Experiment set53IT054 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 1.98 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b1243 oppA +3.4 15.1 oligopeptide transporter subunit (NCBI) compare
b1246 oppD +3.4 14.8 oligopeptide transporter ATP-binding component (NCBI) compare
b1245 oppC +3.1 15.5 oligopeptide transporter subunit (NCBI) compare
b1247 oppF +3.1 12.6 oligopeptide transporter subunit (NCBI) compare
b1244 oppB +3.1 16.1 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2684 mprA +3.0 6.5 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2469 narQ +2.8 5.3 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b1095 fabF +2.4 8.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3911 cpxA +2.4 14.2 two-component sensor protein (NCBI) compare
b1530 marR +1.7 2.2 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0464 acrR +1.5 9.8 DNA-binding transcriptional repressor (NCBI) compare
b1831 proQ +1.4 5.1 putative solute/DNA competence effector (NCBI) compare
b1627 rsxA +1.3 6.5 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1048 mdoG +1.2 7.6 glucan biosynthesis protein, periplasmic (NCBI) compare
b2078 baeS +1.2 2.7 sensory histidine kinase in two-component regulatory system with BaeR (NCBI) compare
b1936 intG +1.1 1.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1693 aroD +1.1 5.9 3-dehydroquinate dehydratase (NCBI) compare
b3390 aroK +1.1 2.8 shikimate kinase I (NCBI) compare
b1049 mdoH +1.1 8.4 glucosyltransferase MdoH (NCBI) compare
b4313 fimE +1.0 8.6 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4133 cadC +1.0 9.2 DNA-binding transcriptional activator (NCBI) compare
b1765 ydjA +1.0 1.4 predicted oxidoreductase (NCBI) compare
b4590 ybfK +1.0 1.5 hypothetical protein (RefSeq) compare
b1628 rsxB +1.0 4.3 electron transport complex protein RnfB (NCBI) compare
b3281 aroE +1.0 7.2 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2189 proL +0.9 3.5 tRNA-Pro (NCBI) compare
b2179 yejE +0.9 5.5 predicted oligopeptide transporter subunit (NCBI) compare
b3609 secB +0.9 2.8 export protein SecB (NCBI) compare
b1361 ydaW +0.9 0.8 orf, hypothetical protein (VIMSS) compare
b3027 ygiZ +0.9 0.8 conserved inner membrane protein (NCBI) compare
b3755 yieP +0.8 4.7 predicted transcriptional regulator (NCBI) compare
b3002 yqhA +0.8 3.4 hypothetical protein (NCBI) compare
b1630 rsxD +0.8 4.9 electron transport complex protein RnfD (NCBI) compare
b4585 sroB +0.8 1.9 Novel sRNA, function unknown (RefSeq) compare
b2502 ppx +0.8 6.0 exopolyphosphatase (NCBI) compare
b1848 yebG +0.8 2.2 conserved protein regulated by LexA (NCBI) compare
b4383 deoB +0.8 5.8 phosphopentomutase (NCBI) compare
b2178 yejB +0.8 4.9 predicted oligopeptide transporter subunit (NCBI) compare
b2945 endA +0.8 4.4 DNA-specific endonuclease I (NCBI) compare
b1257 yciE +0.8 0.4 hypothetical protein (NCBI) compare
b0929 ompF +0.7 5.1 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2180 yejF +0.7 5.4 fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI) compare
b4041 plsB +0.7 2.5 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1709 btuD +0.7 1.3 vitamin B12-transporter ATPase (NCBI) compare
b1632 rsxE +0.7 5.1 NADH-ubiquinone oxidoreductase (NCBI) compare
b1236 galU +0.7 2.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3146 yraL +0.7 5.7 predicted methyltransferase (NCBI) compare
b1090 plsX +0.7 2.4 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4603 rseX +0.7 0.8 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4402 yjjY +0.7 1.4 hypothetical protein (NCBI) compare
b2741 rpoS +0.7 4.5 RNA polymerase sigma factor (NCBI) compare
b2177 yejA +0.7 5.0 orf, hypothetical protein (VIMSS) compare
b2659 b2659 +0.7 2.2 orf, hypothetical protein (VIMSS) compare
b2346 vacJ +0.6 4.8 predicted lipoprotein (NCBI) compare
b3397 nudE +0.6 5.4 ADP-ribose diphosphatase (NCBI) compare
b3779 gpp +0.6 5.6 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b1346 ydaQ +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b1557 cspB +0.6 2.2 Qin prophage; cold shock protein (NCBI) compare
b3673 emrD +0.6 5.3 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b1382 ynbE +0.6 1.6 predicted lipoprotein (NCBI) compare
b3429 glgA +0.6 5.2 glycogen synthase (NCBI) compare
b2181 yejG +0.6 2.5 hypothetical protein (NCBI) compare
b3193 yrbD +0.6 4.6 predicted ABC-type organic solvent transporter (NCBI) compare
b3192 yrbC +0.6 4.8 predicted ABC-type organic solvent transporter (NCBI) compare
b0696 kdpC +0.6 3.1 potassium-transporting ATPase subunit C (NCBI) compare
b4426 isrA +0.6 2.0 unknown RNA (NCBI) compare
b1600 mdtJ +0.6 1.3 multidrug efflux system transporter (NCBI) compare
b1205 ychH +0.6 1.9 predicted inner membrane protein (NCBI) compare
b0730 mngR +0.6 1.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4381 deoC +0.6 2.5 deoxyribose-phosphate aldolase (NCBI) compare
b1363 trkG +0.6 0.5 Rac prophage; potassium transporter subunit (NCBI) compare
b4647 mokA +0.6 0.9 no description compare
b0759 galE +0.6 3.8 UDP-galactose-4-epimerase (VIMSS) compare
b1524 yneH +0.5 1.0 predicted glutaminase (NCBI) compare
b1400 paaY +0.5 1.4 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2214 yojL +0.5 4.1 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b1111 ycfQ +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b3133 agaV +0.5 1.5 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b1505 ydeT +0.5 1.1 hypothetical protein (RefSeq) compare
b1690 ydiM +0.5 1.7 predicted transporter (NCBI) compare
b1642 slyA +0.5 2.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1607 ydgC +0.5 1.5 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3500 gor +0.5 1.3 glutathione reductase (NCBI) compare
b1058 yceO +0.5 0.3 hypothetical protein (NCBI) compare
b3229 sspA +0.5 2.3 stringent starvation protein A (NCBI) compare
b1573 ydfC +0.5 1.4 Qin prophage; predicted protein (RefSeq) compare
b4246 pyrL +0.5 1.2 pyrBI operon leader peptide (NCBI) compare
b0661 miaB +0.5 4.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b4536 yobH +0.5 2.0 hypothetical protein (NCBI) compare
b1629 rsxC +0.5 3.7 electron transport complex protein RnfC (NCBI) compare
b3495 uspA +0.5 4.3 universal stress global response regulator (NCBI) compare
b1502 ydeQ +0.5 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b3403 pck +0.5 3.9 phosphoenolpyruvate carboxykinase (NCBI) compare
b4618 tisB +0.5 3.0 lexA-regulated toxic peptide (RefSeq) compare
b4214 cysQ +0.5 3.8 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0945 pyrD +0.5 2.2 dihydroorotate dehydrogenase (NCBI) compare
b2531 iscR +0.5 1.5 DNA-binding transcriptional repressor (NCBI) compare
b1954 dsrA +0.5 2.1 regulatory antisense RNA (NCBI) compare
b2960 yggH +0.5 3.0 tRNA(m7G46)-methyltransferase (NCBI) compare
b4557 yidD +0.5 2.0 hypothetical protein (NCBI) compare
b1284 yciT +0.5 2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1735 chbR +0.5 2.1 DNA-binding transcriptional dual regulator (NCBI) compare
b1576 ydfD +0.5 0.2 Qin prophage; predicted protein (NCBI) compare
b0989 cspH +0.5 1.5 stress protein, member of the CspA-family (NCBI) compare
b1497 b1497 +0.5 0.2 putative enzyme (VIMSS) compare
b2258 b2258 +0.5 1.1 putative transport/receptor protein (VIMSS) compare
b3195 yrbF +0.5 3.9 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1606 folM +0.5 2.9 short chain dehydrogenase (NCBI) compare
b3790 rffC +0.5 1.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b3389 aroB +0.4 3.2 3-dehydroquinate synthase (NCBI) compare
b2217 rcsB +0.4 3.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1818 manY +0.4 1.7 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1088 yceD +0.4 1.6 hypothetical protein (NCBI) compare
b4595 yciY +0.4 1.5 hypothetical protein (RefSeq) compare
b0065 yabI +0.4 2.4 conserved inner membrane protein (NCBI) compare
b3009 yghB +0.4 3.3 conserved inner membrane protein (NCBI) compare
b2911 ssrS +0.4 1.0 6S regulatory RNA (NCBI) compare
b1194 ycgR +0.4 2.0 protein involved in flagellar function (NCBI) compare
b1347 ydaC +0.4 0.2 Rac prophage; predicted protein (NCBI) compare
b1366 ydaY +0.4 1.9 Rac prophage; predicted protein (RefSeq) compare
b2570 rseC +0.4 3.2 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b2859 ygeO +0.4 1.2 hypothetical protein (RefSeq) compare
b1824 yobF +0.4 1.6 hypothetical protein (NCBI) compare
b1156 tfaE +0.4 2.2 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b0527 ybcI +0.4 2.1 conserved inner membrane protein (NCBI) compare
b1591 b1591 +0.4 1.4 putative oxidoreductase component (VIMSS) compare
b2846 yqeH +0.4 0.4 orf, hypothetical protein (VIMSS) compare
b4598 yncL +0.4 1.4 hypothetical protein (RefSeq) compare
b0707 ybgA +0.4 1.8 hypothetical protein (NCBI) compare
b0953 rmf +0.4 1.4 ribosome modulation factor (NCBI) compare
b2579 yfiD +0.4 1.7 pyruvate formate lyase subunit (NCBI) compare
b1919 yedO +0.4 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1258 yciF +0.4 0.7 hypothetical protein (NCBI) compare
b1583 ynfB +0.4 1.2 hypothetical protein (NCBI) compare
b1492 gadC +0.4 2.7 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b3191 yrbB +0.4 3.2 orf, hypothetical protein (VIMSS) compare
b0412 yajI +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1545 pinQ +0.4 0.1 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1253 yciA +0.4 1.3 predicted hydrolase (NCBI) compare
b2136 yohD +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1691 ydiN +0.4 1.9 predicted transporter (RefSeq) compare
b2300 yfcE +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b4387 ytjB +0.4 2.1 hypothetical protein (NCBI) compare
b1242 ychE +0.4 1.8 predicted inner membrane protein (NCBI) compare
b4592 yccB +0.4 1.5 hypothetical protein (RefSeq) compare
b2204 napH +0.4 2.2 quinol dehydrogenase membrane component (NCBI) compare
b2252 ais +0.4 2.2 hypothetical protein (NCBI) compare
b3432 glgB +0.4 3.2 glycogen branching enzyme (NCBI) compare
b4438 cyaR +0.4 1.8 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1821 b1821 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b2998 yghW +0.4 1.8 hypothetical protein (NCBI) compare
b1900 araG +0.4 1.8 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0377 sbmA +0.4 3.0 predicted transporter (NCBI) compare
b1648 b1648 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3753 rbsR +0.4 3.1 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0974 hyaC +0.4 1.2 hydrogenase 1, b-type cytochrome subunit (NCBI) compare
b2734 pphB +0.3 1.9 serine/threonine-specific protein phosphatase 2 (NCBI) compare
b2319 usg +0.3 2.2 hypothetical protein (NCBI) compare
b1180 ycgM +0.3 1.6 predicted isomerase/hydrolase (NCBI) compare
b2690 yqaB +0.3 2.4 predicted hydrolase (NCBI) compare
b0279 yagM +0.3 0.2 CP4-6 prophage; predicted protein (NCBI) compare
b2066 udk +0.3 2.1 uridine/cytidine kinase (VIMSS) compare
b1134 ymfB +0.3 1.4 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b3206 npr +0.3 2.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4045 yjbJ +0.3 1.5 predicted stress response protein (NCBI) compare
b3912 cpxR +0.3 2.7 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b3766 ilvL +0.3 1.3 ilvG operon leader peptide (NCBI) compare
b2343 b2343 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1167 ymgC +0.3 0.2 hypothetical protein (NCBI) compare
b3138 agaB +0.3 1.5 N-acetylgalactosamine-specific enzyme IIB component of PTS (NCBI) compare
b0706 ybfD +0.3 1.2 hypothetical protein (NCBI) compare
b1067 yceH +0.3 1.2 hypothetical protein (NCBI) compare
b1182 hlyE +0.3 0.3 hemolysin E (VIMSS) compare
b2642 yfjW +0.3 0.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0982 yccY +0.3 2.1 putative phosphatase (VIMSS) compare
b1689 b1689 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b3816 corA +0.3 2.8 magnesium/nickel/cobalt transporter (NCBI) compare
b1951 rcsA +0.3 0.8 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2445 yffN +0.3 2.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b0998 torD +0.3 0.7 chaperone protein TorD (NCBI) compare
b4409 blr +0.3 2.4 beta-lactam resistance membrane protein (NCBI) compare
b1499 ydeO +0.3 0.2 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1141 ymfG +0.3 1.8 e14 prophage; predicted excisionase (NCBI) compare
b4684 yqfG +0.3 2.3 expressed protein (RefSeq) compare
b2266 elaB +0.3 1.9 hypothetical protein (NCBI) compare
b3933 ftsN +0.3 2.7 essential cell division protein (NCBI) compare
b1108 ycfP +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b0645 ybeR +0.3 2.3 hypothetical protein (NCBI) compare
b1536 ydeI +0.3 0.4 hypothetical protein (NCBI) compare
b1823 cspC +0.3 1.2 stress protein, member of the CspA-family (NCBI) compare
b1742 b1742 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b0300 ykgA +0.3 1.5 putative ARAC-type regulatory protein (VIMSS) compare
b0937 ssuE +0.3 1.1 NAD(P)H-dependent FMN reductase (NCBI) compare
b2157 yeiE +0.3 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0310 ykgH +0.3 0.7 predicted inner membrane protein (NCBI) compare
b4407 thiS +0.3 1.0 sulfur carrier protein ThiS (NCBI) compare
b0909 ycaL +0.3 2.1 putative heat shock protein (VIMSS) compare
b1960 vsr +0.3 1.1 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1669 ydhT +0.3 1.2 hypothetical protein (NCBI) compare
b0660 ybeZ +0.3 1.7 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare


Specific Phenotypes

For 7 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms