Experiment set53IT075 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 1.98 mM 24 hr

200 most important genes:

  gene name fitness t score description  
b2512 yfgL -4.2 -8.0 protein assembly complex, lipoprotein component (NCBI) compare
b2808 gcvA -4.2 -4.1 DNA-binding transcriptional dual regulator (NCBI) compare
b0463 acrA -3.6 -14.2 multidrug efflux system (NCBI) compare
b3183 obgE -3.3 -2.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4242 mgtA -3.2 -3.1 magnesium transporter (NCBI) compare
b3860 dsbA -3.1 -9.0 periplasmic protein disulfide isomerase I (NCBI) compare
b1185 dsbB -3.0 -2.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1855 lpxM -2.9 -6.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4702 mgtL -2.8 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b4171 miaA -2.7 -4.7 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2188 yejM -2.6 -12.2 predicted hydrolase, inner membrane (NCBI) compare
b0683 fur -2.6 -1.7 ferric uptake regulator (NCBI) compare
b3844 fre -2.5 -10.0 NAD(P)H-flavin reductase (NCBI) compare
b1865 nudB -2.5 -2.3 dATP pyrophosphohydrolase (NCBI) compare
b0462 acrB -2.3 -19.0 multidrug efflux system protein (NCBI) compare
b0145 dksA -2.2 -4.2 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3052 rfaE -2.2 -1.5 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0632 dacA -2.2 -9.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0439 lon -2.1 -4.0 DNA-binding ATP-dependent protease La (NCBI) compare
b3979 thrT -2.0 -0.9 tRNA-Thr (NCBI) compare
b0438 clpX -1.9 -2.8 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0564 appY -1.8 -1.0 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1438 ydcQ -1.8 -3.4 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0400 phoR -1.8 -10.4 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b2617 smpA -1.8 -5.4 small membrane protein A (VIMSS) compare
b2501 ppk -1.8 -10.1 polyphosphate kinase (NCBI) compare
b4414 tff -1.8 -2.9 unknown RNA (NCBI) compare
b0080 fruR -1.7 -7.4 DNA-binding transcriptional dual regulator (NCBI) compare
b4005 purD -1.7 -10.3 phosphoribosylamine--glycine ligase (NCBI) compare
b2318 truA -1.7 -3.3 tRNA pseudouridine synthase A (NCBI) compare
b0222 lpcA -1.7 -1.9 phosphoheptose isomerase (NCBI) compare
b2312 purF -1.6 -6.0 amidophosphoribosyltransferase (NCBI) compare
b2330 yfcB -1.6 -2.7 putative adenine-specific methylase (VIMSS) compare
b4375 prfC -1.6 -6.5 peptide chain release factor 3 (NCBI) compare
b3493 pitA -1.5 -7.7 phosphate transporter, low-affinity (NCBI) compare
b0688 pgm -1.5 -3.1 phosphoglucomutase (NCBI) compare
b4006 purH -1.5 -9.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b2313 cvpA -1.5 -3.8 membrane protein required for colicin V production (NCBI) compare
b0437 clpP -1.5 -1.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4201 priB -1.4 -3.3 primosomal replication protein N (NCBI) compare
b3792 wzxE -1.4 -2.8 O-antigen translocase (NCBI) compare
b4179 vacB -1.4 -4.3 putative enzyme (VIMSS) compare
b3621 rfaC -1.4 -2.2 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0522 purK -1.4 -7.4 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b0049 apaH -1.3 -1.2 diadenosinetetraphosphatase (NCBI) compare
b1235 rssB -1.3 -4.4 response regulator of RpoS (NCBI) compare
b0200 gmhB -1.3 -0.9 hypothetical protein (NCBI) compare
b4146 yjeK -1.3 -2.1 predicted lysine aminomutase (NCBI) compare
b3870 glnA -1.3 -3.4 glutamine synthetase (NCBI) compare
b2071 yegJ -1.3 -2.5 hypothetical protein (NCBI) compare
b4654 yjdQ -1.3 -4.7 no description compare
b0741 pal -1.3 -0.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0053 surA -1.3 -1.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0226 dinJ -1.3 -4.0 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1677 lpp -1.2 -1.9 murein lipoprotein (NCBI) compare
b3916 pfkA -1.2 -3.3 6-phosphofructokinase (NCBI) compare
b1192 ldcA -1.2 -2.2 L,D-carboxypeptidase A (NCBI) compare
b1332 ynaJ -1.2 -1.0 predicted inner membrane protein (NCBI) compare
b4203 rplI -1.2 -4.6 50S ribosomal protein L9 (NCBI) compare
b3162 deaD -1.2 -4.9 inducible ATP-independent RNA helicase (VIMSS) compare
b1304 pspA -1.2 -3.1 regulatory protein for phage-shock-protein operon (NCBI) compare
b3261 fis -1.2 -1.8 DNA-binding protein Fis (NCBI) compare
b3620 rfaF -1.2 -3.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0142 folK -1.2 -0.8 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2285 nuoE -1.1 -2.5 NADH dehydrogenase subunit E (NCBI) compare
b2912 ygfA -1.1 -2.2 putative ligase (VIMSS) compare
b3838 tatB -1.1 -4.5 sec-independent translocase (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b0051 ksgA -1.1 -4.5 dimethyladenosine transferase (NCBI) compare
b2287 nuoB -1.1 -3.0 NADH dehydrogenase subunit B (NCBI) compare
b0726 sucA -1.1 -4.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b0767 ybhE -1.1 -3.6 6-phosphogluconolactonase (NCBI) compare
b4701 sokX -1.1 -1.3 sok-related sRNA, function unknown (RefSeq) compare
b1676 pykF -1.1 -4.2 pyruvate kinase (NCBI) compare
b3630 rfaP -1.1 -5.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4481 rffT -1.1 -3.5 4-alpha-L-fucosyltransferase (NCBI) compare
b0386 proC -1.1 -2.3 pyrroline-5-carboxylate reductase (NCBI) compare
b1871 yecP -1.1 -4.7 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b3199 yrbK -1.1 -2.0 hypothetical protein (NCBI) compare
b1850 eda -1.0 -1.6 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2494 yfgC -1.0 -7.5 predicted peptidase (NCBI) compare
b3164 pnp -1.0 -1.9 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1837 yebW -1.0 -1.8 orf, hypothetical protein (VIMSS) compare
b3204 ptsN -1.0 -4.1 sugar-specific enzyme IIA component of PTS (NCBI) compare
b4180 rlmB -1.0 -6.9 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b2784 relA -1.0 -7.3 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b3545 proK -1.0 -2.9 tRNA-Pro (NCBI) compare
b3849 trkH -1.0 -7.3 potassium transporter (NCBI) compare
b1109 ndh -1.0 -2.8 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b4480 hdfR -1.0 -5.4 transcriptional regulator HdfR (NCBI) compare
b1231 tyrT -0.9 -1.1 tRNA-Tyr (NCBI) compare
b2499 purM -0.9 -3.1 phosphoribosylaminoimidazole synthetase (NCBI) compare
b4000 hupA -0.9 -5.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b4174 hflK -0.9 -6.7 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0928 aspC -0.9 -4.6 aspartate aminotransferase, PLP-dependent (NCBI) compare
b0149 mrcB -0.9 -5.9 penicillin-binding protein 1b (NCBI) compare
b0143 pcnB -0.9 -4.2 poly(A) polymerase I (VIMSS) compare
b2905 gcvT -0.8 -4.5 glycine cleavage system aminomethyltransferase T (NCBI) compare
b0729 sucD -0.8 -3.1 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2620 smpB -0.8 -0.9 SsrA-binding protein (NCBI) compare
b3791 rffA -0.8 -3.1 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4025 pgi -0.8 -5.6 glucose-6-phosphate isomerase (NCBI) compare
b3706 trmE -0.8 -2.5 tRNA modification GTPase (NCBI) compare
b2278 nuoL -0.8 -5.2 NADH dehydrogenase subunit L (NCBI) compare
b2576 srmB -0.8 -2.7 ATP-dependent RNA helicase (NCBI) compare
b1291 sapD -0.8 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3781 trxA -0.8 -3.0 thioredoxin 1 (VIMSS) compare
b2082 ogrK -0.8 -2.5 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b1550 b1550 -0.8 -1.3 orf, hypothetical protein (VIMSS) compare
b0032 carA -0.8 -2.7 carbamoyl-phosphate synthase small subunit (NCBI) compare
b1982 amn -0.8 -6.3 AMP nucleosidase (NCBI) compare
b3652 recG -0.8 -5.4 ATP-dependent DNA helicase (NCBI) compare
b2279 nuoK -0.8 -1.1 NADH dehydrogenase subunit K (NCBI) compare
b2329 aroC -0.8 -3.6 chorismate synthase (NCBI) compare
b1160 elbA -0.8 -0.7 hypothetical protein (NCBI) compare
b2493 yfgO -0.8 -5.6 predicted inner membrane protein (NCBI) compare
b4175 hflC -0.8 -5.0 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1429 tehA -0.8 -1.0 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b3631 rfaG -0.8 -5.3 glucosyltransferase I (NCBI) compare
b2277 nuoM -0.8 -3.8 NADH dehydrogenase subunit M (NCBI) compare
b3728 pstS -0.8 -2.6 phosphate transporter subunit (NCBI) compare
b3494 yhiO -0.8 -5.0 universal stress protein UspB (NCBI) compare
b0908 aroA -0.8 -4.3 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3761 trpT -0.8 -1.8 tRNA-Trp (NCBI) compare
b0059 hepA -0.7 -6.8 ATP-dependent helicase HepA (NCBI) compare
b1613 manA -0.7 -1.1 mannose-6-phosphate isomerase (VIMSS) compare
b3082 ygjM -0.7 -0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3867 hemN -0.7 -5.6 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b2284 nuoF -0.7 -2.4 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2846 yqeH -0.7 -0.4 orf, hypothetical protein (VIMSS) compare
b3977 tyrU -0.7 -0.8 tRNA-Tyr (NCBI) compare
b1709 btuD -0.7 -0.8 vitamin B12-transporter ATPase (NCBI) compare
b3741 gidA -0.7 -1.7 glucose-inhibited division protein A (NCBI) compare
b1084 rne -0.7 -2.5 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b1686 ydiI -0.7 -1.6 hypothetical protein (NCBI) compare
b2288 nuoA -0.7 -2.4 NADH dehydrogenase I chain A (VIMSS) compare
b2276 nuoN -0.7 -3.0 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3869 glnL -0.7 -4.4 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b3398 yrfF -0.7 -2.1 predicted inner membrane protein (NCBI) compare
b3790 rffC -0.7 -2.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b2017 yefM -0.7 -0.7 orf, hypothetical protein (VIMSS) compare
b1255 yciC -0.7 -3.6 hypothetical protein (NCBI) compare
b0481 ybaK -0.7 -2.7 hypothetical protein (NCBI) compare
b1290 sapF -0.7 -0.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0926 ycbK -0.7 -3.9 hypothetical protein (NCBI) compare
b2557 purL -0.7 -4.8 phosphoribosylformylglycinamidine synthase (NCBI) compare
b4218 ytfL -0.6 -4.8 predicted inner membrane protein (NCBI) compare
b2414 cysK -0.6 -4.5 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b0782 moaB -0.6 -1.3 molybdopterin biosynthesis protein B (NCBI) compare
b3414 gntY -0.6 -3.0 predicted gluconate transport associated protein (NCBI) compare
b2683 ygaH -0.6 -2.8 predicted inner membrane protein (NCBI) compare
b3289 rsmB -0.6 -5.2 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b3229 sspA -0.6 -2.4 stringent starvation protein A (NCBI) compare
b1107 nagZ -0.6 -2.4 beta-hexosaminidase (NCBI) compare
b0677 nagA -0.6 -2.8 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0912 ihfB -0.6 -0.4 integration host factor subunit beta (NCBI) compare
b2421 cysM -0.6 -2.1 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0019 nhaA -0.6 -4.4 pH-dependent sodium/proton antiporter (NCBI) compare
b4243 yjgF -0.6 -3.4 orf, hypothetical protein (VIMSS) compare
b0724 sdhB -0.6 -2.9 succinate dehydrogenase, FeS subunit (NCBI) compare
b0889 lrp -0.6 -1.4 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1368 ynaA -0.6 -1.9 Rac prophage; predicted tail protein (RefSeq) compare
b4647 mokA -0.6 -0.7 no description compare
b4245 pyrB -0.6 -1.7 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0888 trxB -0.6 -2.3 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b2280 nuoJ -0.6 -3.0 NADH dehydrogenase subunit J (NCBI) compare
b3825 pldB -0.6 -5.0 lysophospholipase L(2) (NCBI) compare
b2951 yggS -0.6 -2.0 predicted enzyme (NCBI) compare
b1161 ycgX -0.6 -0.6 hypothetical protein (NCBI) compare
b2569 lepA -0.6 -2.0 GTP-binding protein LepA (NCBI) compare
b3396 mrcA -0.6 -4.4 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b0523 purE -0.6 -1.9 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b2258 b2258 -0.6 -1.0 putative transport/receptor protein (VIMSS) compare
b3497 yhiQ -0.6 -3.9 predicted SAM-dependent methyltransferase (NCBI) compare
b0088 murD -0.6 -1.0 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3260 dusB -0.6 -3.1 tRNA-dihydrouridine synthase B (NCBI) compare
b3619 rfaD -0.6 -0.8 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3742 mioC -0.6 -3.2 flavodoxin (NCBI) compare
b0406 tgt -0.6 -3.8 queuine tRNA-ribosyltransferase (NCBI) compare
b1114 mfd -0.6 -4.1 transcription-repair coupling factor (NCBI) compare
b1063 yceB -0.6 -1.7 predicted lipoprotein (NCBI) compare
b1292 sapC -0.6 -0.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3863 polA -0.5 -0.3 DNA polymerase I (NCBI) compare
b0723 sdhA -0.5 -4.3 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3375 yhfR -0.5 -3.7 putative transcriptional regulator (VIMSS) compare
b0882 clpA -0.5 -3.9 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b0125 hpt -0.5 -3.1 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1346 ydaQ -0.5 -0.6 orf, hypothetical protein (VIMSS) compare
b3764 yifE -0.5 -3.2 hypothetical protein (NCBI) compare
b2903 gcvP -0.5 -4.6 glycine dehydrogenase (NCBI) compare
b1148 ymfM -0.5 -2.2 e14 prophage; predicted protein (NCBI) compare
b3021 ygiT -0.5 -1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4472 yhdP -0.5 -5.0 conserved membrane protein, predicted transporter (NCBI) compare
b3965 trmA -0.5 -4.2 tRNA (uracil-5-)-methyltransferase (NCBI) compare
b3388 damX -0.5 -4.6 hypothetical protein (NCBI) compare
b3806 cyaA -0.5 -2.7 adenylate cyclase (NCBI) compare
b1286 rnb -0.5 -1.0 exoribonuclease II (NCBI) compare
b1358 ydaT -0.5 -0.7 Rac prophage; predicted protein (NCBI) compare
b0840 deoR -0.5 -2.6 DNA-binding transcriptional repressor (NCBI) compare
b2283 nuoG -0.5 -3.2 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare


Specific Phenotypes

For 5 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms