Experiment set54IT080 for Escherichia coli BW25113

Compare to:

mariner vector transformation; agar assay

200 most detrimental genes:

  gene name fitness t score description  
b3961 oxyR +7.1 4.9 DNA-binding transcriptional dual regulator (NCBI) compare
b3414 gntY +5.3 13.0 predicted gluconate transport associated protein (NCBI) compare
b2528 iscA +4.7 4.5 iron-sulfur cluster assembly protein (NCBI) compare
b2281 nuoI +4.6 9.2 NADH dehydrogenase subunit I (NCBI) compare
b2283 nuoG +4.3 18.2 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2284 nuoF +4.2 12.5 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2282 nuoH +4.0 8.2 NADH dehydrogenase subunit H (NCBI) compare
b2280 nuoJ +3.9 15.0 NADH dehydrogenase subunit J (NCBI) compare
b2276 nuoN +3.9 14.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b0683 fur +3.9 2.9 ferric uptake regulator (NCBI) compare
b3844 fre +3.8 13.9 NAD(P)H-flavin reductase (NCBI) compare
b0430 cyoC +3.7 19.7 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2287 nuoB +3.7 9.6 NADH dehydrogenase subunit B (NCBI) compare
b0720 gltA +3.6 24.9 citrate synthase (NCBI) compare
b0428 cyoE +3.6 21.4 protoheme IX farnesyltransferase (NCBI) compare
b0592 fepB +3.5 21.3 iron-enterobactin transporter subunit (NCBI) compare
b2278 nuoL +3.5 19.7 NADH dehydrogenase subunit L (NCBI) compare
b0429 cyoD +3.5 15.1 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b2286 nuoC +3.4 10.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2017 yefM +3.4 2.9 orf, hypothetical protein (VIMSS) compare
b0588 fepC +3.4 19.0 iron-enterobactin transporter subunit (NCBI) compare
b0590 fepD +3.4 17.2 iron-enterobactin transporter subunit (NCBI) compare
b0432 cyoA +3.3 22.8 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b2277 nuoM +3.3 13.1 NADH dehydrogenase subunit M (NCBI) compare
b2285 nuoE +3.3 4.3 NADH dehydrogenase subunit E (NCBI) compare
b2288 nuoA +3.3 11.6 NADH dehydrogenase I chain A (VIMSS) compare
b0589 fepG +3.2 18.6 iron-enterobactin transporter subunit (NCBI) compare
b4402 yjjY +3.2 2.8 hypothetical protein (NCBI) compare
b2906 visC +3.2 17.6 hypothetical protein (NCBI) compare
b3778 rep +3.0 11.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b0722 sdhD +3.0 8.6 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0431 cyoB +3.0 21.3 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b2479 gcvR +2.9 9.4 transcriptional regulation of gcv operon (VIMSS) compare
b3871 bipA +2.9 18.4 GTP-binding protein (NCBI) compare
b1236 galU +2.7 8.1 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3398 yrfF +2.7 8.7 predicted inner membrane protein (NCBI) compare
b2531 iscR +2.6 3.1 DNA-binding transcriptional repressor (NCBI) compare
b3631 rfaG +2.6 21.0 glucosyltransferase I (NCBI) compare
b0723 sdhA +2.6 14.4 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3236 mdh +2.5 13.9 malate dehydrogenase (NCBI) compare
b0724 sdhB +2.5 9.6 succinate dehydrogenase, FeS subunit (NCBI) compare
b3630 rfaP +2.5 15.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0427 yajR +2.4 18.1 putative transport protein (VIMSS) compare
b3390 aroK +2.4 2.1 shikimate kinase I (NCBI) compare
b3162 deaD +2.4 7.0 inducible ATP-independent RNA helicase (VIMSS) compare
b4414 tff +2.4 3.0 unknown RNA (NCBI) compare
b3237 argR +2.4 7.1 arginine repressor (NCBI) compare
b2784 relA +2.4 13.8 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2576 srmB +2.4 4.7 ATP-dependent RNA helicase (NCBI) compare
b2814 metZ +2.4 3.6 tRNA-Met (NCBI) compare
b3842 rfaH +2.4 10.3 transcriptional activator RfaH (NCBI) compare
b0014 dnaK +2.3 4.3 molecular chaperone DnaK (NCBI) compare
b1658 purR +2.2 10.1 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0623 cspE +2.2 3.4 cold shock protein E (NCBI) compare
b3636 rpmG +2.2 4.4 50S ribosomal protein L33 (NCBI) compare
b0721 sdhC +2.1 6.1 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b0222 lpcA +2.1 3.0 phosphoheptose isomerase (NCBI) compare
b3493 pitA +2.1 10.8 phosphate transporter, low-affinity (NCBI) compare
b0585 fes +2.0 8.0 enterobactin/ferric enterobactin esterase (NCBI) compare
b4515 ybgT +2.0 5.9 hypothetical protein (NCBI) compare
b4201 priB +1.9 5.0 primosomal replication protein N (NCBI) compare
b0191 yaeJ +1.9 5.4 hypothetical protein (NCBI) compare
b2279 nuoK +1.9 2.4 NADH dehydrogenase subunit K (NCBI) compare
b4234 yjgA +1.8 6.8 hypothetical protein (NCBI) compare
b0729 sucD +1.8 3.5 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2498 upp +1.8 2.5 uracil phosphoribosyltransferase (VIMSS) compare
b2895 fldB +1.7 4.9 flavodoxin 2 (NCBI) compare
b1161 ycgX +1.7 2.0 hypothetical protein (NCBI) compare
b3977 tyrU +1.7 1.7 tRNA-Tyr (NCBI) compare
b3761 trpT +1.7 3.0 tRNA-Trp (NCBI) compare
b3806 cyaA +1.7 6.9 adenylate cyclase (NCBI) compare
b2113 mrp +1.7 8.0 putative ATPase (VIMSS) compare
b3545 proK +1.6 2.9 tRNA-Pro (NCBI) compare
b1730 ydjO +1.6 2.5 predicted protein (RefSeq) compare
b0888 trxB +1.6 4.8 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b4139 aspA +1.6 9.4 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b1235 rssB +1.6 8.2 response regulator of RpoS (NCBI) compare
b4685 yrbN +1.5 3.6 expressed protein (RefSeq) compare
b3609 secB +1.5 4.9 export protein SecB (NCBI) compare
b1346 ydaQ +1.5 3.1 orf, hypothetical protein (VIMSS) compare
b3183 obgE +1.5 0.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3164 pnp +1.5 3.7 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3232 yhcM +1.4 10.2 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b0082 mraW +1.4 3.6 S-adenosyl-methyltransferase (NCBI) compare
b0471 ybaB +1.4 3.7 hypothetical protein (NCBI) compare
b3807 cyaY +1.4 7.5 frataxin-like protein (NCBI) compare
b0728 sucC +1.4 5.3 succinyl-CoA synthetase subunit beta (NCBI) compare
b1095 fabF +1.4 5.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1855 lpxM +1.4 4.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3229 sspA +1.4 2.1 stringent starvation protein A (NCBI) compare
b3911 cpxA +1.4 5.1 two-component sensor protein (NCBI) compare
b3341 rpsG +1.3 1.6 30S ribosomal protein S7 (NCBI) compare
b4208 cycA +1.3 9.3 D-alanine/D-serine/glycine transporter (NCBI) compare
b1252 tonB +1.3 3.0 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1835 yebU +1.3 7.3 putative nucleolar proteins (VIMSS) compare
b1612 fumA +1.2 5.0 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b0784 moaD +1.2 3.8 molybdopterin synthase, small subunit (NCBI) compare
b0216 aspV +1.2 4.3 tRNA-Asp (NCBI) compare
b3620 rfaF +1.2 4.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2293 yfbT +1.2 3.2 putative phosphatase (VIMSS) compare
b1281 pyrF +1.2 3.5 orotidine 5'-phosphate decarboxylase (NCBI) compare
b1712 ihfA +1.2 1.1 integration host factor subunit alpha (NCBI) compare
b1090 plsX +1.2 4.5 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1860 ruvB +1.2 0.7 Holliday junction DNA helicase B (NCBI) compare
b3616 tdh +1.1 5.2 L-threonine 3-dehydrogenase (NCBI) compare
b0605 ahpC +1.1 2.1 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1286 rnb +1.1 2.4 exoribonuclease II (NCBI) compare
b1347 ydaC +1.1 0.6 Rac prophage; predicted protein (NCBI) compare
b1551 b1551 +1.1 0.6 orf, hypothetical protein (VIMSS) compare
b3190 yrbA +1.1 5.3 orf, hypothetical protein (VIMSS) compare
b2216 rcsD +1.1 7.6 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b2897 ygfY +1.1 1.9 hypothetical protein (NCBI) compare
b0019 nhaA +1.1 7.3 pH-dependent sodium/proton antiporter (NCBI) compare
b2830 nudH +1.1 0.9 dinucleoside polyphosphate hydrolase (NCBI) compare
b3052 rfaE +1.1 2.5 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3198 kdsC +1.1 3.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b0458 ylaC +1.1 5.4 orf, hypothetical protein (VIMSS) compare
b1025 ycdT +1.0 2.4 predicted diguanylate cyclase (NCBI) compare
b2330 yfcB +1.0 1.1 putative adenine-specific methylase (VIMSS) compare
b3387 dam +1.0 7.8 DNA adenine methylase (NCBI) compare
b3210 arcB +1.0 6.1 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b0564 appY +1.0 1.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3204 ptsN +1.0 3.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1600 mdtJ +1.0 2.7 multidrug efflux system transporter (NCBI) compare
b1506 yneL +1.0 1.2 predicted transcriptional regulator (NCBI) compare
b1279 yciS +1.0 1.5 conserved inner membrane protein (NCBI) compare
b3766 ilvL +1.0 3.7 ilvG operon leader peptide (NCBI) compare
b4243 yjgF +1.0 4.0 orf, hypothetical protein (VIMSS) compare
b3257 yhdT +1.0 3.5 conserved inner membrane protein (NCBI) compare
b0726 sucA +1.0 1.0 alpha-ketoglutarate decarboxylase (NCBI) compare
b4578 insP +1.0 3.2 no description compare
b2688 gshA +0.9 5.6 glutamate--cysteine ligase (NCBI) compare
b2699 recA +0.9 0.6 recombinase A (NCBI) compare
b0438 clpX +0.9 4.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4585 sroB +0.9 1.5 Novel sRNA, function unknown (RefSeq) compare
b0469 apt +0.9 5.4 adenine phosphoribosyltransferase (NCBI) compare
b1921 fliZ +0.9 2.7 orf, hypothetical protein (VIMSS) compare
b3999 yjaG +0.9 4.1 hypothetical protein (NCBI) compare
b1989 asnV +0.9 2.9 tRNA-Asn (NCBI) compare
b0912 ihfB +0.9 2.2 integration host factor subunit beta (NCBI) compare
b4180 rlmB +0.9 5.1 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b0237 pepD +0.9 4.9 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b0741 pal +0.9 1.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2908 pepP +0.9 4.7 proline aminopeptidase P II (NCBI) compare
b2569 lepA +0.9 2.3 GTP-binding protein LepA (NCBI) compare
b2518 ndk +0.9 1.7 nucleoside diphosphate kinase (NCBI) compare
b3260 dusB +0.8 3.8 tRNA-dihydrouridine synthase B (NCBI) compare
b1840 yebZ +0.8 4.3 predicted inner membrane protein (NCBI) compare
b2218 rcsC +0.8 6.4 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b1572 ydfB +0.8 1.6 orf, hypothetical protein (VIMSS) compare
b0685 ybfE +0.8 2.3 orf, hypothetical protein (VIMSS) compare
b4407 thiS +0.8 2.1 sulfur carrier protein ThiS (NCBI) compare
b2553 glnB +0.8 2.7 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1074 flgC +0.8 2.3 flagellar basal-body rod protein C (NCBI) compare
b2565 recO +0.8 2.9 DNA repair protein RecO (NCBI) compare
b4178 yjeB +0.8 3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2516 yfgA +0.8 3.6 hypothetical protein (NCBI) compare
b2237 inaA +0.8 1.8 hypothetical protein (NCBI) compare
b2584 yfiQ +0.8 5.8 fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI) compare
b4018 iclR +0.8 4.6 repressor of aceBA operon (VIMSS) compare
b3005 exbD +0.8 4.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3425 glpE +0.8 4.1 thiosulfate sulfurtransferase (NCBI) compare
b0092 ddlB +0.8 2.6 D-alanylalanine synthetase (NCBI) compare
b4484 cpxP +0.8 4.3 periplasmic protein combats stress (NCBI) compare
b2436 hemF +0.7 2.9 coproporphyrinogen III oxidase (NCBI) compare
b2579 yfiD +0.7 1.7 pyruvate formate lyase subunit (NCBI) compare
b0464 acrR +0.7 5.7 DNA-binding transcriptional repressor (NCBI) compare
b0051 ksgA +0.7 2.0 dimethyladenosine transferase (NCBI) compare
b0450 glnK +0.7 1.3 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b1382 ynbE +0.7 1.5 predicted lipoprotein (NCBI) compare
b0949 uup +0.7 3.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2564 pdxJ +0.7 2.3 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1684 sufA +0.7 1.6 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1162 ycgE +0.7 3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1573 ydfC +0.7 1.0 Qin prophage; predicted protein (RefSeq) compare
b4203 rplI +0.7 3.6 50S ribosomal protein L9 (NCBI) compare
b4497 yeeL +0.7 1.1 no description compare
b2082 ogrK +0.7 2.9 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b2007 yeeX +0.7 1.7 conserved protein (RefSeq) compare
b3642 pyrE +0.7 2.7 orotate phosphoribosyltransferase (NCBI) compare
b4066 yjcF +0.7 0.4 hypothetical protein (NCBI) compare
b4391 yjjK +0.7 4.7 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1690 ydiM +0.7 2.3 predicted transporter (NCBI) compare
b3600 mtlD +0.7 3.3 mannitol-1-phosphate 5-dehydrogenase (NCBI) compare
b4371 rsmC +0.7 4.9 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b3794 rffM +0.7 2.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1566 flxA +0.7 2.1 Qin prophage; predicted protein (NCBI) compare
b4101 phnG +0.7 2.7 carbon-phosphorus lyase complex subunit (NCBI) compare
b3191 yrbB +0.7 4.8 orf, hypothetical protein (VIMSS) compare
b3349 slyD +0.7 3.6 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b1049 mdoH +0.7 4.5 glucosyltransferase MdoH (NCBI) compare
b1187 fadR +0.7 2.4 fatty acid metabolism regulator (NCBI) compare
b1758 b1758 +0.7 1.1 putative cytochrome oxidase (VIMSS) compare
b4513 kdpF +0.7 1.5 potassium ion accessory transporter subunit (NCBI) compare
b1151 ymfO +0.7 2.7 e14 prophage; conserved protein (RefSeq) compare
b0494 tesA +0.7 2.9 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b1480 sra +0.7 1.1 30S ribosomal subunit protein S22 (NCBI) compare
b2502 ppx +0.7 3.9 exopolyphosphatase (NCBI) compare
b0785 moaE +0.7 4.1 molybdopterin synthase, large subunit (NCBI) compare
b0764 modB +0.7 3.0 molybdate ABC transporter permease protein (NCBI) compare


Specific Phenotypes

For 5 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms