Experiment set54IT085 for Escherichia coli BW25113

Compare to:

vcDART vector transformation; liquid outgrowth assay

200 most detrimental genes:

  gene name fitness t score description  
b0191 yaeJ +7.1 19.7 hypothetical protein (NCBI) compare
b3961 oxyR +6.4 4.4 DNA-binding transcriptional dual regulator (NCBI) compare
b0683 fur +6.2 3.6 ferric uptake regulator (NCBI) compare
b3398 yrfF +5.7 15.7 predicted inner membrane protein (NCBI) compare
b3842 rfaH +5.1 18.4 transcriptional activator RfaH (NCBI) compare
b0428 cyoE +5.0 21.7 protoheme IX farnesyltransferase (NCBI) compare
b3806 cyaA +5.0 15.2 adenylate cyclase (NCBI) compare
b0623 cspE +4.9 11.2 cold shock protein E (NCBI) compare
b0432 cyoA +4.9 32.5 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b0429 cyoD +4.8 17.8 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b1835 yebU +4.7 31.5 putative nucleolar proteins (VIMSS) compare
b2906 visC +4.7 19.8 hypothetical protein (NCBI) compare
b0590 fepD +4.6 21.3 iron-enterobactin transporter subunit (NCBI) compare
b0592 fepB +4.5 26.5 iron-enterobactin transporter subunit (NCBI) compare
b3844 fre +4.3 14.9 NAD(P)H-flavin reductase (NCBI) compare
b0588 fepC +4.3 23.5 iron-enterobactin transporter subunit (NCBI) compare
b0431 cyoB +4.2 25.9 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b3493 pitA +4.1 18.5 phosphate transporter, low-affinity (NCBI) compare
b2113 mrp +4.1 21.3 putative ATPase (VIMSS) compare
b0430 cyoC +4.1 16.2 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b3626 rfaJ +4.1 15.2 UDP-D-glucose:(galactosyl)lipopolysaccharide glucosyltransferase (NCBI) compare
b1286 rnb +4.1 6.2 exoribonuclease II (NCBI) compare
b0589 fepG +4.0 21.3 iron-enterobactin transporter subunit (NCBI) compare
b0014 dnaK +4.0 13.2 molecular chaperone DnaK (NCBI) compare
b1235 rssB +3.9 17.8 response regulator of RpoS (NCBI) compare
b4201 priB +3.9 10.8 primosomal replication protein N (NCBI) compare
b2576 srmB +3.9 6.1 ATP-dependent RNA helicase (NCBI) compare
b0720 gltA +3.9 21.9 citrate synthase (NCBI) compare
b2216 rcsD +3.8 12.8 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b3627 rfaI +3.8 18.8 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2528 iscA +3.8 2.6 iron-sulfur cluster assembly protein (NCBI) compare
b3631 rfaG +3.7 26.9 glucosyltransferase I (NCBI) compare
b0427 yajR +3.6 28.6 putative transport protein (VIMSS) compare
b1332 ynaJ +3.6 3.5 predicted inner membrane protein (NCBI) compare
b3181 greA +3.5 19.1 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4234 yjgA +3.5 9.3 hypothetical protein (NCBI) compare
b3206 npr +3.5 15.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b3636 rpmG +3.5 3.9 50S ribosomal protein L33 (NCBI) compare
b4515 ybgT +3.5 8.4 hypothetical protein (NCBI) compare
b3414 gntY +3.5 7.4 predicted gluconate transport associated protein (NCBI) compare
b4402 yjjY +3.4 1.6 hypothetical protein (NCBI) compare
b3630 rfaP +3.4 18.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2829 ptsP +3.4 24.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3628 rfaB +3.4 16.7 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b3183 obgE +3.3 2.1 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2814 metZ +3.3 4.0 tRNA-Met (NCBI) compare
b2688 gshA +3.1 16.4 glutamate--cysteine ligase (NCBI) compare
b4391 yjjK +3.1 22.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3778 rep +3.1 7.3 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2280 nuoJ +3.1 5.2 NADH dehydrogenase subunit J (NCBI) compare
b2218 rcsC +3.1 22.4 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b0759 galE +3.1 16.8 UDP-galactose-4-epimerase (VIMSS) compare
b0438 clpX +3.1 10.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3232 yhcM +3.1 17.6 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b3203 yhbH +3.0 4.8 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b1831 proQ +3.0 11.3 putative solute/DNA competence effector (NCBI) compare
b2946 yggJ +3.0 15.0 orf, hypothetical protein (VIMSS) compare
b3860 dsbA +2.9 18.7 periplasmic protein disulfide isomerase I (NCBI) compare
b1658 purR +2.8 10.2 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3387 dam +2.8 20.2 DNA adenine methylase (NCBI) compare
b2819 recD +2.8 19.1 exonuclease V (RecBCD complex), alpha chain (NCBI) compare
b1612 fumA +2.8 9.1 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b0458 ylaC +2.8 19.2 orf, hypothetical protein (VIMSS) compare
b3620 rfaF +2.7 6.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2587 kgtP +2.5 5.8 alpha-ketoglutarate transporter (NCBI) compare
b4414 tff +2.5 3.0 unknown RNA (NCBI) compare
b3341 rpsG +2.5 1.5 30S ribosomal protein S7 (NCBI) compare
b2502 ppx +2.5 13.1 exopolyphosphatase (NCBI) compare
b3545 proK +2.4 3.2 tRNA-Pro (NCBI) compare
b2436 hemF +2.4 8.5 coproporphyrinogen III oxidase (NCBI) compare
b0464 acrR +2.4 16.6 DNA-binding transcriptional repressor (NCBI) compare
b4371 rsmC +2.4 15.1 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1855 lpxM +2.4 5.9 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3166 truB +2.4 17.0 tRNA pseudouridine synthase B (NCBI) compare
b2096 gatY +2.3 7.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4406 yaeP +2.3 2.1 hypothetical protein (NCBI) compare
b4484 cpxP +2.2 4.0 periplasmic protein combats stress (NCBI) compare
b1989 asnV +2.2 5.8 tRNA-Asn (NCBI) compare
b2659 b2659 +2.2 4.4 orf, hypothetical protein (VIMSS) compare
b1234 rssA +2.2 6.5 conserved protein (RefSeq) compare
b1088 yceD +2.2 7.5 hypothetical protein (NCBI) compare
b2093 gatB +2.2 9.7 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b0216 aspV +2.2 5.2 tRNA-Asp (NCBI) compare
b3071 yqjI +2.1 3.9 predicted transcriptional regulator (NCBI) compare
b1777 b1777 +2.1 6.5 orf, hypothetical protein (VIMSS) compare
b2522 sseB +2.1 16.2 enhanced serine sensitivity (VIMSS) compare
b2584 yfiQ +2.0 9.7 fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI) compare
b3609 secB +2.0 5.0 export protein SecB (NCBI) compare
b3257 yhdT +2.0 5.3 conserved inner membrane protein (NCBI) compare
b1920 fliY +2.0 3.2 cystine transporter subunit (NCBI) compare
b3388 damX +2.0 10.3 hypothetical protein (NCBI) compare
b1236 galU +2.0 2.8 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3210 arcB +2.0 9.8 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b3236 mdh +2.0 9.0 malate dehydrogenase (NCBI) compare
b3009 yghB +1.9 7.6 conserved inner membrane protein (NCBI) compare
b3803 hemX +1.9 11.7 predicted uroporphyrinogen III methylase (NCBI) compare
b4139 aspA +1.9 8.9 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b2908 pepP +1.9 6.4 proline aminopeptidase P II (NCBI) compare
b2754 ygbF +1.9 3.3 orf, hypothetical protein (VIMSS) compare
b1279 yciS +1.9 1.8 conserved inner membrane protein (NCBI) compare
b0467 priC +1.9 4.4 primosomal replication protein N'' (NCBI) compare
b3999 yjaG +1.9 6.2 hypothetical protein (NCBI) compare
b3133 agaV +1.8 2.4 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b1942 fliJ +1.8 3.3 flagellar biosynthesis chaperone (NCBI) compare
b0092 ddlB +1.8 6.7 D-alanylalanine synthetase (NCBI) compare
b0636 ybeA +1.8 8.7 hypothetical protein (NCBI) compare
b1984 asnW +1.8 1.8 tRNA-Asn (NCBI) compare
b0190 yaeQ +1.8 4.8 hypothetical protein (NCBI) compare
b1429 tehA +1.8 1.3 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1329 b1329 +1.8 9.9 putative transport periplasmic protein (VIMSS) compare
b1841 yobA +1.7 2.8 hypothetical protein (NCBI) compare
b3258 panF +1.7 10.4 sodium/pantothenate symporter (VIMSS) compare
b0460 hha +1.7 7.6 modulator of gene expression, with H-NS (NCBI) compare
b3349 slyD +1.7 7.7 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b2285 nuoE +1.7 1.0 NADH dehydrogenase subunit E (NCBI) compare
b1086 rluC +1.7 7.4 23S rRNA pseudouridylate synthase (NCBI) compare
b3784 rfe +1.7 11.1 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1840 yebZ +1.7 8.5 predicted inner membrane protein (NCBI) compare
b0393 rdgC +1.7 10.4 recombination associated protein (NCBI) compare
b2743 pcm +1.7 5.3 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b1143 ymfI +1.6 1.8 orf, hypothetical protein (VIMSS) compare
b0955 ycbZ +1.6 9.6 predicted peptidase (NCBI) compare
b0081 mraZ +1.6 8.9 hypothetical protein (NCBI) compare
b0931 pncB +1.6 5.6 nicotinate phosphoribosyltransferase (NCBI) compare
b1676 pykF +1.6 3.9 pyruvate kinase (NCBI) compare
b3095 yqjA +1.6 6.0 conserved inner membrane protein (NCBI) compare
b3789 rffH +1.6 6.8 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b1281 pyrF +1.6 2.1 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0840 deoR +1.5 3.7 DNA-binding transcriptional repressor (NCBI) compare
b0440 hupB +1.5 4.9 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b1891 flhC +1.5 4.0 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b0869 ybjT +1.5 8.2 putative dTDP-glucose enzyme (VIMSS) compare
b2095 gatZ +1.5 3.8 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b4000 hupA +1.5 5.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1438 ydcQ +1.4 3.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4505 insX +1.4 2.3 predicted IS protein (RefSeq) compare
b3190 yrbA +1.4 4.1 orf, hypothetical protein (VIMSS) compare
b2283 nuoG +1.4 3.3 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b0192 nlpE +1.4 3.4 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b1540 ydfH +1.4 2.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2281 nuoI +1.4 1.5 NADH dehydrogenase subunit I (NCBI) compare
b4370 leuQ +1.4 2.4 tRNA-Leu (NCBI) compare
b3802 hemY +1.4 9.8 predicted protoheme IX synthesis protein (NCBI) compare
b4627 ykfL +1.4 4.1 no description compare
b0082 mraW +1.4 3.7 S-adenosyl-methyltransferase (NCBI) compare
b3751 rbsB +1.4 4.3 D-ribose transporter subunit (NCBI) compare
b1854 pykA +1.4 6.7 pyruvate kinase (NCBI) compare
b3252 yhdA +1.4 9.3 conserved inner membrane protein (NCBI) compare
b2479 gcvR +1.4 3.3 transcriptional regulation of gcv operon (VIMSS) compare
b2286 nuoC +1.4 2.4 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3761 trpT +1.3 1.8 tRNA-Trp (NCBI) compare
b2278 nuoL +1.3 3.6 NADH dehydrogenase subunit L (NCBI) compare
b4646 yrdE +1.3 1.9 no description compare
b0722 sdhD +1.3 2.1 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b1213 ychQ +1.3 3.4 predicted transcriptional regulator (NCBI) compare
b0437 clpP +1.3 2.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0929 ompF +1.3 5.8 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b4426 isrA +1.3 1.6 unknown RNA (NCBI) compare
b4381 deoC +1.3 3.7 deoxyribose-phosphate aldolase (NCBI) compare
b2083 yegZ +1.3 2.8 orf, hypothetical protein (VIMSS) compare
b2282 nuoH +1.3 0.8 NADH dehydrogenase subunit H (NCBI) compare
b2181 yejG +1.3 4.5 hypothetical protein (NCBI) compare
b0473 htpG +1.3 7.4 heat shock protein 90 (NCBI) compare
b3788 rffG +1.3 5.4 dTDP-glucose 4,6-dehydratase (NCBI) compare
b1294 sapA +1.3 4.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2288 nuoA +1.3 2.1 NADH dehydrogenase I chain A (VIMSS) compare
b0741 pal +1.3 1.5 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4577 sgrS +1.3 2.6 small antisense RNA (NCBI) compare
b2277 nuoM +1.3 2.5 NADH dehydrogenase subunit M (NCBI) compare
b1161 ycgX +1.3 1.0 hypothetical protein (NCBI) compare
b4255 yjgD +1.3 3.7 hypothetical protein (NCBI) compare
b0862 artQ +1.3 2.0 arginine transporter subunit (NCBI) compare
b2392 mntH +1.3 6.2 manganese transport protein MntH (NCBI) compare
b0065 yabI +1.3 5.5 conserved inner membrane protein (NCBI) compare
b2804 fucU +1.3 3.3 L-fucose mutarotase (NCBI) compare
b3810 yigA +1.3 7.7 hypothetical protein (NCBI) compare
b2532 yfhQ +1.2 5.7 predicted methyltransferase (NCBI) compare
b0423 thiI +1.2 4.6 thiamine biosynthesis protein ThiI (NCBI) compare
b4369 leuP +1.2 2.8 tRNA-Leu (NCBI) compare
b3197 kdsD +1.2 5.9 D-arabinose 5-phosphate isomerase (NCBI) compare
b4214 cysQ +1.2 8.0 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0121 speE +1.2 3.9 spermidine synthase (NCBI) compare
b4170 mutL +1.2 5.5 DNA mismatch repair protein (NCBI) compare
b3764 yifE +1.2 5.0 hypothetical protein (NCBI) compare
b0122 yacC +1.2 2.3 orf, hypothetical protein (VIMSS) compare
b0447 ybaO +1.2 3.5 putative LRP-like transcriptional regulator (VIMSS) compare
b0088 murD +1.2 1.2 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2538 hcaE +1.2 2.1 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI) compare
b0494 tesA +1.2 3.8 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b1284 yciT +1.2 3.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1231 tyrT +1.2 1.1 tRNA-Tyr (NCBI) compare
b0386 proC +1.2 1.8 pyrroline-5-carboxylate reductase (NCBI) compare
b1439 ydcR +1.1 6.3 fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI) compare
b3196 yrbG +1.1 4.6 predicted calcium/sodium:proton antiporter (NCBI) compare
b3867 hemN +1.1 7.8 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b3766 ilvL +1.1 3.0 ilvG operon leader peptide (NCBI) compare
b4441 glmY +1.1 2.0 sRNA activator of glmS mRNA (RefSeq) compare
b1918 yecS +1.1 2.4 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1847 yebF +1.1 1.5 predicted protein (RefSeq) compare
b2531 iscR +1.1 0.8 DNA-binding transcriptional repressor (NCBI) compare


Specific Phenotypes

For 83 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms