Experiment set53IT075 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 1.98 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b1247 oppF +2.0 10.9 oligopeptide transporter subunit (NCBI) compare
b1243 oppA +1.8 10.5 oligopeptide transporter subunit (NCBI) compare
b1246 oppD +1.7 10.4 oligopeptide transporter ATP-binding component (NCBI) compare
b1245 oppC +1.7 13.2 oligopeptide transporter subunit (NCBI) compare
b1936 intG +1.6 2.3 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2469 narQ +1.6 4.5 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b2684 mprA +1.4 4.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1244 oppB +1.4 10.5 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b3911 cpxA +1.3 10.1 two-component sensor protein (NCBI) compare
b1765 ydjA +1.2 1.6 predicted oxidoreductase (NCBI) compare
b1095 fabF +1.1 4.2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4133 cadC +1.0 9.2 DNA-binding transcriptional activator (NCBI) compare
b4593 ymgI +0.9 1.4 hypothetical protein (RefSeq) compare
b1524 yneH +0.9 1.6 predicted glutaminase (NCBI) compare
b1600 mdtJ +0.8 2.0 multidrug efflux system transporter (NCBI) compare
b1823 cspC +0.8 3.1 stress protein, member of the CspA-family (NCBI) compare
b2189 proL +0.8 2.9 tRNA-Pro (NCBI) compare
b4313 fimE +0.8 6.7 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1630 rsxD +0.8 4.4 electron transport complex protein RnfD (NCBI) compare
b2659 b2659 +0.8 3.7 orf, hypothetical protein (VIMSS) compare
b3755 yieP +0.8 4.7 predicted transcriptional regulator (NCBI) compare
b3390 aroK +0.8 2.0 shikimate kinase I (NCBI) compare
b0464 acrR +0.8 6.4 DNA-binding transcriptional repressor (NCBI) compare
b2502 ppx +0.8 5.9 exopolyphosphatase (NCBI) compare
b1628 rsxB +0.7 3.6 electron transport complex protein RnfB (NCBI) compare
b3609 secB +0.7 2.5 export protein SecB (NCBI) compare
b2078 baeS +0.7 2.5 sensory histidine kinase in two-component regulatory system with BaeR (NCBI) compare
b4536 yobH +0.7 3.9 hypothetical protein (NCBI) compare
b1627 rsxA +0.7 3.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1629 rsxC +0.7 4.0 electron transport complex protein RnfC (NCBI) compare
b1575 dicB +0.7 1.6 Qin prophage; cell division inhibition protein (NCBI) compare
b1591 b1591 +0.7 1.5 putative oxidoreductase component (VIMSS) compare
b1954 dsrA +0.7 3.1 regulatory antisense RNA (NCBI) compare
b4590 ybfK +0.7 0.9 hypothetical protein (RefSeq) compare
b1001 yccE +0.7 0.4 hypothetical protein (NCBI) compare
b4426 isrA +0.6 2.2 unknown RNA (NCBI) compare
b2217 rcsB +0.6 4.3 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3194 yrbE +0.6 5.4 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1362 rzpR +0.6 1.9 putative Rac prophage endopeptidase (VIMSS) compare
b1497 b1497 +0.6 0.3 putative enzyme (VIMSS) compare
b1576 ydfD +0.6 0.3 Qin prophage; predicted protein (NCBI) compare
b4679 yohP +0.6 2.9 expressed protein (RefSeq) compare
b1279 yciS +0.6 1.3 conserved inner membrane protein (NCBI) compare
b2642 yfjW +0.6 1.1 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1409 ynbB +0.6 1.0 predicted CDP-diglyceride synthase (NCBI) compare
b3779 gpp +0.6 5.6 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b3429 glgA +0.6 5.3 glycogen synthase (NCBI) compare
b3146 yraL +0.6 4.8 predicted methyltransferase (NCBI) compare
b4559 yjdO +0.6 3.0 hypothetical protein (NCBI) compare
b2450 yffS +0.6 1.5 CPZ-55 prophage; predicted protein (RefSeq) compare
b2911 ssrS +0.6 1.1 6S regulatory RNA (NCBI) compare
b2741 rpoS +0.6 4.5 RNA polymerase sigma factor (NCBI) compare
b3397 nudE +0.6 4.9 ADP-ribose diphosphatase (NCBI) compare
b1607 ydgC +0.6 1.4 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1545 pinQ +0.6 0.2 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1347 ydaC +0.6 0.3 Rac prophage; predicted protein (NCBI) compare
b4676 yoaK +0.6 0.6 expressed protein (RefSeq) compare
b2214 yojL +0.6 4.3 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b0065 yabI +0.6 3.6 conserved inner membrane protein (NCBI) compare
b2363 yfdT +0.5 1.9 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1134 ymfB +0.5 1.8 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1693 aroD +0.5 2.5 3-dehydroquinate dehydratase (NCBI) compare
b1363 trkG +0.5 0.4 Rac prophage; potassium transporter subunit (NCBI) compare
b2181 yejG +0.5 2.5 hypothetical protein (NCBI) compare
b2061 wzb +0.5 1.8 protein-tyrosine phosphatase (NCBI) compare
b1499 ydeO +0.5 0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1236 galU +0.5 1.4 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1357 ydaS +0.5 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1773 ydjI +0.5 1.5 predicted aldolase (NCBI) compare
b1257 yciE +0.5 0.2 hypothetical protein (NCBI) compare
b0661 miaB +0.5 4.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1334 fnr +0.5 2.1 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2698 recX +0.5 2.3 RecA regulator RecX (NCBI) compare
b1505 ydeT +0.5 0.9 hypothetical protein (RefSeq) compare
b1976 b1976 +0.5 2.5 orf, hypothetical protein (VIMSS) compare
b1863 ruvC +0.5 0.5 Holliday junction resolvase (NCBI) compare
b2960 yggH +0.5 3.7 tRNA(m7G46)-methyltransferase (NCBI) compare
b4246 pyrL +0.5 1.0 pyrBI operon leader peptide (NCBI) compare
b4570 lomR +0.5 1.7 no description compare
b3292 zntR +0.5 2.3 zinc-responsive transcriptional regulator (NCBI) compare
b1492 gadC +0.5 3.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1143 ymfI +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b3884 yihW +0.5 3.1 putative DEOR-type transcriptional regulator (VIMSS) compare
b1984 asnW +0.5 0.9 tRNA-Asn (NCBI) compare
b1632 rsxE +0.5 3.2 NADH-ubiquinone oxidoreductase (NCBI) compare
b3071 yqjI +0.5 2.7 predicted transcriptional regulator (NCBI) compare
b0685 ybfE +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1167 ymgC +0.5 0.2 hypothetical protein (NCBI) compare
b0929 ompF +0.5 3.1 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2177 yejA +0.5 3.5 orf, hypothetical protein (VIMSS) compare
b4695 ykgT +0.5 1.8 no description compare
b4592 yccB +0.5 1.2 hypothetical protein (RefSeq) compare
b3495 uspA +0.5 4.1 universal stress global response regulator (NCBI) compare
b2180 yejF +0.5 3.5 fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI) compare
b1824 yobF +0.4 1.4 hypothetical protein (NCBI) compare
b1400 paaY +0.4 1.0 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1540 ydfH +0.4 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1848 yebG +0.4 1.1 conserved protein regulated by LexA (NCBI) compare
b3753 rbsR +0.4 3.8 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b4545 ypdJ +0.4 1.0 no description compare
b2448 yffQ +0.4 2.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b2252 ais +0.4 2.5 hypothetical protein (NCBI) compare
b1104 ycfL +0.4 0.7 hypothetical protein (NCBI) compare
b2531 iscR +0.4 1.1 DNA-binding transcriptional repressor (NCBI) compare
b0843 ybjH +0.4 1.8 hypothetical protein (NCBI) compare
b1530 marR +0.4 1.2 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1325 ycjG +0.4 1.5 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b0303 ykgI +0.4 0.5 orf, hypothetical protein (VIMSS) compare
b0001 thrL +0.4 2.0 thr operon leader peptide (NCBI) compare
b1461 ydcE +0.4 1.7 4-oxalocrotonate tautomerase (NCBI) compare
b1360 ydaV +0.4 1.5 Rac prophage; predicted DNA replication protein (NCBI) compare
b2637 yfjT +0.4 1.4 CP4-57 prophage; predicted protein (NCBI) compare
b0802 ybiJ +0.4 1.3 hypothetical protein (NCBI) compare
b2945 endA +0.4 2.8 DNA-specific endonuclease I (NCBI) compare
b1604 ydgH +0.4 1.7 hypothetical protein (NCBI) compare
b1330 ynaI +0.4 1.9 conserved inner membrane protein (NCBI) compare
b1295 ymjA +0.4 1.6 hypothetical protein (NCBI) compare
b3676 yidH +0.4 2.6 conserved inner membrane protein (NCBI) compare
b3192 yrbC +0.4 3.3 predicted ABC-type organic solvent transporter (NCBI) compare
b0933 ssuB +0.4 2.6 alkanesulfonate transporter subunit (NCBI) compare
b1249 cls +0.4 2.7 cardiolipin synthetase (NCBI) compare
b1048 mdoG +0.4 2.6 glucan biosynthesis protein, periplasmic (NCBI) compare
b0965 b0965 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3191 yrbB +0.4 3.2 orf, hypothetical protein (VIMSS) compare
b2571 rseB +0.4 3.2 periplasmic negative regulator of sigmaE (NCBI) compare
b1250 kch +0.4 2.6 voltage-gated potassium channel (NCBI) compare
b1040 csgD +0.4 0.8 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b2006 yeeW +0.4 2.0 CP4-44 prophage; predicted protein (RefSeq) compare
b4558 yifL +0.4 2.2 predicted lipoprotein (NCBI) compare
b0982 yccY +0.4 2.2 putative phosphatase (VIMSS) compare
b0535 fimZ +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1790 yeaM +0.4 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b4345 mcrC +0.4 0.7 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1842 holE +0.4 1.2 DNA polymerase III, theta subunit (NCBI) compare
b2528 iscA +0.4 0.9 iron-sulfur cluster assembly protein (NCBI) compare
b1712 ihfA +0.4 0.2 integration host factor subunit alpha (NCBI) compare
b2690 yqaB +0.4 2.4 predicted hydrolase (NCBI) compare
b1818 manY +0.4 1.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b2229 b2229 +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b0955 ycbZ +0.4 2.7 predicted peptidase (NCBI) compare
b2346 vacJ +0.4 2.8 predicted lipoprotein (NCBI) compare
b1527 yneK +0.4 1.0 hypothetical protein (NCBI) compare
b2419 yfeK +0.4 1.3 hypothetical protein (NCBI) compare
b4699 fnrS +0.4 0.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3816 corA +0.4 2.8 magnesium/nickel/cobalt transporter (NCBI) compare
b4181 yjfI +0.4 1.5 hypothetical protein (NCBI) compare
b3206 npr +0.4 2.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1306 pspC +0.4 1.5 DNA-binding transcriptional activator (NCBI) compare
b0660 ybeZ +0.4 2.3 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b0104 guaC +0.4 2.8 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b1150 ymfR +0.4 0.6 e14 prophage; predicted protein (NCBI) compare
b4222 ytfP +0.4 1.9 hypothetical protein (NCBI) compare
b2179 yejE +0.4 2.3 predicted oligopeptide transporter subunit (NCBI) compare
b3014 yqhH +0.4 1.8 predicted outer membrane lipoprotein (NCBI) compare
b1895 yecG +0.4 2.2 universal stress protein (NCBI) compare
b1548 nohA +0.4 1.1 Qin prophage; predicted packaging protein (RefSeq) compare
b2058 wcaB +0.4 0.7 predicted acyl transferase (NCBI) compare
b4383 deoB +0.3 2.8 phosphopentomutase (NCBI) compare
b3054 ygiF +0.3 2.6 predicted adenylate cyclase (NCBI) compare
b1049 mdoH +0.3 2.7 glucosyltransferase MdoH (NCBI) compare
b3812 yigB +0.3 2.4 predicted hydrolase (NCBI) compare
b2055 wcaE +0.3 0.2 predicted glycosyl transferase (NCBI) compare
b0617 citD +0.3 1.2 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2792 yqcC +0.3 1.8 hypothetical protein (NCBI) compare
b1165 ymgA +0.3 0.3 hypothetical protein (NCBI) compare
b1361 ydaW +0.3 0.3 orf, hypothetical protein (VIMSS) compare
b1324 tpx +0.3 1.1 thiol peroxidase (NCBI) compare
b0759 galE +0.3 2.3 UDP-galactose-4-epimerase (VIMSS) compare
b4523 yciX +0.3 1.7 hypothetical protein (RefSeq) compare
b1919 yedO +0.3 1.0 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1735 chbR +0.3 1.3 DNA-binding transcriptional dual regulator (NCBI) compare
b1690 ydiM +0.3 0.9 predicted transporter (NCBI) compare
b3684 yidP +0.3 2.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0717 ybgP +0.3 1.8 predicted assembly protein (NCBI) compare
b1013 ycdC +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4511 ybdZ +0.3 1.9 hypothetical protein (NCBI) compare
b0865 ybjP +0.3 1.7 predicted lipoprotein (NCBI) compare
b0377 sbmA +0.3 2.8 predicted transporter (NCBI) compare
b3195 yrbF +0.3 2.6 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0696 kdpC +0.3 1.5 potassium-transporting ATPase subunit C (NCBI) compare
b4032 malG +0.3 2.5 maltose transporter subunit (NCBI) compare
b2081 yegQ +0.3 2.4 predicted peptidase (NCBI) compare
b0279 yagM +0.3 0.2 CP4-6 prophage; predicted protein (NCBI) compare
b2136 yohD +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4522 yciX_1 +0.3 0.8 hypothetical protein (NCBI) compare
b0828 iaaA +0.3 1.3 L-asparaginase (NCBI) compare
b4458 oxyS +0.3 0.9 global regulatory RNA (NCBI) compare
b1741 ydjQ +0.3 1.8 endonuclease of nucleotide excision repair (NCBI) compare
b1111 ycfQ +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b2618 b2618 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1733 chbG +0.3 1.1 hypothetical protein (NCBI) compare
b4453 ldrD +0.3 1.2 toxic polypeptide, small (NCBI) compare
b3193 yrbD +0.3 2.5 predicted ABC-type organic solvent transporter (NCBI) compare
b1021 ycdP +0.3 1.9 predicted inner membrane protein (NCBI) compare
b2204 napH +0.3 1.3 quinol dehydrogenase membrane component (NCBI) compare
b1503 ydeR +0.3 1.2 predicted fimbrial-like adhesin protein (NCBI) compare
b1951 rcsA +0.3 0.6 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b0643 ybeL +0.3 2.0 hypothetical protein (NCBI) compare
b1029 ycdU +0.3 2.0 predicted inner membrane protein (NCBI) compare
b3443 yrhA +0.3 0.6 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 5 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms