Experiment set53IT087 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 1.98 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b1244 oppB +2.2 14.2 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1095 fabF +2.1 8.2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1247 oppF +2.0 10.8 oligopeptide transporter subunit (NCBI) compare
b2469 narQ +2.0 5.0 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b1243 oppA +1.9 11.4 oligopeptide transporter subunit (NCBI) compare
b1246 oppD +1.9 11.5 oligopeptide transporter ATP-binding component (NCBI) compare
b1245 oppC +1.8 8.2 oligopeptide transporter subunit (NCBI) compare
b3911 cpxA +1.6 11.7 two-component sensor protein (NCBI) compare
b2684 mprA +1.6 4.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3390 aroK +1.2 2.9 shikimate kinase I (NCBI) compare
b2642 yfjW +1.2 2.4 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1058 yceO +1.1 0.8 hypothetical protein (NCBI) compare
b1936 intG +1.1 1.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1607 ydgC +1.1 3.4 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4133 cadC +1.1 9.9 DNA-binding transcriptional activator (NCBI) compare
b3979 thrT +1.0 0.8 tRNA-Thr (NCBI) compare
b0464 acrR +1.0 7.6 DNA-binding transcriptional repressor (NCBI) compare
b4585 sroB +1.0 2.6 Novel sRNA, function unknown (RefSeq) compare
b4313 fimE +0.9 7.7 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2078 baeS +0.9 2.3 sensory histidine kinase in two-component regulatory system with BaeR (NCBI) compare
b2217 rcsB +0.9 6.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1627 rsxA +0.8 4.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1182 hlyE +0.8 0.7 hemolysin E (VIMSS) compare
b3146 yraL +0.7 5.6 predicted methyltransferase (NCBI) compare
b1693 aroD +0.7 3.3 3-dehydroquinate dehydratase (NCBI) compare
b1236 galU +0.7 2.1 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2181 yejG +0.7 4.2 hypothetical protein (NCBI) compare
b0232 yafN +0.7 0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2531 iscR +0.7 1.6 DNA-binding transcriptional repressor (NCBI) compare
b4426 isrA +0.7 2.5 unknown RNA (NCBI) compare
b1049 mdoH +0.7 5.5 glucosyltransferase MdoH (NCBI) compare
b2179 yejE +0.7 4.1 predicted oligopeptide transporter subunit (NCBI) compare
b1954 dsrA +0.7 2.5 regulatory antisense RNA (NCBI) compare
b1628 rsxB +0.7 2.3 electron transport complex protein RnfB (NCBI) compare
b4383 deoB +0.7 5.2 phosphopentomutase (NCBI) compare
b2189 proL +0.7 2.4 tRNA-Pro (NCBI) compare
b1505 ydeT +0.7 1.3 hypothetical protein (RefSeq) compare
b2502 ppx +0.7 5.1 exopolyphosphatase (NCBI) compare
b1874 cutC +0.7 0.9 copper homeostasis protein (NCBI) compare
b1253 yciA +0.7 1.7 predicted hydrolase (NCBI) compare
b1001 yccE +0.7 0.4 hypothetical protein (NCBI) compare
b1497 b1497 +0.6 0.3 putative enzyme (VIMSS) compare
b1159 mcrA +0.6 1.2 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b0929 ompF +0.6 4.2 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b3397 nudE +0.6 5.3 ADP-ribose diphosphatase (NCBI) compare
b1600 mdtJ +0.6 1.5 multidrug efflux system transporter (NCBI) compare
b3194 yrbE +0.6 4.7 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3238 yhcN +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b1576 ydfD +0.6 0.3 Qin prophage; predicted protein (NCBI) compare
b1164 ycgZ +0.6 1.5 hypothetical protein (NCBI) compare
b3429 glgA +0.6 5.2 glycogen synthase (NCBI) compare
b2934 cmtB +0.6 0.8 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1632 rsxE +0.6 4.4 NADH-ubiquinone oxidoreductase (NCBI) compare
b2346 vacJ +0.6 4.4 predicted lipoprotein (NCBI) compare
b1690 ydiM +0.6 1.9 predicted transporter (NCBI) compare
b3755 yieP +0.6 3.6 predicted transcriptional regulator (NCBI) compare
b1709 btuD +0.6 0.8 vitamin B12-transporter ATPase (NCBI) compare
b2177 yejA +0.6 4.7 orf, hypothetical protein (VIMSS) compare
b2180 yejF +0.6 4.5 fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI) compare
b1630 rsxD +0.6 3.4 electron transport complex protein RnfD (NCBI) compare
b2741 rpoS +0.6 4.6 RNA polymerase sigma factor (NCBI) compare
b4246 pyrL +0.6 1.2 pyrBI operon leader peptide (NCBI) compare
b1347 ydaC +0.6 0.3 Rac prophage; predicted protein (NCBI) compare
b3206 npr +0.6 3.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1824 yobF +0.6 1.7 hypothetical protein (NCBI) compare
b1048 mdoG +0.6 3.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b3107 yhaL +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b3779 gpp +0.6 5.2 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b1545 pinQ +0.6 0.2 Qin prophage; predicted site-specific recombinase (NCBI) compare
b2659 b2659 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b2911 ssrS +0.6 1.3 6S regulatory RNA (NCBI) compare
b1493 gadB +0.6 0.9 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b3443 yrhA +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b1670 ydhU +0.5 2.6 predicted cytochrome (NCBI) compare
b1823 cspC +0.5 2.1 stress protein, member of the CspA-family (NCBI) compare
b2618 b2618 +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +0.5 0.5 predicted inner membrane protein (NCBI) compare
b0858 ybjO +0.5 2.2 predicted inner membrane protein (NCBI) compare
b1499 ydeO +0.5 0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1765 ydjA +0.5 0.7 predicted oxidoreductase (NCBI) compare
b1671 ydhX +0.5 2.0 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b0782 moaB +0.5 1.6 molybdopterin biosynthesis protein B (NCBI) compare
b4041 plsB +0.5 1.8 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1257 yciE +0.5 0.2 hypothetical protein (NCBI) compare
b2734 pphB +0.5 2.7 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b1075 flgD +0.5 2.3 flagellar basal body rod modification protein D (NCBI) compare
b1284 yciT +0.5 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2012 yeeD +0.5 1.9 hypothetical protein (NCBI) compare
b2136 yohD +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b3753 rbsR +0.5 4.2 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b1726 yniB +0.5 1.3 predicted inner membrane protein (NCBI) compare
b3884 yihW +0.5 3.0 putative DEOR-type transcriptional regulator (VIMSS) compare
b4655 ythA +0.5 1.2 expressed protein (RefSeq) compare
b2178 yejB +0.5 2.9 predicted oligopeptide transporter subunit (NCBI) compare
b3009 yghB +0.5 3.2 conserved inner membrane protein (NCBI) compare
b3495 uspA +0.5 4.2 universal stress global response regulator (NCBI) compare
b1363 trkG +0.5 0.3 Rac prophage; potassium transporter subunit (NCBI) compare
b2450 yffS +0.5 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b0661 miaB +0.5 4.0 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1480 sra +0.5 0.7 30S ribosomal subunit protein S22 (NCBI) compare
b1492 gadC +0.4 3.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1306 pspC +0.4 1.3 DNA-binding transcriptional activator (NCBI) compare
b1167 ymgC +0.4 0.2 hypothetical protein (NCBI) compare
b1409 ynbB +0.4 0.6 predicted CDP-diglyceride synthase (NCBI) compare
b2229 b2229 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b1704 aroH +0.4 2.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) compare
b2214 yojL +0.4 2.7 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b4618 tisB +0.4 3.1 lexA-regulated toxic peptide (RefSeq) compare
b0955 ycbZ +0.4 3.2 predicted peptidase (NCBI) compare
b2637 yfjT +0.4 1.3 CP4-57 prophage; predicted protein (NCBI) compare
b2528 iscA +0.4 0.7 iron-sulfur cluster assembly protein (NCBI) compare
b3281 aroE +0.4 3.4 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2145 yeiS +0.4 2.4 predicted inner membrane protein (NCBI) compare
b0736 ybgC +0.4 2.0 predicted acyl-CoA thioesterase (NCBI) compare
b0806 ybiM +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1400 paaY +0.4 1.1 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2007 yeeX +0.4 0.8 conserved protein (RefSeq) compare
b1132 hflD +0.4 2.0 hypothetical protein (NCBI) compare
b0218 yafU +0.4 1.7 predicted inner membrane protein (RefSeq) compare
b2960 yggH +0.4 2.6 tRNA(m7G46)-methyltransferase (NCBI) compare
b1861 ruvA +0.4 0.3 Holliday junction DNA helicase motor protein (NCBI) compare
b3192 yrbC +0.4 3.4 predicted ABC-type organic solvent transporter (NCBI) compare
b2570 rseC +0.4 2.5 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b1205 ychH +0.4 1.4 predicted inner membrane protein (NCBI) compare
b1007 b1007 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1648 b1648 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1951 rcsA +0.4 0.9 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2377 yfdY +0.4 1.7 predicted inner membrane protein (NCBI) compare
b1502 ydeQ +0.4 1.1 predicted fimbrial-like adhesin protein (NCBI) compare
b0803 ybiI +0.4 1.8 hypothetical protein (NCBI) compare
b1407 ydbD +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b1983 yeeN +0.4 2.1 hypothetical protein (NCBI) compare
b1490 yddV +0.4 2.1 predicted diguanylate cyclase (RefSeq) compare
b4631 ykgQ +0.4 1.3 no description compare
b0755 gpmA +0.4 2.7 phosphoglyceromutase (NCBI) compare
b3341 rpsG +0.4 0.8 30S ribosomal protein S7 (NCBI) compare
b1727 yniC +0.4 1.2 predicted hydrolase (NCBI) compare
b1712 ihfA +0.4 0.2 integration host factor subunit alpha (NCBI) compare
b0065 yabI +0.4 2.1 conserved inner membrane protein (NCBI) compare
b1591 b1591 +0.4 1.3 putative oxidoreductase component (VIMSS) compare
b3611 yibN +0.4 2.3 predicted rhodanese-related sulfurtransferase (NCBI) compare
b1429 tehA +0.4 0.7 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b3816 corA +0.4 3.0 magnesium/nickel/cobalt transporter (NCBI) compare
b1760 ynjH +0.4 2.2 hypothetical protein (NCBI) compare
b1242 ychE +0.4 2.3 predicted inner membrane protein (NCBI) compare
b3466 yhhL +0.4 2.0 conserved inner membrane protein (NCBI) compare
b1848 yebG +0.4 0.9 conserved protein regulated by LexA (NCBI) compare
b3432 glgB +0.4 3.2 glycogen branching enzyme (NCBI) compare
b0001 thrL +0.4 1.9 thr operon leader peptide (NCBI) compare
b1642 slyA +0.4 1.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2571 rseB +0.4 2.5 periplasmic negative regulator of sigmaE (NCBI) compare
b1263 trpD +0.4 1.5 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0377 sbmA +0.4 2.8 predicted transporter (NCBI) compare
b0530 sfmA +0.3 1.6 putative fimbrial-like protein (VIMSS) compare
b1038 csgF +0.3 1.6 predicted transport protein (NCBI) compare
b1368 ynaA +0.3 1.2 Rac prophage; predicted tail protein (RefSeq) compare
b4222 ytfP +0.3 1.6 hypothetical protein (NCBI) compare
b2479 gcvR +0.3 2.0 transcriptional regulation of gcv operon (VIMSS) compare
b4557 yidD +0.3 1.8 hypothetical protein (NCBI) compare
b0759 galE +0.3 2.6 UDP-galactose-4-epimerase (VIMSS) compare
b4128 yjdK +0.3 0.3 hypothetical protein (NCBI) compare
b1733 chbG +0.3 1.5 hypothetical protein (NCBI) compare
b0965 b0965 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b2200 ccmB +0.3 2.1 heme exporter subunit (NCBI) compare
b2792 yqcC +0.3 1.5 hypothetical protein (NCBI) compare
b3195 yrbF +0.3 2.8 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1583 ynfB +0.3 1.0 hypothetical protein (NCBI) compare
b4520 ymgF +0.3 0.7 hypothetical protein (NCBI) compare
b1325 ycjG +0.3 1.7 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b4559 yjdO +0.3 1.7 hypothetical protein (NCBI) compare
b1950 fliR +0.3 1.4 flagellar biosynthesis protein R (NCBI) compare
b3812 yigB +0.3 2.2 predicted hydrolase (NCBI) compare
b1579 intQ +0.3 1.2 Qin prophage; predicted defective integrase (RefSeq) compare
b4406 yaeP +0.3 1.3 hypothetical protein (NCBI) compare
b1689 b1689 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b3858 yihD +0.3 1.2 hypothetical protein (NCBI) compare
b1518 lsrG +0.3 1.1 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b0866 ybjQ +0.3 0.8 hypothetical protein (NCBI) compare
b3071 yqjI +0.3 1.8 predicted transcriptional regulator (NCBI) compare
b4596 yciZ +0.3 1.2 hypothetical protein (RefSeq) compare
b1949 fliQ +0.3 1.5 flagellar biosynthesis protein Q (NCBI) compare
b1168 b1168 +0.3 1.7 putative proteases (VIMSS) compare
b3191 yrbB +0.3 2.5 orf, hypothetical protein (VIMSS) compare
b0753 ybgS +0.3 1.9 hypothetical protein (NCBI) compare
b1618 uidR +0.3 1.8 DNA-binding transcriptional repressor (NCBI) compare
b4214 cysQ +0.3 2.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0014 dnaK +0.3 1.2 molecular chaperone DnaK (NCBI) compare
b3766 ilvL +0.3 1.3 ilvG operon leader peptide (NCBI) compare
b1969 yedW +0.3 1.6 putative 2-component transcriptional regulator (VIMSS) compare
b0959 yccR +0.3 2.1 hypothetical protein (NCBI) compare
b3802 hemY +0.3 2.7 predicted protoheme IX synthesis protein (NCBI) compare
b2586 yfiM +0.3 1.6 predicted protein (RefSeq) compare
b1062 pyrC +0.3 1.3 dihydroorotase (NCBI) compare
b3672 ivbL +0.3 2.0 ilvB operon leader peptide (NCBI) compare
b2055 wcaE +0.3 0.1 predicted glycosyl transferase (NCBI) compare
b0213 yafS +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b3609 secB +0.3 0.8 export protein SecB (NCBI) compare
b0294 ykgK +0.3 1.4 predicted regulator (NCBI) compare
b0104 guaC +0.3 2.5 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b1309 ycjM +0.3 1.5 putative polysaccharide hydrolase (VIMSS) compare


Specific Phenotypes

For 5 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms