Experiment set54IT080 for Escherichia coli BW25113

Compare to:

mariner vector transformation; agar assay

200 most important genes:

  gene name fitness t score description  
b1830 prc -1.6 -4.6 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1575 dicB -1.5 -2.7 Qin prophage; cell division inhibition protein (NCBI) compare
b1103 ycfF -1.5 -1.8 orf, hypothetical protein (VIMSS) compare
b1961 dcm -1.5 -2.3 DNA cytosine methylase (NCBI) compare
b4520 ymgF -1.4 -1.9 hypothetical protein (NCBI) compare
b2637 yfjT -1.3 -3.6 CP4-57 prophage; predicted protein (NCBI) compare
b4175 hflC -1.3 -7.0 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1583 ynfB -1.3 -4.0 hypothetical protein (NCBI) compare
b1709 btuD -1.3 -1.2 vitamin B12-transporter ATPase (NCBI) compare
b1865 nudB -1.3 -1.1 dATP pyrophosphohydrolase (NCBI) compare
b1620 malI -1.2 -3.4 DNA-binding transcriptional repressor (NCBI) compare
b3466 yhhL -1.2 -5.1 conserved inner membrane protein (NCBI) compare
b1164 ycgZ -1.2 -1.4 hypothetical protein (NCBI) compare
b1503 ydeR -1.2 -3.1 predicted fimbrial-like adhesin protein (NCBI) compare
b1192 ldcA -1.2 -2.6 L,D-carboxypeptidase A (NCBI) compare
b4174 hflK -1.2 -6.4 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b4702 mgtL -1.1 -1.0 regulatory leader peptide for mgtA (NCBI) compare
b1882 cheY -1.1 -0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1936 intG -1.1 -1.0 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4045 yjbJ -1.1 -1.9 predicted stress response protein (NCBI) compare
b0889 lrp -1.1 -3.9 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b4048 yjbM -1.1 -1.7 hypothetical protein (NCBI) compare
b0232 yafN -1.1 -0.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4647 mokA -1.0 -0.9 no description compare
b1765 ydjA -1.0 -1.0 predicted oxidoreductase (NCBI) compare
b0970 yccA -1.0 -2.0 inner membrane protein (NCBI) compare
b3107 yhaL -1.0 -1.5 orf, hypothetical protein (VIMSS) compare
b0059 hepA -1.0 -8.6 ATP-dependent helicase HepA (NCBI) compare
b3912 cpxR -1.0 -5.0 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b4593 ymgI -0.9 -0.6 hypothetical protein (RefSeq) compare
b1255 yciC -0.9 -3.0 hypothetical protein (NCBI) compare
b1361 ydaW -0.9 -0.3 orf, hypothetical protein (VIMSS) compare
b2912 ygfA -0.9 -2.0 putative ligase (VIMSS) compare
b2494 yfgC -0.9 -5.3 predicted peptidase (NCBI) compare
b3555 yiaG -0.9 -2.9 predicted transcriptional regulator (NCBI) compare
b0441 ppiD -0.9 -6.2 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b1818 manY -0.9 -2.5 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0088 murD -0.8 -0.9 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b1422 ydcI -0.8 -3.8 putative transcriptional regulator LYSR-type (VIMSS) compare
b3038 ygiC -0.8 -5.3 predicted enzyme (NCBI) compare
b4232 fbp -0.8 -3.8 fructose-1,6-bisphosphatase (NCBI) compare
b0364 yaiS -0.8 -2.9 orf, hypothetical protein (VIMSS) compare
b1493 gadB -0.8 -0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1368 ynaA -0.8 -2.3 Rac prophage; predicted tail protein (RefSeq) compare
b0418 pgpA -0.8 -3.5 phosphatidylglycerophosphatase A (NCBI) compare
b3119 tdcR -0.8 -1.3 threonine dehydratase operon activator protein (VIMSS) compare
b4509 ylcG -0.7 -2.2 DLP12 prophage; predicted protein (NCBI) compare
b2111 yehD -0.7 -2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b1793 yoaF -0.7 -1.4 conserved outer membrane protein (NCBI) compare
b2698 recX -0.7 -1.5 RecA regulator RecX (NCBI) compare
b4480 hdfR -0.7 -3.5 transcriptional regulator HdfR (NCBI) compare
b2433 b2433 -0.7 -1.6 orf, hypothetical protein (VIMSS) compare
b3611 yibN -0.7 -2.9 predicted rhodanese-related sulfurtransferase (NCBI) compare
b2071 yegJ -0.7 -1.4 hypothetical protein (NCBI) compare
b1524 yneH -0.7 -0.8 predicted glutaminase (NCBI) compare
b4552 yrhC -0.7 -0.6 no description compare
b1332 ynaJ -0.7 -0.5 predicted inner membrane protein (NCBI) compare
b2824 ygdB -0.7 -1.9 orf, hypothetical protein (VIMSS) compare
b1278 pgpB -0.7 -1.7 phosphatidylglycerophosphatase B (NCBI) compare
b3623 rfaK -0.7 -1.4 lipopolysaccharide core biosynthesis (NCBI) compare
b2258 b2258 -0.7 -1.1 putative transport/receptor protein (VIMSS) compare
b0407 yajC -0.7 -1.5 preprotein translocase subunit YajC (NCBI) compare
b0142 folK -0.7 -0.3 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2168 fruK -0.7 -0.5 1-phosphofructokinase (NCBI) compare
b2904 gcvH -0.7 -1.9 glycine cleavage system protein H (NCBI) compare
b1185 dsbB -0.7 -1.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3500 gor -0.6 -0.7 glutathione reductase (NCBI) compare
b2952 yggT -0.6 -3.9 predicted inner membrane protein (NCBI) compare
b3839 tatC -0.6 -1.0 TatABCE protein translocation system subunit (NCBI) compare
b2512 yfgL -0.6 -2.0 protein assembly complex, lipoprotein component (NCBI) compare
b1650 nemA -0.6 -3.4 N-ethylmaleimide reductase, FMN-linked (NCBI) compare
b1734 chbF -0.6 -3.3 cryptic phospho-beta-glucosidase, NAD(P)-binding (NCBI) compare
b4373 rimI -0.6 -2.5 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b2636 b2636 -0.6 -2.9 orf, hypothetical protein (VIMSS) compare
b1111 ycfQ -0.6 -1.8 orf, hypothetical protein (VIMSS) compare
b2362 yfdS -0.6 -2.3 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4128 yjdK -0.6 -0.4 hypothetical protein (NCBI) compare
b1150 ymfR -0.6 -0.8 e14 prophage; predicted protein (NCBI) compare
b4450 arcZ -0.6 -2.7 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0688 pgm -0.6 -1.0 phosphoglucomutase (NCBI) compare
b4630 ykgP -0.6 -1.9 no description compare
b2058 wcaB -0.6 -0.8 predicted acyl transferase (NCBI) compare
b0294 ykgK -0.6 -2.1 predicted regulator (NCBI) compare
b3932 hslV -0.6 -4.3 ATP-dependent protease peptidase subunit (NCBI) compare
b4522 yciX_1 -0.6 -1.4 hypothetical protein (NCBI) compare
b4241 treR -0.6 -4.8 trehalose repressor (NCBI) compare
b3979 thrT -0.6 -0.2 tRNA-Thr (NCBI) compare
b3117 tdcB -0.6 -4.6 threonine dehydratase (NCBI) compare
b2682 ygaZ -0.6 -2.8 predicted transporter (NCBI) compare
b1640 ydhH -0.6 -1.1 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b4557 yidD -0.5 -1.9 hypothetical protein (NCBI) compare
b2372 yfdV -0.5 -3.2 predicted transporter (NCBI) compare
b3875 ompL -0.5 -1.6 predicted outer membrane porin L (NCBI) compare
b1963 b1963 -0.5 -2.0 orf, hypothetical protein (VIMSS) compare
b0791 ybhQ -0.5 -1.7 predicted inner membrane protein (NCBI) compare
b4699 fnrS -0.5 -0.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1179 ycgL -0.5 -1.4 conserved protein (NCBI) compare
b1476 fdnI -0.5 -0.7 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1914 uvrY -0.5 -2.0 response regulator (NCBI) compare
b3792 wzxE -0.5 -0.6 O-antigen translocase (NCBI) compare
b0508 hyi -0.5 -1.4 hydroxypyruvate isomerase (NCBI) compare
b0145 dksA -0.5 -1.2 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1595 ynfL -0.5 -1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2099 yegU -0.5 -1.8 predicted hydrolase (NCBI) compare
b1677 lpp -0.5 -0.9 murein lipoprotein (NCBI) compare
b2939 yqgB -0.5 -0.8 predicted protein (RefSeq) compare
b0143 pcnB -0.5 -2.2 poly(A) polymerase I (VIMSS) compare
b1540 ydfH -0.5 -1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b0881 clpS -0.5 -0.7 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b1160 elbA -0.5 -0.6 hypothetical protein (NCBI) compare
b1577 ydfE -0.5 -2.5 Qin prophage; predicted protein (RefSeq) compare
b3931 hslU -0.5 -3.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3465 yhhF -0.5 -1.6 predicted methyltransferase (NCBI) compare
b2934 cmtB -0.5 -0.5 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3022 b3022 -0.5 -3.3 predicted cyanide hydratase (NCBI) compare
b2266 elaB -0.5 -1.6 hypothetical protein (NCBI) compare
b4481 rffT -0.5 -1.4 4-alpha-L-fucosyltransferase (NCBI) compare
b0610 rnk -0.5 -1.3 nucleoside diphosphate kinase regulator (NCBI) compare
b4535 yniD -0.5 -0.6 predicted protein (RefSeq) compare
b2080 b2080 -0.5 -1.1 orf, hypothetical protein (VIMSS) compare
b2446 yffO -0.5 -1.3 CPZ-55 prophage; predicted protein (NCBI) compare
b4140 b4140 -0.5 -2.5 orf, hypothetical protein (VIMSS) compare
b0653 gltK -0.5 -2.5 glutamate and aspartate transporter subunit (NCBI) compare
b2734 pphB -0.5 -1.9 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b1615 uidC -0.5 -1.7 membrane-associated protein (VIMSS) compare
b3549 tag -0.5 -3.2 3-methyl-adenine DNA glycosylase I, constitutive (NCBI) compare
b1377 ompN -0.4 -1.9 outer membrane pore protein N, non-specific (NCBI) compare
b2707 srlR -0.4 -2.4 DNA-bindng transcriptional repressor (NCBI) compare
b0097 yacA -0.4 -1.1 orf, hypothetical protein (VIMSS) compare
b1960 vsr -0.4 -1.9 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b4487 yjdP -0.4 -2.9 hypothetical protein (NCBI) compare
b1531 marA -0.4 -1.7 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b0338 cynR -0.4 -1.6 DNA-binding transcriptional dual regulator (NCBI) compare
b3962 udhA -0.4 -0.5 putative oxidoreductase (VIMSS) compare
b2903 gcvP -0.4 -3.6 glycine dehydrogenase (NCBI) compare
b4632 ylbI -0.4 -3.3 no description compare
b1878 flhE -0.4 -2.2 hypothetical protein (NCBI) compare
b3723 bglG -0.4 -2.1 transcriptional antiterminator of the bgl operon (NCBI) compare
b4573 insZ -0.4 -1.6 no description compare
b0926 ycbK -0.4 -2.2 hypothetical protein (NCBI) compare
b4567 yjjZ -0.4 -2.2 hypothetical protein (NCBI) compare
b1739 osmE -0.4 -1.0 DNA-binding transcriptional activator (NCBI) compare
b1630 rsxD -0.4 -1.4 electron transport complex protein RnfD (NCBI) compare
b0559 ybcW -0.4 -1.9 DLP12 prophage; predicted protein (NCBI) compare
b3392 yrfA -0.4 -2.9 orf, hypothetical protein (VIMSS) compare
b4644 ypjI -0.4 -2.0 no description compare
b4558 yifL -0.4 -1.3 predicted lipoprotein (NCBI) compare
b4415 hokE -0.4 -1.9 toxic polypeptide, small (NCBI) compare
b1636 pdxY -0.4 -2.5 pyridoxine kinase (NCBI) compare
b3624 rfaZ -0.4 -0.9 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2859 ygeO -0.4 -1.0 hypothetical protein (RefSeq) compare
b2602 yfiL -0.4 -3.0 orf, hypothetical protein (VIMSS) compare
b4631 ykgQ -0.4 -0.9 no description compare
b0617 citD -0.4 -1.3 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b0064 araC -0.4 -2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1685 ydiH -0.4 -0.7 predicted protein (RefSeq) compare
b4596 yciZ -0.4 -0.7 hypothetical protein (RefSeq) compare
b3013 yqhG -0.4 -2.5 hypothetical protein (NCBI) compare
b0080 fruR -0.4 -1.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0852 rimK -0.4 -1.5 ribosomal protein S6 modification protein (NCBI) compare
b0053 surA -0.4 -0.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2683 ygaH -0.4 -2.0 predicted inner membrane protein (NCBI) compare
b1465 narV -0.4 -0.5 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b4171 miaA -0.4 -0.7 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1442 ydcU -0.4 -1.5 predicted spermidine/putrescine transporter subunit (NCBI) compare
b2844 yqeF -0.4 -2.6 putative acyltransferase (VIMSS) compare
b2985 yghS -0.4 -2.6 predicted protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b1219 ychN -0.4 -1.3 hypothetical protein (NCBI) compare
b1175 minD -0.4 -1.0 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3163 nlpI -0.4 -1.5 hypothetical protein (NCBI) compare
b0200 gmhB -0.4 -0.3 hypothetical protein (NCBI) compare
b3562 yiaA -0.4 -3.3 orf, hypothetical protein (VIMSS) compare
b1558 cspF -0.4 -1.0 Qin prophage; cold shock protein (NCBI) compare
b4652 yibU -0.4 -2.6 no description compare
b1605 ydgI -0.4 -2.1 predicted arginine/ornithine antiporter transporter (NCBI) compare
b3199 yrbK -0.4 -1.3 hypothetical protein (NCBI) compare
b0437 clpP -0.4 -1.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0388 aroL -0.4 -1.6 shikimate kinase II (NCBI) compare
b1588 ynfF -0.4 -1.5 oxidoreductase subunit (RefSeq) compare
b4268 idnK -0.4 -2.1 D-gluconate kinase, thermosensitive (NCBI) compare
b4348 hsdS -0.4 -3.0 specificity determinant for hsdM and hsdR (NCBI) compare
b1304 pspA -0.4 -0.9 regulatory protein for phage-shock-protein operon (NCBI) compare
b4413 sokC -0.4 -0.7 regulatory antisense RNA (NCBI) compare
b2510 b2510 -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b0015 dnaJ -0.4 -2.4 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b3870 glnA -0.4 -0.9 glutamine synthetase (NCBI) compare
b2009 sbmC -0.4 -1.7 DNA gyrase inhibitor (NCBI) compare
b2668 ygaP -0.4 -1.9 predicted inner membrane protein with hydrolase activity (NCBI) compare
b0489 ybbK -0.4 -1.9 predicted protease, membrane anchored (NCBI) compare
b1287 yciW -0.4 -1.2 putative oxidoreductase (VIMSS) compare
b2741 rpoS -0.4 -2.1 RNA polymerase sigma factor (NCBI) compare
b4622 ytcA -0.4 -1.9 hypothetical protein (RefSeq) compare
b3973 birA -0.4 -1.2 biotin--protein ligase (NCBI) compare
b3811 xerC -0.4 -0.2 site-specific tyrosine recombinase XerC (NCBI) compare
b2669 stpA -0.4 -3.0 DNA binding protein, nucleoid-associated (NCBI) compare
b4436 ryeC -0.4 -0.7 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b3512 GadE -0.4 -1.7 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0238 gpt -0.4 -1.7 xanthine phosphoribosyltransferase (NCBI) compare
b2633 yfjQ -0.4 -1.9 CP4-57 prophage; predicted protein (NCBI) compare
b4501 torI -0.4 -1.8 response regulator inhibitor for tor operon (NCBI) compare


Specific Phenotypes

For 5 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms