Experiment set53IT058 for Escherichia coli BW25113

Compare to:

M9 no stress control; Anaerobic 48 hr

200 most important genes:

  gene name fitness t score description  
b0928 aspC -5.0 -10.9 aspartate aminotransferase, PLP-dependent (NCBI) compare
b3956 ppc -4.8 -26.0 phosphoenolpyruvate carboxylase (NCBI) compare
b2838 lysA -4.7 -18.7 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b0004 thrC -4.7 -12.9 threonine synthase (NCBI) compare
b0003 thrB -4.7 -10.7 homoserine kinase (NCBI) compare
b0907 serC -4.7 -9.9 phosphoserine aminotransferase (NCBI) compare
b2476 purC -4.6 -3.7 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b0002 thrA -4.4 -21.5 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b4006 purH -4.4 -12.9 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b0523 purE -4.4 -5.2 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b1241 adhE -4.3 -17.2 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b2784 relA -4.3 -17.6 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b4005 purD -4.3 -12.8 phosphoribosylamine--glycine ligase (NCBI) compare
b2557 purL -4.2 -14.1 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2913 serA -4.2 -16.2 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b4025 pgi -4.2 -14.8 glucose-6-phosphate isomerase (NCBI) compare
b4388 serB -4.1 -8.8 3-phosphoserine phosphatase (NCBI) compare
b2329 aroC -4.0 -10.3 chorismate synthase (NCBI) compare
b0908 aroA -4.0 -14.5 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1185 dsbB -4.0 -2.7 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2501 ppk -3.9 -14.0 polyphosphate kinase (NCBI) compare
b3860 dsbA -3.9 -3.8 periplasmic protein disulfide isomerase I (NCBI) compare
b0902 pflA -3.9 -11.9 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b1850 eda -3.5 -4.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b3960 argH -3.5 -19.7 argininosuccinate lyase (NCBI) compare
b1261 trpB -3.5 -6.6 tryptophan synthase subunit beta (NCBI) compare
b0903 pflB -3.4 -17.2 pyruvate formate lyase I (NCBI) compare
b0720 gltA -3.4 -9.3 citrate synthase (NCBI) compare
b0889 lrp -3.4 -5.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2312 purF -3.3 -8.4 amidophosphoribosyltransferase (NCBI) compare
b2499 purM -3.3 -6.6 phosphoribosylaminoimidazole synthetase (NCBI) compare
b4237 nrdG -3.2 -13.8 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b0145 dksA -3.2 -3.1 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0522 purK -3.2 -10.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b0142 folK -3.1 -1.4 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3172 argG -3.1 -15.6 argininosuccinate synthase (NCBI) compare
b3930 menA -2.9 -18.7 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1062 pyrC -2.9 -4.8 dihydroorotase (NCBI) compare
b1865 nudB -2.9 -2.6 dATP pyrophosphohydrolase (NCBI) compare
b1290 sapF -2.9 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4238 nrdD -2.9 -18.2 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4242 mgtA -2.7 -8.0 magnesium transporter (NCBI) compare
b1260 trpA -2.7 -6.1 tryptophan synthase subunit alpha (NCBI) compare
b3916 pfkA -2.7 -2.6 6-phosphofructokinase (NCBI) compare
b1281 pyrF -2.7 -2.5 orotidine 5'-phosphate decarboxylase (NCBI) compare
b3642 pyrE -2.6 -1.8 orotate phosphoribosyltransferase (NCBI) compare
b1192 ldcA -2.6 -3.1 L,D-carboxypeptidase A (NCBI) compare
b2188 yejM -2.6 -14.2 predicted hydrolase, inner membrane (NCBI) compare
b0632 dacA -2.6 -11.0 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0688 pgm -2.6 -3.0 phosphoglucomutase (NCBI) compare
b4402 yjjY -2.5 -1.8 hypothetical protein (NCBI) compare
b3389 aroB -2.5 -13.0 3-dehydroquinate synthase (NCBI) compare
b3237 argR -2.5 -7.4 arginine repressor (NCBI) compare
b2175 spr -2.5 -5.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2762 cysH -2.4 -12.5 phosphoadenosine phosphosulfate reductase (NCBI) compare
b0222 lpcA -2.4 -2.3 phosphoheptose isomerase (NCBI) compare
b1677 lpp -2.4 -3.3 murein lipoprotein (NCBI) compare
b0052 pdxA -2.4 -15.0 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b3911 cpxA -2.4 -9.1 two-component sensor protein (NCBI) compare
b3281 aroE -2.3 -15.9 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3870 glnA -2.3 -2.7 glutamine synthetase (NCBI) compare
b0945 pyrD -2.3 -2.2 dihydroorotate dehydrogenase (NCBI) compare
b2320 pdxB -2.3 -12.6 erythronate-4-phosphate dehydrogenase (NCBI) compare
b2839 lysR -2.3 -5.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2763 cysI -2.3 -12.2 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b4245 pyrB -2.2 -2.2 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b4702 mgtL -2.2 -2.1 regulatory leader peptide for mgtA (NCBI) compare
b4171 miaA -2.2 -4.3 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0728 sucC -2.1 -7.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b3781 trxA -2.1 -7.3 thioredoxin 1 (VIMSS) compare
b3229 sspA -2.1 -5.5 stringent starvation protein A (NCBI) compare
b2764 cysJ -2.1 -10.6 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b3941 metF -2.1 -14.4 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b2564 pdxJ -2.1 -8.3 pyridoxal phosphate biosynthetic protein (NCBI) compare
b0088 murD -2.1 -4.2 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b1693 aroD -2.0 -7.6 3-dehydroquinate dehydratase (NCBI) compare
b3368 cysG -2.0 -16.0 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b0438 clpX -2.0 -3.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3164 pnp -2.0 -4.2 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2313 cvpA -2.0 -5.0 membrane protein required for colicin V production (NCBI) compare
b3829 metE -2.0 -15.2 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b0032 carA -2.0 -3.9 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0564 appY -1.9 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1687 ydiJ -1.9 -10.8 predicted FAD-linked oxidoreductase (NCBI) compare
b3052 rfaE -1.9 -2.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3979 thrT -1.9 -0.8 tRNA-Thr (NCBI) compare
b2023 hisH -1.9 -9.8 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3828 metR -1.8 -11.3 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b1101 ptsG -1.8 -12.1 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b0729 sucD -1.8 -4.3 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3838 tatB -1.8 -8.1 sec-independent translocase (NCBI) compare
b0242 proB -1.8 -8.2 gamma-glutamyl kinase (NCBI) compare
b2024 hisA -1.8 -5.1 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2414 cysK -1.8 -10.6 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b1235 rssB -1.8 -6.4 response regulator of RpoS (NCBI) compare
b2750 cysC -1.8 -8.5 adenylylsulfate kinase (NCBI) compare
b0767 ybhE -1.7 -5.3 6-phosphogluconolactonase (NCBI) compare
b3791 rffA -1.7 -4.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3493 pitA -1.7 -7.5 phosphate transporter, low-affinity (NCBI) compare
b0439 lon -1.7 -4.0 DNA-binding ATP-dependent protease La (NCBI) compare
b3620 rfaF -1.7 -2.9 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2022 hisB -1.7 -9.6 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b2912 ygfA -1.7 -2.6 putative ligase (VIMSS) compare
b2021 hisC -1.7 -9.2 histidinol-phosphate aminotransferase (NCBI) compare
b2600 tyrA -1.6 -5.8 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b0926 ycbK -1.6 -4.3 hypothetical protein (NCBI) compare
b1334 fnr -1.6 -4.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2025 hisF -1.6 -7.4 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b4481 rffT -1.6 -4.1 4-alpha-L-fucosyltransferase (NCBI) compare
b4146 yjeK -1.6 -1.5 predicted lysine aminomutase (NCBI) compare
b3774 ilvC -1.6 -13.6 ketol-acid reductoisomerase (NCBI) compare
b3755 yieP -1.6 -7.2 predicted transcriptional regulator (NCBI) compare
b2017 yefM -1.6 -1.2 orf, hypothetical protein (VIMSS) compare
b1613 manA -1.5 -2.7 mannose-6-phosphate isomerase (VIMSS) compare
b0033 carB -1.5 -2.7 carbamoyl-phosphate synthase large subunit (NCBI) compare
b3790 rffC -1.5 -3.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b2752 cysD -1.5 -5.8 sulfate adenylyltransferase subunit 2 (NCBI) compare
b0437 clpP -1.5 -1.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1593 b1593 -1.5 -4.4 orf, hypothetical protein (VIMSS) compare
b1291 sapD -1.5 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0386 proC -1.5 -2.9 pyrroline-5-carboxylate reductase (NCBI) compare
b2020 hisD -1.5 -7.8 histidinol dehydrogenase (NCBI) compare
b3770 ilvE -1.5 -10.9 branched-chain amino acid aminotransferase (NCBI) compare
b0113 pdhR -1.5 -5.4 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b3621 rfaC -1.4 -1.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3771 ilvD -1.4 -11.2 dihydroxy-acid dehydratase (NCBI) compare
b2318 truA -1.4 -3.3 tRNA pseudouridine synthase A (NCBI) compare
b2019 hisG -1.4 -6.4 ATP phosphoribosyltransferase (NCBI) compare
b3958 argC -1.4 -9.7 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b4214 cysQ -1.4 -10.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0400 phoR -1.4 -9.4 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b0957 ompA -1.4 -4.7 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3741 gidA -1.3 -1.8 glucose-inhibited division protein A (NCBI) compare
b0741 pal -1.3 -1.5 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1332 ynaJ -1.3 -1.2 predicted inner membrane protein (NCBI) compare
b3429 glgA -1.3 -9.6 glycogen synthase (NCBI) compare
b0425 panE -1.3 -8.2 2-dehydropantoate 2-reductase (NCBI) compare
b3205 yhbJ -1.3 -4.8 hypothetical protein (NCBI) compare
b0619 citA -1.3 -5.6 sensory histidine kinase in two-component regulatory system with citB (NCBI) compare
b0232 yafN -1.3 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3792 wzxE -1.3 -2.3 O-antigen translocase (NCBI) compare
b3787 rffD -1.2 -7.2 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3772 ilvA -1.2 -10.5 threonine dehydratase (NCBI) compare
b2330 yfcB -1.2 -2.3 putative adenine-specific methylase (VIMSS) compare
b0243 proA -1.2 -7.0 gamma-glutamyl phosphate reductase (NCBI) compare
b3786 rffE -1.2 -8.3 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1111 ycfQ -1.2 -3.0 orf, hypothetical protein (VIMSS) compare
b3212 gltB -1.2 -10.7 glutamate synthase, large subunit (NCBI) compare
b0077 ilvI -1.2 -9.5 acetolactate synthase III large subunit (NCBI) compare
b3957 argE -1.2 -10.0 acetylornithine deacetylase (NCBI) compare
b2751 cysN -1.2 -7.2 sulfate adenylyltransferase subunit 1 (NCBI) compare
b3842 rfaH -1.2 -3.6 transcriptional activator RfaH (NCBI) compare
b3213 gltD -1.2 -8.0 glutamate synthase, 4Fe-4S protein, small subunit (NCBI) compare
b2808 gcvA -1.2 -4.1 DNA-binding transcriptional dual regulator (NCBI) compare
b1262 trpC -1.2 -6.8 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b0078 ilvH -1.2 -7.2 acetolactate synthase small subunit (NCBI) compare
b0073 leuB -1.2 -8.8 3-isopropylmalate dehydrogenase (VIMSS) compare
b3387 dam -1.1 -5.4 DNA adenine methylase (NCBI) compare
b1686 ydiI -1.1 -3.7 hypothetical protein (NCBI) compare
b0149 mrcB -1.1 -7.6 penicillin-binding protein 1b (NCBI) compare
b3794 rffM -1.1 -5.5 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0053 surA -1.1 -1.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1084 rne -1.1 -4.0 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0904 focA -1.1 -4.5 formate transporter (NCBI) compare
b1253 yciA -1.1 -2.3 predicted hydrolase (NCBI) compare
b2518 ndk -1.1 -2.8 nucleoside diphosphate kinase (NCBI) compare
b3959 argB -1.1 -8.3 acetylglutamate kinase (RefSeq) compare
b1438 ydcQ -1.1 -3.0 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3359 argD -1.1 -9.7 bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase (NCBI) compare
b4175 hflC -1.1 -7.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3204 ptsN -1.1 -4.1 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3545 proK -1.1 -3.0 tRNA-Pro (NCBI) compare
b1855 lpxM -1.1 -4.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4174 hflK -1.1 -7.6 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2026 hisI -1.1 -5.5 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b3252 yhdA -1.1 -8.3 conserved inner membrane protein (NCBI) compare
b4390 nadR -1.0 -7.8 probable nadAB transcriptional regulator (VIMSS) compare
b3169 nusA -1.0 -1.7 transcription elongation factor NusA (NCBI) compare
b1127 pepT -1.0 -5.5 peptidase T (NCBI) compare
b3631 rfaG -1.0 -6.6 glucosyltransferase I (NCBI) compare
b0072 leuC -1.0 -8.0 isopropylmalate isomerase large subunit (NCBI) compare
b0881 clpS -1.0 -3.0 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b2818 argA -1.0 -6.7 N-acetylglutamate synthase (NCBI) compare
b2008 yeeA -1.0 -5.5 conserved inner membrane protein (NCBI) compare
b0051 ksgA -1.0 -3.9 dimethyladenosine transferase (NCBI) compare
b2424 cysU -1.0 -3.2 sulfate/thiosulfate transporter subunit (NCBI) compare
b3494 yhiO -1.0 -6.0 universal stress protein UspB (NCBI) compare
b2422 cysA -1.0 -6.0 sulfate/thiosulfate transporter subunit (NCBI) compare
b1524 yneH -1.0 -1.3 predicted glutaminase (NCBI) compare
b2504 yfgG -1.0 -1.7 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3055 htrG -1.0 -5.3 predicted signal transduction protein (SH3 domain) (NCBI) compare
b4024 lysC -1.0 -6.5 aspartate kinase III (NCBI) compare
b2498 upp -0.9 -2.7 uracil phosphoribosyltransferase (VIMSS) compare
b1095 fabF -0.9 -5.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0912 ihfB -0.9 -0.6 integration host factor subunit beta (NCBI) compare
b4179 vacB -0.9 -3.7 putative enzyme (VIMSS) compare
b2156 lysP -0.9 -7.2 lysine transporter (NCBI) compare
b3630 rfaP -0.9 -4.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0071 leuD -0.9 -5.9 isopropylmalate isomerase small subunit (NCBI) compare
b0133 panC -0.9 -6.4 pantoate--beta-alanine ligase (NCBI) compare


Specific Phenotypes

For 10 genes in this experiment