Experiment set53IT083 for Escherichia coli BW25113

Compare to:

diversity test of Keio_ML9a_ucsf

200 most detrimental genes:

  gene name fitness t score description  
b0912 ihfB +5.9 6.3 integration host factor subunit beta (NCBI) compare
b2215 ompC +4.9 13.7 outer membrane porin protein C (NCBI) compare
b0033 carB +4.0 19.6 carbamoyl-phosphate synthase large subunit (NCBI) compare
b4245 pyrB +3.9 19.3 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1874 cutC +3.8 6.2 copper homeostasis protein (NCBI) compare
b3405 ompR +3.3 4.8 osmolarity response regulator (NCBI) compare
b0032 carA +3.1 6.3 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0437 clpP +3.0 10.9 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3839 tatC +2.9 11.2 TatABCE protein translocation system subunit (NCBI) compare
b0740 tolB +2.8 4.9 translocation protein TolB precursor (NCBI) compare
b3396 mrcA +2.7 23.4 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b1712 ihfA +2.6 2.4 integration host factor subunit alpha (NCBI) compare
b3609 secB +2.5 8.9 export protein SecB (NCBI) compare
b4202 rpsR +2.5 3.4 30S ribosomal protein S18 (NCBI) compare
b0945 pyrD +2.5 14.8 dihydroorotate dehydrogenase (NCBI) compare
b4481 rffT +2.4 13.3 4-alpha-L-fucosyltransferase (NCBI) compare
b3082 ygjM +2.4 5.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1281 pyrF +2.4 6.7 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0053 surA +2.4 9.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2017 yefM +2.4 2.2 orf, hypothetical protein (VIMSS) compare
b0438 clpX +2.4 11.5 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3792 wzxE +2.4 11.8 O-antigen translocase (NCBI) compare
b1062 pyrC +2.4 14.0 dihydroorotase (NCBI) compare
b3035 tolC +2.3 5.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3791 rffA +2.3 13.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0737 tolQ +2.3 2.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0741 pal +2.3 5.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3642 pyrE +2.2 6.6 orotate phosphoribosyltransferase (NCBI) compare
b3790 rffC +2.2 10.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b3387 dam +2.2 16.2 DNA adenine methylase (NCBI) compare
b0738 tolR +2.2 2.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4246 pyrL +2.2 2.7 pyrBI operon leader peptide (NCBI) compare
b3169 nusA +2.1 4.9 transcription elongation factor NusA (NCBI) compare
b0739 tolA +2.1 4.0 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3404 envZ +2.1 8.4 osmolarity sensor protein (NCBI) compare
b1861 ruvA +2.1 2.1 Holliday junction DNA helicase motor protein (NCBI) compare
b3147 yraM +2.0 15.8 hypothetical protein (NCBI) compare
b2912 ygfA +2.0 7.1 putative ligase (VIMSS) compare
b2516 yfgA +2.0 9.7 hypothetical protein (NCBI) compare
b1192 ldcA +1.9 7.1 L,D-carboxypeptidase A (NCBI) compare
b3426 glpD +1.8 6.4 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b1236 galU +1.8 6.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0222 lpcA +1.8 4.4 phosphoheptose isomerase (NCBI) compare
b0386 proC +1.8 6.8 pyrroline-5-carboxylate reductase (NCBI) compare
b3842 rfaH +1.8 9.3 transcriptional activator RfaH (NCBI) compare
b1830 prc +1.7 8.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1863 ruvC +1.7 2.4 Holliday junction resolvase (NCBI) compare
b3052 rfaE +1.7 7.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3026 qseC +1.6 4.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0049 apaH +1.5 3.8 diadenosinetetraphosphatase (NCBI) compare
b3860 dsbA +1.5 9.9 periplasmic protein disulfide isomerase I (NCBI) compare
b3787 rffD +1.5 11.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3621 rfaC +1.5 6.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3785 wzzE +1.5 11.6 putative transport protein (VIMSS) compare
b2830 nudH +1.5 1.2 dinucleoside polyphosphate hydrolase (NCBI) compare
b4242 mgtA +1.4 11.4 magnesium transporter (NCBI) compare
b1235 rssB +1.4 8.4 response regulator of RpoS (NCBI) compare
b3786 rffE +1.4 11.6 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0683 fur +1.4 3.0 ferric uptake regulator (NCBI) compare
b1292 sapC +1.4 3.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0149 mrcB +1.4 11.7 penicillin-binding protein 1b (NCBI) compare
b3784 rfe +1.4 11.6 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3619 rfaD +1.4 4.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3631 rfaG +1.3 11.3 glucosyltransferase I (NCBI) compare
b0232 yafN +1.3 1.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2175 spr +1.3 6.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3620 rfaF +1.3 5.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b4702 mgtL +1.3 2.8 regulatory leader peptide for mgtA (NCBI) compare
b1084 rne +1.3 4.5 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2683 ygaH +1.3 4.4 predicted inner membrane protein (NCBI) compare
b3163 nlpI +1.3 6.3 hypothetical protein (NCBI) compare
b3794 rffM +1.2 8.2 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1286 rnb +1.2 3.8 exoribonuclease II (NCBI) compare
b1291 sapD +1.2 2.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1865 nudB +1.2 2.8 dATP pyrophosphohydrolase (NCBI) compare
b4172 hfq +1.2 1.4 RNA-binding protein Hfq (NCBI) compare
b1726 yniB +1.2 4.1 predicted inner membrane protein (NCBI) compare
b4006 purH +1.2 9.2 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b3390 aroK +1.1 2.4 shikimate kinase I (NCBI) compare
b1185 dsbB +1.1 4.5 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4218 ytfL +1.1 9.0 predicted inner membrane protein (NCBI) compare
b3630 rfaP +1.1 8.0 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1855 lpxM +1.1 4.9 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b2312 purF +1.1 7.0 amidophosphoribosyltransferase (NCBI) compare
b0523 purE +1.1 5.2 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4414 tff +1.1 2.4 unknown RNA (NCBI) compare
b1175 minD +1.1 5.9 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1111 ycfQ +1.1 3.7 orf, hypothetical protein (VIMSS) compare
b2476 purC +1.1 4.4 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1290 sapF +1.1 2.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1804 rnd +1.0 6.1 ribonuclease D (NCBI) compare
b1831 proQ +1.0 3.8 putative solute/DNA competence effector (NCBI) compare
b3962 udhA +1.0 6.5 putative oxidoreductase (VIMSS) compare
b2499 purM +1.0 5.1 phosphoribosylaminoimidazole synthetase (NCBI) compare
b1176 minC +1.0 4.1 septum formation inhibitor (NCBI) compare
b3706 trmE +1.0 4.2 tRNA modification GTPase (NCBI) compare
b1677 lpp +1.0 3.0 murein lipoprotein (NCBI) compare
b0161 degP +1.0 4.5 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b0630 lipB +1.0 2.0 protein of lipoate biosynthesis (VIMSS) compare
b0051 ksgA +0.9 5.3 dimethyladenosine transferase (NCBI) compare
b2911 ssrS +0.9 1.6 6S regulatory RNA (NCBI) compare
b2557 purL +0.9 7.2 phosphoribosylformylglycinamidine synthase (NCBI) compare
b3236 mdh +0.9 6.6 malate dehydrogenase (NCBI) compare
b3389 aroB +0.9 7.2 3-dehydroquinate synthase (NCBI) compare
b3281 aroE +0.8 7.3 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0729 sucD +0.8 4.4 succinyl-CoA synthetase subunit alpha (NCBI) compare
b4239 treC +0.8 1.9 trehalose-6-P hydrolase (NCBI) compare
b0688 pgm +0.8 3.1 phosphoglucomutase (NCBI) compare
b4005 purD +0.8 6.3 phosphoribosylamine--glycine ligase (NCBI) compare
b3021 ygiT +0.8 3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1095 fabF +0.8 5.2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3838 tatB +0.8 4.1 sec-independent translocase (NCBI) compare
b1936 intG +0.8 1.2 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3611 yibN +0.8 3.9 predicted rhodanese-related sulfurtransferase (NCBI) compare
b2414 cysK +0.8 6.0 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b1613 manA +0.8 2.2 mannose-6-phosphate isomerase (VIMSS) compare
b2951 yggS +0.8 4.2 predicted enzyme (NCBI) compare
b4179 vacB +0.8 4.3 putative enzyme (VIMSS) compare
b0522 purK +0.8 5.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b3613 yibP +0.8 2.0 putative membrane protein (VIMSS) compare
b0889 lrp +0.8 3.6 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1693 aroD +0.8 5.1 3-dehydroquinate dehydratase (NCBI) compare
b0200 gmhB +0.8 1.2 hypothetical protein (NCBI) compare
b3912 cpxR +0.8 5.8 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b1850 eda +0.7 2.8 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1103 ycfF +0.7 2.3 orf, hypothetical protein (VIMSS) compare
b2576 srmB +0.7 3.0 ATP-dependent RNA helicase (NCBI) compare
b4128 yjdK +0.7 0.6 hypothetical protein (NCBI) compare
b3644 yicC +0.7 3.8 hypothetical protein (NCBI) compare
b2820 recB +0.7 0.5 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1105 ycfM +0.7 3.4 predicted outer membrane lipoprotein (NCBI) compare
b0439 lon +0.7 3.2 DNA-binding ATP-dependent protease La (NCBI) compare
b2313 cvpA +0.7 4.3 membrane protein required for colicin V production (NCBI) compare
b2518 ndk +0.7 2.4 nucleoside diphosphate kinase (NCBI) compare
b1600 mdtJ +0.7 1.8 multidrug efflux system transporter (NCBI) compare
b2363 yfdT +0.7 2.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1104 ycfL +0.7 1.7 hypothetical protein (NCBI) compare
b3290 trkA +0.7 1.3 potassium transporter peripheral membrane component (NCBI) compare
b0632 dacA +0.7 4.9 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3778 rep +0.7 4.6 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3493 pitA +0.7 4.7 phosphate transporter, low-affinity (NCBI) compare
b2531 iscR +0.7 2.1 DNA-binding transcriptional repressor (NCBI) compare
b3204 ptsN +0.7 3.7 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1293 sapB +0.7 1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1860 ruvB +0.7 0.5 Holliday junction DNA helicase B (NCBI) compare
b2569 lepA +0.7 3.0 GTP-binding protein LepA (NCBI) compare
b3055 htrG +0.6 5.0 predicted signal transduction protein (SH3 domain) (NCBI) compare
b2424 cysU +0.6 3.1 sulfate/thiosulfate transporter subunit (NCBI) compare
b3443 yrhA +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b2688 gshA +0.6 4.4 glutamate--cysteine ligase (NCBI) compare
b1109 ndh +0.6 3.2 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1777 b1777 +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b4163 glyV +0.6 2.0 tRNA-Gly (NCBI) compare
b2501 ppk +0.6 4.7 polyphosphate kinase (NCBI) compare
b3940 metL +0.6 4.5 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b3198 kdsC +0.6 2.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b4042 dgkA +0.6 0.8 diacylglycerol kinase (NCBI) compare
b2846 yqeH +0.6 0.6 orf, hypothetical protein (VIMSS) compare
b4171 miaA +0.6 2.4 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0114 aceE +0.6 0.7 pyruvate dehydrogenase subunit E1 (NCBI) compare
b2903 gcvP +0.6 5.1 glycine dehydrogenase (NCBI) compare
b2905 gcvT +0.6 4.1 glycine cleavage system aminomethyltransferase T (NCBI) compare
b0890 ftsK +0.6 2.1 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2413 cysZ +0.6 3.8 putative sulfate transport protein CysZ (NCBI) compare
b3741 gidA +0.6 2.4 glucose-inhibited division protein A (NCBI) compare
b2318 truA +0.6 2.1 tRNA pseudouridine synthase A (NCBI) compare
b0920 ycbC +0.6 3.3 conserved inner membrane protein (NCBI) compare
b3261 fis +0.6 1.5 DNA-binding protein Fis (NCBI) compare
b3652 recG +0.6 4.5 ATP-dependent DNA helicase (NCBI) compare
b4402 yjjY +0.6 1.3 hypothetical protein (NCBI) compare
b0143 pcnB +0.6 4.0 poly(A) polymerase I (VIMSS) compare
b1548 nohA +0.6 2.1 Qin prophage; predicted packaging protein (RefSeq) compare
b2934 cmtB +0.6 0.7 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b0720 gltA +0.6 4.1 citrate synthase (NCBI) compare
b2512 yfgL +0.6 2.9 protein assembly complex, lipoprotein component (NCBI) compare
b0728 sucC +0.6 3.3 succinyl-CoA synthetase subunit beta (NCBI) compare
b2708 gutQ +0.6 3.2 orf, hypothetical protein (VIMSS) compare
b1841 yobA +0.5 2.0 hypothetical protein (NCBI) compare
b2808 gcvA +0.5 2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2083 yegZ +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1553 ydfP +0.5 1.8 Qin prophage; conserved protein (NCBI) compare
b3806 cyaA +0.5 2.9 adenylate cyclase (NCBI) compare
b0243 proA +0.5 4.3 gamma-glutamyl phosphate reductase (NCBI) compare
b3252 yhdA +0.5 4.6 conserved inner membrane protein (NCBI) compare
b3500 gor +0.5 1.2 glutathione reductase (NCBI) compare
b3398 yrfF +0.5 2.4 predicted inner membrane protein (NCBI) compare
b4175 hflC +0.5 4.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3863 polA +0.5 0.5 DNA polymerase I (NCBI) compare
b4603 rseX +0.5 0.7 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1536 ydeI +0.5 0.9 hypothetical protein (NCBI) compare
b4174 hflK +0.5 4.4 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1304 pspA +0.5 2.5 regulatory protein for phage-shock-protein operon (NCBI) compare
b2012 yeeD +0.5 1.6 hypothetical protein (NCBI) compare
b2319 usg +0.5 2.7 hypothetical protein (NCBI) compare
b1583 ynfB +0.5 2.5 hypothetical protein (NCBI) compare
b2904 gcvH +0.5 1.6 glycine cleavage system protein H (NCBI) compare
b3133 agaV +0.5 1.4 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b3164 pnp +0.5 2.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4201 priB +0.5 1.7 primosomal replication protein N (NCBI) compare
b4642 yoeD +0.5 2.0 no description compare


Specific Phenotypes

For 3 genes in this experiment