Experiment set54IT080 for Escherichia coli BW25113

mariner vector transformation; agar assay

Group: plasmid transformation
Media: LB + Kanamycin sulfate (0.05 mg/ml) + Gentamicin sulfate salt (0.05 mg/ml)
Culturing: Keio_ML9a, Bioassay Dish, Aerobic, at 30 (C), (Solid)
By: Rachel Rovinsky on 3/30/22
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 5 genes in this experiment

For plasmid transformation Kanamycin sulfate in Escherichia coli BW25113

For plasmid transformation Kanamycin sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Proteolysis in bacteria, ATP-dependent	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway