Experiment set53IT061 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.06 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b2741 rpoS +1.3 10.3 RNA polymerase sigma factor (NCBI) compare
b2934 cmtB +1.3 1.8 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2620 smpB +1.3 2.3 SsrA-binding protein (NCBI) compare
b1936 intG +1.3 2.0 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3210 arcB +1.3 9.2 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1361 ydaW +1.0 1.1 orf, hypothetical protein (VIMSS) compare
b1085 b1085 +1.0 2.6 orf, hypothetical protein (VIMSS) compare
b3387 dam +0.9 6.3 DNA adenine methylase (NCBI) compare
b3844 fre +0.8 5.0 NAD(P)H-flavin reductase (NCBI) compare
b4313 fimE +0.8 6.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1600 mdtJ +0.8 2.0 multidrug efflux system transporter (NCBI) compare
b4426 isrA +0.7 2.2 unknown RNA (NCBI) compare
b1954 dsrA +0.7 3.7 regulatory antisense RNA (NCBI) compare
b3146 yraL +0.7 4.8 predicted methyltransferase (NCBI) compare
b4590 ybfK +0.7 1.1 hypothetical protein (RefSeq) compare
b0082 mraW +0.7 4.2 S-adenosyl-methyltransferase (NCBI) compare
b2217 rcsB +0.7 4.6 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1823 cspC +0.7 1.9 stress protein, member of the CspA-family (NCBI) compare
b3163 nlpI +0.6 3.2 hypothetical protein (NCBI) compare
b4593 ymgI +0.6 1.0 hypothetical protein (RefSeq) compare
b4133 cadC +0.6 5.5 DNA-binding transcriptional activator (NCBI) compare
b2846 yqeH +0.6 0.6 orf, hypothetical protein (VIMSS) compare
b2272 yfbM +0.6 3.0 hypothetical protein (NCBI) compare
b4246 pyrL +0.6 0.9 pyrBI operon leader peptide (NCBI) compare
b4050 yjbO +0.6 3.7 orf, hypothetical protein (VIMSS) compare
b0131 panD +0.6 2.4 aspartate 1-decarboxylase precursor (NCBI) compare
b1179 ycgL +0.6 1.8 conserved protein (NCBI) compare
b3198 kdsC +0.6 2.3 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2684 mprA +0.5 3.1 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1253 yciA +0.5 1.6 predicted hydrolase (NCBI) compare
b3523 yhjE +0.5 4.7 predicted transporter (NCBI) compare
b2829 ptsP +0.5 5.1 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1919 yedO +0.5 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0240 crl +0.5 4.1 DNA-binding transcriptional regulator (NCBI) compare
b4571 wbbL +0.5 1.9 no description compare
b2531 iscR +0.5 1.6 DNA-binding transcriptional repressor (NCBI) compare
b0382 yaiB +0.5 4.1 hypothetical protein (NCBI) compare
b1355 b1355 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1269 rluB +0.5 2.9 23S rRNA pseudouridylate synthase (NCBI) compare
b2110 yehC +0.5 1.2 predicted periplasmic pilin chaperone (NCBI) compare
b2229 b2229 +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b4594 ymgJ +0.5 2.0 hypothetical protein (RefSeq) compare
b1233 ychJ +0.5 2.4 hypothetical protein (NCBI) compare
b3933 ftsN +0.5 3.9 essential cell division protein (NCBI) compare
b3261 fis +0.5 1.4 DNA-binding protein Fis (NCBI) compare
b3206 npr +0.5 3.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b3512 GadE +0.5 2.7 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1824 yobF +0.5 2.7 hypothetical protein (NCBI) compare
b1493 gadB +0.5 0.9 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4585 sroB +0.5 1.2 Novel sRNA, function unknown (RefSeq) compare
b1724 ydiZ +0.5 1.4 hypothetical protein (NCBI) compare
b1591 b1591 +0.5 1.5 putative oxidoreductase component (VIMSS) compare
b3442 yhhZ +0.5 0.3 hypothetical protein (NCBI) compare
b1575 dicB +0.5 1.4 Qin prophage; cell division inhibition protein (NCBI) compare
b1159 mcrA +0.5 1.0 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2436 hemF +0.5 3.3 coproporphyrinogen III oxidase (NCBI) compare
b1161 ycgX +0.5 0.9 hypothetical protein (NCBI) compare
b1830 prc +0.5 2.1 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0661 miaB +0.5 4.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3443 yrhA +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b2688 gshA +0.5 3.0 glutamate--cysteine ligase (NCBI) compare
b3753 rbsR +0.4 4.1 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0955 ycbZ +0.4 3.5 predicted peptidase (NCBI) compare
b2236 yfaE +0.4 2.3 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2286 nuoC +0.4 2.7 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1246 oppD +0.4 3.3 oligopeptide transporter ATP-binding component (NCBI) compare
b1186 nhaB +0.4 3.1 NhaB sodium/proton transporter (Katherine Huang) compare
b1527 yneK +0.4 1.5 hypothetical protein (NCBI) compare
b1597 asr +0.4 1.9 acid shock protein (VIMSS) compare
b3816 corA +0.4 3.5 magnesium/nickel/cobalt transporter (NCBI) compare
b3755 yieP +0.4 3.4 predicted transcriptional regulator (NCBI) compare
b1628 rsxB +0.4 2.1 electron transport complex protein RnfB (NCBI) compare
b3213 gltD +0.4 3.3 glutamate synthase, 4Fe-4S protein, small subunit (NCBI) compare
b2285 nuoE +0.4 1.3 NADH dehydrogenase subunit E (NCBI) compare
b1613 manA +0.4 1.0 mannose-6-phosphate isomerase (VIMSS) compare
b3349 slyD +0.4 3.0 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b4532 yncN +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b0736 ybgC +0.4 2.1 predicted acyl-CoA thioesterase (NCBI) compare
b0357 yaiN +0.4 1.2 putative alpha helix chain (VIMSS) compare
b1380 ldhA +0.4 1.9 D-lactate dehydrogenase (NCBI) compare
b4222 ytfP +0.4 2.0 hypothetical protein (NCBI) compare
b1553 ydfP +0.4 1.1 Qin prophage; conserved protein (NCBI) compare
b1670 ydhU +0.4 2.3 predicted cytochrome (NCBI) compare
b0685 ybfE +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1679 sufE +0.4 2.2 cysteine desufuration protein SufE (NCBI) compare
b1247 oppF +0.4 2.8 oligopeptide transporter subunit (NCBI) compare
b3884 yihW +0.4 2.3 putative DEOR-type transcriptional regulator (VIMSS) compare
b1382 ynbE +0.4 1.2 predicted lipoprotein (NCBI) compare
b0821 ybiU +0.4 2.3 hypothetical protein (NCBI) compare
b0232 yafN +0.4 0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4513 kdpF +0.4 1.9 potassium ion accessory transporter subunit (NCBI) compare
b2947 gshB +0.4 1.6 glutathione synthetase (NCBI) compare
b1335 ogt +0.4 2.0 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b4071 nrfB +0.4 1.7 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b4552 yrhC +0.4 0.6 no description compare
b1642 slyA +0.4 1.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4692 oweE +0.4 2.0 no description compare
b2512 yfgL +0.4 1.8 protein assembly complex, lipoprotein component (NCBI) compare
b4397 creA +0.4 2.3 hypothetical protein (NCBI) compare
b2369 evgA +0.4 1.7 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b0849 grxA +0.4 1.7 glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI) compare
b2659 b2659 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b3292 zntR +0.4 1.9 zinc-responsive transcriptional regulator (NCBI) compare
b4101 phnG +0.4 1.4 carbon-phosphorus lyase complex subunit (NCBI) compare
b1761 gdhA +0.4 2.2 glutamate dehydrogenase (NCBI) compare
b1007 b1007 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0696 kdpC +0.3 1.4 potassium-transporting ATPase subunit C (NCBI) compare
b3341 rpsG +0.3 0.7 30S ribosomal protein S7 (NCBI) compare
b4559 yjdO +0.3 1.7 hypothetical protein (NCBI) compare
b0858 ybjO +0.3 1.5 predicted inner membrane protein (NCBI) compare
b3181 greA +0.3 1.7 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1492 gadC +0.3 2.6 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1992 cobS +0.3 0.9 cobalamin synthase (NCBI) compare
b0425 panE +0.3 2.8 2-dehydropantoate 2-reductase (NCBI) compare
b1623 add +0.3 1.6 adenosine deaminase (NCBI) compare
b1422 ydcI +0.3 1.8 putative transcriptional regulator LYSR-type (VIMSS) compare
b1557 cspB +0.3 1.2 Qin prophage; cold shock protein (NCBI) compare
b2284 nuoF +0.3 1.5 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1950 fliR +0.3 1.5 flagellar biosynthesis protein R (NCBI) compare
b3495 uspA +0.3 2.7 universal stress global response regulator (NCBI) compare
b2083 yegZ +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1625 ydgT +0.3 2.3 predicted regulator (NCBI) compare
b0503 ybbB +0.3 1.5 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b2749 ygbE +0.3 2.4 conserved inner membrane protein (NCBI) compare
b2377 yfdY +0.3 1.3 predicted inner membrane protein (NCBI) compare
b1218 chaC +0.3 2.2 regulatory protein for cation transport (RefSeq) compare
b2998 yghW +0.3 1.6 hypothetical protein (NCBI) compare
b1999 yeeP +0.3 2.1 putative histone (VIMSS) compare
b1346 ydaQ +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b2968 yghD +0.3 2.0 predicted secretion pathway M-type protein, membrane anchored (NCBI) compare
b1482 osmC +0.3 1.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b2586 yfiM +0.3 1.6 predicted protein (RefSeq) compare
b2859 ygeO +0.3 1.0 hypothetical protein (RefSeq) compare
b3658 selC +0.3 1.5 tRNA-Sec (NCBI) compare
b2349 intS +0.3 2.4 CPS-53 (KpLE1) prophage; predicted prophage CPS-53 integrase (NCBI) compare
b1182 hlyE +0.3 0.3 hemolysin E (VIMSS) compare
b3676 yidH +0.3 1.7 conserved inner membrane protein (NCBI) compare
b1481 b1481 +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1330 ynaI +0.3 1.7 conserved inner membrane protein (NCBI) compare
b1958 yedI +0.3 2.2 conserved inner membrane protein (NCBI) compare
b1692 ydiB +0.3 1.4 quinate/shikimate 5-dehydrogenase, NAD(P)-binding (NCBI) compare
b0178 hlpA +0.3 1.8 periplasmic chaperone (NCBI) compare
b0122 yacC +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2212 alkB +0.3 1.6 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b1025 ycdT +0.3 0.8 predicted diguanylate cyclase (NCBI) compare
b4536 yobH +0.3 1.7 hypothetical protein (NCBI) compare
b1726 yniB +0.3 0.9 predicted inner membrane protein (NCBI) compare
b2007 yeeX +0.3 0.6 conserved protein (RefSeq) compare
b1278 pgpB +0.3 1.2 phosphatidylglycerophosphatase B (NCBI) compare
b2502 ppx +0.3 2.1 exopolyphosphatase (NCBI) compare
b0987 ymcD +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b1244 oppB +0.3 2.0 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b0432 cyoA +0.3 2.4 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b2485 hyfE +0.3 1.9 hydrogenase 4, membrane subunit (NCBI) compare
b3212 gltB +0.3 2.7 glutamate synthase, large subunit (NCBI) compare
b0120 speD +0.3 2.4 S-adenosylmethionine decarboxylase proenzyme (NCBI) compare
b1524 yneH +0.3 0.6 predicted glutaminase (NCBI) compare
b1630 rsxD +0.3 1.4 electron transport complex protein RnfD (NCBI) compare
b0782 moaB +0.3 0.7 molybdopterin biosynthesis protein B (NCBI) compare
b0616 citE +0.3 2.0 citrate lyase beta chain (acyl lyase subunit) (VIMSS) compare
b4441 glmY +0.3 0.9 sRNA activator of glmS mRNA (RefSeq) compare
b2204 napH +0.3 1.5 quinol dehydrogenase membrane component (NCBI) compare
b0136 yadK +0.3 1.4 predicted fimbrial-like adhesin protein (NCBI) compare
b0976 hyaE +0.3 1.7 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b3769 ilvM +0.3 1.2 acetolactate synthase II, small subunit (NCBI) compare
b3871 bipA +0.3 2.3 GTP-binding protein (NCBI) compare
b2782 chpA +0.3 1.8 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b2571 rseB +0.3 2.2 periplasmic negative regulator of sigmaE (NCBI) compare
b1594 dgsA +0.3 1.2 DNA-binding transcriptional repressor (NCBI) compare
b1674 ydhY +0.3 2.1 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b0851 nfsA +0.3 1.3 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1394 paaG +0.3 1.0 enoyl-CoA hydratase (NCBI) compare
b1900 araG +0.3 1.4 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1743 spy +0.3 1.0 envelope stress induced periplasmic protein (NCBI) compare
b4650 yibS +0.3 1.0 no description compare
b0925 ycbB +0.3 2.2 predicted carboxypeptidase (NCBI) compare
b1258 yciF +0.3 0.5 hypothetical protein (NCBI) compare
b4048 yjbM +0.3 0.6 hypothetical protein (NCBI) compare
b4642 yoeD +0.3 1.2 no description compare
b2421 cysM +0.3 0.9 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b3672 ivbL +0.3 1.2 ilvB operon leader peptide (NCBI) compare
b2635 ypjK +0.3 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1728 ydjM +0.3 0.9 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1681 sufD +0.3 1.9 component of SufBCD complex (NCBI) compare
b1409 ynbB +0.3 0.5 predicted CDP-diglyceride synthase (NCBI) compare
b0535 fimZ +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b0989 cspH +0.3 0.9 stress protein, member of the CspA-family (NCBI) compare
b3071 yqjI +0.3 1.2 predicted transcriptional regulator (NCBI) compare
b0279 yagM +0.3 0.2 CP4-6 prophage; predicted protein (NCBI) compare
b4049 dusA +0.3 2.2 tRNA-dihydrouridine synthase A (RefSeq) compare
b1465 narV +0.3 0.8 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b3075 ebgR +0.3 1.5 DNA-binding transcriptional repressor (NCBI) compare
b3888 yiiD +0.3 2.0 predicted acetyltransferase (NCBI) compare
b2294 b2294 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b1245 oppC +0.2 1.8 oligopeptide transporter subunit (NCBI) compare
b2195 ccmG +0.2 0.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0753 ybgS +0.2 1.7 hypothetical protein (NCBI) compare
b0468 ybaN +0.2 1.7 conserved inner membrane protein (NCBI) compare
b3169 nusA +0.2 0.5 transcription elongation factor NusA (NCBI) compare
b0469 apt +0.2 1.8 adenine phosphoribosyltransferase (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms