Experiment set54IT085 for Escherichia coli BW25113

Compare to:

vcDART vector transformation; liquid outgrowth assay

200 most important genes:

  gene name fitness t score description  
b3912 cpxR -4.9 -7.5 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b1382 ynbE -4.6 -1.7 predicted lipoprotein (NCBI) compare
b4175 hflC -4.5 -3.1 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1823 cspC -4.3 -3.0 stress protein, member of the CspA-family (NCBI) compare
b3038 ygiC -4.2 -10.5 predicted enzyme (NCBI) compare
b4174 hflK -4.0 -4.7 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3094 exuR -4.0 -2.7 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b1476 fdnI -3.8 -1.4 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b3465 yhhF -3.7 -3.6 predicted methyltransferase (NCBI) compare
b3466 yhhL -3.6 -5.5 conserved inner membrane protein (NCBI) compare
b0441 ppiD -3.6 -12.5 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b1628 rsxB -3.6 -2.9 electron transport complex protein RnfB (NCBI) compare
b3226 yhcK -3.6 -2.5 putative FADA-type transcriptional regulator (VIMSS) compare
b0920 ycbC -3.4 -3.1 conserved inner membrane protein (NCBI) compare
b2101 yegW -3.3 -5.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2904 gcvH -3.3 -2.2 glycine cleavage system protein H (NCBI) compare
b2707 srlR -3.2 -6.9 DNA-bindng transcriptional repressor (NCBI) compare
b3977 tyrU -3.2 -1.2 tRNA-Tyr (NCBI) compare
b1961 dcm -3.2 -1.6 DNA cytosine methylase (NCBI) compare
b0912 ihfB -3.2 -2.2 integration host factor subunit beta (NCBI) compare
b1255 yciC -3.1 -4.6 hypothetical protein (NCBI) compare
b2175 spr -3.1 -3.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0836 yliH -3.1 -3.1 hypothetical protein (NCBI) compare
b0970 yccA -3.1 -3.3 inner membrane protein (NCBI) compare
b3037 ygiB -3.0 -6.0 conserved outer membrane protein (RefSeq) compare
b4241 treR -3.0 -11.0 trehalose repressor (NCBI) compare
b0019 nhaA -2.9 -6.3 pH-dependent sodium/proton antiporter (NCBI) compare
b2314 dedD -2.9 -3.8 putative lipoprotein (VIMSS) compare
b0418 pgpA -2.9 -4.4 phosphatidylglycerophosphatase A (NCBI) compare
b2952 yggT -2.9 -4.6 predicted inner membrane protein (NCBI) compare
b1422 ydcI -2.8 -6.0 putative transcriptional regulator LYSR-type (VIMSS) compare
b0676 nagC -2.8 -5.3 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b4232 fbp -2.8 -2.7 fructose-1,6-bisphosphatase (NCBI) compare
b4450 arcZ -2.8 -3.3 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1217 chaB -2.7 -1.7 cation transport regulator (NCBI) compare
b3611 yibN -2.6 -4.7 predicted rhodanese-related sulfurtransferase (NCBI) compare
b0407 yajC -2.6 -1.8 preprotein translocase subunit YajC (NCBI) compare
b3836 b3836 -2.6 -2.9 orf, hypothetical protein (VIMSS) compare
b2646 ypjF -2.5 -1.8 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1865 nudB -2.5 -1.6 dATP pyrophosphohydrolase (NCBI) compare
b1882 cheY -2.5 -0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3403 pck -2.4 -8.5 phosphoenolpyruvate carboxykinase (NCBI) compare
b2618 b2618 -2.4 -3.1 orf, hypothetical protein (VIMSS) compare
b4458 oxyS -2.4 -1.7 global regulatory RNA (NCBI) compare
b3753 rbsR -2.4 -8.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0142 folK -2.4 -0.9 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b0436 tig -2.4 -3.8 trigger factor (NCBI) compare
b0881 clpS -2.4 -1.4 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b3404 envZ -2.4 -7.7 osmolarity sensor protein (NCBI) compare
b2501 ppk -2.4 -2.8 polyphosphate kinase (NCBI) compare
b2258 b2258 -2.4 -1.2 putative transport/receptor protein (VIMSS) compare
b2570 rseC -2.4 -4.2 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b3932 hslV -2.3 -7.9 ATP-dependent protease peptidase subunit (NCBI) compare
b3075 ebgR -2.3 -3.6 DNA-binding transcriptional repressor (NCBI) compare
b0946 ycbW -2.3 -3.8 orf, hypothetical protein (VIMSS) compare
b1696 ydiP -2.2 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4025 pgi -2.2 -7.1 glucose-6-phosphate isomerase (NCBI) compare
b1677 lpp -2.2 -2.0 murein lipoprotein (NCBI) compare
b3601 mtlR -2.2 -4.7 DNA-binding repressor (NCBI) compare
b2682 ygaZ -2.2 -3.8 predicted transporter (NCBI) compare
b3529 yhjK -2.2 -10.7 predicted diguanylate cyclase (RefSeq) compare
b3423 glpR -2.2 -6.7 DNA-binding transcriptional repressor (NCBI) compare
b3931 hslU -2.1 -9.1 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3443 yrhA -2.1 -1.5 orf, hypothetical protein (VIMSS) compare
b1175 minD -2.1 -2.0 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2329 aroC -2.1 -1.4 chorismate synthase (NCBI) compare
b3790 rffC -2.1 -1.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b0471 ybaB -2.1 -2.0 hypothetical protein (NCBI) compare
b4557 yidD -2.1 -3.1 hypothetical protein (NCBI) compare
b0730 mngR -2.1 -1.4 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b2617 smpA -2.1 -1.5 small membrane protein A (VIMSS) compare
b0052 pdxA -2.1 -6.0 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b2844 yqeF -2.0 -5.9 putative acyltransferase (VIMSS) compare
b1629 rsxC -2.0 -5.3 electron transport complex protein RnfC (NCBI) compare
b3884 yihW -2.0 -3.7 putative DEOR-type transcriptional regulator (VIMSS) compare
b3752 rbsK -2.0 -7.2 ribokinase (NCBI) compare
b2939 yqgB -2.0 -1.4 predicted protein (RefSeq) compare
b0928 aspC -2.0 -3.0 aspartate aminotransferase, PLP-dependent (NCBI) compare
b3424 glpG -2.0 -8.0 predicted intramembrane serine protease (NCBI) compare
b4592 yccB -1.9 -1.7 hypothetical protein (RefSeq) compare
b4203 rplI -1.9 -3.1 50S ribosomal protein L9 (NCBI) compare
b2236 yfaE -1.9 -1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1524 yneH -1.9 -1.0 predicted glutaminase (NCBI) compare
b2643 yfjX -1.9 -2.8 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b1298 ycjL -1.9 -3.4 probable amidotransferase subunit (VIMSS) compare
b3205 yhbJ -1.9 -1.3 hypothetical protein (NCBI) compare
b2037 rfbX -1.9 -6.0 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b1591 b1591 -1.9 -1.4 putative oxidoreductase component (VIMSS) compare
b3107 yhaL -1.8 -1.2 orf, hypothetical protein (VIMSS) compare
b1575 dicB -1.8 -1.7 Qin prophage; cell division inhibition protein (NCBI) compare
b1914 uvrY -1.8 -3.7 response regulator (NCBI) compare
b1104 ycfL -1.8 -1.4 hypothetical protein (NCBI) compare
b3849 trkH -1.8 -2.7 potassium transporter (NCBI) compare
b2741 rpoS -1.7 -2.2 RNA polymerase sigma factor (NCBI) compare
b3438 gntR -1.7 -4.6 DNA-binding transcriptional repressor (NCBI) compare
b2039 rfbA -1.7 -8.4 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b0632 dacA -1.7 -5.1 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1070 flgN -1.7 -1.1 export chaperone for FlgK and FlgL (NCBI) compare
b2513 yfgM -1.7 -3.5 hypothetical protein (NCBI) compare
b4140 b4140 -1.7 -5.5 orf, hypothetical protein (VIMSS) compare
b3157 yhbT -1.7 -2.7 predicted lipid carrier protein (NCBI) compare
b2214 yojL -1.6 -2.9 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b2340 sixA -1.6 -1.6 phosphohistidine phosphatase (NCBI) compare
b4541 yehK -1.6 -3.1 hypothetical protein (NCBI) compare
b2725 hycA -1.6 -2.8 regulator of the transcriptional regulator FhlA (NCBI) compare
b1871 yecP -1.6 -2.8 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2248 yfaX -1.6 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b0035 caiE -1.5 -3.2 possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS) compare
b2058 wcaB -1.5 -1.0 predicted acyl transferase (NCBI) compare
b2376 ypdI -1.5 -2.5 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b4701 sokX -1.5 -0.8 sok-related sRNA, function unknown (RefSeq) compare
b0097 yacA -1.5 -2.6 orf, hypothetical protein (VIMSS) compare
b1582 ynfA -1.5 -1.9 hypothetical protein (NCBI) compare
b0145 dksA -1.5 -1.7 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1567 intK -1.5 -1.6 Qin prophage; predicted protein (RefSeq) compare
b3673 emrD -1.4 -6.3 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b2444 yffM -1.4 -1.2 CPZ-55 prophage; predicted protein (NCBI) compare
b4589 ylcI -1.4 -2.2 hypothetical protein (RefSeq) compare
b0463 acrA -1.4 -5.4 multidrug efflux system (NCBI) compare
b0462 acrB -1.4 -7.0 multidrug efflux system protein (NCBI) compare
b4570 lomR -1.4 -1.3 no description compare
b1926 fliT -1.4 -2.5 predicted chaperone (NCBI) compare
b2433 b2433 -1.4 -1.6 orf, hypothetical protein (VIMSS) compare
b1013 ycdC -1.4 -1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2896 ygfX -1.4 -3.6 hypothetical protein (NCBI) compare
b3933 ftsN -1.4 -5.5 essential cell division protein (NCBI) compare
b2807 ygdD -1.3 -2.0 conserved inner membrane protein (NCBI) compare
b0728 sucC -1.3 -1.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b2366 dsdA -1.3 -3.5 D-serine dehydratase (NCBI) compare
b1278 pgpB -1.3 -1.4 phosphatidylglycerophosphatase B (NCBI) compare
b4522 yciX_1 -1.3 -1.3 hypothetical protein (NCBI) compare
b3228 sspB -1.3 -3.0 ClpXP protease specificity-enhancing factor (NCBI) compare
b3289 rsmB -1.3 -5.3 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b1273 yciN -1.3 -0.9 hypothetical protein (NCBI) compare
b2679 proX -1.3 -2.4 glycine betaine transporter subunit (NCBI) compare
b2024 hisA -1.3 -1.5 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2945 endA -1.3 -2.8 DNA-specific endonuclease I (NCBI) compare
b4698 mgrR -1.3 -1.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1370 insH-5 -1.3 -1.5 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2506 yfgI -1.3 -1.5 hypothetical protein (NCBI) compare
b2435 amiA -1.3 -2.5 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b2215 ompC -1.3 -3.4 outer membrane porin protein C (NCBI) compare
b2504 yfgG -1.3 -0.9 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1482 osmC -1.3 -1.4 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b2362 yfdS -1.3 -2.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2637 yfjT -1.3 -2.3 CP4-57 prophage; predicted protein (NCBI) compare
b3017 sufI -1.2 -5.0 repressor protein for FtsI (NCBI) compare
b0357 yaiN -1.2 -0.8 putative alpha helix chain (VIMSS) compare
b2564 pdxJ -1.2 -2.1 pyridoxal phosphate biosynthetic protein (NCBI) compare
b0055 djlA -1.2 -4.7 Dna-J like membrane chaperone protein (NCBI) compare
b1630 rsxD -1.2 -2.7 electron transport complex protein RnfD (NCBI) compare
b1632 rsxE -1.2 -2.8 NADH-ubiquinone oxidoreductase (NCBI) compare
b1627 rsxA -1.2 -1.6 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b3146 yraL -1.2 -3.8 predicted methyltransferase (NCBI) compare
b3375 yhfR -1.2 -3.4 putative transcriptional regulator (VIMSS) compare
b0661 miaB -1.2 -4.4 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b0563 tfaX -1.2 -3.3 DLP12 prophage; predicted protein (RefSeq) compare
b1358 ydaT -1.2 -0.9 Rac prophage; predicted protein (NCBI) compare
b2156 lysP -1.2 -5.2 lysine transporter (NCBI) compare
b2635 ypjK -1.2 -1.0 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2783 chpR -1.2 -1.1 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b1642 slyA -1.2 -0.8 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2593 yfiH -1.2 -4.2 hypothetical protein (NCBI) compare
b3891 fdhE -1.2 -5.1 formate dehydrogenase accessory protein FdhE (NCBI) compare
b1245 oppC -1.2 -2.2 oligopeptide transporter subunit (NCBI) compare
b4410 ecnA -1.2 -0.9 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1824 yobF -1.2 -1.0 hypothetical protein (NCBI) compare
b3484 yhhI -1.2 -2.1 predicted transposase (NCBI) compare
b1176 minC -1.2 -0.8 septum formation inhibitor (NCBI) compare
b2624 alpA -1.2 -3.6 CP4-57 prophage; DNA-binding transcriptional activator (NCBI) compare
b3890 yiiF -1.2 -1.8 orf, hypothetical protein (VIMSS) compare
b0450 glnK -1.2 -1.9 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b0908 aroA -1.2 -3.1 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b2714 ascG -1.2 -3.3 DNA-binding transcriptional repressor (NCBI) compare
b4630 ykgP -1.2 -1.7 no description compare
b2272 yfbM -1.2 -1.9 hypothetical protein (NCBI) compare
b1108 ycfP -1.2 -1.5 orf, hypothetical protein (VIMSS) compare
b0036 caiD -1.2 -3.0 crotonobetainyl CoA hydratase (RefSeq) compare
b1304 pspA -1.1 -1.6 regulatory protein for phage-shock-protein operon (NCBI) compare
b1954 dsrA -1.1 -0.9 regulatory antisense RNA (NCBI) compare
b1183 umuD -1.1 -1.3 DNA polymerase V, subunit D (NCBI) compare
b3022 b3022 -1.1 -4.5 predicted cyanide hydratase (NCBI) compare
b2786 barA -1.1 -8.2 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b1993 cobU -1.1 -2.2 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI) compare
b2796 sdaC -1.1 -5.3 predicted serine transporter (NCBI) compare
b1021 ycdP -1.1 -2.9 predicted inner membrane protein (NCBI) compare
b1878 flhE -1.1 -2.2 hypothetical protein (NCBI) compare
b0866 ybjQ -1.1 -0.8 hypothetical protein (NCBI) compare
b2295 yfbV -1.1 -2.8 hypothetical protein (NCBI) compare
b0990 cspG -1.1 -0.8 DNA-binding transcriptional regulator (NCBI) compare
b2350 yfdG -1.1 -2.9 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b2993 hybD -1.1 -2.2 predicted maturation element for hydrogenase 2 (NCBI) compare
b1082 flgK -1.1 -3.0 flagellar hook-associated protein K (NCBI) compare
b0034 caiF -1.1 -4.8 transcriptional regulator of cai operon (VIMSS) compare
b1742 b1742 -1.1 -1.5 orf, hypothetical protein (VIMSS) compare
b0280 yagN -1.1 -2.4 CP4-6 prophage; predicted protein (NCBI) compare
b2927 epd -1.1 -3.0 D-erythrose 4-phosphate dehydrogenase (NCBI) compare
b1320 ycjW -1.1 -3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3244 tldD -1.1 -5.0 predicted peptidase (NCBI) compare
b0617 citD -1.1 -2.3 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare


Specific Phenotypes

For 83 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms