Experiment set53IT074 for Escherichia coli BW25113

Compare to:

BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr

200 most important genes:

  gene name fitness t score description  
b2808 gcvA -4.2 -4.0 DNA-binding transcriptional dual regulator (NCBI) compare
b3860 dsbA -3.5 -7.1 periplasmic protein disulfide isomerase I (NCBI) compare
b4242 mgtA -3.3 -2.2 magnesium transporter (NCBI) compare
b1185 dsbB -2.9 -4.6 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0683 fur -2.8 -1.8 ferric uptake regulator (NCBI) compare
b4702 mgtL -2.8 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b0222 lpcA -2.8 -1.9 phosphoheptose isomerase (NCBI) compare
b3844 fre -2.6 -10.0 NAD(P)H-flavin reductase (NCBI) compare
b4171 miaA -2.6 -4.2 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1855 lpxM -2.6 -6.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b2017 yefM -2.5 -1.4 orf, hypothetical protein (VIMSS) compare
b2318 truA -2.5 -4.5 tRNA pseudouridine synthase A (NCBI) compare
b2312 purF -2.3 -8.1 amidophosphoribosyltransferase (NCBI) compare
b0439 lon -2.1 -3.5 DNA-binding ATP-dependent protease La (NCBI) compare
b0438 clpX -2.1 -2.5 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1235 rssB -2.0 -6.9 response regulator of RpoS (NCBI) compare
b3916 pfkA -2.0 -3.7 6-phosphofructokinase (NCBI) compare
b4005 purD -2.0 -11.1 phosphoribosylamine--glycine ligase (NCBI) compare
b3052 rfaE -2.0 -2.9 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1438 ydcQ -2.0 -3.0 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0080 fruR -2.0 -7.0 DNA-binding transcriptional dual regulator (NCBI) compare
b3493 pitA -1.9 -8.2 phosphate transporter, low-affinity (NCBI) compare
b3979 thrT -1.9 -0.7 tRNA-Thr (NCBI) compare
b2620 smpB -1.8 -1.7 SsrA-binding protein (NCBI) compare
b0145 dksA -1.8 -3.5 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0632 dacA -1.7 -9.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b4375 prfC -1.7 -6.8 peptide chain release factor 3 (NCBI) compare
b2330 yfcB -1.7 -3.2 putative adenine-specific methylase (VIMSS) compare
b3183 obgE -1.7 -2.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4006 purH -1.7 -10.7 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b1292 sapC -1.7 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2414 cysK -1.6 -9.9 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b0726 sucA -1.6 -5.9 alpha-ketoglutarate decarboxylase (NCBI) compare
b0437 clpP -1.6 -1.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3162 deaD -1.6 -5.9 inducible ATP-independent RNA helicase (VIMSS) compare
b2501 ppk -1.6 -10.2 polyphosphate kinase (NCBI) compare
b0049 apaH -1.5 -1.0 diadenosinetetraphosphatase (NCBI) compare
b4203 rplI -1.5 -7.2 50S ribosomal protein L9 (NCBI) compare
b0522 purK -1.5 -7.5 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b3164 pnp -1.5 -3.6 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3870 glnA -1.5 -3.5 glutamine synthetase (NCBI) compare
b3838 tatB -1.4 -6.6 sec-independent translocase (NCBI) compare
b0400 phoR -1.4 -10.2 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b3849 trkH -1.4 -10.1 potassium transporter (NCBI) compare
b1850 eda -1.4 -2.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0200 gmhB -1.4 -0.9 hypothetical protein (NCBI) compare
b3630 rfaP -1.4 -5.7 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3621 rfaC -1.4 -1.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3204 ptsN -1.3 -5.3 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2329 aroC -1.3 -5.2 chorismate synthase (NCBI) compare
b2279 nuoK -1.3 -2.0 NADH dehydrogenase subunit K (NCBI) compare
b0226 dinJ -1.3 -3.9 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b2512 yfgL -1.3 -5.2 protein assembly complex, lipoprotein component (NCBI) compare
b0688 pgm -1.3 -3.2 phosphoglucomutase (NCBI) compare
b3620 rfaF -1.3 -2.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1291 sapD -1.3 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3792 wzxE -1.3 -2.2 O-antigen translocase (NCBI) compare
b4180 rlmB -1.3 -8.1 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b3631 rfaG -1.2 -7.7 glucosyltransferase I (NCBI) compare
b4654 yjdQ -1.2 -4.2 no description compare
b0051 ksgA -1.2 -4.4 dimethyladenosine transferase (NCBI) compare
b3867 hemN -1.2 -8.8 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b2784 relA -1.2 -8.8 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b0908 aroA -1.2 -6.8 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1084 rne -1.1 -3.8 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b1290 sapF -1.1 -1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b2421 cysM -1.1 -4.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0630 lipB -1.1 -1.4 protein of lipoate biosynthesis (VIMSS) compare
b3842 rfaH -1.1 -3.4 transcriptional activator RfaH (NCBI) compare
b4481 rffT -1.1 -3.1 4-alpha-L-fucosyltransferase (NCBI) compare
b4175 hflC -1.1 -7.2 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2313 cvpA -1.1 -2.2 membrane protein required for colicin V production (NCBI) compare
b3414 gntY -1.0 -5.4 predicted gluconate transport associated protein (NCBI) compare
b4676 yoaK -1.0 -0.8 expressed protein (RefSeq) compare
b1332 ynaJ -1.0 -1.1 predicted inner membrane protein (NCBI) compare
b2569 lepA -1.0 -3.5 GTP-binding protein LepA (NCBI) compare
b0386 proC -1.0 -2.5 pyrroline-5-carboxylate reductase (NCBI) compare
b1176 minC -1.0 -1.9 septum formation inhibitor (NCBI) compare
b2280 nuoJ -1.0 -4.7 NADH dehydrogenase subunit J (NCBI) compare
b3387 dam -1.0 -5.0 DNA adenine methylase (NCBI) compare
b2278 nuoL -1.0 -6.4 NADH dehydrogenase subunit L (NCBI) compare
b2935 tktA -1.0 -1.4 transketolase 1, thiamin-binding (NCBI) compare
b4179 vacB -1.0 -4.0 putative enzyme (VIMSS) compare
b0912 ihfB -1.0 -0.6 integration host factor subunit beta (NCBI) compare
b2277 nuoM -1.0 -5.1 NADH dehydrogenase subunit M (NCBI) compare
b0928 aspC -1.0 -5.6 aspartate aminotransferase, PLP-dependent (NCBI) compare
b2175 spr -1.0 -2.8 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3791 rffA -1.0 -3.4 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1107 nagZ -1.0 -4.1 beta-hexosaminidase (NCBI) compare
b3236 mdh -1.0 -5.5 malate dehydrogenase (NCBI) compare
b1982 amn -0.9 -6.8 AMP nucleosidase (NCBI) compare
b2283 nuoG -0.9 -5.7 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2284 nuoF -0.9 -4.0 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b3642 pyrE -0.9 -1.8 orotate phosphoribosyltransferase (NCBI) compare
b0767 ybhE -0.9 -2.9 6-phosphogluconolactonase (NCBI) compare
b4174 hflK -0.9 -5.9 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1192 ldcA -0.9 -2.2 L,D-carboxypeptidase A (NCBI) compare
b1346 ydaQ -0.9 -1.5 orf, hypothetical protein (VIMSS) compare
b3806 cyaA -0.9 -3.8 adenylate cyclase (NCBI) compare
b2276 nuoN -0.9 -3.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b4025 pgi -0.9 -5.8 glucose-6-phosphate isomerase (NCBI) compare
b2553 glnB -0.9 -5.1 regulatory protein P-II for glutamine synthetase (NCBI) compare
b0564 appY -0.9 -0.8 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3787 rffD -0.8 -5.3 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4146 yjeK -0.8 -1.8 predicted lysine aminomutase (NCBI) compare
b1613 manA -0.8 -1.8 mannose-6-phosphate isomerase (VIMSS) compare
b3977 tyrU -0.8 -0.8 tRNA-Tyr (NCBI) compare
b3741 gidA -0.8 -1.7 glucose-inhibited division protein A (NCBI) compare
b3545 proK -0.8 -2.2 tRNA-Pro (NCBI) compare
b1551 b1551 -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b2282 nuoH -0.8 -2.9 NADH dehydrogenase subunit H (NCBI) compare
b2340 sixA -0.8 -3.2 phosphohistidine phosphatase (NCBI) compare
b0889 lrp -0.8 -1.8 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3786 rffE -0.8 -5.9 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1025 ycdT -0.8 -1.8 predicted diguanylate cyclase (NCBI) compare
b1286 rnb -0.8 -1.8 exoribonuclease II (NCBI) compare
b4458 oxyS -0.8 -1.5 global regulatory RNA (NCBI) compare
b2617 smpA -0.8 -3.0 small membrane protein A (VIMSS) compare
b2281 nuoI -0.8 -2.4 NADH dehydrogenase subunit I (NCBI) compare
b0722 sdhD -0.8 -3.4 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0142 folK -0.8 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2683 ygaH -0.8 -2.9 predicted inner membrane protein (NCBI) compare
b3935 priA -0.8 -2.6 primosome assembly protein PriA (NCBI) compare
b3778 rep -0.8 -4.3 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b0926 ycbK -0.8 -3.8 hypothetical protein (NCBI) compare
b2286 nuoC -0.8 -3.4 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b4201 priB -0.8 -2.5 primosomal replication protein N (NCBI) compare
b4414 tff -0.8 -1.3 unknown RNA (NCBI) compare
b1236 galU -0.8 -1.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2906 visC -0.8 -4.7 hypothetical protein (NCBI) compare
b0723 sdhA -0.8 -6.3 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b4245 pyrB -0.7 -2.1 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0113 pdhR -0.7 -4.3 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b4041 plsB -0.7 -1.7 glycerol-3-phosphate acyltransferase (VIMSS) compare
b3237 argR -0.7 -2.8 arginine repressor (NCBI) compare
b3706 trmE -0.7 -1.9 tRNA modification GTPase (NCBI) compare
b3205 yhbJ -0.7 -3.1 hypothetical protein (NCBI) compare
b4647 mokA -0.7 -0.8 no description compare
b1961 dcm -0.7 -1.9 DNA cytosine methylase (NCBI) compare
b1231 tyrT -0.7 -1.0 tRNA-Tyr (NCBI) compare
b0550 rusA -0.7 -2.5 DLP12 prophage; endonuclease RUS (NCBI) compare
b3601 mtlR -0.7 -4.4 DNA-binding repressor (NCBI) compare
b1418 cybB -0.7 -3.6 cytochrome b(561) (VIMSS) compare
b2188 yejM -0.7 -4.8 predicted hydrolase, inner membrane (NCBI) compare
b3627 rfaI -0.7 -5.0 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1676 pykF -0.7 -3.8 pyruvate kinase (NCBI) compare
b4484 cpxP -0.7 -3.4 periplasmic protein combats stress (NCBI) compare
b0783 moaC -0.7 -3.5 molybdenum cofactor biosynthesis protein C (NCBI) compare
b3965 trmA -0.7 -6.0 tRNA (uracil-5-)-methyltransferase (NCBI) compare
b0677 nagA -0.7 -3.8 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b4139 aspA -0.7 -5.3 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b2557 purL -0.7 -4.9 phosphoribosylformylglycinamidine synthase (NCBI) compare
b0053 surA -0.7 -0.8 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0764 modB -0.7 -3.8 molybdate ABC transporter permease protein (NCBI) compare
b1412 azoR -0.7 -2.1 acyl carrier protein phosphodiesterase (NCBI) compare
b2905 gcvT -0.7 -4.2 glycine cleavage system aminomethyltransferase T (NCBI) compare
b3764 yifE -0.7 -3.5 hypothetical protein (NCBI) compare
b2912 ygfA -0.7 -1.4 putative ligase (VIMSS) compare
b4388 serB -0.7 -4.8 3-phosphoserine phosphatase (NCBI) compare
b0143 pcnB -0.7 -3.5 poly(A) polymerase I (VIMSS) compare
b4698 mgrR -0.7 -3.0 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b3247 cafA -0.7 -5.2 bundles of cytoplasmic filaments (VIMSS) compare
b0209 yafD -0.7 -3.3 hypothetical protein (NCBI) compare
b2669 stpA -0.7 -5.5 DNA binding protein, nucleoid-associated (NCBI) compare
b4000 hupA -0.7 -4.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1951 rcsA -0.7 -1.2 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2518 ndk -0.7 -1.6 nucleoside diphosphate kinase (NCBI) compare
b0548 ninE -0.6 -2.7 DLP12 prophage; conserved protein (NCBI) compare
b3652 recG -0.6 -4.6 ATP-dependent DNA helicase (NCBI) compare
b4675 yoaJ -0.6 -2.1 expressed protein (RefSeq) compare
b3794 rffM -0.6 -3.1 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3930 menA -0.6 -5.7 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b3398 yrfF -0.6 -1.9 predicted inner membrane protein (NCBI) compare
b2493 yfgO -0.6 -4.7 predicted inner membrane protein (NCBI) compare
b4593 ymgI -0.6 -0.7 hypothetical protein (RefSeq) compare
b0948 ycbY -0.6 -4.3 predicted methyltransferase (NCBI) compare
b1865 nudB -0.6 -0.7 dATP pyrophosphohydrolase (NCBI) compare
b0882 clpA -0.6 -5.0 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b1293 sapB -0.6 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3911 cpxA -0.6 -3.2 two-component sensor protein (NCBI) compare
b4243 yjgF -0.6 -3.2 orf, hypothetical protein (VIMSS) compare
b2498 upp -0.6 -1.6 uracil phosphoribosyltransferase (VIMSS) compare
b0693 speF -0.6 -4.2 ornithine decarboxylase isozyme, inducible (NCBI) compare
b0724 sdhB -0.6 -3.5 succinate dehydrogenase, FeS subunit (NCBI) compare
b2168 fruK -0.6 -0.6 1-phosphofructokinase (NCBI) compare
b3781 trxA -0.6 -3.1 thioredoxin 1 (VIMSS) compare
b1863 ruvC -0.6 -0.4 Holliday junction resolvase (NCBI) compare
b0032 carA -0.6 -2.3 carbamoyl-phosphate synthase small subunit (NCBI) compare
b3869 glnL -0.6 -3.1 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b3281 aroE -0.6 -4.8 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0706 ybfD -0.6 -1.9 hypothetical protein (NCBI) compare
b3494 yhiO -0.6 -3.6 universal stress protein UspB (NCBI) compare
b2904 gcvH -0.6 -1.8 glycine cleavage system protein H (NCBI) compare
b2516 yfgA -0.6 -3.5 hypothetical protein (NCBI) compare
b4390 nadR -0.6 -4.6 probable nadAB transcriptional regulator (VIMSS) compare
b3628 rfaB -0.6 -4.4 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b4547 ypfN -0.6 -2.5 hypothetical protein (NCBI) compare
b3389 aroB -0.6 -3.9 3-dehydroquinate synthase (NCBI) compare
b3289 rsmB -0.6 -4.9 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in no stress control experiments

For no stress control Dimethyl Sulfoxide across organisms