Experiment set53IT085 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.1 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b1936 intG +1.5 2.3 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1085 b1085 +1.5 4.5 orf, hypothetical protein (VIMSS) compare
b1246 oppD +1.2 8.7 oligopeptide transporter ATP-binding component (NCBI) compare
b1245 oppC +1.1 8.2 oligopeptide transporter subunit (NCBI) compare
b1244 oppB +1.1 7.7 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1247 oppF +1.1 6.0 oligopeptide transporter subunit (NCBI) compare
b1243 oppA +1.1 7.6 oligopeptide transporter subunit (NCBI) compare
b3261 fis +0.9 2.6 DNA-binding protein Fis (NCBI) compare
b1765 ydjA +0.9 1.4 predicted oxidoreductase (NCBI) compare
b0929 ompF +0.8 6.5 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2007 yeeX +0.8 1.8 conserved protein (RefSeq) compare
b2114 metG +0.8 2.6 methionyl-tRNA synthetase (NCBI) compare
b1961 dcm +0.7 1.7 DNA cytosine methylase (NCBI) compare
b4585 sroB +0.7 1.8 Novel sRNA, function unknown (RefSeq) compare
b1591 b1591 +0.7 2.0 putative oxidoreductase component (VIMSS) compare
b4603 rseX +0.7 0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2688 gshA +0.7 4.9 glutamate--cysteine ligase (NCBI) compare
b0955 ycbZ +0.7 5.5 predicted peptidase (NCBI) compare
b2947 gshB +0.7 2.6 glutathione synthetase (NCBI) compare
b4545 ypdJ +0.7 2.4 no description compare
b1257 yciE +0.6 0.4 hypothetical protein (NCBI) compare
b0464 acrR +0.6 5.4 DNA-binding transcriptional repressor (NCBI) compare
b1583 ynfB +0.6 3.0 hypothetical protein (NCBI) compare
b0864 artP +0.6 2.7 arginine transporter subunit (NCBI) compare
b1882 cheY +0.6 0.8 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4426 isrA +0.6 2.4 unknown RNA (NCBI) compare
b3778 rep +0.6 3.9 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b0232 yafN +0.6 0.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1090 plsX +0.6 2.3 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4133 cadC +0.6 5.4 DNA-binding transcriptional activator (NCBI) compare
b1818 manY +0.6 1.3 mannose-specific enzyme IIC component of PTS (NCBI) compare
b2659 b2659 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b0858 ybjO +0.6 2.5 predicted inner membrane protein (NCBI) compare
b2346 vacJ +0.6 4.5 predicted lipoprotein (NCBI) compare
b0802 ybiJ +0.6 2.8 hypothetical protein (NCBI) compare
b3443 yrhA +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b1613 manA +0.6 1.4 mannose-6-phosphate isomerase (VIMSS) compare
b0310 ykgH +0.6 1.4 predicted inner membrane protein (NCBI) compare
b2217 rcsB +0.6 4.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3146 yraL +0.5 4.3 predicted methyltransferase (NCBI) compare
b0065 yabI +0.5 3.6 conserved inner membrane protein (NCBI) compare
b3192 yrbC +0.5 4.6 predicted ABC-type organic solvent transporter (NCBI) compare
b1179 ycgL +0.5 1.3 conserved protein (NCBI) compare
b1628 rsxB +0.5 1.7 electron transport complex protein RnfB (NCBI) compare
b3979 thrT +0.5 0.4 tRNA-Thr (NCBI) compare
b4163 glyV +0.5 1.1 tRNA-Gly (NCBI) compare
b1642 slyA +0.5 2.3 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4203 rplI +0.5 2.8 50S ribosomal protein L9 (NCBI) compare
b2749 ygbE +0.5 3.5 conserved inner membrane protein (NCBI) compare
b0965 b0965 +0.5 2.6 orf, hypothetical protein (VIMSS) compare
b2469 narQ +0.5 3.6 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b2236 yfaE +0.5 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1582 ynfA +0.5 2.6 hypothetical protein (NCBI) compare
b3977 tyrU +0.5 0.8 tRNA-Tyr (NCBI) compare
b4642 yoeD +0.5 2.0 no description compare
b4402 yjjY +0.5 1.1 hypothetical protein (NCBI) compare
b0247 ykfG +0.5 1.4 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1831 proQ +0.5 1.8 putative solute/DNA competence effector (NCBI) compare
b1355 b1355 +0.5 2.5 orf, hypothetical protein (VIMSS) compare
b2071 yegJ +0.5 1.1 hypothetical protein (NCBI) compare
b3194 yrbE +0.5 4.1 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1630 rsxD +0.5 2.4 electron transport complex protein RnfD (NCBI) compare
b0685 ybfE +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b1954 dsrA +0.5 2.4 regulatory antisense RNA (NCBI) compare
b3191 yrbB +0.5 4.1 orf, hypothetical protein (VIMSS) compare
b0759 galE +0.5 3.1 UDP-galactose-4-epimerase (VIMSS) compare
b1159 mcrA +0.5 0.9 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b1279 yciS +0.5 1.1 conserved inner membrane protein (NCBI) compare
b1627 rsxA +0.5 2.6 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1253 yciA +0.5 1.7 predicted hydrolase (NCBI) compare
b1696 ydiP +0.5 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1492 gadC +0.5 3.2 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b4041 plsB +0.5 1.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1160 elbA +0.5 0.7 hypothetical protein (NCBI) compare
b1165 ymgA +0.5 0.5 hypothetical protein (NCBI) compare
b3523 yhjE +0.5 3.9 predicted transporter (NCBI) compare
b3193 yrbD +0.4 3.4 predicted ABC-type organic solvent transporter (NCBI) compare
b1600 mdtJ +0.4 1.1 multidrug efflux system transporter (NCBI) compare
b2684 mprA +0.4 2.4 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2642 yfjW +0.4 0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3495 uspA +0.4 3.8 universal stress global response regulator (NCBI) compare
b3195 yrbF +0.4 3.7 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3929 rraA +0.4 3.2 ribonuclease activity regulator protein RraA (NCBI) compare
b3206 npr +0.4 2.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1841 yobA +0.4 1.0 hypothetical protein (NCBI) compare
b1482 osmC +0.4 1.4 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b1334 fnr +0.4 1.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1607 ydgC +0.4 1.2 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1461 ydcE +0.4 1.2 4-oxalocrotonate tautomerase (NCBI) compare
b2003 yeeT +0.4 1.7 CP4-44 prophage; predicted protein (NCBI) compare
b0782 moaB +0.4 1.0 molybdopterin biosynthesis protein B (NCBI) compare
b2450 yffS +0.4 1.4 CPZ-55 prophage; predicted protein (RefSeq) compare
b1608 rstA +0.4 2.2 DNA-binding response regulator in two-component regulatory system with RstB (RefSeq) compare
b1949 fliQ +0.4 1.1 flagellar biosynthesis protein Q (NCBI) compare
b2846 yqeH +0.4 0.4 orf, hypothetical protein (VIMSS) compare
b3397 nudE +0.4 2.9 ADP-ribose diphosphatase (NCBI) compare
b1919 yedO +0.4 1.9 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b2303 folX +0.4 3.0 D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI) compare
b2272 yfbM +0.4 1.7 hypothetical protein (NCBI) compare
b0364 yaiS +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1726 yniB +0.4 1.1 predicted inner membrane protein (NCBI) compare
b3688 yidQ +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b1530 marR +0.4 1.3 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3095 yqjA +0.4 2.6 conserved inner membrane protein (NCBI) compare
b0131 panD +0.4 2.2 aspartate 1-decarboxylase precursor (NCBI) compare
b2012 yeeD +0.4 1.7 hypothetical protein (NCBI) compare
b2078 baeS +0.4 2.7 sensory histidine kinase in two-component regulatory system with BaeR (NCBI) compare
b1709 btuD +0.4 0.6 vitamin B12-transporter ATPase (NCBI) compare
b0178 hlpA +0.4 2.2 periplasmic chaperone (NCBI) compare
b0661 miaB +0.3 2.8 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1777 b1777 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b2668 ygaP +0.3 2.6 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1480 sra +0.3 0.9 30S ribosomal subunit protein S22 (NCBI) compare
b1821 b1821 +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b0340 cynS +0.3 1.8 cyanate hydratase (NCBI) compare
b2291 yfbR +0.3 1.6 hypothetical protein (NCBI) compare
b3423 glpR +0.3 2.7 DNA-binding transcriptional repressor (NCBI) compare
b4509 ylcG +0.3 1.4 DLP12 prophage; predicted protein (NCBI) compare
b2502 ppx +0.3 2.7 exopolyphosphatase (NCBI) compare
b4519 icdC +0.3 2.1 no description compare
b3890 yiiF +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2204 napH +0.3 2.2 quinol dehydrogenase membrane component (NCBI) compare
b3512 GadE +0.3 1.8 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b3427 yzgL +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b4606 ypfM +0.3 1.8 hypothetical protein (RefSeq) compare
b1150 ymfR +0.3 0.6 e14 prophage; predicted protein (NCBI) compare
b3466 yhhL +0.3 2.1 conserved inner membrane protein (NCBI) compare
b1306 pspC +0.3 1.0 DNA-binding transcriptional activator (NCBI) compare
b2229 b2229 +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b1558 cspF +0.3 1.2 Qin prophage; cold shock protein (NCBI) compare
b1409 ynbB +0.3 0.6 predicted CDP-diglyceride synthase (NCBI) compare
b1970 yedX +0.3 1.6 hypothetical protein (NCBI) compare
b1048 mdoG +0.3 2.2 glucan biosynthesis protein, periplasmic (NCBI) compare
b4590 ybfK +0.3 0.4 hypothetical protein (RefSeq) compare
b4462 ygaQ +0.3 0.9 hypothetical protein (RefSeq) compare
b2212 alkB +0.3 1.7 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b0730 mngR +0.3 0.7 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1848 yebG +0.3 0.9 conserved protein regulated by LexA (NCBI) compare
b2948 yqgE +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b4559 yjdO +0.3 1.6 hypothetical protein (NCBI) compare
b3101 yqjF +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1104 ycfL +0.3 0.8 hypothetical protein (NCBI) compare
b0841 ybjG +0.3 2.1 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b4453 ldrD +0.3 1.4 toxic polypeptide, small (NCBI) compare
b3973 birA +0.3 1.1 biotin--protein ligase (NCBI) compare
b2903 gcvP +0.3 2.9 glycine dehydrogenase (NCBI) compare
b2444 yffM +0.3 1.3 CPZ-55 prophage; predicted protein (NCBI) compare
b3043 ygiL +0.3 1.8 predicted fimbrial-like adhesin protein (NCBI) compare
b0236 prfH +0.3 1.7 peptide chain release factor 2 (NCBI) compare
b1362 rzpR +0.3 1.2 putative Rac prophage endopeptidase (VIMSS) compare
b4173 hflX +0.3 2.0 predicted GTPase (NCBI) compare
b0357 yaiN +0.3 0.7 putative alpha helix chain (VIMSS) compare
b0122 yacC +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b0778 bioD +0.3 1.5 dethiobiotin synthetase (NCBI) compare
b1606 folM +0.3 2.1 short chain dehydrogenase (NCBI) compare
b2667 ygaV +0.3 2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b4640 yoeG +0.3 1.1 no description compare
b4596 yciZ +0.3 0.7 hypothetical protein (RefSeq) compare
b2625 yfjI +0.3 0.8 CP4-57 prophage; predicted protein (NCBI) compare
b2826 ppdA +0.3 2.5 hypothetical protein (NCBI) compare
b3602 yibL +0.3 2.3 hypothetical protein (NCBI) compare
b4391 yjjK +0.3 2.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0603 ybdO +0.3 0.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3500 gor +0.3 0.6 glutathione reductase (NCBI) compare
b0389 yaiA +0.3 1.2 hypothetical protein (NCBI) compare
b1250 kch +0.3 1.9 voltage-gated potassium channel (NCBI) compare
b2314 dedD +0.3 1.3 putative lipoprotein (VIMSS) compare
b3627 rfaI +0.3 2.3 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3180 yhbY +0.3 1.5 predicted RNA-binding protein (NCBI) compare
b2343 b2343 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1814 sdaA +0.3 1.2 L-serine deaminase I (NCBI) compare
b1890 motA +0.3 1.9 flagellar motor protein MotA (NCBI) compare
b4295 yjhU +0.3 2.4 orf, hypothetical protein (VIMSS) compare
b0535 fimZ +0.3 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2681 ygaY +0.3 2.5 predicted transporter (pseudogene) (RefSeq) compare
b4227 ytfQ +0.3 1.5 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b3888 yiiD +0.3 2.4 predicted acetyltransferase (NCBI) compare
b0958 sulA +0.3 1.5 SOS cell division inhibitor (NCBI) compare
b2308 hisQ +0.3 2.3 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b1632 rsxE +0.3 2.0 NADH-ubiquinone oxidoreductase (NCBI) compare
b4503 yafF +0.3 0.5 no description compare
b1121 ycfZ +0.3 0.9 predicted inner membrane protein (NCBI) compare
b1550 b1550 +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b0018 mokC +0.3 0.7 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4151 frdD +0.3 1.5 fumarate reductase subunit D (NCBI) compare
b2483 hyfC +0.3 1.8 hydrogenase 4 membrane subunit (VIMSS) compare
b0746 valZ +0.3 1.2 tRNA-Val (NCBI) compare
b1650 nemA +0.3 1.5 N-ethylmaleimide reductase, FMN-linked (NCBI) compare
b2006 yeeW +0.3 1.6 CP4-44 prophage; predicted protein (RefSeq) compare
b1108 ycfP +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2083 yegZ +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b2156 lysP +0.3 2.2 lysine transporter (NCBI) compare
b2421 cysM +0.3 1.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1920 fliY +0.3 0.7 cystine transporter subunit (NCBI) compare
b0375 yaiV +0.3 1.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3148 yraN +0.3 1.1 hypothetical protein (NCBI) compare
b0190 yaeQ +0.3 1.8 hypothetical protein (NCBI) compare
b4190 yjfP +0.3 2.0 predicted hydrolase (NCBI) compare
b0546 ybcM +0.3 1.7 DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1007 b1007 +0.3 0.8 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms