specific endonuclease I (NCBI) compare
b1696 ydiP -1.6 -1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b4695 ykgT -1.6 -1.3 no description compare
b1656 sodB -1.6 -2.5 superoxide dismutase, Fe (NCBI) compare
b2317 dedA -1.6 -3.4 conserved inner membrane protein (NCBI) compare
b3836 b3836 -1.6 -2.5 orf, hypothetical protein (VIMSS) compare
b2039 rfbA -1.6 -8.0 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2003 yeeT -1.6 -1.8 CP4-44 prophage; predicted protein (NCBI) compare
b0082 mraW -1.6 -1.9 S-adenosyl-methyltransferase (NCBI) compare
b4246 pyrL -1.6 -1.6 pyrBI operon leader peptide (NCBI) compare
b1334 fnr -1.6 -1.4 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0503 ybbB -1.6 -1.7 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b3781 trxA -1.6 -1.9 thioredoxin 1 (VIMSS) compare
b1394 paaG -1.6 -2.0 enoyl-CoA hydratase (NCBI) compare
b1742 b1742 -1.6 -1.7 orf, hypothetical protein (VIMSS) compare
b3289 rsmB -1.6 -5.2 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b4585 sroB -1.6 -0.9 Novel sRNA, function unknown (RefSeq) compare
b1105 ycfM -1.5 -1.6 predicted outer membrane lipoprotein (NCBI) compare
b2543 yphA -1.5 -3.6 predicted inner membrane protein (RefSeq) compare
b0015 dnaJ -1.5 -3.9 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1399 paaX -1.5 -2.5 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b4571 wbbL -1.5 -1.6 no description compare
b4501 torI -1.5 -2.3 response regulator inhibitor for tor operon (NCBI) compare
b2051 wcaH -1.5 -0.8 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2476 purC -1.5 -1.8 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1626 b1626 -1.5 -3.6 orf, hypothetical protein (VIMSS) compare
b3790 rffC -1.5 -1.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b0406 tgt -1.5 -3.1 queuine tRNA-ribosyltransferase (NCBI) compare
b0632 dacA -1.5 -5.0 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3649 rpoZ -1.5 -1.0 DNA-directed RNA polymerase subunit omega (NCBI) compare
b4217 ytfK -1.5 -3.2 orf, hypothetical protein (VIMSS) compare
b3086 ygjQ -1.4 -1.7 predicted thioredoxin-like (NCBI) compare
b4541 yehK -1.4 -2.2 hypothetical protein (NCBI) compare
b1593 b1593 -1.4 -2.2 orf, hypothetical protein (VIMSS) compare
b1878 flhE -1.4 -2.7 hypothetical protein (NCBI) compare
b1709 btuD -1.4 -0.6 vitamin B12-transporter ATPase (NCBI) compare
b0828 iaaA -1.4 -1.9 L-asparaginase (NCBI) compare
b2331 yfcN -1.4 -4.0 hypothetical protein (NCBI) compare
b1304 pspA -1.4 -2.1 regulatory protein for phage-shock-protein operon (NCBI) compare
b3017 sufI -1.4 -4.9 repressor protein for FtsI (NCBI) compare
b1322 ycjF -1.4 -2.7 hypothetical protein (NCBI) compare
b0866 ybjQ -1.4 -0.7 hypothetical protein (NCBI) compare
b4045 yjbJ -1.4 -1.5 predicted stress response protein (NCBI) compare
b2859 ygeO -1.4 -1.0 hypothetical protein (RefSeq) compare
b3753 rbsR -1.4 -4.8 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b4596 yciZ -1.4 -0.8 hypothetical protein (RefSeq) compare
b1503 ydeR -1.4 -1.7 predicted fimbrial-like adhesin protein (NCBI) compare
b2340 sixA -1.4 -1.4 phosphohistidine phosphatase (NCBI) compare
b4394 yjjX -1.4 -1.7 orf, hypothetical protein (VIMSS) compare
b0101 yacG -1.4 -1.7 zinc-binding protein (NCBI) compare
b2187 yejL -1.4 -1.5 hypothetical protein (NCBI) compare
b1482 osmC -1.4 -1.3 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3766 ilvL -1.4 -1.9 ilvG operon leader peptide (NCBI) compare
b2938 speA -1.4 -4.2 arginine decarboxylase (NCBI) compare
b2602 yfiL -1.4 -4.1 orf, hypothetical protein (VIMSS) compare
b2398 yfeC -1.4 -2.2 orf, hypothetical protein (VIMSS) compare
b4191 ulaR -1.4 -1.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0556 rzpD -1.4 -1.8 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b1793 yoaF -1.4 -1.3 conserved outer membrane protein (NCBI) compare
b1789 yeaL -1.4 -2.2 conserved inner membrane protein (NCBI) compare
b0676 nagC -1.4 -3.9 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b2248 yfaX -1.3 -1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b4035 malK -1.3 -3.5 fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI) compare
b0746 valZ -1.3 -0.9 tRNA-Val (NCBI) compare
b0858 ybjO -1.3 -1.1 predicted inner membrane protein (NCBI) compare
b3029 ygiN -1.3 -1.6 quinol monooxygenase (NCBI) compare
b3032 cpdA -1.3 -3.9 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b2512 yfgL -1.3 -2.5 protein assembly complex, lipoprotein component (NCBI) compare
b1804 rnd -1.3 -1.8 ribonuclease D (NCBI) compare
b0850 ybjC -1.3 -1.3 predicted inner membrane protein (NCBI) compare
b2012 yeeD -1.3 -1.7 hypothetical protein (NCBI) compare
b1814 sdaA -1.3 -2.5 L-serine deaminase I (NCBI) compare
b3347 fkpA -1.3 -5.5 FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI) compare


Specific Phenotypes

For 30 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms